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| 1 | A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways显示文摘Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD < 3%), precise(RSD 0.63%-2.05%), and linear(R > 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root). | ZHANG Yi-Bei DA Juan ZHANG Jing-Xian LI Shang-Rong CHEN Xin LONG Hua-Li WANG Qiu-Rong CAI Lu-Ying YAO Shuai HOU Jin-Jun WU Wan-Ying GUO De-An | 2017 | Chinese Journal of Natural Medicines2017，15，4： | 10 |
| 2 | Primary evidence for involvement of IP_(3) in heat-shock signal transduction in Arabidopsis显示文摘热吃惊(HS ) 表明的在 transducing 的肌醇 1,4,5-trisphosphate (IP (3 )) 的角色在 Arabidopsis 被检验。整个植物的 IP (3 ) 水平在 37 度 C 在 HS 的 1 min 以内增加了。在 HS 的 3 min 以后, IP (3 ) 水平到达了最大值 2.5 褶层增加。用有 AtHsp18.2 promoter-beta-glucuronidase ( GUS )的转基因的 Arabidopsis 植物熔化基因, GUS 活动的水平在 non-HS 和 HS 温度由将关入笼中的 IP ( 3 )的增加是起来调整的并且由 phospholipase C ( PLC )禁止者是下面调整的,这被发现{1-[6-(( 17beta-3-Methoxyestra-1,3,5 ( 10 ) -trien-17-yl)amino)hexyl]-2,5-pyrro lidinedione }( U-73122 )。细胞内部免费的钙离子集中([Ca (2+)](i)) 在表示 apoaequorin 的暂停有教养的 Arabidopsis 房间在 37 度 C 在 HS 期间增加了。有 U-73122 的处理阻止了增加[Ca (2+)](i) 到某程度。上面的结果在更高的植物在 HS 信号 transduction 为 IP (3 ) 的可能的参与提供了主要证据。 | Hong Tao Liu Fei Gao Shu Juan Cui Jin Long Han Da Ye Sun Ren Gang Zhou | 2006 | Cell Research2006，16，4： | 7 |
| 3 | CDH13 Genetic Polymorphisms, Adiponectin and Ischemic Stroke: a Chinese Family-based Sib-pair Study显示文摘Objective To understand the relationships between CDH13(T-cadherin) genetic polymorphisms, adiponectin levels and ischemic stroke, and possible interactions between CDH13 polymorphisms and other risk factors. Methods We recruited 342 Chinese ischemic stroke sib pairs. We genotyped rs4783244 and rs7193788 on CDH13 using time-of-flight mass spectrometry genotyping technology and measured total and high-molecular weight(HMW) adiponectin levels. We investigated associations between SNPs and ischemic stroke, and interactions between SNPs and other risk factors using multi-level mixed-effects regression model. Results In individuals without ischemic stroke, CDH13 rs4783244 was associated with total adiponectin levels(per T: Coef =-0.257, P = 0.001). CDH13 rs7193788 was associated with total adiponectin levels(per A: Coef =-0.221, P = 0.001) and HMW adiponectin levels(per A: Coef =-0.163, P = 0.003). rs7193788 was significantly associated with ischemic stroke(GA/AA vs. GG: OR = 1.55, 95% CI: 1.07 to 2.24, P = 0.020) after Bonferroni correction(α = 0.025). There was an interaction between rs7193788 and diabetes(P = 0.036). Compared to diabetes-free individuals with rs7193788 GG genotype, diabetes patients with rs7193788 GA/AA genotypes had higher risks for ischemic stroke(OR = 2.64, 95% CI: 1.58-4.40, P < 0.001). Conclusion CDH13 genetic polymorphisms are associated with adiponectin levels and ischemic stroke. An interaction is found between CDH13 SNP and diabetes for ischemic stroke. | CHEN Li SUN Ke Xin JUAN Juan FANG Kai LIU Kuo WANG Xue Yin WANG Ling YANG Chao LIU Xiao Qiang LI Jing TANG Xun WU Yi Qun QIN Xue Ying WU Tao CHEN Da Fang HU Yong Hua | 2017 | Biomedical and Environmental Sciences2017，30，1： | 4 |
