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| 1 | Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice显示文摘Phosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organophosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use. | Jingluan Tian Chuang Wang Qian Zhang Xiaowei He James Whelan Huixia Shou | 2012 | Journal of Integrative Plant Biology2012，54，9： | 18 |
| 2 | An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice显示文摘Male sterility(MS),characterized by functional defects in male organs or gametes,is an important agronomic trait for hybrid seed production,especially for self-pollinated crops such as rice(Oryza sativa L)(Chen and Liu,2014).Spontaneous MS mutants are rare and difficult to maintain in nature,thus limiting basicresearch and breeding.Artificial mutants are typically generated by physical,chemical,or biological mutagenesis(Wei et al,2013).Recently developed genome editing systems such as CRISPR/Cas9 allow efficient and timesaving knockout of endogenous genes at specific sites(Smith et al,2000;Moscou and Bogdanove,2009;Gasiunas et al.,2012). | Kun Ma Jingluan Han Yu Hao Zhongfang Yang Junyu Chen Yao-Guang Liu Qinlong Zhu Letian Chen | 2019 | Journal of Genetics and Genomics2019，46，5： | 6 |
| 3 | A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice显示文摘Male sterility is a prerequisite for hybrid seed production.The phytohormone gibberellin(GA)is in-volved in regulating male reproductive development,but the mechanism underlying GA homeostasis in anther development remains less understood.Here,we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1),for anther development in rice(Oryza sativa L.).Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRIPSR-associated protein 9-mediated knockout of SAW1 in rice generated similar male sterile plants.SAW1 encodes a novel nucleus-localizing CCCH-tandem zinc finger protein,and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther-specific expression.In the saw1 anther,the significantly decreased OsGA20ox3 expression resulted in lower bio-active GA content,which in turn caused the lower expression of the GA-inducible anther-regulator gene OsGAMYB.Thus,our results disclose the mechanism of the SAW1-GA20ox3-GAMYB pathway in controlling rice anther development,and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding. | Bin Wang Ruiqiu Fang Faming Chen Jingluan Han Yao-Guang Liu Letian Chen Qinlong Zhu | 2020 | Journal of Integrative Plant Biology2020，62，10： | 5 |
| 4 | Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants显示文摘Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including plants.The system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site. | Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen Jianian Tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong Xie Qinlong Zhu Yuanling Chen Xiucai Zhao Jingxin Guo Yao-Guang Liu | 2020 | Science China(Life Sciences)2020，63，6： | 4 |
| 5 | The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice显示文摘 | Yongyao Xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu Yuanling Chen Yoji Kawano Yao-Guang Liu Letian Chen | 2018 | Molecular Plant2018，11，5： | 3 |
| 6 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 7 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 8 | OsEDM2L mediates m^(6)A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation显示文摘N^(6)-methyladenosine(m^(6)A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here,we report that the N^(6)-adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE(OsEDM2 L) is essential for rice(Oryza sativa L.) anther development. The osedm2 l knockout mutant showed delayed tapetal programmed cell death(PCD) and defective pollen development. OsEDM2 L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUMDEGENERATIONRETARDATIONto regulate the expression of ETERNAL TAPETUM 1(EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2 L altered the transcriptomic m^(6)A landscape, and caused a distinct m^(6)A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2 L is indispensable for proper messenger RNA m^(6)A modification in rice anther development. | Kun Ma Jingluan Han Zixu Zhang Heying Li Yanchang Zhao Qinlong Zhu Yongyao Xie Yao‐Guang Liu Letian Chen | 2021 | Journal of Integrative Plant Biology2021，63，11： | 1 |
| 9 | Global characterization of OsPIP aquaporins reveals that the H_(2)O_(2)transporter OsPIP2;6 increases resistance to rice blast显示文摘Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress.To elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease resistance.OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant immunity.OsPIP2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)transport.Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction. | Gousi Li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen Yaling Zhang | 2024 | The Crop Journal2024，12，1： | 0 |
| 10 | OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice显示文摘In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development. | Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie | 2022 | Journal of Genetics and Genomics2022，49，5： | 0 |
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