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| 1 | Progress of gastric cancer etiology:N-nitrosamides in the 1990s显示文摘INTRODUCTIONStomach carcinoma is still the leading cause of cancer deathin China and the second one in the world.Its possiblecauses include:A)chemical factors such as intragastricformation of N-nitroso compounds(NOC)and high saltintake;B)biological factors such as infection | Da Jun Deng Laboratory of Cancer Etiology,Beijing Medical University School of Oncology& Beijing Institute for Cancer Research,100034,China | 2000 | World Journal of Gastroenterology2000，6，4： | 27 |
| 2 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 3 | Genome-wide Association Analyses Reveal the Genetic Basis of Stigma Exsertion in Rice显示文摘耻辱 exsertion,交配系统的米饭的一个关键决定因素,极大地在米饭贡献杂种优势的申请。尽管与耻辱 exsertion 联系的一些量的特点 loci 是印射或克隆的罚款,内在的基因建筑学仍然保持不清楚。我们用在 Oryza sativa 的 533 多样的就职描绘的 650 万 SNP 在耻辱 exsertion 和相关花的特点上执行了染色体宽的协会研究。我们识别了显著地与耻辱 exsertion 和相关特点,其三与三主要谷物尺寸基因 GS3, GW5,和 GW2 co 局部性被联系的 23 genomic loci。进一步的分析显示这三基因由控制小穗状花小穗和耻辱的尺寸和形状影响了耻辱 exsertion。GS3 和 GW5 的联合大部分定义耻辱 exsertion 和相关特点的层次。这二基因的选择在 O 的 subpopulations 之中导致了花的特点的特定的分布。sativa。低耻辱 exsertion 联合 gw5GS3 在栽培米饭变化的一半存在;因此,介绍 GW5gs3 联合进男无菌的线具有为改进混合米饭的种子生产的高潜力。 | Hao Zhou Pingbo Li WeiboXie Saddam Hussain Yibo Li Duo Xia Hu Zhao Shengyuan Sun Junxiao Chen Hong Ye Jun Hou Da Zhao Guanjun Gao Qinglu Zhang Gongwei Wang Xingming Lian Jinghua Xiao Sibin Yu Xianghua Li Yuqing He | 2017 | Molecular Plant2017，10，4： | 15 |
| 4 | 二期尿道成型术更适合于需要横断尿道板并伴有严重弯曲畸形的近段型尿道下裂显示文摘对于伴有严重阴茎弯曲的尿道下裂,横断尿道板后是选择一期还是二期手术仍存在争议。这项回顾性研究比较评价了这两种方法的优劣。本研究共收集66例伴有严重阴茎弯曲的近端型尿道下裂的患者,根据手术方式分成两组,其中32例行一期手术(Duckett),34例行二期手术。一期和二期手术组的中位年龄分别为7.5岁和11.0岁,中位随访时间分别为28.5个月和35个月。所有患者术后尿道外口均位于龟头顶端。随访期间无患者出现阴茎弯曲复发,患者及其家属对阴茎的长度和外观均满意。术后两组各有8例患者出现并发症,两组间并发症率无统计学差异。一期手术尿道狭窄的远期并发症率高j(18.75%1,JO%)。在一期手术组中,青春期前患者的并发症率显著低于青春期后患者(10.52%w46.15%3。中期随访结果提示,横断尿道板可有效矫正近端型尿道下裂合并的重度阴茎弯曲。考虑到一期手术组中较高的尿道狭窄发生率,本文认为分期手术更适合伴有严重阴茎弯曲需要横断尿道板的近端型尿道下裂。 | Da-Chao Zheng Hai-Jun Yao Zhi-Kang Cai Jun Da Qi Chen Yan-Bo Chen Ke Zhang Ming-Xi Xu Mu-Jun Lu Zhong Wang | 2015 | Asian Journal of Andrology2015，17，1： | 13 |
| 5 | Relationship of particle stimulated nucleation,recrystallization and mechanical properties responding to Fe and Si contents in hot-extruded 7055 aluminum alloys显示文摘The variations of coarse intermetallic particles in hot-extruded 7055 aluminum alloys with 0.041 wt%Fe and 0.024 wt%Si increasing to 0.272 wt%Fe and 0.134 wt%Si were investigated.The particle stimulated nucleation(PSN)behaviors for different kind of coarse particles were detailly analyzed by EBSD.Moreover,the effect of PSN responding to Fe and Si contents on recrystallization and tensile properties of 7055 alloys was evaluated.With increasing Fe and Si contents,the size and number density of coarseη/S particles are reduced,while the number densities of coarse Al7Cu2 Fe and Mg2Si particles are both increased and the coarse Al7Cu2 Fe particles transform from rod-like to irregular.More PSN recrystallized grains with predominant orientations deviated from the extruded fiber textures are stimulated by the irregular Al7Cu2 Fe and Mg2Si particles,because a higher degree of local non-uniform deformation is produced.The rod-like Al7Cu2 Fe particles cause the greatest degree of local non-uniform deformation owing to the largest aspect ratio,but the shape also restricts the area of particle deformation zone(PDZ)resulting in fewer PSN recrystallized grains.The irregularη/S particles give rise to the lowest degree of local non-uniform deformation and