|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Engineering application of membrane bioreactor for wastewater treatment in China: Current state and future prospect显示文摘中国是大规模膜生物反应器(MBR ) 的 forerunner 申请。自从第一大规模 MBR (10 000 m < 啜 class= “ a-plus-plus ” > 3 湩朠潯 ? 慦瑩? | Kang XIAO Ying XU Shuai LIANG Ting LEI Jianyu SUN Xianghua WEN Hongxun ZHANG Chunsheng CHEN Xia HUANG | 2014 | Frontiers of Environmental Science & Engineering2014，8，6： | 26 |
| 2 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 3 | Omics-based integrated analysis identified ATRX as a biomarker associated with glioma diagnosis and prognosis显示文摘Objective:ATRX is a multifunctional protein that is tightly regulated by and implicated in transcriptional regulation and chromatin remodeling.Numerous studies have shown that genetic alterations in ATRX play a significant role in gliomas.This study aims to further determine the relationship between ATRX and glioma prognosis and identify possible mechanisms for exploring the biological significance of ATRX using large data sets.Methods:We used The Cancer Genome Atlas(TCGA)database and 130 immunohistochemical results to confirm the difference in ATRX mutations in high-and low-grade gliomas.An online analysis of the TCGA glioma datasets using the cBioPortal platform was performed to study the relationship between ATRX mutations and IDH1,TP53,CDKN2 A and CDKN2 B mutations in the corresponding TCGA glioma dataset.In combination with clinical pathology data,the biological significance of the relationships were analyzed.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses and annotations of all adjacent genes in the network were performedin the Database for Annotation,Visualization and Integrated Discovery(DAVID)and R language.A protein-protein interaction(PPI)network was constructed,and the interactions of all adjacent nodes were analyzed by the String database and using Cytoscape software.Results:In the selected TCGA glioma datasets,a total of 2,228 patients were queried,21%of whom had ATRX alterations,which co-occurred frequently with TP53 and IDH1 mutations.ATRX alterations are associated with multiple critical molecular events,which results in a significantly improved overall survival(OS)rate.In low-grade gliomas,ATRX mutations are significantly associated with multiple important molecular events,such as ZNF274 and FDXR at mRNA and protein levels.A functional cluster analysis revealed that these genes played a role in chromatin binding and P53,and a link was observed between ATRX and IDH1 and TP53 in the interaction network.ATRX and TP53 are important nodes in the network and have potential links with the blood oxygen imbalance.Conclusions:ATRX mutations have clinical implications for the molecular diagnosis of gliomas and can provide diagnostic and prognostic information for gliomas.ATRX is expected to serve as a new therapeutic target. | Yingbin Xie Yanli Tan Chao Yang Xuehao Zhang Can Xu Xiaoxia Qiao Jianglong Xu Shaohui Tian Chuan Fang Chunsheng Kang | 2019 | Cancer Biology & Medicine2019，16，4： | 5 |
| 4 | MicroRNA and Brain Tumors显示文摘MicroRNAs(miRNAs)were first described in 1993 by Lee and col eagues,and the term microRNA was only introduced in 2001 in a set of three articles in Science[1].One of the biggest surprises in the past few years has been the emergence of miRNAs as a major new class of gene expression regulators.Recent studies suggest that miRNA alterations are involved in the initiation and progression of human cancer.The brain tumor, glioblastoma multiforme,is the most malignant and deadly form of gliomas. The prognosis is poor and the median survival with combined radiotherapy and chemotherapy is only 14.6 months.With the discovery of miRNA,the miRNA profiles may become useful biomarkers for brain tumor diagnostics, and miRNA therapy could be a powerful tool for brain tumor prevention and therapeutics.This review outlines the background of miRNA and its expression and therapeutic potential for brain tumors. | Xuan Zhou Chunsheng Kang Peiyu Pu | 2007 | Chinese Journal of Clinical Oncology2007，4，5： | 4 |
