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| 1 | A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection显示文摘Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and laborious monoclonal selection process limits the production of large populations of gene-edited animals. Here, we developed a rapid and efficient method named RE-DSRNP(reporter RNA enriched dual-sg RNA/CRISPR-Cas9 ribonucleoproteins) for generating gene-edited donor cells. RE-DSRNP takes advantage of the precise and efficient editing features of dual-sg RNA and the high editing efficiency, low off-target effects, transgene-free nature, and low cytotoxic characteristics of reporter RNA enriched RNPs(CRISPR-Cas9ribonucleoproteins), thus eliminating the need for the selection of monoclonal cells and thereby greatly reducing the generation time of donor cells from 3–4 weeks to 1 week, while also reducing the extent of apoptosis and chromosomal aneuploidy of donor cells. We applied RE-DSRNP to produce cloned pigs bearing a deletion edit of the wild-type p53-induced phosphatase 1(WIP1)gene: among 32 weaned cloned pigs, 31(97%) carried WIP1 edits, and 15(47%) were homozygous for the designed fragment deletion, and no off-target event was detected. The WIP1 knockout(KO) pigs exhibited male reproductive disorders, illustrating the utility of RE-DSRNP for rapidly generating precisely edited animals for functional genomics and disease research. REDSRNP's strong editing performance in a large animal and its marked reduction in the required time for producing SCNT donor cells support its application prospects for rapidly generating populations of transgene-free cloned animals. | Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li | 2022 | Science China(Life Sciences)2022，65，8： | 9 |
| 2 | Defect and Doping Co‑Engineered Non‑Metal Nanocarbon ORR Electrocatalyst显示文摘Exploring low-cost and earth-abundant oxygen reduction reaction(ORR)electrocatalyst is essential for fuel cells and metal–air batteries.Among them,non-metal nanocarbon with multiple advantages of low cost,abundance,high conductivity,good durability,and competitive activity has attracted intense interest in recent years.The enhanced ORR activities of the nanocarbons are normally thought to originate from heteroatom(e.g.,N,B,P,or S)doping or various induced defects.However,in practice,carbon-based materials usually contain both dopants and defects.In this regard,in terms of the co-engineering of heteroatom doping and defect inducing,we present an overview of recent advances in developing non-metal carbon-based electrocatalysts for the ORR.The characteristics,ORR performance,and the related mechanism of these functionalized nanocarbons by heteroatom doping,defect inducing,and in particular their synergistic promotion effect are emphatically analyzed and discussed.Finally,the current issues and perspectives in developing carbon-based electrocatalysts from both of heteroatom doping and defect engineering are proposed.This review will be beneficial for the rational design and manufacturing of highly efficient carbon-based materials for electrocatalysis. | Jian Zhang Jingjing Zhang Feng He Yijun Chen Jiawei Zhu Deli Wang Shichun Mu Hui Ying Yang | 2021 | Nano-Micro Letters2021，13，4： | 8 |
| 3 | Core–Shell Structure and Luminescence of SrMoO_4:Eu^(3+)(10%) Phosphors显示文摘SrMoO_4:Eu^(3+)(10%) phosphors were produced via hydrothermal synthesis and co-precipitation.We systematically analyzed how the morphology and luminescence properties of the phosphors were affected by the synthesis conditions,including the p H of the precursor solution,stirring speed,and postsintering temperature.The samples synthesized at p H = 8 and 9 were spindle-like rods with a core–shell structure.When the stirring speed increased to Vs = 150 r/min,the core–shell structure disappeared.Photoluminescence measurements indicated that the Sr Mo O4:Eu3+samples under ultraviolet radiation produced strong red emission centered at 616 nm.The luminescence properties were greatly affected by the p H,stirring during hydrothermal reaction,and use of post-annealing.The related mechansim is discussed. | Yanan Zhu Ganhong Zheng Zhenxiang Dai Lingyun Zhang Jingjing Mu | 2016 | Journal of Materials Science & Technology2016，32，12： | 2 |