fewest PSN recrystallized grains with the major orientations close to the extruded fiber textures.Consequently,despite the number and size of coarseη/S particles are reduced,the proportion of high angle grain boundaries(HAGBs)is increased and the extruded fiber textures are weakened with Fe and Si contents increasing,because of the increased Al7Cu2 Fe and Mg2Si particles.The strength is slightly declined by the weakened<111>//ED(extrusion direction)fiber texture,while the elongation is reduced for a larger number of coarse particles and more HAGBs with higher Fe and Si contents. | Huan She Da Shu Anping Dong Jun Wang Baode Sun Hongchang Lai | 2019 | Journal of Materials Science & Technology2019，35，11： | 10 |
| 6 | Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?显示文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. | DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei | 2020 | Biomedical and Environmental Sciences2020，33，12： | 9 |
| 7 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 8 | Ectopic expression of soybean GmKNT1 in Arabidopsis results in altered leaf morphology and flower identity显示文摘Plant morphology is specified by leaves and flowers, and the shoot apical meristem (SAM) defines the architecture of plant leaves and flowers. Here, we reported the characterization of a soybean KNOX gene GmKNT1, which was highly homologous to Arabidopsis STM. The GmKNT1 was strongly expressed in roots, flowers and developing seeds. Its expression could be induced by IAA, ABA and JA, but inhibited by GA or cytokinin. Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region, SAM of young leaves, sepal and carpel, not in seed and mature leaves. Scanning electron micros-copy (SEM) disclosed multiple changes in morphology of the epidermal cells and stigma. The transgenic Arabidopsis plants overexpress-ing the GmKNT1 showed small and lobed leaves, shortened internodes and small clustered inflorescence. The lobed leaves might result from the function of the meristems located at the boundary of the leaf. Compared with wild type plants, transgenic plants had higher ex-pression of the SAM-related genes including the CUP, WUS, CUC1, KNAT2 and KNAT6. These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression. | Jun Liu Da Ha Zongming Xie Chunmei Wang Huiwen Wang Wanke Zhang Jinsong Zhang Shouyi Chen | 2008 | Journal of Genetics and Genomics2008，35，7： | 8 |
| 9 | Can ultrasound predict the severity of acute pancreatitis early by observing acute fluid collection?显示文摘INTRODUCTIONThe spectrum of acute pancreatitis (AP) ranges from a mild spontaneously resolved disorder to severe disease with mortality up to 20%-48.4%[1-3]. sAP is defined as the AP with organ failure and /or local complications which developed form acute fluid collection (AFC) including necrosis ,abscess , pseudocyst formation into or around the pancreas [4]. | Yan Luo1 Chao Xin Yuan1 Yu Lan Peng1 Pei Lin Wei1 Zhao Da Zhang2 Jun Ming Jiang3 Lin Dai1 Yun Kai Hu1 1Ultrasound Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China2Surgery Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China3Tranditional Chinese Medicine, Department of First Affiliated Hospital of West China University of Medical Science, Chengdu 610041, Sichuan Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 8 |
