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| 1 | The influence of the Madden-Julian Oscillation activity anomalies on Yunnan's extreme drought of 2009-2010显示文摘Yunnan Province of China suffered a record-breaking drought that persisted from autumn 2009 into spring 2010. The present study examined the physical causes of this extreme drought event in terms of persistent anomalies of intraseasonal oscillation in the tropical atmosphere (the Madden-Julian Oscillation hereafter the MJO). The results show that the occurrence of severe drought in Yunnan was caused directly by deficient rainfall lasting from the summer of 2009 to the spring of 2010. Further exploration reveals a persistent positive variation of MJO index 1 from June to October. Accordingly, the convective activity over the Bay of Bengal (BOB) weakened continuously, and then an anomalous descending airflow was induced over the tropical Indian Ocean, resulted in the anomalous weakening of vertical Asian monsoon circulation in South Asia. Consequently, the transport of water vapor from the tropical Indian Ocean to Yunnan decreased abnormally, leading to persistent below-normal rainfall over Yunnan from summer to autumn in 2009. As a result, a severe drought began to appear in autumn. In the winter of 2009-2010, MJO index 1 remained persistently positive, indicating the continuous weakening of convective activity over the BOB. The atmospheric circulation associated with the persistent positive anomalies in the MJO also demonstrated anomalous patterns. Specifically, there was an anomalous high-pressure ridge stretching from South Asia through the Tibetan Plateau and into the western part of southwestern China. This indicates that the atmospheric circulation over Yunnan was dominated by vertical descending airflow in the high-pressure ridge. Simultaneously, the India-Burma trough was weakened, which resulted in unfavorable conditions for the transport of water vapor from the BOB to Yunnan, causing the observed persistent deficient precipitation in winter and the subsequently intensified drought. Therefore, the persistent anomalies in MJO activity in the tropical atmosphere played an important role in the occurrence of the extreme drought event in Yunnan in 2009-2010. | LU JunMei JU JianHua REN JuZhang GAN WeiWei | 2012 | Science China Earth Sciences2012，55，1： | 25 |
| 2 | Gene mutations and clinical phenotypes in Chinese children with Blau syndrome显示文摘The mutations of CARD15 gene and clinical features of Chinese patients with Blau syndrome were analyzed. We identified10 missense mutations, out of which five were new: R334 L, E383 D, R471 C, C495 R and D512 F. The rest of them, R334 W,R334Q, G481 D, M513 T and R587 C, have been reported previously. Among all the mutations, R334 W, R334 Q and C495 R had the highest frequency. Blau syndrome was found at early age after birth. It began with lepidic rash and symmetric polyarthritis and was phenotypically characterized by typical rash, arthritis, iridocyclitis and arteritis. Cardiac involvement was also found in Blau syndrome. In addition to nerve deafness, renal involvement, osteochondroma and central nervous system involvement were also found in our patients. Therefore, Chinese children with Blau syndrome have unique gene mutations and complicated clinical phenotypes. Pathologic examination and CARD15 mutation testing should be considered for diagnosis as early as possible for suspected patients. | Caifeng Li Junmei Zhang Shipeng Li Tongxin Han Weiying Kuang Yifang Zhou Jianghong Deng Xiaohua Tan | 2017 | Science China(Life Sciences)2017，60，7： | 13 |
