|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Construction and activity of a novel GHRH analog, Pro-Pro-hGHRH(1-44)-Gly-Gly-Cys显示文摘AIM: To construct another growth hormone releasing hormone (GHRH) analog, Pro-Pro-hGHRH(1-44)-Gly-Gly-Cys peptide and to compare its activity with that of Pro-Pro-hGHRH(1-44)OH peptide. METHODS: The pro-pro-hGHRH(1-44)-gly-gly-cys DNA fragment was synthesized by polymerase chain reaction. The recombinant protein was expressed to high levels in Escherichia coli BL21 (DE3). The Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys peptide was purified to homogeneity by cell disruption, washing, ethanol precipitation, acid hydrolysis, and SP-Sephadex C-25 and Sephadex G-25 column chromatography. The peptide molecular mass was determined by electrospray ionization mass spectroscopy (ESI-MS). Its purity was determined by SDS-PAGE. The concentration of growth hormone (GH) stimulated by GHRH and its analogs was determined with antiserum kit against human GH. The other human hormones as hTSH, hFSH, hLH, and hPRL were determined with a paramagnetic particle chemiluminescent immunoassay kit. RESULTS: The molecular weight of Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys was 5455.4 kDa which was coincident with the theoretical calculations. All the three peptides at 5 mg/L stimulated GH release from the human fetal pituitary but only the difference between Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys group and blank group was significant (P<0.05). Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys 0.01, 0.1, and 1 mg/L and Pro-Pro-hGHRH (1-44)OH 0.1 and 1 mg/L stimulated GH release from rat pituitary in a concentration-dependent manner (P<0.05,P<0.01). At the same concentration Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys stimulated more GH release than Pro-Pro-hGHRH (1-44)OH. Pro-Pro-hGHRH (1-44)OH 0.01 mg/L and hGHRH (1-40)OH 2 mg/L did not stimulate GH release from rat pituitary (P>0.05). Pro-Pro-hGHRH (1-44)OH and Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys peptides at 5 mg/L did not stimulate hTSH, hFSH, hLH, and hPRL release from human fetal pituitary in vitro. CONCLUSION:Pro-Pro-hGHRH (1-44)-Gly-Gly-Cys had a better activity than that of Pro-Pro-hGHRH(1-44)OH. Variation at C-terminus of GHRH could modulate its GH releasing activity. | Song-shanTANG Juan-huiZHANG Ming-huaDU JieWU Jing-jingLIU | 2004 | Acta Pharmacologica Sinica2004，25，11： | 6 |
| 2 | Electrochemical hydrogenation of mixed-phase TiO2 nanotube arrays enables remarkably enhanced photoelectrochemical water splitting performance显示文摘We first report that photoelectrochemical(PEC)performance of electrochemically hydrogenated TiO_2 nanotube arrays(TNTAs)as high-efficiency photoanodes for solar water splitting could be well tuned by designing and adjusting the phase structure and composition of TNTAs.Among various TNTAs annealed at different temperature ranging from 300 to 700°C,well-crystallized single anatase(A)phase TNTAs-400 photoanode shows the best photoresponse properties and PEC performance due to the favorable crystallinity,grain size and tubular structures.After electrochemical hydrogenation(EH),anataserutile(A-R)mixed phase EH-TNTAs-600 photoanode exhibits the highest photoactivity and PEC performance for solar water splitting.Under simulated solar illumination,EH-TNTAs-600 achieves the best photoconversion efficiency of up to 1.52% and maximum H_2 generation rate of 40.4 mmol h^(-1)cm^(-2),outstripping other EH-TNTAs photoanodes.Systematic studies reveal that the signigicantly enhanced PEC performance for A-R mixed phaes EH-TNTAs-600 photoanode could be attributed to the synergy of A-R mixed phases and intentionally introduced Ti^(3+)(oxygen vacancies)which enhances the photoactivity over both UV and visible-light regions,and boosts both charge separation and transfer efficiencies.These findings provide new insight and guidelines for the construction of highly efficient TiO_2-based devices for the application of solar water splitting. | Jiaqin Liu Mengjia Dai Jian Wu Ying Hu Qi Zhang Jiewu Cui Yan Wang Hark Hoe Tan Yucheng Wu | 2018 | Science Bulletin2018，63，3： | 4 |