| 10 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 11 | H2 strain attenuated live hepatitis A vaccines:protective efficacy in a hepatitis A outbreak显示文摘AIM To investigate the protective efficacy ofH2 strain attenuated live hepatitis A vaccines(H2-strain vaccines)in hepatitis A(HA)outbreaks.METHODS With the permission of theirparents,5551 pre-school and grade 1-3 primaryschool children were inoculated with 1 dose(106.5TCID50)of H2-strain vaccines in anonrandomized,controlled trial conducted inFucheng County,Hebei Province in May 1997.Another 6485 children in the same grades andcompatible in gender and age were enrolled ascontrols.Epidemiological and serological surveywas conducted to evaluate the protectiveefficacy of the vaccines.ELISA was used todetect serum IgM anti-HAV.RESULTS HA outbreak started in early May1998,peaked in the middle of the same month,and lasted about 80 days.Overall 302 HA caseswere found,192(53.58%)were 5-9 years old.One vaccinee and 25 control cases were found tohave hepatitis A,which account for 0.28%(1/356)and 5.92%(25/422)of all vaccinees andcontrols in the 14 villages,respectively.The protective efficacy of vaccines was 95.27%(95% Cl:85.83%-104.72%).In subjects testedfor anti-HAV IgM from 13 villages,1(0.40%)overt and 11(4.06%)asymptomatic HAV caseswere found in 271 vaccinees,but 21(6.69%)ofovert and asymptomatic ones were found in 314controls.CONCLUSION H2 strain vaccines were excellent in preventing overt hepatitis A, but not so effective in preventing asymptomatic hepatitis A virus infection. A booster dose might be needed to get permanent reliable immunity. | Yu Liang Zhao Zong Da Meng Zhi Yi Xu Jun Jie Guo Shao Ai Chai Cheng Gang Duo Xuan Yi Wang Jin Feng Yao Hong Bin Liu Shun Xiang Qi Hui Bin Zhu | 2000 | World Journal of Gastroenterology2000，6，6： | 7 |
| 12 | Long Campaign of BF with Overheating-Free Cooling Stave显示文摘A basic idea of permanent lining in designing long campaign of BF was put forward. BF designer should follow a chain of thinking procedure: Cooling water-Cooling system-Refractory lining. The temperature field of BF wall and cooling stave was calculated using heat transfer model and the results were proved by a 1 000-1 200 ℃ test rig. The overheating-free cooling stave with different parameters was designed, and such a long campaign of BF (15 years) was going to put into construction. | CHENG Su sen YANG Tian jun CANG Da qiang | 2003 | Journal of Iron and Steel Research(International)2003，10，3： | 7 |
| 13 | Total synthesis of bis-(2,3-dibromo-4,5-dihydroxyphenyl)-methane as potent PTP1B inhibitor显示文摘蛋白质酷氨酸磷酸酶 1B (PTP1B ) 作为一个否定管理者起一个重要作用并且被证明了是为类型 2 糖尿病 mellitus 的处理的一个有效目标。二度 --(2,3-dibromo-4,5-dihydroxyphenyl ) 甲烷 7 首先对从红水藻 Rhodomela confervoides 被孤立的 PTP1B 与重要抑制作为自然 bromophenol 被报导。由它的令人震惊的活动好奇(IC50 = 2.4 渭 m ol/L ) ,化合物 7 与 24% 的全面收益被综合并且与自然化合物相比为它的 PTP1B 禁止的活动评估了。 | Jing Li Shu Ju Guo Hua Su Li Jun Han Da Yong Shi | 2008 | Chinese Chemical Letters2008，19，11： | 6 |
| 14 | Genetic Analysis of ele Mutants and Comparative Mapping of ele1 Locus in the Control of Organ Internal Asymmetry in Garden Pea显示文摘Previous study has shown that during zygomorphic development in garden pea(Pisum sativum L.),the organ internal(IN) asymmetry of lateral and ventral petals was regulated by a genetic locus,SYMMETRIC PETAL 1(SYP1),while the dorsoventral(DV) asymmetry was determined by two CYC-like TCP genes or the PsCYC genes,KEELED WINGS(K) and LOBED STANDARD 1(LST1).In this study,two novel loci,ELEPHANT EAR-LIKE LEAF 1(ELE1) and ELE2 were characterized.These mutants exhibit a similar defect of IN asymmetry as syp1 in lateral and ventral petals,but also display pleiotropic effects of enlarged organ size.Genetic analysis showed that ELE1 and ELE2 were involved in same genetic pathway and