| 3 | Gene mutations and clinical phenotypes in 15 Chinese children with cryopyrin-associated periodic syndrome(CAPS)显示文摘The aim of our study is to explore the features of clinical manifestations and genetic mutations in Chinese CAPS patients. Fifteen confirmed patients with CAPS were enrolled. The onset time ranges from 2 days after birth to 6 years and 1 month. Recurrent urticaria rash(93.3%) with fever(100%) were two dominant characteristics of these patients that were presented as either acute or chronic process. Systemic involvements were found in all patients except for one with only rash and fever. The top three symptoms were fever(100%), rash(93.3%) and myalgia(76%). Other clinical manifestations include arthritis(11 cases), lung involvement(seven cases), optical dysfunction(seven cases), nerve deafness(six cases), nervous system involvement(five cases), hepatomegaly, splenomegaly and lymphadenectasis(five cases). Also, four patients had heart involvement and one patient suffered kidney involvement. The laboratory inflammation index such as leukocyte counts, platelet counts, erythrocyte sedimentation rate(ESR), C-reactive protein(CRP), serum amyloid A(SAA) and fibrinogen(FIB) increased significantly at initial stage, but decreased after therapy. As for gene mutation detection, Twelve out of 15 patients were confirmed with mutation in NLRP3, including 11 mutant site: c1789 A>G, c.1703 T>A, c.913 G>A, c.1710 G>C, c.1057 G >T, c.2335 C>T, c.932 T>C,c.296 G>C, c.663 C>T, t.1702 T>A, 299 G>A. Mutation sites c.1703 T>A, c.2335 C>T, c.296 G>C, c.663 C>T, and 299 G>A were newly identified. The association between gene mutation and clinical manifestation shows that D305 N was highly associated with severe organs involvements, and therefore, the time of therapy and regimen were critical for the prognosis of disease. As the largest cohort study of Chinese CAPS patients, we confirmed that all patients presented typical clinical manifestations, identified five new mutation sites on NLRP3 and analyzed the correlation between the genotypes and phenotypes. We also raise concerns for one case with serious conditions that only had two nonsense mutations(c.663 C>T and t.1702 T>A) detected. | Caifeng Li Xiaohua Tan Junmei Zhang Shipeng Li Wenxiu Mo Tongxin Han Weiying Kuang Yifang Zhou Jianghong Deng | 2017 | Science China(Life Sciences)2017，60，12： | 9 |
| 4 | Reviews of Metallurgical Technology to Recovery Platinum Group Metals from Secondary Resource in China显示文摘China is extremely poor in mineral resources of Platinum Group Metals (PGMs), productive output of PGMs from mineral resource is 2.5 tons per year. At the same time, China is the biggest PGMs consumption country in the world, the mineral resource of PGMs is critical shortage, it shows the importance of recycling the secondary resource of PGMs. Sino-Platinum Metals Resource (Yimen) Co., Ltd. is the leader in recycling of PGMs from secondary resource, and has made outstanding contributions to China PGMs secondary resources recycling. This article elucidates the current situation of secondary resources recovery and development of metallurgical technology for PGMs. | GUO Junmei HE Xiaotang WANG Huan WU Xilong ZHAO Yu LI Yong LI Kun HAN Shouli | 2012 | 贵金属2012，33，A01： | 9 |
| 5 | Strong ozone production at a rural site in the North China Plain: Mixed effects of urban plumes and biogenic emissions显示文摘Regional ozone(O_3) pollution has drawn increasing attention in China over the recent decade, but the contributions from urban pollution and biogenic emissions have not been clearly elucidated. To better understand the formation of the regional O_3 problem in the North China Plain(NCP), intensive field measurements of O_3 and related parameters were conducted at a rural site downwind of Ji'nan, the capital city of Shandong province, in the summer of 2013. Markedly severe O_3 pollution was recorded, with the O_3 mixing ratios exceeding the Chinese national ambient air quality standard on 28 days(a frequency of 78%) and with a maximum hourly value of 198 ppbv. Extensive regional transport of wellprocessed urban plumes to the site was identified. An observation-constrained chemical box model was deployed to evaluate in situ photochemical O_3 production on two episodes.The results show that the in situ formation accounted for approximately 46% of the observed O_3 accumulation, while the remainder(~54%) was contributed