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| 1 | A proof of concept for neutralizing antibody-guided vaccine design against SARS-CoV-2显示文摘Mutations and transient conformational movements of the receptor binding domain(RBD)that make neutralizing epitopes momentarily unavailable present immune escape routes for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).To mitigate viral escape,we developed a cocktail of neutralizing antibodies(NAbs)targeting epitopes located on different domains of spike(S)protein.Screening of a library of monoclonal antibodies generated from peripheral blood mononuclear cells of COVID-19 convalescent patients yielded potent NAbs,targeting the N-terminal domain(NTD)and RBD domain of S,effective at n M concentrations.Remarkably,a combination of RBD-targeting NAbs and NTD-binding NAbs,FC05,enhanced the neutralization potency in cell-based assays and an animal model.Results of competitive surface plasmon resonance assays and cryo-electron microscopy(cryo-EM)structures of antigen-binding fragments bound to S unveil determinants of immunogenicity.Combinations of immunogens,identified in the NTD and RBD of S,when immunized in rabbits and macaques,elicited potent protective immune responses against SARS-CoV-2.More importantly,two immunizations of this combination of NTD and RBD immunogens provided complete protection in macaques against a SARS-CoV-2 challenge,without observable antibody-dependent enhancement of infection.These results provide a proof of concept for neutralization-based immunogen design targeting SARS-CoV-2 NTD and RBD. | Li Zhang Lei Cao Xing-Su Gao Bin-Yang Zheng Yong-Qiang Deng Jing-Xin Li Rui Feng Qian Bian Xi-Ling Guo Nan Wang Hong-Ying Qiu Lei Wang Zhen Cui Qing Ye Geng Chen Kui-Kui Lu Yin Chen Yu-Tao Chen Hong-Xing Pan Jiaping Yu Wenrong Yao Bao-Li Zhu Jianping Chen Yong Liu Cheng-Feng Qin Xiangxi Wang Feng-Cai Zhu | 2021 | National Science Review2021，8，8： | 4 |
| 2 | True-color real-time imaging and spectroscopy of carbon nanotubes on substrates using enhanced Rayleigh scattering显示文摘白光照亮的围单人赛的碳 nanotubes (SWCNT ) 应该由于散布的回声瑞利显得有颜色。然而,底层上的 SWCNT 的真彩成像没被报导,因为 SWCNT 和散布的强壮的底层的极其低的散布紧张。这里,我们证明散布的瑞利能被接口偶极子改进效果极大地提高。底层上的因而丰富多彩的 SWCNT 能被宽地 supercontinuum 激光照明直接在一台光显微镜下面想象,它便于单个 SWCNT 的高产量 chirality 赋值。这条途径,称为的瑞利成像显微镜学,没被限制为 SWCNT,但是对许多 nanomaterials 广泛地适用,它使丰富多彩的 nanoworld 能在光显微镜下面被探索。 | Wenyun Wu Jingying Yue Xiaoyang Lin Dongqi Li Fangqiang Zhu Xue Yin Jun Zhu Jiangtao Wang Jin Zhang Yuan Chen Xinhe Wang Tianyi Li Yujun He Xingcan Dai Peng Liu Yang wei Jiaping Wang Wei Zhang Yidong Huang Li Fan Lina Zhang Qunqing Li Shoushan Fan Kaili Jiang | 2015 | Nano Research2015，8，8： | 1 |
| 3 | Melatonin reduces bacterial translocation by preventing damage to theintestinal mucosa in an experimental severe acute pancreatitis rat model显示文摘 | Xuecheng Sun Yingying Shao Yin Jin Jiaping Huai Qiong Zhou Zhiming Huang Jiansheng Wu | 2013 | Experimental and Therapeutic Medicine2013，，6： | 1 |
| 4 | Interface dipole enhancement effect and enhanced Rayleigh scattering显示文摘一纳米或亚纳米的光效果包围象围单人赛的碳 nanotubes (SWCNT ) 那样的单个 nanomaterials 的压缩分子的界面的层理论上并且试验性地被学习了。当由光照亮了时,这界面的层作为一个光偶极子格子表现并且贡献在附近的原子,分子,或 nanomaterials 上提高本地地的一块即时近的地,它可以接着导致提高的瑞利散布,散布的拉曼,和荧光。这接口偶极子的理论提高了效果(IDEE ) 预言那在 nanomaterials 和界面的层的飞机之间的更小的距离,或到包围媒介的界面的层的绝缘的常数的更大的比率,将导致一个更大的领域改进因素。这预言被 SWCNT 以及在逐渐地控告的 SWCNT 散布的 situ 瑞利散布的提高的瑞利的几实现进一步试验性地验证。接口偶极子提高了散布的瑞利不仅启用真彩 nanomaterials 的即时成像,而且提供一个有效工具凝视进微妙的界面的现象。 | Wenyun Wu Jingying Yue Dongqi Li Xiaoyang Lin Fangqiang Zhu Xue Yin Jun Zhu Xingcan Dai Peng Liu Yang Wei Jiaping Wang Haitao Yang Lina Zhang Qunqing Li Shoushan Fan Kaili Jiang | 2015 | Nano Research2015，8，1： | 0 |
| 5 | Clinical relevance of distolingual roots and periodontal status in mandibular first molars: a cross-sectional study employing CBCT analysis显示文摘Objectives:Distolingual root of the permanent mandibular first molar(PMFM-DLR)has been frequently reported,which may complicate the treatment of periodontitis.This study aimed to assess the morphological features of PMFM-DLR and investigate the correlation between the morphological features of PMFM-DLR and periodontal status in patients with Eastern Chinese ethnic background.Materials and methods:A total of 836 cone beam computed tomography(CBCT)images with 1497 mandibular first molars were analyzed to observe the prevalence of PMFM-DLR at the patients and tooth levels in Eastern China.Among them,complete periodontal charts