| 3 | Role of nucleation of bile liquid crystal in gallstone formation显示文摘AIM: To explore the role of bile liquid crystal in the process of gallbladder stone formation and to provide bases for preventing and treating cholelithiasis.METHODS: 46 guinea pigs, half males and half females,were randomly divided into control group and stone-causing group. Normal feed and stoneleading feed were used respectively to raise guinea pigs in the control group and stone-causing group. The guinea pigs were killed in three batches during the raising period. Under polarizing microscope, the pattern changes of bile liquid crystal in the gallbladder biles of the guinea pigs in the control group and stone-causing group were dynamicly observed respectively in single-blind trial.RESULTS: It was found that there were few crystals in the guinea pigs′biles of the control group, and their Malta cross was small and scattered, and existed in single form. With the increase of the feeding days, bile liquid crystals grew and Malta cross became bigger with their distribution densified, denser somewhere, but always existed in single form. While those of the stone-causing group had more bile liquid crystals, Malta cross was big and merged in strings.With the increase of the feeding days, bile liquid crystals grew in amount and strings of Malta cross increased and became bigger. The crosses in strings were arranged more and more regularly and they gradually changed into stone crystals.CONCLUSION: Formation of gallbladder stone is a process of nucleation from different substances, and the causing-stone gallbladder bile is a constantly supersaturated solution, and bile liquid crystal is a nucleation factor in the formation of gallbladder stones. The process of nucleation includes gathering, merging and phase-changing of bile liquid crystals.The process of gathering, merging of bile liquid crystal is the key to nucleation. | Hai-MingYang JieWu Jin-YiLi Min-FeiZhou | 2003 | World Journal of Gastroenterology2003，9，8： | 3 |
| 4 | Lath martensite in 1.4%C ultra-high carbon steel and its grain size effect显示文摘 | Zhu Jiewu Xu Yan Yongning Liu | 2004 | Materials Science & Engineering A2004，，1： | 2 |
| 5 | Construction of WO_(3)/Ti-doped WO_(3)bi-layer nanopore arrays with superior electrochromic and capacitive performances显示文摘Crystalline WO_(3)/Ti-doped WO_(3)bi-layer nanopore arrays were constructed by the template synthesis of a WO_(3)nanopore layer modified by a magnetron sputtering of an amorphous Ti-doped/WO_(3)layer.The obtained bi-layer nanopore array shows a remarkable electrochromic performance with large dual-band optical modulation in both visible(VIS)and near infrared(NIR)regions(optical modulation of over 70%in the wavelength range from 600 to 1600 nm)and the fast response speed(coloring for 3.4 s and bleaching for 6.6 s).In addition,the bi-layer WO_(3)/Ti-doped WO_(3)nanopore array also present superior energy-storage properties(areal capacitance of 44.0 mF cm−2 and good rate capability),better than that of titanium-free thin films.The special bifunctional characteristics of electrochromism and pseudocapacitance can be ascribed to the large specific surface area provided by the architectural design,rich ion channels in the amorphous layer as well as proper titanium doping,which bestows the bi-layer nanopore array a great potential in clean energy applications. | Yingdi Shi Shujing Liu Yong Zhang Mingjun Sun Kai Tang Jiewu Cui Xia Shu Yan Wang Jiaqin Liu Yucheng Wu | 2019 | Tungsten2019，1，3： | 2 |