the enlarged size of petals but not compound leaves in ele2 was suppressed by introducing k and lst1,indicating that the enlargement of dorsal petal in ele2 requires the activities of K and LST1.An experimental framework of comparative genomic mapping approach was set up to map and clone LjELE1 locus in Lotus japonicus.Cloning the ELE1 gene will shed light on the underlying molecular mechanism during zygomorphic development and further provide the molecular basis for genetic improvement on legume crops. | Xin Li Li-Li Zhuang Mike Ambrose M. Phil. Catherine Rameau Xiao-He Hu Jun Yana Da Luo | 2010 | Journal of Integrative Plant Biology2010，52，6： | 6 |
| 15 | Multiple Components are Integrated to Determine Leaf Complexity in Lotus japonicus显示文摘Transcription factors and phytohormones have been reported to play crucial roles to regulate leaf complexity among plant species. Using the compound-leafed species Lotus japonicus, a model legume plant with five visible leaflets, we characterized four independent mutants with reduced leaf complexity, proliferating floral meristem (pfm), proliferating floral organ-2 (pfo-2), fused leaflets1 (ful1) and umbrella leaflets (uml), which were further identified as loss-of-function mutants of Arabidopsis orthologs LEAFY (LFY ), UNUSUAL FLORAL ORGANS (UFO), CUP-SHAPED COTYLEDON 2 (CUC2) and PIN-FORMED 1 (PIN1), respectively. Comparing the leaf development of wild-type and mutants by a scanning electron microscopy approach, leaflet initiation and/or dissection were found to be affected in these mutants. Expression and phenotype analysis indicated that PFM/LjLFY and PFO/LjUFO determined the basipetal leaflet initiation manner in L. japonicus. Genetic analysis of ful1 and uml mutants and their double mutants revealed that the CUC2-like gene and auxin pathway also participated in leaflet dissection in L. japonicus, and their functions might influence cytokinin biogenesis directly or indirectly. Our results here suggest that multiple genes were interplayed and played conserved functions in controlling leaf complexity during compound leaf development in L. japonicus. | Zhenhua Wang Jianghua Chen Lin Weng Xin Li Xianglin Cao Xiaohe Hu Da Luo Jun Yang | 2013 | Journal of Integrative Plant Biology2013，55，5： | 6 |
| 16 | Advances in lithium niobate photonics:development status and perspectives显示文摘Lithium niobate(LN)has experienced significant developments during past decades due to its versatile properties,especially its large electro-optic(EO)coefficient.For example,bulk LN-based modulators with high speeds and a superior linearity are widely used in typical fiber-optic communication systems.However,with everincreasing demands for signal transmission capacity,the high power and large size of bulk LN-based devices pose great challenges,especially when one of its counterparts,integrated silicon photonics,has experienced dramatic developments in recent decades.Not long ago,high-quality thin-film LN on insulator(LNOI)became commercially available,which has paved the way for integrated LN photonics and opened a hot research area of LN photonics devices.LNOI allows a large refractive index contrast,thus light can be confined within a more compact structure.Together with other properties of LN,such as nonlinear/acousto-optic/pyroelectric effects,various kinds of high-performance integrated LN devices can be demonstrated.A comprehensive summary of advances in LN photonics is provided.As LN photonics has experienced several decades of development,our review includes some of the