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| 1 | Ti/(Ti,Cr)N/CrN multilayer coated 316L stainless steel by arc ion plating as bipolar plates for proton exchange membrane fuel cells显示文摘Arc ion plating(AIP) is applied to form Ti/(Ti,Cr)N/Cr N multilayer coating on the surface of 316 L stainless steel(SS316L) as bipolar plates for proton exchange membrane fuel cells(PEMFCs). The characterizations of the coating are analyzed by scanning electron microscopy(SEM) and X-ray diffraction(XRD). Interfacial contact resistance(ICR) between the coated sample and carbon paper is 4.9 m cm^2 under 150 N/cm^2,which is much lower than that of the SS316 L substrate. Potentiodynamic and potentiostatic tests are performed in the simulated PEMFC working conditions to investigate the corrosion behaviors of the coated sample. Superior anticorrosion performance is observed for the coated sample, whose corrosion current density is 0.12 μA/cm2. Surface morphology results after corrosion tests indicate that the substrate is well protected by the multilayer coating. Performances of the single cell with the multilayer coated SS316 L bipolar plate are improved significantly compared with that of the cell with the uncoated SS316 L bipolar plate, presenting a great potential for PEMFC application. | Shengli Wang Ming Hou Qing Zhao Yongyi Jiang Zhen Wang Huizhe Li Yu Fu Zhigang Shao | 2017 | Journal of Energy Chemistry2017，26，1： | 17 |
| 2 | Research Progress on Heat Stress of Rice at Flowering Stage显示文摘Global warming has caused frequent occurrence of heat stress at the flowering stage of single-season rice in the Yangtze River region of China, which results in declines of spikelet fertility and yield in rice. Rice flowering stage is the most sensitive period to high temperatures, and therefore, the key for heat stress happening is the flowering stage coinciding with high temperature, which causes spikelet fertility decreasing in heat-sensitive varieties, and is the major factor for heat injury differences among various rice planting regions. With the development of rice breeding, temperature indexes for heat stress has been converted from daily maximum temperature of 35 oC to 38 oC with the stress duration of more than 3 d. During the flowering stage, anther dehiscence inhibition and low pollen shedding onto the stigma are two main reasons for spikelet fertility reduction under high temperatures. At panicle initiation stage, high temperatures aggravate spikelet degeneration, and destroy floral organ development. Various types of rice varieties coexist in production, and indica-japonica hybrid rice demonstrates the highest heat resistance in general, followed by indica and japonica rice varieties. In production, avoiding high temperature is the main strategy of preventing heat stress, and planting suitable cultivars and adjustment of sowing date are the most effective measures. Irrigation is an effective real-time cultivation measure to decline the canopy temperature during the rice flowering stage. We suggested that further study should be focused on exploring heat injury differences among different rice variety types, and innovating rice-planting methods according to planting system changes in rice planting regions with extreme heat stress. Meanwhile, high temperature monitor and warning systems should be improved to achieve optimal heat stress management efficiencies. | WANG Yaliang WANG Lei ZHOU Jianxia HU Shengbo CHEN Huizhe XIANG Jing ZHANG Yikai ZENG Yongjun SHI Qinghua ZHU Defeng ZHANG Yuping | 2019 | Rice science2019，26，1： | 13 |
| 3 | APP and APLP1 are degraded through autophagy in response to proteasome inhibition in neuronal cells显示文摘Amyloid beta(Aβ)precursor protein(APP)is a key protein in the pathogenesis of Alzheimer’s disease(AD).Both APP and its paralogue APLP1(amyloid beta precursor-like protein 1)have multiple functions in cell adhesion and proliferation.Previously it was thought that autophagy is a novel beta-amyloid peptide(Aβ)-generating pathway activated in AD.However,the protein proteolysis of APLP1 is still largely unknown.The present study shows that APLP1 is rapidly degraded in neuronal cells in response to stresses,such as proteasome inhibition.Activation of the endoplasmic reticulum(ER)stress by proteasome inhibitors induces autophagy,causing reduction of mature APLP1/APP.Blocking autophagy or JNK stress kinase rescues the protein expression for both APP and APLP1.Therefore,our results suggest that APP/APLP1 is degraded through autophagy and the APLP1 proteolysis is mainly mediated by autophagy-lysosome pathway. | Fangfang Zhou Theo van Laar Huizhe Huang Long Zhang | 2011 | Protein & Cell2011，2，5： | 8 |