| 4 | Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against Fusarium crown rot显示文摘Fusarium crown rot(FCR), caused by Fusarium spp., is a chronic and severe plant disease worldwide. In the last years, the incidence and severity of FCR in China has increased to the point that it is now considered a threat to local wheat crops. In this study, for the first time, the metabolites and transcripts responsive to FCR infection in the partial resistant wheat cultivar 04 Zhong 36(04 z36) and susceptible cultivar Xinmai 26(XM) were investigated and compared at 20 and 25 days post inoculation(dpi). A total of 443 metabolites were detected, of which 102 were significantly changed because of pathogen colonization.Most of these 102 metabolites belonged to the flavonoid, phenolic acid, amino acid and derivative classes.Some metabolites, such as proline betaine, lauric acid, ribitol, and arabitol, were stably induced by Fusarium pseudograminearum(Fp) infection at two time points and may have important roles in FCR resistance. In line with the reduced seedling height of 04 z36 and XM plants, RNA-seq analysis revealed that FCR infection significantly affected the photosynthesis activities in two cultivars. Furthermore, 15 jasmonate ZIM-domain genes(JAZ) in the significantly enriched ‘regulation of jasmonic acid mediated signaling pathway’ in 04 z36 were down-regulated. The down-regulation of these JAZ genes in 04 z36 may cause a strong activation of the jasmonate signaling pathway. Based on combined data from gene expression and metabolite profiles, two metabolites, benzoxazolin-2-one(BOA) and 6-methoxy-benzoxazolin-2-one(MBOA), involved in the benzoxazinoid-biosynthesis pathway, were tested for their effects on FCR resistance. Both BOA and MBOA significantly reduced fungal growth in vitro and in vivo, and, thus, a higher content of BOA and MBOA in 04 z36 may contribute to FCR resistance. Above all, the current analysis extends our understanding of the molecular mechanisms of FCR resistance/susceptibility in wheat and will benefit further efforts for the genetic improvement of disease resistance. | Shuonan Duan Jingjing Jin Yutian Gao Changlin Jin Junyi Mu Wenchao Zhen Qixin Sun Chaojie Xie Jun Ma | 2022 | The Crop Journal2022，10，2： | 1 |
| 5 | Structures and Properties of Two New Three-Dimensional Inorganic-Organic Hybrid Compounds Based on Isopolymolybdate Clusters显示文摘包含不同 isopolymolybdate 单位的二新无机器官的混合材料,也就是[{ Ce ( pdca )( H 2 O )}{ Ce ( pdca )( H 2 O ) 5}]2{Mo8 O 26}·10H2 O ( 1 ),[ Cu ( phen )( H 2 O ) 2]2[Mo6 O 20]( 2 )( pdca=pyridine-2,6-dicarboxylic 酸, phen=1,10-phenanthroline )成功地在热水的条件下面被综合了,并且由元素的分析描绘了,红外系列,热 g 在化合物 1, Ce (III ) 离子被 pdca ligands 衔接形成铈器官的协作层,它是由经由 CeO 结合的共有原子价的 octamolybdate 簇的进一步的 pillared,导致 3D 的形成支柱分层的开框架的建筑学。超分子的结构由在 1D molybdate 之间的氢契约相互作用构造了的 3D 锁住的化合物 2 特征聚集了由[瞬间 6 O 20]4− polyoxoanions 和协调的水分子。它是显著的那 H 3[PMo12 O 40]·ΧH2 O 分解成 hexamolybdate 阴离子,它被 Cu (II ) 和 phen ligands 连接阳离子。而且,紫外力的系列和混合物 1-2 的荧光性质也详细被调查了。 | Jingjing Wang Xuena Yin Bao Mu Na Li Rudan Huang | 2016 | Chinese Journal of Chemistry2016，34，2： | 1 |
| 6 | A novel murine model to deplete hepatic stellate cells uncovers their role in amplifying liver damage in mice显示文摘 | Juan E. Puche Youngmin A. Lee Jingjing Jiao Costica Aloman Maria I. Fiel Ursula Mu?oz Thomas Kraus Tingfang Lee Hal F. Yee Scott L. Friedman | 2013 | Hepatology2013，，： | 1 |
| 7 | Stereolithography 3D printing of ceramic cores for hollow aeroengine turbine blades显示文摘Hollow superalloy or single-crystal superalloy turbine blades are the most important component of advanced aeroengines for fifth-and higher-generation fighter aircrafts [1,2,3]. The thrust-toweight ratio is largely influenced by the turbine inlet temperature,which places strict requirements on the performance of the turbine blades at high operating temperatures [4]. To reduce the operating temperature of the blades, complex internal cooling structures are required. However, the fabrication of complex cooling channels inside the blades is an unquestionably challenging task [5]. | Xiaolong An Yahang Mu Jingjing Liang Jinguo Li Yizhou Zhou Xiaofeng Sun | 2022 | Journal of Materials Science & Technology2022，，32： | 1 |
| 8 | CAFIU's Secretary-General Ni Jian Attends UN Sustainable Development Conference (Rio+20)显示文摘正Cafiu's Secretary-General Ni Jian attended UN Sustainable Development Conference (Rio +20) held in Rio de Janeiro, Brazil from June 16 to 21. This is another large-scale, high-level event in global sustainable development after the 1992 UN Environment and Development Conference. | Mu Jinling Ma Jingjing | 2012 | International Understanding2012，，2： | 0 |