by regional transport of the O_3-laden urban plumes. The in situ ozone production was in a mixed controlled regime that reducing either NOx or VOCs would lead to a reduction of ozone formation. Biogenic VOCs played an important role in the local ozone formation. This study demonstrates the significant mixed effects of both anthropogenic pollution from urban zones and biogenic emission in rural areas on the regional O_3 pollution in the NCP region,and may have general applicability in facilitating the understanding of the formation of secondary pollution over China. | Ruihan Zong Xue Yang Liang Wen Caihong Xu Yanhong Zhu Tianshu Chen Lan Yao Liwei Wang Junmei Zhang Lingxiao Yang Xinfeng Wang Min Shao Tong Zhu Likun Xue Wenxing Wang | 2018 | Journal of Environmental Sciences2018，30，9： | 8 |
| 6 | Copper-Bismuth Bimetallic Microspheres for Selective Electrocatalytic Reduction of CO2 to Formate显示文摘Summary of main observation and conclusion Electrocatalytic carbon dioxide reduction holds great promise for reducing the atmospheric CO2 level and alleviating the energy crisis. High-performance electrocatalysts are often required in order to lower the high overpotential and expedite the sluggish reaction kinetics of CO2 electroreduction. Copper is a promising candidate metal. However, it usually suffers from the issues of poor stability and low product selectivity. In this work, bimetallic Cu-Bi is obtained by reducing the microspherical copper bismuthate (CuBi2O4) for selectively catalyzing the CO2 reduction to formate (HCOO-). The bimetallic Cu-Bi electrocatalyst exhibits high activity and selectivity with the Faradic efficiency over 90% in a wide potential window. A maximum Faradaic efficiency of ~95% is obtained at -0.93 V versus reversible hydrogen electrode. Furthermore, the catalyst shows high stability over 6 h with Faradaic efficiency of -95%. This study provides an important clue in designing new functional materials for CO2 electroreduction with high activity and selectivity. | Lin Jia Hui Yang Jun Deng Junmei Chen Yuan Zhou Pan Ding Leigang Li Na Han Yanguang Li | 2019 | Chinese Journal of Chemistry2019，37，5： | 8 |
| 7 | Arsenic trioxide and microRNA-204 display contrary effects on regulating adipogenic and osteogenic differentiation of mesenchymal stem cells in aplastic anemia显示文摘我们的以前的研究证明了砷三氧化物(ATO ) 在与无形的贫血症(AA ) 对待病人有临床的功效。然而,机制尚待被阐明。骨头髓的重要部件造血的微型环境,骨头髓间充质的干细胞(BMSC ) ,经常在 AA 病人被改变。在这研究, AA BMSC 是容易的被导致进 adipocytes 而非造骨细胞,这被发现。ATO 治疗能部分至少恢复 AA BMSC 的区别不平衡。我们进一步在 AA BMSC 区别作为一个关键管理者识别了 miR-204。酶记者试金证明 miR-204 能直接绑在 Runx2 mRNA 的 3-untranslated 区域,调整成骨的一个关键抄写因素。而且, adipogenic 区别被支持, osteogenic 区别在 miR-204 被禁止过去表示的房间而成骨被提高, adipocyte 形成在房间被禁止,那失去了 miR-204 功能,它建议了它的内长的功能。一起我们证明 ATO 能禁止 adipogenic 区别,但是在 AA BMSC 支持 osteogenic 区别,在 AA 病人为 ATO 临床的功效提供可能的解释。MiR-204 戏在调整 BMSC 区别的一个关键角色,和下面调整的 miR-204 表示可能是新奇策略对待 AA。 | Junmei Zhao Chao Wang Yongping Song Baijun Fang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，10： | 7 |
| 8 | Suppressed expression of miR-378 targeting gzmb in N cells is required to control dengue virus infection显示文摘 | Shuyan Liu Lingming Chen Ying Zeng Lulu Si Xiaolan Guo Junmei Zhou Danyun Fang Gucheng Zeng Lifang Jiang | 2016 | Cellular & Molecular Immunology2016，13，5： | 7 |
| 9 | Highly conductive dodecaborate/MXene composites for high performance supercapacitors显示文摘With the increasingly prominent energy and environmental issues,the supercapacitors,as a highly efficient and clean energy conversion and storage devices,meet the requirements well.However,it is still a challenge to enhance the capacitance and energy density of supercapacitors.A novel and highly conductive dodecaborate/MXene composites have been designed for high performance supercapacitors.The surface charge property of MXene was modified by a simple ultrasonic treatment with ammonium ion,and the dodecaborate ion can be inserted into the inner surface of MXene by electrostatic adsorption.Due to the unique icosahedral cage conjugate structure formed by the B-B bond and