| 4 | Root morphology in response to nitrogen supply in mid-season indica rice cultivars released in different decades显示文摘Dear Editor,Roots,as a major organ of plants,are involved in nutrient and water acquisition,and might play a vital role in yield increase and efficient N absorption with genetic improvement.Because of the great differences in growth period and pattern between the old and new rice cultivars,it is difficult to clarify how genetic improvements contribute to root growth in rice.For example,for a solution culture system,Wu et al.suggested that total root length increased with increasing year of release for maize hybrids,while shoot dry weight, | Yikai Zhang Huizhe Chen Yuping Zhang Jing Xiang Guangmei Ji Defeng Zhu | 2017 | Science China(Life Sciences)2017，60，4： | 7 |
| 5 | Nusap1 is essential for neural crest cell migration in zebrafish显示文摘Microtubules play important roles in mitotic spindle assembly and chromosome segregation to maintain normal cell cycle progression.A number of microtubule-associated proteins have been identified in epithelial and neural cell cultures;however,their physiological significance is not well characterized due to the lack of appropriate in vivo animal models.Nucleolar spindleassociated protein(NuSAP)is a microtubule-binding protein and is reported to be involved in mitosis by cell culture studies.In this report,we identified the zebrafish homologue of human NuSAP and investigated its expression profile and functions.Using in situ hybridization,we demonstrated that transcripts of zebrafish nusap1 are specifically expressed in the retina,forebrain,hindbrain and neural crest.When the in vivo expression of nusap1 was knocked down through antisense oligonucleotide morpholino technology,the morphants of nusap1 showed impaired morphogenesis in the trunk and yolk extension,implying the involvement of Nusap1 in cell migration.Mechanistic studies revealed that nusap1 morphants have an altered expression pattern of neural crest markers crestin and sox9b,but normal expression of blood vessel and notochord markers gata1 and shh.In addition,nusap1 mRNA injection caused serious apoptosis in retina and hindbrain tissue,and these phenotypes can be rescued by co-injection of morpholino against nusap1.These observations not only suggest a role for Nusap1 in connecting apoptosis with cell migration,but also provide strong evidences that Nusap1 is potentially involved in morphogenesis in vertebrates. | Jing Nie Hua Wang Fuchu He Huizhe Huang | 2010 | Protein & Cell2010，1，3： | 7 |
| 6 | Decrement of Sugar Consumption in Rice Young Panicle Under High Temperature Aggravates Spikelet Number Reduction显示文摘Two rice genotypes Huanghuazhan(HHZ, heat-resistant) and IR36(heat-susceptible) were subjected to high-temperature(HT, 40℃) and normal-temperature(NT, 32℃) treatments at the spikelet differentiation stage. HT treatment inhibited spikelet differentiation, aggravated spikelet degeneration, reduced spikelet size, and disordered carbohydrate allocation. Meanwhile, HT treatment increased nonstructural carbohydrate content in leaves, but decreased that in stems and young panicles, and the same tendencies of sucrose and starch contents were observed in leaves and stem. However, HT treatment significantly increased the sucrose content and sharply decreased the glucose and fructose contents in young panicles. Lower activity levels of soluble acid invertase(EC3.2.1.26) and sucrose synthase(EC2.4.1.13) were observed under HT treatment. Moreover, HT treatment reduced the activities of key enzymes associated with glycolysis and the tricarboxylic acid cycle, which indicated sucrose consumption was inhibited in young panicles under HT treatment. Exogenous glucose and fructose applied under HT treatment increased the spikelet number more than exogenous sucrose. In conclusion, the results demonstrated that the reduction of spikelet number under high temperature was more affected by the decrease in sugar consumption than the blocking of sucrose transport. The impairment of sucrose hydrolysis was the main reason for the inhibition of sugar utilization. | WANG Yaliang ZHANG Yikai SHI Qinghua CHEN Huizhe XIANG Jing HU Guohui CHEN Yanhua WANG Xiaodan WANG Junke YI Zihao ZHU Defeng ZHANG Yuping | 2020 | Rice science2020，27，1： | 6 |
