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| 1 | Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii显示文摘The expression vector containing phbB and ble genes was constructed and transformed into cell-wall- deficient strain Chlamydomonas reinhardtii CC-849 by the glass-bead method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mg/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis. | WANGChaogang HUZhangli HUWei LEIAnping | 2004 | Chinese Science Bulletin2004，49，16： | 7 |
| 2 | NIR-IIb excitable bright polymer dots with deep-red emission for in vivo through-skull three-photon fluorescence bioimaging显示文摘It is of great significance to study the brain structure and function in deep-tissue for neuroscience research and bio-medical applications because of the urgent demand for precise theranostics.Three-photon fluorescence microscopic(3PFM)bioimaging excited by the light in near-infrared IIb(NIR-IIb,1,500–1,700 nm)spectral region is one of the most promising imaging techniques with the advantages of high spatial resolution,large imaging depth,and reduced scattering.Herein,a type of NIR-IIb light excitable deep-red emissive semiconducting polymer dots(P-dots)with bright 3PF and large three-photon absorption cross-section(σ3)at 1,550 nm was prepared.Then the P-dots were functionalized with polystyrene polymer polystyrene graft ethylene oxide functionalized with carboxyl groups(PS-PEG-COOH)and modified with NH2-poly(ethylene glycol)(PEG)to synthesis photochemically stable and biocompatible P-dots nanoparticles(NPs).Further the P-dots NPs were utilized for in vivo 3PFM bioimaging of cerebral vasculature with and without the brain skull under 1,550 nm femtosecond(fs)laser excitation.In vivo 3PFM bioimaging of the mice cerebral vasculature at various vertical depths was obtained.Moreover,a vivid three-dimensional structure of the mice vascular architecture beneath the skull was reconstructed.At the depth of 350μm beneath the brain skull,3.8μm blood vessels could still be clearly recognized.NIR-IIb excitable P-dots assisted 3PFM bioimaging has great potential in accurate deep tissue bioimaging. | Nuernisha Alifu Abudureheman Zebibula Hequn Zhang Huwei Ni Liang Zhu Wang Xi Yalun Wang Xueliang Zhang Changfeng Wu Jun Qian | 2020 | Nano Research2020，13，10： | 6 |
| 3 | Evaluation of seed production in a first-generation seed orchard of Chinese pine (Pinus tabuliformis)显示文摘We evaluated seed production in a first-generation orchard of Chinese pine(Pinus tabuliformis) during the crucial transition period from first generation to advanced generations.Clones varied significantly in all traits related to seed production.Repeatability of these traits ranged from 0 to 0.96.Seed production per ramet(SPPR),seed producing index(SPI),the number of relative female strobili(RFS),the number of scales,and the number of ineffective scales had comparatively high repeatability at 0.86,0.87,0.89,0.96,and 0.91,respectively.Correlation analysis showed that SPPR was greatly influenced by RFS and by the number of full seeds per cone.Finally,we showed that SPI was the best predictor of the seed-producing ability of clones.Our findings will assist seed orchard managers in effectively predicting and improving seed production of Chinese pine seed orchards. | Huwei Yuan Shihui Niu Xianqing Zhou Qingping Du Yue Li Wei Li | 2016 | Journal of Forestry Research2016，27，5： | 4 |
| 4 | Fragmentation study of two brassinolides by ion trap tandem mass spectrometry显示文摘The fragmentation pathways of two isomers of brassinosteroids,28-homobrassinolide(28-h-BL)and 28-epihomobrassinolide(28-eh-BL),have been investigated by tandem mass spectrometry(MSn,n=1,2,3,4)with the electrospray ionization(ESI) source.The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated,and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers. | HUO FeiFeng BAI Yu LIU HuWei | 2010 | Chinese Science Bulletin2010，55，21： | 4 |
| 5 | The genetic architecture of growth traits in Salix matsudana under salt stress显示文摘Willow(Salix)is one of the most important ornamental tree species in landscape plants.One species,Salix matsudana,is widely used as a shade tree and border tree because of its soft branches and plump crown.Some varieties of S.matsudana were salt tolerant and could grow normally in coastal regions.However,the molecular mechanisms of salt tolerance for S.matsudana have been less clear.Here,we addressed this issue by performing a mapping experiment containing 195 intraspecific F1 progeny of S.matsudana,derived from salt-sensitive‘yanjiang’and salt-tolerant‘9901’,grown by cuttings in a 100 mM NaCl solution.Growth performance of these progeny under salt stress was investigated,displaying marked genotypic variability with the coefficients of variance of 28.64–86.11%in shoot and root growth traits.We further mapped specific QTLs contributing to these differences to the Salix genome.Of the 204 QTLs identified,a few were detected to explain a remarkably larger portion of the phenotypic variation than many others.Many detected QTLs were found to reside in the region of candidate genes of known biological function.The discovery of growth QTLs expressed under salt stress provides important information for marker-assisted breeding of salt tolerant Salix varieties and founds the basis for the application of S.matsudana in coastal afforestation. | Jian Zhang Huwei Yuan Qingshan Yang Min Li Ying Wang Yujuan Li Xiangjian Ma Feng Tan Rongling Wu | 2017 | Horticulture Research2017，4，1： | 3 |