the highly delocalized three-dimensionalπbond structure of the electrons,the negative charge is delocalied on the whole dodecaborate ion,which reduces the ability to bind to cations.Therefore,the cations can move easily,and the dodecaborate can act as a“lubricant”for ion diffusion between the MXene layers,which significantly improves the ion transfer rate of supercapacitors.The dodecaborate/MXene composites can achieve an extremely high specific capacitance of 366 F.g^-1 at a scan rate of 2 mV.s^-1,which is more than eight times higher than that of MXene(43 F1-)at the same scan rate.Our finding provides a novel route on the fabrication of the high performance supercapacitors. | Zhenxing Li Chang Ma Yangyang Wen Zhiting Wei Xiaofei Xing Junmei Chu Chengcheng Yu Kaili Wang Zhao-Kui Wang | 2020 | Nano Research2020，13，1： | 7 |
| 10 | The onset and advance of the Asian summer monsoon显示文摘Based on the daily outgoing longwave radiation (OLR) data from National Oceanic and At- mospheric Administration (NOAA) satellites, the Cli- mate Prediction Center’s merged analysis of pre- cipitation (CMAP) data and the National Centers for Environmental Prediction/National Center for At- mospheric Research (NCEP/NCAR) reanalysis dataset, the mean intraseasonal variability of the Asian summer monsoon (ASM) is investigated by using power spectrum analysis, band-pass filter, and diagnostic analyses. The processes of the onset and advance of monsoon over the southern part of Indo- china Peninsula, the east coast of Bay of Bengal, the South China Sea and the Indian subcontinent are explored. It is found that there is an abrupt change in OLR, precipitation and zonal wind during the onset and advance of the ASM. It is also indicated that the southern part of Indochina Peninsula and the adja- cent Andaman Sea is the region where the earliest onset of the ASM occurs in the 2nd pentad of May. | Lü Junmei ZHANG Qingyun TAO Shiyan JU Jianhua | 2006 | Chinese Science Bulletin2006，51，1： | 7 |
| 11 | Study on the Recovery of Rhodium from Spent Organic Rhodium Catalysts of Acetic Acid Industry Using Pyrometallurgical Process显示文摘A new process recycling rhodium from organic waste containing rhodium in acetic acid industry is developed. Use the special affinity of base metal sulfides (FeS, Ni2S3 , CuS, etc.) on platinum group metals, adopting high nickel matte trapping-aluminothermic activation method to recovery rhodium from incinerator residue of organic rhodium waste. The method is shorter process, lower equipment requirement, and the higher activity of rhodium black. In pyrometallurgy enrichment process,the recovery rate of rhodium reached 94.65%, the full flow of rhodium recovery rate was 92.04%. | HE Xiaotang WANG Huan WU Xilong LI Yong ZHAO Yu HAN Shouli LI Kun GUO Junmei | 2012 | 贵金属2012，33，A01： | 7 |
| 12 | Cobalt atoms dispersed on hierarchical carbon nitride support as the cathode electrocatalyst for high-performance lithium-polysulfide batteries显示文摘Lithium-sulfur batteries are promising candidates for next-generation energy storage but are confronted with several challenges. One of the possible solutions is to design proper cathode electrocatalysts to accelerate the redox interconversion of solvated polysulfide intermediates. Herein, we report cobalt atoms dispersed on hierarchical carbon nitride support as an effective cathode electrocatalyst for lithium-polysulfide batteries. The electrocatalyst material is prepared from the simple reaction between melamine and cyanuric acid in the presence of Co^2+, followed by the Ar annealing. The product has a unique hierarchical structure consisting of many thin and porous C3 N4 nanosheets finely dispersed with Co atoms. The atomic dispersion of Co species is confirmed by X-ray absorption experiments.Electrochemical measurements reveal that it can promote the interconversion of polysulfides. As a result,batteries using this cathode electrocatalyst achieve large capacity($1400 mAh/g at 1.6 mA/cm^2), good rate performance($800 mAh/g at 12.8 mA/cm^2) and impressive cycling stability under different current densities and different sulfur loadings. | Jialing Wu Junmei Chen Yang Huang Kun Feng Jun Deng Wei Huang Yunling Wu Jun Zhong Yanguang Li | 2019 | Science Bulletin2019，64，24： | 7 |