| 6 | An effective amperometric biosensor based on graphene modified gold nanowire arrays for glucose detection显示文摘A novel glucose biosensor based on graphene nanosheets(GNs)modified gold nanowire arrays(AuNWAs)electrode was constructed.Highly ordered gold nanowire arrays were prepared by direct electrodeposition in anodic aluminum oxide templates.GNs were synthesized through a public route involving graphite oxidation,exfoliation,and chemical reduction.Field emission scanning electron microscope and high-resolution transmission electron microscope were employed to characterize the asprepared AuNWAs and GNs.Glucose oxidase was immobilized on the surface of GNs-AuNWAs modified electrode via a cross-linking method.The cyclic voltammetry results showed that the GNs-AuNWAs-based glucose biosensors have high catalysis activity to hydrogen peroxide(H2O2)than those modified with GNs or AuNWAs only.Furthermore,amperometric response was employed to detect glucose concentration owing to its simplicity,high selectivity,and relative low cost.Glucose biosensors based on GNs-AuNWAs showed excellent performance with high sensitivity of 40.25 lA cm-2(mmol/L)-1,low detection limit of 0.02 mmol/L,and a linear range from 0.02 to 3 mmol/L. | Jianing Hui Jiewu Cui Lingjuan Liu Guangqing Xu Yucheng Wu | 2014 | Chinese Science Bulletin2014，59，17： | 2 |
| 7 | Biomass-derived hard carbon microtubes with tunable apertures for high-performance sodium-ion batteries显示文摘Sodium-ion batteries(SIBs)are considered the most up-and-coming complements for large-scale energy storage devices due to the abundance and cheap sodium.However,due to the bigger radius,it is still a great challenge to develop anode materials with suitable space for the intercalation of sodium ions.Herein,we present hard carbon microtubes(HCTs)with tunable apertures derived from low-cost natural kapok fibers via a carbonization process for SIBs.The resulted HCTs feature with smaller surface area and shorter Na+diffusion path benefitting from their unique micro-nano structure.Most importantly,the wall thickness of HCTs could be regulated and controlled by the carbonization temperature.At a high temperature of 1,600℃,the carbonized HCTs possess the smallest wall thickness,which reduces the diffusion barrier of Na+and enhances the reversibility Na+storage.As a result,the 1600HCTs deliver a high initial Coulombic efficiency of 90%,good cycling stability(89.4%of capacity retention over 100 cycles at 100 mA·g^(−1)),and excellent rate capacity.This work not only charts a new path for preparing hard carbon materials with adequate ion channels and novel tubular micro-nano structures but also unravels the mechanism of hard carbon materials for sodium storage. | Pin Song Shiqiang Wei Jun Di Jun Du Wenjie Xu Daobin Liu Changda Wang Sicong Qiao Yuyang Cao Qilong Cui Pengjun Zhang Liaobo Ma Jiewu Cui Yan Wang Yujie Xiong | 2023 | Nano Research2023，16，4： | 1 |
| 8 | A two-parameter estimator in the negative binomial regression model显示文摘 | Jiewu HUANG Hu YANG | 2014 | Journal of Statisti- cal Computation and Simulation2014，84，1： | 1 |
| 9 | Modeling and analyzing of an enterprise relationship network in the context of social manufacturing 显示文摘 | DING Kai JIANG Pingyu LENG Jiewu | 2015 | Proceedings of the IMechE Part B:Journal of Engineering Manufacture2015，32，7： | 1 |