| 6 | Quantum dots assisted in vivo two-photon microscopy with NIR-Ⅱemission显示文摘With the advantages of high resolution and deep penetration depth,two-photon excited NIR-Ⅱ(900–1880 nm)fluorescence(2PF)microscopic bioimaging is promising.However,due to the lack of imaging systems and suitable probes,few such works,to our best knowledge,were demonstrated utilizing NIR-II excitation and NIR-Ⅱfluorescence simultaneously.Herein,we used aqueously dispersible Pb S/Cd S quantum dots with bright NIR-II fluorescence as the contrast agents.Under the excitation of a 1550 nm femtosecond(fs)laser,they emitted bright 2 PF in the NIR-Ⅱregion.Moreover,a 2PF lifetime imaging microscopic(2PFLIM)system was implemented,and in vivo 2PFLIM images of mouse brain blood vessels were obtained for the first time to our best knowledge.To improve imaging speed,an in vivo two-photon fluorescence microscopy(2 PFM)system based on an In Ga As camera was implemented,and in vivo 2PFM images of QDs-stained mouse brain blood vessels were obtained. | HUWEI NI YALUN WANG TAO TANG WENBIN YU DONGYU LI MUBIN HE RUNZE CHEN MINGXI ZHANG JUN QIAN | 2022 | Photonics Research2022，10，1： | 3 |
| 7 | Genome sequencing and phylogenetic analysis of allotetraploid Salix matsudana Koidz显示文摘Polyploidy is a common phenomenon among willow species.In this study,genome sequencing was conducted for Salix matsudana Koidz(also named Chinese willow),an important greening and arbor tree species,and the genome of this species was compared with those of four other tree species in Salicaceae.The total genome sequence of S.matsudana was 655.72 Mb in size,with repeated sequences accounting for 45.97%of the total length.In total,531.43 Mb of the genome sequence could be mapped onto 38 chromosomes using the published genetic map as a reference.The genome of S.matsudana could be divided into two groups,the A and B genomes,through homology analysis with the genome of Populus trichocarpa,and the A and B genomes contained 23,985 and 25,107 genes,respectively.4DTv combined transposon analysis predicted that allotetraploidy in S.matsudana appeared~4 million years ago.The results from this study will help reveal the evolutionary history of S.matsudana and lay a genetic basis for its breeding. | Jian Zhang Huwei Yuan Yujuan Li Yanhong Chen Guoyuan Liu Meixia Ye Chunmei Yu Bolin Lian Fei Zhong Yuna Jiang Jichen Xu | 2020 | Horticulture Research2020，7，1： | 3 |
| 8 | Generalized Consensus of Discrete-Time Multi-Agent Systems with Directed Topology and Communication Delay显示文摘Compared with traditional consensus,this paper studies the generalized consensus problem for discrete-time multi-agent systems with directed topology and communication delay.Novel distributed consensus protocols with and without communication delay are designed.Based on the analysis of error dynamical system and graph theory,the generalized consensus is globally asymptotically achieved under suitable conditions without changing the zero row-sums property of Laplacian matrix in networks.Moreover,the sufficient conditions for generalized consensus of communication delay are obtained under directed connections.Finally,some simulations have been provided to verify the theoretical results. | LIU Huwei CHEN Xin GUO Liuxiao HU Aihua | 2020 | Journal of Systems Science & Complexity2020，33，6： | 2 |
| 9 | Strigolactones interact with other phytohormones to modulate plant root growth and development显示文摘Strigolactones(SLs),which are biosynthesized mainly in roots,modulate various aspects of plant growth and development.Here,we review recent research on the role of SLs and their cross-regulation with auxin,cytokinin,and ethylene in the modulation of root growth and development.Under nutrientsufficient conditions,SLs regulate the elongation of primary roots and inhibit adventitious root formation in eudicot plants.SLs promote the elongation of seminal roots and increase the number of adventitious roots in grass plants in the short term,while inhibiting lateral root development in both grass and eudicot plants.The effects of SLs on the elongation of root hairs are variable and depend on plant species,growth conditions,and SL concentration.Nitrogen or phosphate deficiency induces the accumulation of endogenous SLs,modulates root growth and development.Genetic analyses indicate cross-regulation of SLs with auxin,cytokinin,and ethylene in regulation of root growth and development.We discuss the implications of these studies and consider their potential for exploiting the components of SL signaling for the design of crop plants with more efficient soil-resource utilization. | Huwei Sun Weiqiang Li David J.Burritt Hongtao Tian Heng Zhang Xiaohan Liang Yuchen Miao Mohammad Golam Mostofa Lam-Son Phan Tran | 2022 | The Crop Journal2022，10，6： | 2 |