were available for 69 Chinese patients with 103 teeth.Correlation and regression analyses were used to evaluate the correlation between the morphological features of DLR,bone loss,and periodontal clinical parameters,including clinical attachment loss(CAL),probing pocket depth(PPD),gingival recession(GR),and furcation involvement(FI).Results:The patient-level prevalence and tooth-level prevalence of DLR in mandibular first molars were 29.4%and 26.3%,respectively.Multiple linear regression analysis suggested that bone loss at the lingual site and CAL were negatively affected by the angle of separation between distolingual and mesial roots in the transverse section,while they were significantly influenced by age and the angle of separation between distobuccal and mesial roots in the coronal section.Conclusions:The prevalence of PMFM-DLR in Eastern China was relatively high in our cohort.The morphological features of DLR were correlated with the periodontal status of mandibular first molars.This study provides critical information on the morphological features of DLR for improved diagnosis and treatment options of mandibular molars with DLR. | Feifei MAO Meng WANG Shuai ZHOU Yan ZHAO Jiaping HUANG Fengying YIN Haiping YANG Pei-hui DING | 2024 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2024，25，3： | 0 |
| 6 | Genome transfer for the prevention of female infertility caused by maternal gene mutation显示文摘Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertility.However,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive.Here,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female infertility.We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic transition.We next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused infertility.We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring.Moreover,those pups grow to adulthood and show normal fertility.Therefore,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation. | Dandan Bai Jin Sun Yanping Jia Jiqing Yin Yalin Zhang Yanhe Li Rui Gao Xiling Du Kunming Li Jiaming Lin Zhifen Tu Yu Wang Jiaping Pan Shanshan Liang Yi Guo Jingling Ruan Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Fengchao Wang Xiaoming Teng Wenqiang Liu Shaorong Gao | 2020 | Journal of Genetics and Genomics2020，47，6： | 0 |
| 7 | Observation of Stable Marine Boundary Layer by Shipborne Coherent Doppler Lidar and Radiosonde over Yellow Sea显示文摘Shipborne observations obtained with the coherent Doppler lidar(CDL)and radiosonde during 2014 campaign were used to study the structure of marine boundary layer in the Yellow Sea.Vertical wind profiles corrected for ship motion was used to derive higher-order statistics,showing that motion correction is required and significant for turbulence analysis.During a day with weak mesoscale activity,a complexed three-layer structure system was observed.The lowest layer showed a typical stable boundary layer structure feature.An aerosol layer with abrupt variation in wind speed and relative humidity always appeared at the middle layer,the formation of which may be due to Kelvin-Helmholz instability.The top layer encountered a dramatic change in wind direction,which may result from the warm advection from the Eurasian continent on the basis of backward trajectory analysis.Furthermore,the MABL height in stable regime was derived from potential temperature,CDL signal-to-noise ratio(SNR)and CDL vertical velocity variance,respectively.The stable boundary layer(SBL)height in SBL can be derived from the inversion layer of potential temperature profile,and the mixing height in SBL can be retrieved from the vertical velocity variance gradient method.Neither the SBL height nor the mixing height is in agreement with the height retrieved from CDL SNR gradient method because of different definition and criterion.One of the limitations of SNR gradient method for MABL retrieval is that it is easier to be affected by the lofted decoupled aerosol layer,where the retrieved result is less suitable.Finally,the higher-order vertical velocity statistics within the marine stable boundary layer were investigated and compared with the previous studies,and different turbulence mechanisms have an important effect on the statistics deviation. | Xiaochun ZHAI Songhua WU Bingyi LIU Jiaping YIN Hongwei ZHANG | 2020 | Journal of Geodesy and Geoinformation Science2020，3，4： | 0 |
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