| 4 | Human podocyte injury in the early course of hypertensive renal injury显示文摘BACKGROUND Hypertension is prevalent in the general population and is regarded as the second leading cause of renal damage and dysfunction,outnumbered only by diabetes.However,the mechanisms remain unclear.AIM To investigate podocyte injury induced by hypertension in the early course without massive proteinuria or renal dysfunction.METHODS The hypertension group comprised 18 patients with hypertension accompanied by microalbuminuria,diagnosed with hypertensive renal injury according to biopsy results.For a comparison of pathological changes in renal tissue,control group 1 comprised 10 healthy volunteers,and control group 2 comprised 16 patients who underwent surgery for renal trauma.RESULTS The hypertension group had significantly higher blood pressure(P=0.000)and microalbuminuria(P=0.000)compared with control group 1.In the hypertension group,urinary podocytes were detected following positive staining of podocytespecific nephrin and/or CD2-associated protein(CD2AP)in urine sediment.Podocyte foot process fusion and a significant decrease in nephrin and/or CD2AP expression in glomeruli were observed in the hypertension group compared with control group 2.This indicated that hypertension caused podocyte injury and detachment from the glomerular basement membrane,which was consistent with urinary detection of podocytes.CONCLUSION Our results suggest that podocyturia appears early in the course of hypertensive renal injury,and may be a sensitive marker for early prediction of hypertensive renal injury. | Da Sun Jiao-Jiao Wang Wei Wang Juan Wang Li-Ning Wang Li Yao Ying-Hui Sun Zi-Long Li | 2019 | World Journal of Clinical Cases2019，7，22： | 3 |
| 5 | Diameter-controlled growth of aligned single-walled carbon nanotubes on quartz using molecular nanoclusters as catalyst precursors显示文摘Molecular nanoclusters containing Fe and Mo atoms have been used as catalyst precursors for the growth of single-walled carbon nanotubes (SWNTs) on stable temperature (ST)-cut quartz substrates by chemical vapor deposition. Attribute to the uniform catalyst nanoparticles and the confinement effect of the crystalline substrates, well-aligned SWNTs with narrow diameter distribution have been synthesized. Atomic force microscopy measurements show that the mean diameter of the nanotubes obtained by thermal decomposition of ethanol at 900°C is 0.76 ± 0.16 nm, which is the smallest among all reported results for aligned SWNTs. The mean diameter of the nanotubes increases with growth temperature. In addition to using identical nanoclusters as the catalyst precursors, the avoidance of annealing treatment of catalyst precursors is also a key point for obtaining SWNTs with controlled diameters. Using these identical nanoclusters as catalyst precursors and carefully tuning the growth parameters make us closer to the ultimate goal of controlling the chirality of SWNTs. | PENG Fei LUO Da SUN Hao WANG JinYong YANG Feng LI RuoMing YANG Juan LI Yan | 2013 | Chinese Science Bulletin2013，58，4： | 3 |
| 6 | Quantitative Analysis of Eight Ginsenosides in Red Ginseng Using Ginsenoside Rg1 as Single Reference Standard显示文摘Objective:To develop a reversed-phase high-performance liquid chromatography method for the quantification of major ginsenosides in red ginseng(RG,the steamed and dried root of the cultivar of Panax ginseng C.A.Mey).Methods:A feasible method was developed in strict accordance with chromatographic properties of eight ginsenosides.Their contents could be unveiled with conventional external standard method,or as an alternative,using ginsenoside Rg1 as the single reference standard by means of seven conversion factors.Those parameters had been validated on different chromatographic columns and instruments.Results:Twenty-one batches of RG samples were determined.In addition,the chromatograms of RG and confusing species,including Panax ginseng,Panax quinquefolium,and Panax notoginseng,were apparently different.Conclusions:The method was proved to be efficient for the quality control of RG. | Juan Da Qiu-Rong Wang Yan Wang Shuai Yao Yong Huang Wen-Long Wei Jian Liang Yao Shen Gerhard Franz De-An Guo | 2021 | World Journal of Traditional Chinese Medicine2021，7，1： | 2 |