| 7 | Bisindoylmaleimide I enhances osteogenic differentiation显示文摘The Wnt/β-catenin and bone morphogenetic proteins(BMPs)pathways play important roles in controlling osteogenesis.Using a cell-based kinase inhibitor screening assay,we identified the compound bisin-doylmaleimide I(BIM)as a potent agonist of the cyto-solicβ-catenin accumulation in preosteoblast cells.Through suppressing glycogen synthase kinase 3βenzyme activities,BIM upregulatedβ-catenin respon-sive transcription and extended duration of BMP initi-ated signal.Functional analysis revealed that BIM promoted osteoblast differentiation and bone formation.The treatment of human mesenchymal stem cells with BIM promoted osteoblastogenesis.Our findings pro-vide a new strategy to regulate mesenchymal stem cell differentiation by integration of the cellular signaling pathways. | Fangfang Zhou Huizhe Huang Long Zhang | 2012 | Protein & Cell2012，3，4： | 2 |
| 8 | Suppression of GSK3β by ERK mediates lipopolysaccharide induced cell migration in macrophage through β-catenin signaling显示文摘We investigate the role of β-catenin signaling in the response of macrophage to lipopolysaccharide(LPS)using RAW264.7 cells.LPS rapidly stimulated cytosolic β-catenin accumulation.β-catenin-mediated transcription was showed to be required for LPS induced gene expression and cell migration.Mechanically,ERK activation-primed GSK3β inactivation by Akt was demonstrated to mediate the LPS induced β-catenin accumulation.Overall,our findings suggest that suppression of GSK3β by ERK stimulatesβ-catenin signaling therefore contributes to LPS induced cell migration in macrophage activation. | Kai Gong Fangfang Zhou Huizhe Huang Yandao Gong Long Zhang | 2012 | Protein & Cell2012，3，10： | 2 |
| 9 | Synthesis, photostability and bioactivity of 2,3-cyclopropanated abscisic acid显示文摘 | Liu Wenjian Han Xiaoqiang Xiao Yumei Fan Jinlong Zhang Yuanzhi Lu Huizhe Wang Mingan Qin Zhaohai | 2013 | Phytochemistry2013，，： | 1 |
| 10 | Technical analysis of bridge loading test显示文摘 | Shi Huizhe | 2012 | East China Science&Technology2012，，4： | 1 |
| 11 | Identification of rare PTCH1 nonsense variant causing orofacial cleft in a Chinese family and an up-to-date genotype- phenotype analysis显示文摘The Patched 1(PTCH1)gene encodes a membrane receptor involved in the Hedge-hog(Hh)signaling pathway,an abnormal state of which may result in congenital defects or hu-man tumors.In this study,we conducted whole-exome sequencing on a three-generation Chinese family characterized with variable penetrance of orofacial clefts.A rare heterozygous variant in the PTCH1 gene(c.2833C>T p.R945X)was identified as a disease-associated mutation.Structural modeling revealed a truncation starting from the middle of the second extracellular domain of PTCH1 protein.This may damage its ligand recognition and sterol transportation abilities,thereby affecting the Hh signaling pathway.Biochemical assays indi-cated that the R945X protein had reduced stability compared to the wild-type in vitro.In addi-tion,we reviewed the locations and mutation types of PTCH1 variants in individuals with clefting phenotypes,and analyzed the associations between clefts and locations or types of variants within PTCH1.Our findings provide further evidence that PTCH1 variants result in or-ofacial clefts,and contributed to genetic counseling and clinical surveillance in this family. | Wenjie Zhong Huaxiang Zhao Wenbin Huang Mengqi Zhang Qian Zhang Yue Zhang Chong Chen Zulihumaer Nueraihemaiti Dilifeire Tuerhong Huizhe Huang Gulibaha Maimaitili Feng Chen Jiuxiang Lin | 2021 | Genes & Diseases2021，8，5： | 1 |
| 12 | Interference-induced broadband absorption enhancement for plasmonic-metal@ semiconductor microsphere as visible light photocatalyst显示文摘 | SUN Song LIU Huizhe WU Lin | 2014 | Catal2014，4，12： | 1 |