| 5 | siRNA epidermal growth factor receptor silencing in U251 glioma cells显示文摘BACKGROUND: Dicer, a large multidomain ribonuclease, is responsible for processing double-stranded RNAs (dsRNAs) to 20-bp-long small interfering RNAs (siRNAs), which act as effectors during RNA interference (RNAi). OBJECTIVE: To observe the efficacy of siRNA cocktails generated by recombinant human Dicer on the down-regulation of epidermal growth factor receptor (EGFR) expression in human glioma cells. DESIGN, TIME AND SETTING: The following in vitro experiment was performed at the Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute. MATERIALS: Mini-RNA isolation kit, human placenta complimentary DNA (cDNA) was produced by Tiangen Biotech (Beijing, China), human glioblastoma U251-MG cells were produced by the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. METHODS: A PCR product from the human EGFR, which corresponded to the tyrosine kinase domain of the 3'-end fragment, was used as the T7-promotor for in vitro transcription. siRNA cocktails were generated by in vitro dicing of double stranded RNA. A total of 500, 250 and 125 μg siRNA cocktails were transiently transfected into U251 glioma cells through the use of the GeneSilencer. MAIN OUTCOME MEASURE: Expression of EGFR was detected by real-time PCR. RESULTS: The total PCR product of the human EGFR, corresponding to the tyrosine kinase domain, is approximately 680 bp in length. The PCR transcriptants included GCC leader sequences and a T7 promoter sequence, with a fragment of EGFR cDNA at the center. The T7 promoter was prepared for in vitro transcription of dsRNA. After dicing for 24 hours, the 21-nt siRNA cocktails were verified by 4% agarose gel. The difference between threshold cycle of a sample assay and threshold cycle of the corresponding endogenous reference (ΔCt) among parental U251 cells and cells transfected with different doses of siRNA cocktails were determined to be 3.06, 7.35, and 10.31 cycles, respectively. These results indicated effective silencing of EGFR expression by siRNA cocktails through transient transfection. CONCLUSION: The siRNA cocktails significantly suppressed exogenous expression of EGFR in U251 glioblastoma cells in a dose-dependent manner, thereby providing a more promising RNAi methodology for glioma therapy. | Chunsheng Kang Zhiyong Zhang Zhifan Jia Qiang Huang Guangxlu Wang Mingzhe Qiu Peiyu Pu | 2008 | Neural Regeneration Research2008，3，6： | 2 |
| 6 | Advances in Technologies of Piezoelectric Pumping with Valves显示文摘Piezoelectric pump faces unprecedented challenges when higher expectation and requirements need to be met in their applications mainly to medical treatment,hygiene and public health,and preventive healthcare.Specifically,the piezoelectric pump with valve has the disadvantages of complex structure,high duty cycle of valves,and valve movement lagged behind piezoelectric ceramics oscillation.In an attempt to inhibit its shortcomings,some researchers presented novel concepts for structural design of piezoelectric pump with valve,which could become a new research focus.Among them,the investigation into various soft valves,represented by soft structure valves made of rigid materials and soft material valves made of flexible materials,has been fruitful in recent years.The integrated design of both material and structure can tackle the problems encountered in the study of piezoelectric pump with valve,thus simplifying the pump structure,reducing the duty-cycle of valves,and improving the lagging of valve motion.In addition,new inventions of pump structure have sprung up,such as the pumps containing a single-chamber with double-drive,single-chamber with single-drive in series and single-chamber with single-drive in parallel,as well as the mixed-chamber in series and parallel.After surveying the recent progresses made by dominant academia in the development of piezoelectric pump encompassing valve,with a particular emphasis on structure design of both valve and pump body,we also summarize and identify the future research directions. | Zhang Jianhui Wang Ying Fu Jun Yan Kang Li Zhiming Zhao Chunsheng | 2016 | Transactions of Nanjing University of Aeronautics and Astronautics2016，33，3： | 2 |