| 7 | A Risk Prediction Model for Ischemic Stroke in Southern Chinese Population:Impact of Multiple Genetic Variants and Clinical/Lifestyle Factors显示文摘With a global prevalence of 7.7 million,ischemic stroke(IS)is one of the leading causes of death and disability worldwide.In China,IS alone contributed to 69.6%of stroke events and accounted for 37.1%of the mortality/disability rate[1].IS is a complex disease that is known to be associated with various genetic variants and clinical and lifestyle risk factors[2].Genome-wide association studies(GWAS)provided evidence for the occurrence of more than 160 IS-associated single nucleotide polymorphisms(SNPs).In terms of clinical risk factors,patients with a history of chronic diseases like hypertension,dyslipidemia,and diabetes mellitus,display a higher risk of developing IS.Among the various lifestyle components,leading a sedentary lifestyle,smoking,and having an unhealthy diet are known to be associated with the risk of IS.Thus,integration of various genetic,clinical,and lifestyle variables might prove to be highly beneficial in the prediction and prevention of IS at the individual level[3]. | YE Cheng Yin LI Jin Mei WU Jing Hua LI Zheng XIAO Juan YIN Xiao Yu WANG Da Hui XU Guo Zhang YANG Lei | 2021 | Biomedical and Environmental Sciences2021，34，8： | 2 |
| 8 | Crystal and Molecular Structure of Bis-N,N’(dithiodi-2,1-phennylene)benzamide | JI Ming Juan ZHAO Zhu Liu YAN Da Yu (Graduate School of University of Science and Technology of China, Beijing, 100039) | 1999 | Chinese Journal of Structural Chemistry1999，18，2： | 2 |
| 9 | Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence显示文摘 | Juan C. Acosta Ana O’Loghlen Ana Banito Maria V. Guijarro Arnaud Augert Selina Raguz Marzia Fumagalli Marco Da Costa Celia Brown Nikolay Popov Yoshihiro Takatsu Jonathan Melamed Fabrizio d’Adda di Fagagna David Bernard Eva Hernando Jesús Gil | 2008 | Cell2008，，6： | 2 |
| 10 | HEART RATE VARIABILITY DURING HIGH-INTENSITY EXERCISE显示文摘The aim of this paper is to describe and analyse the behaviour of heart rate variability(HRV)during constant-load,high-intensity exercise using a time frequency analysis(Wavelet Transform).Eleven elite cyclists took part in the study(age:18.6±3.0 years;VO_(2max):4.88±0.61 litres·min^(-1)).Initially,all subjects performed an incremental cycloergometer test to determine load power in a constant load-test(379.55±36.02 W;89.0%).HRV declined dramatically from the start of testing(p<0.05).The behaviour of power spectral density within the LF band mirrored that of total energy,recording a significant decrease from the outset LF peaks fell rapidly thereafter,remaining stable until the end of the test.HF-VHF fell sharply in the first 20 to 30 seconds.The relative weighting(%) of HF-VHF was inverted with the onset of fatigue,[1.6%at the start,7.1(p<0.05) at the end of the first phase,and 43.1%(p<0.05) at the end of the test].HF-VHF_(peak) displayed three phases:a moderate initial increase,followed by a slight fall,thereafter increasing to the end of the test.The LF/HF-VHF ratio increased at the start,later falling progressively until the end of the first phase and remaining around minimal values until the end of the test. | SARMIENTO Samuel GARCIA-MANSO Juan Manuel MARTIN-GONZALEZ Juan Manuel VA-AMONDE Diana CALDERóN Javier DA SILVA-GRIGOLETTO Marzo Edir | 2013 | Journal of Systems Science & Complexity2013，26，1： | 2 |