| 10 | Greenhouse Gas Emissions from Cotton Field under Different Irrigation Methods and Fer- tilization Regimes in Arid Northwestern China 显示文摘 | JieWu Wei Guo Jinfei Feng | 2014 | The Scien- tific World Journal2014，，： | 1 |
| 11 | Double target concept for smoking cessation显示文摘烟草使用被估计是在世界上的早熟的死亡的最大的单个原因。烟碱是在烟草产品的主要上瘾的物质。在香烟吸烟以后,烟碱快速对它的目标起作用,烟碱的醋胆素受体(nAChRs ) 它是广泛地全部散布了哺乳动物的中央神经系统并且在房间身体,树突或神经终端上被表示为多样的子类型。通过在大脑报酬电路的 nAChRs,烟碱改变 dopaminergic (DA ) 在腹的 tegmental 区域(VTA ) 和到原子 accumbens (NA ) 的从 VTA 的增加多巴胺版本的 neuronal 功能,导致烟碱报酬,忍耐和依赖。在停止吸烟以后,吸烟者们经历退却症状,包括消沉,易怒,专注或睡觉的困难,头疼,和疲劳。最近,证据被积累了揭示烟碱报酬,忍耐和依赖的分子、细胞的机制。这些调查的结果为吸停止提供药理学基础。这里,我简短总结我们烟碱报酬,忍耐和依赖的理解的最近的前进。基于这些理解,我建议一个双目标假设, nAChRs 和多巴胺版本过程在是为吸烟的二个重要目标停止。nAChRs (对抗或不敏感性) 的机能障碍是关键的废除烟碱依赖,细胞外的多巴胺的适当水平的维护消除烟碱退却症候群。因此,同时,这二上的行为指向的药应该为吸停止有需要的效果。我讨论怎么使用这个双目标概念解释最近的治疗并且为吸停止开发新候选人混合物。 | JieWU | 2010 | Acta Pharmacologica Sinica2010，31，9： | 1 |
| 12 | Integration of microfluidic injection analysis with carbon nanomaterials/gold nanowire arrays-based biosensors for glucose detection显示文摘Carbon nanotubes(CNTs) and reduced graphene oxide(r GO) nanosheets were utilized to construct glucose biosensors in combination with gold nanowire arrays(Au NWAs), and microfluidic injection analysis driven by gravity force was used to investigate the performances of as-prepared glucose biosensors. The results demonstrated glucose biosensors based on carbon nanomaterials/Au NWAs presented excellent performance at low working potential of-0.2 V versus Ag/Ag Cl(3 mol/L KCl), such as high sensitivity, good anti-interference ability and high throughput(45 h^(-1)). The glucose biosensor based on glucose oxidase(GOx)–CNT–Au NWAs showed a wide linear range from 100 to 3,000 lmol/L with a sensitivity of 4.12 l A/cm^2 mmol/L. Furthermore, the linear range and sensitivity of GOx–r GO–Au NWAs-based glucose biosensor were 50–4,000 lmol/L and 8.59 l A/(cm^2 mmol/L), respectively, which were better than those of glucose biosensor based on GOx–CNT–Au NWAs,suggesting r GO nanosheets in combination with Au NWAs being a good platform for the construction of glucose biosensors. | Yongqiang Qin Jiewu Cui Yong Zhang Yan Wang Xinyi Zhang Hongmei Zheng Xia Shu Bowen Fu Yucheng Wu | 2016 | Science Bulletin2016，61，6： | 1 |
| 13 | Earthquake ground motion simulation with frequency - dependent soil properties 显示文摘 | Jiewu Meng | 2007 | Soil Dynamics and Earthquake Engineering2007，27，3： | 1 |
| 14 | Integration of a highly ordered gold nanowires array with glucose oxidase for ultra-sensitive glucose detection显示文摘 | Jiewu Cui Samuel B. Adeloju Yucheng Wu | 2013 | Analytica Chimica Acta2013，，： | 1 |
| 15 | 显示文摘 | Tang Rui Liu Yongning Zhu Jiewu | 2004 | J Electrochem Soc2004，151，： | 1 |
| 16 | A facile and ef- ficient approach for pore-opening detection of anodic alumi- num oxide membranes 显示文摘 | Cni Jiewu Wu Yucheng Wang Yan | 2012 | Applied Su(face Science2012，258，14： | 1 |
| 17 | Enhancing the cycling stability of Na-ion batteries by bonding MoS2 on assembled carbon-based materials显示文摘Room temperature Na-ion batteries(SIBs) show great potential for use as renewable energy storage systems.However, the large-scale application of SIBs has been hindered by the lack of an ideal SIBs anode material. We synthesized MoS2 on carbonized graphene-chitosan(G-C) using the hydrothermal method. The strong interaction between the MoS2 and the G-C greatly improved the electron transport rate and maintained the structural stability of the electrode, which lead to both an excellent rate capability