| 13 | Analysis of the intestinal flora in patientswith primary liver cancer显示文摘Objective To investigate the differences in intestinal flora of patients with primary liver cancer and ofhealthy individuals and to investigate the effect of the differential flora on the development of liver cancer.Methods Overall, 67 patients with primary liver cancer who received systematic and complete treatmentbetween January 2019 and December 2020 at the Sixth People’s Hospital of Qingdao and had completeclinical data were enrolled in this study, and 26 individuals who were healthy on physical examination inthe same period were used as healthy controls. Macro genome and 16s ribosome Deoxyribo Nucleic Acid(rDNA) high-throughput sequencing were performed on the stool flora of the enrolled patients and controls,and the differences in the intestinal flora were analyzed using the LEfSe bioinformatics software.Results Compared with the control samples, all the tested patient samples showed statistically significantdifferences in the number of colonies of 5 bacterial phyla, 5 orders, 8 families, 11 genera, and 14 species(P < 0.05).Conclusion Compared with healthy people, patients with primary liver cancer have significant differencesin the intestinal flora composition. The alteration of the intestinal flora may be correlated with the occurrenceof primary liver cancer, and the intestinal flora may become a novel target for the prevention and treatmentof primary liver cancer. | Chengcong Liu Guoxin Sun Huizhe Wang Gaishuang Shang Xiong Yan Xiao Zou | 2023 | Oncology and Translational Medicine2023，9，1： | 0 |
| 14 | Overlapped Tray Seedling Raising Model for Mechanical Transplanting of Rice显示文摘With the development of China's social economy,transfer of rural labors,and rise of labor costs,the rice production technologies are changing from manual operation to mechanical operation.The core of mechanical rice production is mechanical planting,while the key to mechanical planting is the seedling raising.On the basis of analyzing and studying the problems and experience of traditional mechanical transplanting of rice,a new overlapped tray seedling raising model was introduced.Main features of this model: substrate seedling raising and overlapped seedling tray.The seedling trays for seeding line are overlapped,and the overlapped trays are moved into the seedling room with controlled temperature and humidity.The temperature of the seedling room is controlled at 30-32℃,and the humidity of the seedling room is controlled at above 90%.After about 48 h,when the seedling height reaches 0.5 cm,move the seedling trays to the nursery.This model consists of one seeding center( seedling raising center) and N nurseries,to realize 1 + N seedling raising for mechanical transplanting of rice.One seeding center can provide seedlings for several hundred to several thousand hectares of mechanical transplanting of rice,and provide a new model for social services.This model could improve the quality of seedlings,increase the seedling survival rate by about 20%,reduce the seedling raising costs by 15%-20%,reduce the seedling raising risks,and greatly increase the utilization rate of the site and equipment of seedling raising center. | Defeng ZHU Yuejun WANG Huizhe CHEN Yicheng XU Yiping CHEN | 2019 | Asian Agricultural Research2019，11，1： | 0 |
| 15 | Research Progress of Hair Growth Technology显示文摘This summarizes the research progress of domestic and foreign experts on the methods and technologies of hair growth,including the hair growth effects of natural plant extracts,mesotherapy and stem cell therapy.Looking forward to the development trend in the field of hair growth,mesotherapy has the advantages of convenient operation,good targeting,fewer side effects,and high cost performance.Combining it with anti-hair loss cosmetic products containing natural plant extracts to treat hair loss has very broad application prospects. | Zhang Jingjing Xia Liming Meng Xiangyan Shi Huizhe | 2021 | China Detergent & Cosmetics2021，6，3： | 0 |
| 16 | Correlation analysis of breast fibroadenoma and the intestinal flora based on 16S rRNA sequencing显示文摘Objective To analyze the characteristics of the intestinal microflora in patients with breast fibroadenoma using 16S ribosomal RNA(rRNA)high-throughput sequencing.Methods Fecal samples from 20 patients with breast fibroadenoma and 36 healthy subjects were randomly collected and analyzed using high-throughput sequencing technology for 16S rRNA V4 region sequencing,and the alpha diversity(Chao index,Shannon index)was calculated using Mothur(v.1.39.5)software.Beta diversity was analyzed using QIIME(v1.80).SPSS software(version 23.0)and the t-test of two independent samples were used to analyze differences in the abundance of bacteria between the two groups.Results Compared with that in