| 11 | Influence of Dynamic Recrystallization on Tensile Properties of AZ31B Magnesium Alloy Sheet显示文摘 | HULI JUAN PENG YING HONG LI DA YONG | 2010 | Ma- terials and Manufacturing Processes2010，25，78： | 1 |
| 12 | Simple and robust differentiation of Ganoderma species by high performance thin-layer chromatography coupled with single quadrupole mass spectrometry QDa显示文摘In this study,a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa(HPTLC-QDa)method for robust authentication of Ganoderma lucidum,a popular and valuable herbal medicine,has been developed.This method is simple and practical,which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface.The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid(5:5:0.2,V/V)and all bands were transferred to QDa system directly in situ using 80%methanol with 0.1%formic acid as desorption solvent.The acquired HPTLC-QDa spectra showed that luminous yellow band b3,containing ganoderic acid B/G/H and ganodeneric acid B,the major active components of Ganoderma,could be found only in G.lucidum and G.lucidum(Antler-shaped),but not in G.sinense and G.applanatum.Moreover,bands b13 and b14 with m/z 475/477 and m/z 475/491/495,respectively,could be detected in G.lucidum(Antler-shaped),but not in G.lucidum,thus allowing simple and robust authentication of G.lucidum with confused species.This method is proved to be simple,practical and reproducible,which can be extended to analyze other herbal medicines. | YAO Shuai ZHANG Jian-Qing HOU Jin-Jun HU Xiao-Su WANG Ling DA Juan RAO Wei WANG Dan-Dan HUANG Yong WU Wan-Ying GUO De-An | 2021 | Chinese Journal of Natural Medicines2021，19，4： | 1 |
| 13 | Photoluminescence emissions of Ca_(1-x)WO_(4):xEu^(3+):Bridging between experiment and DFT calculations显示文摘In this work,the impact of the doping process on the photoluminescence emission of CaWO_(4) as a function of the concentration of Eu^(3+) cation(0.01 mol%,0.02 mol%,0.04 mol%,0.06 mol%,0.08 mol%,and 0.10 mol%) is discussed in detail.Ca_(1-x)WO~4:xEu^(3+) samples were successfully synthesized by a simple coprecipitation method followed by microwave irradiation.The blue shift in the absorption edge confirms the quantum confinement effect and the band gap energy covers the range from 3.91 to 4.18 eV,as the amount of Eu^(3+) cations increases.The experimental results are sustained by first-principles calculations,at the density functional theory level,to decipher the geometry and electronic properties,thereby enabling a more accurate and direct comparison between theory and experiment for the Ca_(1-x)WO_(4):xEu^(3+) structure. | Amanda Fernandes Gouveia Marcelo Assis Lara Kelly Ribeiro Aline Estefany Brandao Lima Eduardo de Oliveira Gomes Daniele Souza Yara Gobato Galvao Ieda Lucia Viana Rosa Geraldo Eduardo da Luz Jr Eva Guillamón Elson Longo Juan Andrés Miguel Angel San-Miguel | 2022 | Journal of Rare Earths2022，40，10： | 1 |
| 14 | Effects of acupuncture at acupoint neiguan( PC6 ) and intravenous Injection of Larginine on the Improvement of Acute Myocardia ischemia 显示文摘 | ZHOU Pei hua LV Juan xiu ZHU Da nian | 2006 | Joural of Acupuncture and Tuina Science2006，4，3： | 1 |