and long cycle stability. The G-C monolith was proven to enhance the electrical conductivity of the composites and served as a matrix for uniformly dispersing active MoS2 nanosheets(NSs), as well as being a buffer material to adapt to changes in volume during the cycle.Serving as an anode material for SIBs, the MoS2-G-C electrode showed good cycling stability(527.3mAh g-1 at100 m A g-1 after 200 cycles), excellent rate capability, and a long cycle life(439.1 m Ah g-1 at 1 A g-1 after 200 cycles). | Pin Song Jun Di Lixing Kang Manzhang Xu Bijun Tang Jun Xiong Jiewu Cui Qingsheng Zeng Jiadong Zhou Yongmin He Qundong Fu Juan Peng Shasha Guo Bo Lin Jingyu Zhang Peng Meng Zheng Liu | 2019 | Nano Materials Science2019，1，4： | 0 |
| 18 | Study on Development of the West of Shandong Province Based on Overall Regional Development Planning Pattern显示文摘The overall regional development planning pattern has been considered to be a new and effective pattern as for development of provincial economy.The article analyzed the current conditions of the west of Shandong Province and tried to find the generation mechanisms of current non-balanced economic development of Shandong Province.And then approaches to regional development were put forward according to the theory of regional development pattern as a whole. | Pang Jiewu | 2009 | Chinese Journal of Population,Resources and Environment2009，7，4： | 0 |
| 19 | Prenatal restraing stress impairs expression and function of PKCβ Ⅰ in the hippocampus of offspring rats显示文摘 | JieWu TianbaoSong LingGe | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
| 20 | The modulating effect of N coordination on single-atom catalysts researched by Pt-N_(x)-C model through both experimental study and DFT simulation显示文摘N-doped carbon-based single-atom catalysts(NC-SACs) are widely researched in various electrochemical reactions due to high metal atom utilization and catalytic activity.The catalytic activity of NC-SACs originates from the coordinating structure between single metal site(M) and the doped nitrogen(N) in carbon matrix by forming M-N_(x)-C structure(1≤x≤4).The M-N4-C structure is widely considered to be the most stable and effective catalytic site.However,there is no in-depth research for the 'x' modulation in Pt-Nx-C structure and the corresponding catalytic properties.Herein,atomically dispersed Pt on N-doped carbon(Pt-NC) with Pt-Nx-C structure(1≤x≤4),as a research model,is fabricated by a ZIF-8 template and applied to catalytic oxygen reduction.Different carbonization temperatures are used to control N loss,and then modulate the N coordination of Pt-Nx-C structure.The Pt-NC has the predictable low half-wave potential(E_(1/2)) of 0.72 V vs RHE compared to the Pt/C 20% of 0.81 V due to low Pt content.Remarkably,the Pt-NC shows a high onset potential(1.10 V vs RHE,determined for j=-0.1 mA cm^(2)) and a high current density of 5.2 mA cm^(-2),more positive and higher than that of Pt/C 20%(0.96 V) and 4.9 mA cm^(-2),respectively.As the structural characterization and DFT simulation confirmed,the reducing PtN coordination number induces low valence of Pt atoms and low free energy of oxygen reduction,which is responsible for the improved catalytic activity.Furthermore,the Pt-NC shows high mass activity(172 times higher than that of Pt/C 20%),better stability and methanol crossover resistance. | Mengmeng Fan Jiewu Cui Junjie Zhang Jingjie Wu Shuangming Chen Li Song Zixing Wang Ao Wang Robert Vajtai Yucheng Wu Pulickel MAjayan Jianchun Jiang Dongping Sun | 2021 | Journal of Materials Science & Technology2021，，32： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员