| 13 | Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry显示文摘The aim of this work was to develop an automated on-line solid phase extraction(SPE)with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish samples.Samples were extracted with 0.2%formic acid acetonitrile solution,purified by on-line SPE device with HLB column,then separated by XBridge C18 column,using 0.1%formic acid solution and acetonitrile as the mobile phase.Mass spectrometric data was acquired under multiple reaction monitoring(MRM)mode using positive ionization electrospray.Internal standard method was used in the quantification,good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than 0.9990.The limits of detection were in the range of 0.125–2.00g/kg and the limits of quantitation were in the range of 0.250–5.00g/kg.Recoveries of the method were in range of 78.3%–99.3%,relative standard deviation were lower than 10%.The method was simple,sensitivity,and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish. | Junmei Ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li | 2020 | Food Science and Human Wellness2020，9，4： | 6 |
| 14 | AaMYB3 interacts with AabHLH1 to regulate proanthocyanidin accumulation in Anthurium andraeanum(Hort.)—another strategy to modulate pigmentation显示文摘Proanthocyanidins(PAs),also known as“condensed tannins”,are colorless metabolites produced through the flavonoid pathway that are involved in stress resistance in plants.Because PAs are involved in the anthocyanin biosynthetic pathway,they play a role in the modification of pigmentation conferred by anthocyanins in ornamental organs.In this study,we isolated the gene and functionally characterized an R2R3-MYB transcription factor(TF),AaMYB3,and a basic helix-loop-helix TF,AabHLH1,from Anthurium andraeanum(Hort.),a typical tropical flower.AaMYB3 is primarily expressed in the spathe and negatively correlates with anthocyanin accumulation.A complementation test in an Arabidopsis tt8 mutant showed that AabHLH1 successfully restores the PA-deficient seed coat phenotype.The ectopic overexpression of AaMYB3 alone or its coexpression with AabHLH1 in transgenic tobacco resulted in light pink or even pale-pink corolla limbs by reducing their anthocyanin levels and greatly enhancing their accumulation of PAs.This overexpression of the anthurium TF genes upregulated the late anthocyanin enzymeencoding genes(NtDFR and NtANS)and the key PA genes(NtLAR and NtANR)in transgenic tobacco.The interaction between AaMYB3 and the AabHLH1 protein was confirmed using yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC)assays.In the developing red spathes of the cultivars“Vitara”and“Tropical”,the expression of AaMYB3 was closely linked to PA accumulation,and AaMYB3 was coexpressed with AaCHS,AaF3H,AaDFR,AaANS,AaLAR,and AaANR.The expression pattern of AabHLH1 was similar to that of AaF3′H.Our results suggest that AaMYB3 and AabHLH1 are involved in the regulation of PA biosynthesis in anthurium and could potentially be used to metabolically engineer PA biosynthesis in plants. | Chonghui Li Jian Qiu Surong Huang Junmei Yin Guangsui Yang | 2019 | Horticulture Research2019，6，1： | 6 |
| 15 | Vertical distribution of aerosol optical properties based on aircraft measurements over the Loess Plateau in China显示文摘Vertical distributions of aerosol optical properties based on aircraft measurements over the Loess Plateau were measured for the first time during a summertime aircraft campaign, 2013 in Shanxi, China. Data from four flights were analyzed. The vertical distributions of aerosol optical properties including aerosol scattering coefficients(σsc), absorption coefficients(σab),Angstr?m exponent(α), single scattering albedo(ω), backscattering ratio(βsc), aerosol mass scattering proficiency(Q