the healthy control group,theαdiversity of the intestinal microflora in breast fibroadenoma patients increased,but the difference was not statistically significant(P>0.05).At the phylum level,significant differences were observed between the two groups.The abundance of Firmicutes was higher in the breast fibroadenoma group(P<0.05),whereas the abundance of Synergistetes was higher in the healthy control group(P<0.005).A total of five bacterial genera showed significant differences between the two groups:the breast fibroadenoma group showed higher levels of Bautia(P<0.005),Coprococcus(P<0.005),Roseburia(P<0.05),and Ruminococcus(P<0.005),whereas Sutterella was more abundant in the healthy control group than in the breast fibroadenoma group(P<0.05).Conclusion The diversity and abundance of the intestinal flora in patients with breast fibroadenoma are significantly different from those in healthy subjects,suggesting that an imbalance in the intestinal flora is correlated with the occurrence of breast fibroadenoma. | Bingdong Wang Xin Liu Yahong Bian Guoxin Sun Huizhe Wang Jingjin Zhang Zhengfu Zhang Xiao Zou | 2021 | Oncology and Translational Medicine2021，7，6： | 0 |
| 17 | Docking process on hybrid self-reconfigurable modular robots显示文摘A novel hybrid self-reconfigurable modular robot is designed to finish the morphing action from line shape to hexagon shape. The robot is composed of many basic modules,each of which consists of a master module and a slave module in the shape of triangular prism. There are four connection ports on each basic module. For the master module there are two holes on each connection port,and for the slave one there are two pegs on each connection. The docking process between two neighboring basic modules is analyzed with a peg-in-hole mechanical structure. A small motion's method is presented and the contact forces are derived. According to the force/moment,the pose of a motion module should be adjusted to make two neighboring modules align and finish the docking process.Finally,a simulation of 3 basic modules is shown to finish the morphing and docking process effectively. The system can finish the morphing task from the line shape to the hexagon shape. | 帅立国 Su Huizhe Zheng Liyuan Fei Yanqiong | 2017 | High Technology Letters2017，23，1： | 0 |
| 18 | Brassinosteroids Mediate Endogenous Phytohormone Metabolism to Alleviate High Temperature Injury at Panicle Initiation Stage in Rice显示文摘High temperatures cause physiological and biochemical changes and significantly affect young panicle development of rice(Oryza sativa L.).Brassinosteroids play important roles in enhancing crop stress resistance.In this study,we subjected rice cultivars Huanghuazhan(heat-resistant)and IR36(heat-sensitive)to high temperature(HT,40 oC)or normal temperature(NT,33 oC)for 7 d at the panicle initiation stage,in conjunction with application of 24-epibrassinolide[EBR,a synthetic brassinolide(BR)]or brassinazole(BRZ,a BR biosynthesis inhibitor)at the beginning of the treatments.HT exacerbated spikelet degeneration and inhibited young panicle growth,which were partially prevented by EBR application,while BRZ application aggravated the reduction in spikelet number.HT decreased the contents of BR,active cytokinins(aCTK),active gibberellins(aGA)and indole-3-acetic acid(IAA),but increased the content of abscisic acid(ABA)in young panicles.The activities of key enzymes involved in sucrose hydrolysis,glycolysis and the tricarboxylic acid cycle in young panicles were decreased with the change of endogenous hormone levels under HT.In addition,the contents of H2O2 and malondialdehyde(MDA)were increased and the activities of antioxidant enzymes were decreased in young panicles.Exogenous application of EBR induced the expression of phytohormone biosynthesis-related genes and down-regulated the expression of phytohormone catabolism-related genes to increase the contents of endogenous BR,aCTK,aGA and ABA,thus promoting the decomposition and utilization of sucrose in young panicles,enhancing the activities of superoxide dismutase,catalase and peroxidase,and reducing the accumulation of H2O2 and MDA in young panicles,whereas application of BRZ had the opposite physiological effects.These results showed that brassinosteroids mediate endogenous phytohormone metabolism to alleviate HT injury at the panicle initiation stage in rice. | CHEN Yanhua WANG Yaliang CHEN Huizhe XIANG Jing ZHANG Yikai WANG Zhigang ZHU Defeng ZHANG Yuping | 2023 | Rice science2023，30，1： | 0 |