| 10 | Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid显示文摘Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized. | YUE Hao CHAN Wan YU KongJie GUO Lin LIU Ning LIU HuWei CAI ZongWei | 2009 | Science China Chemistry2009，52，10： | 2 |
| 11 | Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation显示文摘Supercritical fluid chromatography(SFC),which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase,can provide fast,green,high-throughput and good performance methods for the analysis of complicated samples.It is an advantageous alternative for the separation of polar and nonpolar compounds,and even chiral isomers.As an important constituent of living organisms,lipids and the related lipidomics researches have attracted dramatic attention during the past years.The separation and identification of lipids and lipid isomers are of great significance and remain challenging.In this mini-review,the principle of SFC and its applications in lipid isomer separation are summarized. | Shiyao Song Huwei Liu Yu Bai | 2017 | Journal of Analysis and Testing2017，1，4： | 2 |
| 12 | Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice显示文摘 | Yu Bai Jialing Zhang Yu Bai Huwei Liu | 2012 | Analytical and Bioanalytical Chemistry2012，，： | 1 |
| 13 | Analysisoftheeffectofairpollutionontheadult''''srespiratoryhealth[J]显示文摘 | WUGuoping(吴国平) HUWei(胡伟) TENGEnjiang(滕恩江) etal | | 中国环境监测0，，： | 1 |
| 14 | Aggregation-induced emission luminogen for in vivo three-photon fuorescence lifetime microscopic imaging显示文摘Compared with visible light,near infrared(NIR)light has deeper penetration in biological tisues.Three-photon fuorescence microscopy(3PFM)can effectively utilize the NIR excitation to obtain high-contrast images in the deep tisue.However,the weak three photon fluorescence signals may be not well presented in the traditional fuorescence intensity imaging mode.Fluorescence lifetime of certain probes is insensitive to the intensity of the excitation laser.Moreover,fluorescence lifetimne imaging microscopy(FLIM)can detect weak signals by utilizing time correlated single photon counting(TCSPC)technique.Thus,it would be an improved strategy to combine the 3PFM imaging with the FLIM together.Herein,DCDPP-2TPA,a novel agegation-induced emission luminogen(AIEgen),was adopted as the fluorescent probes.The three-photon absorption cros-section of the AlEgen,which has a deep-red fluorescence emission,was proved to be large.DCDPP-2TPA nanoparticles were synthesized,and the three photon fluorescence lifetime of which was measured in water.Moreover,in vrivo thre-photon fuorescence lifetime microscopic imaging of a craniotomy mouse was conducted via a home made optical system.High contrast cerebrovascular images of different vertical depths were obtained and the maximun depth was about 600 pumn.Even reaching the depth of 600 pum,tiny capillary vessels as small as 1.9 pum could still be distinguished.The three photon fuorescence lifetimes of the capillaries in some representative images were in accord with that of DCDPP-2TPA nanoparticles in water.A vivid 3D reconstruction was further organized to present a wealth of lifetime information.In the future,the combination strategy of 3PFM and FLIM could be further applied in the brain functional imaging. | Huwei Ni Zicong Xu Dongyu Li Ming Chen Ben Zhong Tang Jun Qian | 2019 | Journal of Innovative Optical Health Sciences2019，，5： | 1 |
| 15 | Analysis of Chlorogenic Acid Traditional Chinese Medicines with Capillary Zone Electrophoresis显示文摘 | Long Hong Yang Jinjie Liu Huwei | 1999 | Journal of Chinese Pharmaceutical Scieneces1999，8，3： | 1 |
| 16 | Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids显示文摘 | Shehong Li Chiyang He Huwei Liu Kean Li Feng Liu | 2005 | Journal of Chromatography B2005，，1： | 1 |
| 17 | A rapid method for determination of Staphylococcus aureus based on milk coagulation by using a series piezoelectric quartz crystal sensor显示文摘 | Lili Bao Huwei Tan Qiong Duan Xiaoli Su Wanzhi Wei | 1998 | Analytica Chimica Acta1998，，1： | 1 |
| 18 | Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt显示文摘 | Chiyang He Shehong Li Huwei Liu Kean Li Feng Liu | 2005 | Journal of Chromatography A2005，，2： | 1 |
| 19 | Analysis of tetrandrine and fangchinoline in traditional Chinese medicines by capillary electrophoresis 显示文摘 | Yang Jinjie Long Hong Liu Huwei | 1998 | Journal of Chromatography A1998，811，： | 1 |
| 20 | Determination of synthetic colourant food aditives by capillary zone electrophoresis显示文摘 | Huwei Liu Tao Zhu | 1995 | Journal of Chromatography1995，718，： | 1 |
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