|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Currency crashes in emerging markets: An empirical treatment显示文摘 | Jeffrey A. Frankel Andrew K. Rose | 1996 | Journal of International Economics1996，，3： | 3 |
| 3 | Currency Crashes in Emerging Markets: An Empirical Treatment 显示文摘 | Frankel Jeffrey and Andrew Rose | 1996 | Journal of International Economics1996，41，： | 1 |
| 4 | Quantitative Bioanalytical Methods Validation and Implementation: Best Practices for Chromatographic and Ligand Binding Assays显示文摘 | C. T. Viswanathan Surendra Bansal Brian Booth Anthony J. DeStefano Mark J. Rose Jeffrey Sailstad Vinod P. Shah Jerome P. Skelly Patrick G. Swann Russell Weiner | 2007 | Pharmaceutical Research2007，，10： | 1 |
| 5 | Residue-free sodium phosphate tablets (OsmoPrep) versus Visicol for colon cleansing: a randomized, investigator-blinded trial显示文摘 | Lawrence Wruble Michael DeMicco Jeffrey Medoff Alan Safdi Jeffrey Bernstein Douglas Dalke Martin Rose Robyn G. Karlstadt Nancy Ettinger Bing Zhang | 2007 | Gastrointestinal Endoscopy2007，，4： | 1 |
| 6 | The Endogeneity of the Optimum Currency Area Criteria显示文摘 | Frankel Jeffrey Rose A.K | | 0，，108： | 1 |
| 7 | The Endogeneity of the Optimum Currency Area Criteria显示文摘 | Frankel Jeffrey and Andrew Rose | 1998 | The Economic Journal1998，108，449： | 1 |
| 8 | Is EMU more justifiable ex post than ex ante?显示文摘 | Jeffrey A. Frankel Andrew K. Rose | 1997 | European Economic Review1997，，3： | 1 |
| 9 | The endogeneity of the optimum currency area criteria 显示文摘 | JEFFREY A FRANKEL ANDREW K ROSE | 1998 | Economic Journal1998，，449： | 1 |
| 10 | The extra domain A of fibronectin activates Toll-like receptor 4显示文摘 | Okamura Y Watari M Jerud ES Sally T Ishizaka Jeffrey Rose | 2001 | J Biol Chem2001，276，10： | 1 |
| 11 | The Endogeneity of the Optimum Currency Area Criteria显示文摘 | Frankel Jeffrey A & Rose Andrew K | 1998 | Economic Journal1998，108，449： | 1 |
| 12 | Currency Crashes in Emerging Markets:An Empirical Treatment显示文摘 | JEFFREY A FRANKEL ANDREW K ROSE | 2001 | Journal of International Economics2001，，41： | 1 |
| 13 | The Endogeneity of the Optimum Currency Area Criteria 显示文摘 | Frankel Jeffrey A and Rose Andrew K | 1998 | Economic Journal Royal Economic Society1998，108，449： | 1 |
| 14 | Currency Crashes in Emerging Market: An Empirical Treatment 显示文摘 | Frankel Jeffrey Andrew Rose | 1996 | Journal of International Economic1996，41，11： | 1 |
| 15 | Currency Crashes in Emerging Markets: An Empirical 显示文摘 | Frankel Jeffrey and Rose Andrew 1996 | 1996 | Treatment Journal of International Economics1996，，41： | 1 |
| 16 | Currency crashes in emerging markets:An empirical treatment显示文摘 | Frankel Jeffrey A Rose Andrew K | 1996 | Journal of International Economics1996，41，3： | 1 |
| 17 | The Endogeneity of the Optimum Currency Area Criteria 显示文摘 | Frankel Jeffrey and Andrew Rose | 1998 | The Economic Journal1998，108，449： | 1 |
| 18 | Workshop/conference report—Quantitative bioanalytical methods validation and implementation: Best practices for chromatographic and ligand binding assays显示文摘 | C. T. Viswanathan Surendra Bansal Brian Booth Anthony J. DeStefano Mark J. Rose Jeffrey Sailstad Vinod P. Shah Jerome P. Skelly Patrick G. Swann Russell Weiner | 2007 | The AAPS Journal2007，，1： | 1 |
| 19 | Currency Crashes in Emerging Markets:An Empirical Treatment 显示文摘 | Frankel Jeffrey and Andrew Rose | 1996 | Journal of International Economics1996，，41： | 1 |
| 20 | A randomised, controlled trial of conventional versus automated weaning from mechanical ventilation using SmartCare?/PS显示文摘 | Louise Rose Jeffrey J. Presneill Linda Johnston John F. Cade | 2008 | Intensive Care Medicine2008，，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员