| 19 | Peripheral nerve allograft prepared by an acellular,hypotonic tissue engineering method显示文摘BACKGROUND:Tissue engineered acellular nerves are good autologous nerve substitutes.Acellular peripheral nerves prepared using a conventional chemical extraction method cause a great deal of damage to nerve structures,and the allograft affects the nerve regeneration following transplantation.OBJECTIVE:To prepare peripheral nerve grafts through an acellular tissue engineering method,and observe their histology,ultrastructure,protein components and histocompatibility.DESIGN,TIME AND SETTING:A randomized,controlled,in vivo nerve tissue engineering experiment was performed at the Department of Biochemistry and Molecular Biology,Shenyang Medical College,China,from September 2006 to June 2007.MATERIALS:Triton X-100,Pepstatin A,Aprotinin and Leupeptin were purchased from Sigma,USA;Tris(hydroxymethyl) aminomethane was purchased from Gibco,USA.METHODS:The bilateral sciatic nerves of Wistar rats were harvested,treated with 0.05 mol/L Tris-HCl buffer,followed by proteinase inhibitor and Triton X-100 to prepare acellular peripheral nerves.The nerves were implanted in the quadriceps femoris muscle of healthy Wistar rats.MAIN OUTCOME MEASURES:Tissue structure and ultrastructure of acellular peripheral nerves were observed by optical microscopy and scanning electron microscopy.Growth associated proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.Nerve allograft and the surrounding muscles were observed by hematoxylin-eosin staining.RESULTS:Acellular treatment eliminated Schwann cells,epineurium or perineurium cells,myelin sheaths and axons of nerve fibers in normal peripheral nerves,while the spatial structure,comprising basement membrane tubes of Schwann cells and the extracellular matrix of perineurium and nerve fascicles was maintained.Protein bands at the region of 30 kD were no longer visible,had slightly decreased at 43 kD and remained unchanged at 65 kD.Following implantation for 7 days,epineurium cells were absorbed.However,increased fibroblasts,decreased newly-generated capillaries and maturation of granulation tissue were observed.CONCLUSION:The acellular nerve allograft prepared through the use of a hypotonic,acellular method displays good histocompatibility,eliminates immune substances and retains growth associated proteins that induce the growth of the neural axis.In addition,this method provides an ideal scaffold to construct artificial nerves. | Huizhe Sun Wenying Dai Jie Wang Xiaojie Tong Wei Tian Jun Xu | 2010 | Neural Regeneration Research2010，5，8： | 0 |
| 20 | Wind and Snow Impacts on Urban Space Planning:A Multiphase Numerical Simulation显示文摘The wind and snow environment outside the planned space plays a key role in the comfort and safety of the human habitat in severe cold regions. Traditional studies of the external environment of human settlements, however, frequently overlook the combined impacts of wind and snow environments. Furthermore, in urban meteorological studies, it is impossible to accurately assess the wind and snow environment in specific areas or locations. In this study, a refined Computational Fluid Dynamics(CFD) multiphase flow numerical method was used to simulate a planning space's wind and snow environment. The study classified the Snowstorm Weather Grade(SWG) by incorporating the Snowstorm Weather Index(SWI) to generate calculation results of the wind environment and snow environment. In particular, 150 measurement points in the planning space were chosen for analysis and evaluation of their wind and snow environments. The results demonstrated that the SWI index can effectively correlate to the wind and snow environment calculation results. In addition, the graph of SWI showed that 55% of the measurement points had a moderate wind and snow grade SWI, which exceeds the average grade for the entire region. The practical application shows that the wind and snow environment assessment indexes and technical methods developed in this paper can be successfully applied to wind and snow environment studies in other cold cities. | Zhihao Hu Huizhe Cao Bin Zhang Chein-Chi Chang Biao Li | 2023 | Journal of Harbin Institute of Technology(New Series)2023，30，5： | 0 |
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