typical bulk LN devices as well as recently developed thin film LN devices.In this way,readers may be inspired by a complete picture of the evolution of this technology.We first introduce the basic material properties of LN and several key processing technologies for fabricating photonics devices.After that,various kinds of functional devices based on different effects are summarized.Finally,we give a short summary and perspective of LN photonics.We hope this review can give readers more insight into recent advances in LN photonics and contribute to the further development of LN related research. | Guanyu Chen Nanxi Li Jun Da Ng Hong-Lin Lin Yanyan Zhou Yuan Hsing Fu Lennon Yao Ting Lee Yu Yu Ai-Qun Liu Aaron J.Danner | 2022 | Advanced Photonics2022，4，3： | 6 |
| 17 | NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice显示文摘在 monocot 米饭种类 Oryza sativa L. ,最惹人注目的词法过程之一在繁殖开发期间是有上面的 internodes (合众国际社) 的顺序的延伸的圆锥花序开发的同时发生。阐明内在的分子的机制,我们克隆米饭基因颈叶 1 (NL1 ) ,什么时候变异,它在 flowering 时间,有簇叶丛生的苞的更小的圆锥花序和反常合众国际社延伸模式导致延期。NL1 基因与一个单个锌手指领域,和它的抄本编码一个 GATA 类型抄写因素在苞 primordia 主要被检测,它通常在野类型的植物堕落。在转基因的植物的 NL1 的 Overexpression 经常产生严重生长延迟,不太植物的 phytomers 和更小的叶子,建议 NL1 起在器官区别的一个重要作用。PLASTOCHRON1 (PLA1 ) 的新奇变异的等位基因,知道在调整叶开始起一个关键作用的基因,在这研究被识别。基因分析表明了在 nl1 和 pla1 之间的一个相互作用,与 PLA1 在上游的代理的 NL1。表示水平和 PLA1 的空间模式被发现在 nl1 被改变变异。而且, flowering, Hd3a 和 OsMADS1 的二个管理者的表示,也在 nl1 异种被影响。根据这些调查结果,我们建议 NL1 是通过在米饭在繁殖开发期间调整 PLA1 和另外的规章的基因的表示调制并且协调 organogenesis 的一个内在的因素。 | Liping Wang Hengfu Yin Qian Qian Jun Yang Chaofeng Huang Xiaohe Hu Da Luo | 2009 | Cell Research2009，19，5： | 6 |
| 18 | Functional Diversity of CYCLOIDEA-like TCP Genes in the Control of Zygomorphic Flower Development in Lotus japonicus显示文摘CYCLOIDEA (CYC)-like TCP genes play key roles in dorsoventral differentiation of zygomorphic flowers in Papilionoideae legumes. In this study, we analyzed the kew mutants whose flowers lost lateral identity, and investigated the diverse functions of three LjCYC genes during zygomorphic flower development in the model legume Lotus japonicus. We showed that kew1 and kew3 are allelic mutants of LjCYC3, a CYC-like TCP gene. Through transgenic experiments, it was shown that LjCYC1 possesses dorsal activity similar to LjCYC2, and that LjCYC3 alone is sufficient to confer lateral activity, and an epistatic effect between dorsal and lateral activities was identified. Sequence analysis revealed a striking alteration at the 3 end of the LjCYC3 open reading frame (ORF) in comparison with those of LjCYC1 and LjCYC2 ORFs. Furthermore, it was found that LjCYC proteins could interact with each other and possess different activities by means of a transcriptional activity assay. Our data demonstrate that the sequence variation and the subsequent alteration of protein property play important roles in the functional diversity of different LjCYC genes in controlling zygomorphic flower development in Lotus japonicus. | Shilei Xu Yonghai Luo Zhigang Cai Xiangling Cao Xiaohe Hu Jun Yang Da Luo | 2013 | Journal of Integrative Plant Biology2013，55，3： | 6 |