| 9 | Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers显示文摘To better understand the genetic diversity and population structure of broccoli cultivars planted in China,a total of 161 representative broccoli cultivars in the past 25 years were collected and analysed based on single nucleotide polymorphism(SNP)markers.Ten pairs of primers with good polymorphism and high resolution were screened from 315 pairs of SNP primers by 3 broccoli accessions(inbred lines)with different phenotypes and maturity.The 10 pairs of SNP primers were selected,producing 78 alleles.The diversity analysis indicated that the polymorphism information content(PIC)of SNP primer ranged from 0.64 to 0.90.The observed number of alleles(Na)was 2.00,the effective number of alleles(Ne)was 1.11–2.00,the Nei’s gene diversity(H)was 0.10–0.50,and Shannon information index(I)was 0.20–0.70 using PopGene32 software.The clustering results showed that the 161 broccoli cultivars could be divided into 4 major subgroups(A,B,C and D),foreign cultivars were all assigned to subgroup A,and domestic cultivars were assigned to 3 subgroups of B,C,and D.This study indicated that some domestic cultivars and foreign cultivars were similar in genetic background,but most domestic cultivars were still different from the Japanese cultivars.When K=2,the population structure result presented that 161 broccoli cultivars could be divided into 1 simple group(2 groups)and 1 mixed group.When Q≥0.6,143(88.82%)broccoli cultivars belonged to the simple groups.In simple groups 68(42.24%)broccoli cultivars of group 1 were derived from Japan,the United States,Switzerland,the Netherlands,China-Taiwan,and China-Mainland;75(46.58%)broccoli cultivars belonged to group 2;when Q<0.6,18(11.18%)broccoli cultivars belonged to the mixed groups.This study is helpful to understand the diversity and resolution of broccoli cultivars from worldwide,which is beneficial to plant breeding and materials innovation.And meanwhile,this result is also used for construction of broccoli fingerprint serving for cultivar identification. | Jingjing Huang Yumei Liu Fengqing Han Zhiyuan Fang Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Yong Wang Jialei Ji Zhansheng Li | 2021 | Horticultural Plant Journal2021，7，5： | 0 |
| 10 | Relationship between the Level of Serum Golgi Protein 73 and the Risk of Short-term Death in Patients with ALD-ACLF显示文摘Background and Aims:As a hepatocellular carcinoma biomarker,serum Golgi protein 73(GP73)is reportedly related to inflammation.Acute-on-chronic liver failure(ACLF)is characterized by severe systemic inflammation.In this study,we aimed to explore the association between the GP73 level and short-term mortality in patients with alcohol-associated liver disease-related ACLF(ALD-ACLF).Methods:This retrospective cohort study involved 126 Chinese adults with ALD-ACLF.Baseline serum GP73 level was measured using enzymelinked immunosorbent assay.Patients were followed-up for 90 d and outcomes were assessed.Data were analyzed using multivariate Cox regression and piecewise linear regression analyses.The predictive value of GP73 and classic models for the short-term prognosis of participants were evaluated and compared using receiver operating characteristic curves.Results:The serum GP73 level was independently associated with an increased mortality risk in patients with ALD-ACLF.Compared with the lowest tertile,the highest serum GP73 level predisposed patients with ALD-ACLF to a higher mortality risk in the fully adjusted model[at 28 days:hazard ratio(HR):4.29(0.99–18.54),p=0.0511;at 90 days:HR:3.52(1.15–10.79),p=0.0276].Further analysis revealed a positive linear association.GP73 significantly improved the accuracy of the Child-Turcotte-Pugh score,model for end-stage liver disease score,and model for end-stage liver diseasesodium score in predicting short-time prognosis of patients with ALD-ACLF.Conclusions:The serum GP73 level is a significant predictor of the subsequent risk of death in patients with ALD-ACLF.GP73 improved the predictive value of classic prognostic scores. | Jingjing Tong Mingjie Yao Xiuying Mu Leijie Wang Xiajie Wen Xingran Zhai Xiang Xu Yu Wang Jing Chen Xiangwei Zhai Chongdan Guan Fengmin Lu Jinhua Hu | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 0 |
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