sc) and aerosol surface scattering proficiency(Q sc′) were obtained. The mean statistical values of σsc were 77.45 Mm-1(at 450 nm), 50.72 Mm-1(at 550 nm), and32.02 Mm-1(at 700 nm). The mean value of σab was 7.62 Mm-1(at 550 nm). The mean values ofα, βsc and ω were 1.93, 0.15, and 0.91, respectively. Aerosol concentration decreased with altitude. Most effective diameters(ED) of aerosols were less than 0.8 μm. The vertical profiles of σsc,, α, βsc, Q sc and Q sc′ showed that the aerosol scattering properties at lower levels contributed the most to the total aerosol radiative forcing. Both α and βsc had relatively large values, suggesting that most aerosols in the observational region were small particles. The mean values of σsc, α, βsc, Q sc, Q sc′, σab and ω at different height ranges showed that most of the parameters decreased with altitude. The forty-eight hour backward trajectories of air masses during the observation days indicated that the majority of aerosols in the lower level contributed the most to the total aerosol loading, and most of these particles originated from local or regional pollution emissions. | Junxia Li Xingang Liu Liang Yuan Yan Yin Zhanqing Li Peiren Li Gang Ren Lijun Jin Runjun Li Zipeng Dong Yiyu Li Junmei Yang | 2015 | Journal of Environmental Sciences2015，27，8： | 6 |
| 16 | Baicalin influences the dendritic morphology of newborn neurons in the hippocampus of chronically stressed rats显示文摘Chronic stress models,established in adult Sprague-Dawley rats through a 14-day subcutaneous injection of 40 mg/kg corticosterone,once per day,were given a daily oral feeding of 50 mg/kg baicalin.The study was an attempt to observe the effect of baicalin on neurogenesis in chronically stressed rats.Results showed that subcutaneous injection of corticosterone significantly decreased the total number of doublecortin-positive neurons in the hippocampus.The reduced cell Ⅰ number caused by corticosterone was mainly due to the decrease of class Ⅱ doublecortin-positive neurons,but the class doublecortin-positive neurons were unaffected.Baicalin treatment increased the number of both class Ⅰ and class Ⅱ doublecortin-positive neurons.In addition,doublecortin-positive neurons showed less complexity in dendritic morphology after corticosterone injection,and this change was totally reversed by baicalin treatment.These findings suggest that baicalin exhibits a beneficial effect on adult neurogenesis. | Xinghua Jiang Junmei Xu Dingquan Zou Lin Yang Yaping Wang | 2013 | Neural Regeneration Research2013，8，6： | 6 |
| 17 | Superior growth performance in broiler chicks fed chelated compared to inorganic zinc in presence of elevated dietary copper显示文摘Background: The goal of this study was to compare the antagonism of elevated dietary Cu(250 mg/kg) from Cu SO4 on three different Zn sources(Zn SO4· H2O; [Zn bis(-2-hydroxy-4-(methylthio)butanoic acid)], Zn(HMTBa)2,a chelated Zn methionine hydroxy analogue; and Zn-Methionine), as measured using multiple indices of animal performance in ROSS 308 broilers.Methods: Three experiments were conducted in broiler chicks fed a semi-purified diet. Al birds were fed a Zn-deficient diet(8.5 mg/kg diet) for 1 wk, and then provided with the experimental diets for 2 wks.Results: Experiment 1 was a 2 × 2 factorial design with two levels of Cu(8 vs. 250 mg/kg diet from Cu SO4) and two Zn sources at 30 mg/kg [Zn SO4· H2 O vs. Zn(HMTBa)2]. Elevated Cu impaired growth performance only in birds fed Zn SO4.Compared to Zn SO4· H2 O, Zn(HMTBa)2improved feed intake(12 %; P < 0.001) and weight gain(12 %, P < 0.001) and the benefits were more pronounced in the presence of 250 mg/kg diet Cu. Experiment 2 was a dose titration of Zn SO4· H2 O and Zn(HMTBa)2at 30, 45, 60, and 75 mg/kg diet in the presence of 250 mg/kg Cu SO4. Feed:gain was decreased and tibia Zn was increased with increasing Zn levels from 30 to 75 mg/kg. Birds fed Zn(HMTBa)2consumed more food and gained more weight compared to birds fed Zn SO4, especially at lower supplementation