| 19 | Hwangryunhaedoktang exerts anti-inflammation on LPS-induced NO production by suppressing MAPK and NF-κB activation in RAW264.7 macrophages显示文摘OBJECTIVE: This study aimed to evaluate whether Hwangryunhaedoktang(HHT), a herbal compound, has an inhibitory effect on lipopolysaccharide(LPS)-induced inflammation in RAW264.7 macrophages.METHODS: The effects of HHT were evaluated by confirming nitric oxide(NO) production and expression of inducible NO synthase(iN OS) and mitogen-activated protein kinases(MAPKs) in LPS-stimulated RAW264.7 macrophages via the Griess assay, Western blotting, and real-time reverse transcription quantitative polymerase chain reaction. Western blot analyses and luciferase assays were used to evaluate whether HHT has an effect on the phosphorylation and translocation of nuclear factor-κB(NF-κB). The secretion and expression of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were determined via enzyme-linked immunosorbent assay and Western blot analyses.RESULTS: HHT suppressed LPS-induced NO production and expression of iN OS in a dose-dependent manner. Additionally, MAPKs activation was also attenuated via inhibition of phosphorylation of extracellular signal-regulated kinases 1/2, c-Jun N-terminal kinase and p38 which were related to inflammatory pathway. Furthermore, HHT also effectively attenuated NF-κB activation and its translocation to the nucleus, a process that is closely linked to inflammation. LPS normally induced the expression of inflammatory cytokines such as TNF-α and IL-6, but the secretion and expression of TNF-α and IL-6 were significantly attenuated by pretreating the cells with HHT.CONCLUSION: HHT suppressed LPS-induced NO production by blocking the activation of NF-κB and MAPK signaling pathways in RAW264.7 macrophages. Furthermore, HHT may have an anti-inflammatory effect by suppressing the LPS-induced secretion of TNF-α and IL-6. Therefore, the traditional herbal formula HHT might be a useful potential therapeutic agent for inflammation. | Byung Hyuk Han Yun Jung Lee Jung Joo Yoon Eun Sik Choi Seung Namgung Xian Jun Jin Da Hye Jeong Dae Gill Kang Ho Sub Lee | 2017 | Journal of Integrative Medicine2017，15，4： | 5 |
| 20 | Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress-autophagy axis and improves myocardial reconstruction after acute myocardial infarction显示文摘During acute myocardial infarction,endoplasm ic reticulum(ER)stress-induced autophagy and apoptosis have been shown as im portant pathogeneses of myocardial reconstruction.Im portantly,hydrogen sulfide(H2S),as a third endogenous gas signaling molecule,exerts strong cytoprotective effect on anti-ER stress,autophagy regulation and antiapoptosis.Here,we showed that H2 S treatm ent inhibits apoptosis by regulating ER stress-autophagy axis and im proves myocardial reconstruction after acute myocardial infarction.We found that H2S intervention im proved left ventricle function,reduced glycogen deposition in myocardial tissue mesenchyme,and inhibited apoptosis.Moreover,the expressions of fibrosis indicators(Col3a1 and Col1a2),ER stress-related proteins(CHOP and BIP/ERP78),autophagy-related proteins(Beclin and ATG5),apoptosis protein(Bax),as well as fibrosis protein Col4a3bp were all decreased after treatm ent w ith H2 S.H2 S adm inistration also m aintained MMP/TIMP balance.Mechanistically,H2S activated the PI3K/AKT signaling pathway.In addition,H2S treatm ent also reduced the expressions of ER stress-related proteins,autophagy-related proteins,and apoptins in in wtro experiments.Interestingly,activation of ER stress-autophagy axis could reverse the inhibitory effect of H2S on myocardial apoptosis.Altogether,these results suggested that exogenous H2S suppresses myocardial apoptosis by blocking ER stress-autophagy axis,which in turn reverses cardiac rem odeling after myocardial infarction. | Yaling Li Maojun Liu Jiali Yi Xiong Song Xia Zheng Da Liu Sen Wang Chun Chu Jun Yang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 5 |
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