| 15 | The common anesthetic, sevoflurane, induces apoptosis in A549 lung alveolarepithelial cells显示文摘 | Gui?Hua Wei Juan Zhang Da?Qing Liao Zhuo Li Jing Yang Nan?Fu Luo Yan Gu | 2014 | Molecular Medicine Reports2014，，1： | 1 |
| 16 | Endorectal Advancement Flap显示文摘 | Nelly Mizrahi Steven D. Wexner Oded Zmora Giovanna Da Silva Jonathan Efron Eric G. Weiss Anthony M. Vernava Juan J. Nogueras | 2002 | Diseases of the Colon & Rectum2002，，12： | 1 |
| 17 | Comparative proteomics analysis of pomegranate seeds on fruit maturation period(Punica granatum L.)显示文摘Seeds play a central role in the life cycle of plants. Seed hardness in pomegranates is of economic relevance, yet scarcely studied and poorly understood in China. In this study, we compared the proteomic differences between Zhongnonghong(soft-seeded) and Sanbai(hard-seeded) pomegranates. A total of 892 protein spots from both varieties were detected on two-dimensional electrophoresis gels(2-DE); 76 spots showed greater than a 1.5-fold or less than a 0.66-fold difference(P<0.05) in Zhongnonghong compared to Sanbai, of which 24 exhibited greater than a 2-fold change. Compared with Sanbai, Zhongnonghong possessed 14 up-regulated, and 10 down-regulated proteins. We identified and annotated 5 of these by using MALDI-TOF-TOF MS: pyruvate dehydrogenase(PDH) E1-β family protein(spot 4 609); alanine aminotransferase 2-like(ALT2L); mitochondrial glycine decarboxylase complex P-protein(spot 5 803); phosphofructokinase B(Pfk B)-type family of carbohydrate kinase(spot 8 411); and putative dna K-type molecular chaperone heat shock cognate protein 70(Hsc70)(spot 9 006). Of these, 3 proteins(spots 4 609, 5 608, 5 803) were hypothesized to play a role in the formation of seed hardness. The other two proteins(spots 8 411, 9 006) were theorized to play a role in protecting the seeds from adverse stress during periods of fruit maturation. This study sets the foundation for further research on molecular mechanisms related to pomegranate seed hardness. | CAO Shang-yin NIU Juan CAO Da LI Hao-xian XUE Hui CHEN Li-na ZHANG Fu-hong ZHAO Di-guang | 2015 | Journal of Integrative Agriculture2015，14，12： | 1 |
| 18 | Sesquiterpenes from Red Alga Laurencia tristicha显示文摘Three naturally new sesquiterpenes named 10-hydroxyepiaplysin, 10-hydroxyaplysin and 10-hydroxybromoepiaplysin have been isolated from Laurencia tristicha. On the basis of the spectroscopic techniques their structures were elucidated as (3S, 3αR, 8βS)-(-)-2, 3, 3α, 8β-tetra- hydro-7-bromo-3-hydroxy-3, 3α, 6,8β-tetramethyl-1H-cyclopenta[b]benzofuran, (3R, 3αR, 8βS)- (-)-2,3,3α, 8β-tetrahydro-7-bromo-3-hydroxy-3, 3α,6,8β-tetramethyl-1H-cyclopenta[b]benzofuran and (3S, 3αR, 8βS)-(-)-2, 3,3α,8β-tetrahydro-3-hydroxy-3,3α,6,8β-tetramethyl-1H-cyclopenta[b]- benzofuran, respectively. | Jie SUN Li Jun HAN Da Yong SHI Xiao FAN Su Juan WANG Shuai LI Yong Chun YANG Jian Gong SHI | 2005 | Chinese Chemical Letters2005，16，12： | 1 |
| 19 | Soluble C D14 truncated at amino acid 152 binds lipopolysaccharide(LPS) and enables cellular response to LPS显示文摘 | Juan TS Kelley MJ Johnson DA | | 0，，： | 1 |
| 20 | Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence显示文摘 | Juan C. Acosta Ana O’Loghlen Ana Banito Maria V. Guijarro Arnaud Augert Selina Raguz Marzia Fumagalli Marco Da Costa Celia Brown Nikolay Popov Yoshihiro Takatsu Jonathan Melamed Fabrizio d’Adda di Fagagna David Bernard Eva Hernando Jesús Gil | 2008 | Cell2008，，6： | 1 |
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