levels(30 and45 mg/kg; interaction P < 0,05). Experiment 3 compared two organic Zn sources(Zn(HMTBa)2vs. Zn-Methionine)at 30 mg/kg with or without 250 mg/kg Cu SO4. No interactions were observed between Zn sources and Cu levels on performance or tissue mineral concentrations. High dietary Cu decreased weight gain(P < 0.01). Tibia Cu and liver Cu were significantly increased with 250 mg/kg dietary Cu supplementation(P < 0.01). No difference was observed between the two Zn sources.Conclusions: Dietary 250 mg/kg Cu significantly impaired feed intake and weight gain in birds fed Zn SO4· H2 O,but had less impact in birds fed Zn(HMTBa)2. No difference was observed between the two organic zinc sources.These results are consistent with the hypothesis that chelated organic Zn is better utilized than inorganic zinc in the presence of elevated Cu. | Junmei Zhao Robert B. Shirley Julia J. Dibner Karen J. Wedekind Frances Yan Paula Fisher Thomas R. Hampton Joseph L. Evans Mercedes Vazquez-Anon | 2016 | Journal of Animal Science and Biotechnology2016，7，4： | 6 |
| 18 | Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography显示文摘In this experiment,a liquid chromatography tandem mass spectrometry method was built to determine 15 pesticide residues in Chinese cabbage and cucumber samples based on online turbulent flow chromatography purification.After modified quick,easy,cheap,effective,rugged,and safe(QuEChERS)extraction,extracts were directly injected to the TLX(TurboFlow Liquid Xcalibur)system and brought to TurboFlow™columns for on-line purification and then transferred to analytical column for further separation and analysis.TurboFlow™columns types,transfer flow rate,and transfer time were optimized.Limits of detection and limits of quantification of the method obtained for 15 pesticide residues were ranged between 0.2–1.0μg/kg and 0.5–2.0μg/kg in Chinese cabbage and cucumber samples.Recoveries of pesticide residues were in range of 75.3%–103.7%.Matrix effects for 15 pesticides were in range of 5.6%–106.6%.The developed method has been successfully used for the determination of 15 pesticide residues in real samples. | Sufang Fan Junmei Ma Meirong Cao Juan Wang Leilei Zhang Yan Zhang Qiang Li Jia Chen | 2021 | Food Science and Human Wellness2021，10，1： | 5 |
| 19 | Enhanced nitrate reduction in water by a combined bio-electrochemical system of microbial fuel cells and submerged aquatic plant Ceratophyllum demersum显示文摘High nitrate(NO_3^-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO_3^-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO_3^- in different treatments were studied by adding quantitative^(15)NO_3^- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO_3^- from the sediment–water system.The complete removal rates of added^(15)N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO_3^- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO_3^- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode. | Peng Xu Enrong Xiao Junmei Wu Feng He Yi Zhang Zhenbin Wu | 2019 | Journal of Environmental Sciences2019，31，4： | 5 |
| 20 | Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny显示文摘The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with heterosis.Gene knockout technique provides a safe and acceptant way for fish breeding.Achieving gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization breeding.Tyrosinase(TYR) is a key enzyme in melanin synthesis.Depletion of tyr in zebrafish and mice results in mosaic pigmentation or total albinism.Here,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant WCC.Both the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,i.e.tyrp1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin synthesis.These results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical fish.Furthermore,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min Tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu | 2019 | Science China(Life Sciences)2019，62，9： | 4 |
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