| 7 | Silencing of IKKε using siRNA inhibits proliferation and invasion ofglioma cells in vitro and in vivo显示文摘 | Huibing Li Lingchao Chen Anling Zhang Guangxiu Wang Lei Han Kai Yu Peiyu Pu Chunsheng Kang Qiang Huang | 2012 | International Journal of Oncology2012，，1： | 1 |
| 8 | Protective Effects of Ulinastatin on Proliferation and Cytokine Release of Splenocytes from Rats with Severe Acute Pancreatitis显示文摘 | Tao Ma Chunsheng Kang Hongwei Shao | 2006 | Eur Surg Res2006，38，4： | 1 |
| 9 | A method for real-time compensation of moving ferromagnet ' s magnetic moment 显示文摘 | Zhou Jianjun Lin Chunsheng Fu Kang | 2013 | Journal of Magnetism and Magnetic Materials2013，325，： | 1 |
| 10 | MicroRNA-221 and -222 Regulate Radiation Sensitivity by Targeting the PTEN Pathway显示文摘 | Chunzhi Zhang Chunsheng Kang Ping Wang Yongzhen Cao Zhonghong Lv Shizhu Yu Guangxiu Wang Anling Zhang Zhifan Jia Lei Han Chunying Yang Hiromichi Ishiyama Bin S. Teh Bo Xu Peiyu Pu | 2011 | International Journal of Radiation Oncology Biology Physics2011，，1： | 1 |
| 11 | Boosting of the enhanced permeability and retention effect with nanocapsules improves the therapeutic effects of cetuximab显示文摘Objective:The introduction of therapeutic antibodies(tAbs)into clinical practice has revolutionized tumor treatment strategies,but their tumor therapy efficiency is still far below expectations because of the rapid degradation and limited tumor accumulation of tAbs.Methods:We developed a nanocapsule-based delivery system to induce the self-augmentation of the enhanced permeability and retention(EPR)effect.This system constantly penetrated across the blood-tumor barrier into the tumor while avoiding the attack of tAbs by the immune system.The biodistribution and therapeutic effect were tested with single dose administration of nanocapsule-tAbs in vivo.Results:The accumulation of Nano(cetuximab)within subcutaneous PC9 tumors was gradually enhanced over 6 days after single dose administration,which was contrary to the biodistribution of native cetuximab.Nano(cetuximab)accumulated in tumor tissues via the EPR effect and released cetuximab.The released cetuximab acted on vascular endothelial cells to destroy the blood-tumor barrier and induce self-augmentation of the EPR effect,which in turn contributed to further tumor accumulation of long-circulating Nano(cetuximab).Compared with single dose administration of native cetuximab,Nano(cetuximab)showed an effective tumor suppressive effect for 3 weeks.Conclusions:The nanocapsule-based delivery system efficiently delivered tAbs to tum or tissues and released them to boost the EPR effect,which facilitated further tumor accumulation of the tAbs.This novel self-augmentation of the EPR effect facilitated by the biological characteristics of tAbs and nanotechnology contributed to the improvement of the therapeutic effect of tAbs,and stimulated new ideas for antibody-based tumor therapy. | Chao Yang Yanli Tan Hongzhao Qi Junhu Zhou Lixia Long Qi Zhan Yunfei Wang Xubo Yuan Chunsheng Kang | 2020 | Cancer Biology & Medicine2020，17，2： | 1 |
| 12 | Effect of Helicobacter pylori on cyclooxygenase-2 and inducible nitricoxide synthase in patients with gastric precancerous lesions and its clinicalsignificance显示文摘 | Hui Zhang Chunsheng Ding Zhimin Suo Yuhua Kang | 2015 | Experimental and Therapeutic Medicine2015，，6： | 1 |
| 13 | Preparation of carmustine-loaded PLA ultrasmallonanoparticles by adjusting mieellar behavior of surfactants显示文摘 | Yan Chenghu Yuan Xubo Kang Chunsheng | 2008 | Applied Polymer Science2008，110，4： | 1 |
| 14 | Co-delivery of as-miR-21 and 5-FU by poly(amidoamine) dendrimer attenuates human glioma cell growth in vitro显示文摘 | Ren Yu Kang ChunSheng Yuan Xubo | 2010 | J Biomater Sci2010，3，21： | 1 |
| 15 | Protective Effects of Ulinastatin on Proliferation and Cytokine Release of Splenocytes from Rats with Severe Acute Pancreatitis显示文摘 | Tao Ma Chunsheng Kang Hongwei Shao | 2006 | Eur Surg Res2006，38，10： | 1 |
| 16 | MiRNA-451 plays a role as tumor suppressor in human glioma cells显示文摘 | Yang Nan Lei Han Anling Zhang Guangxiu Wang Zhifan Jia Yang Yang Xiao Yue Peiyu Pu Yue Zhong Chunsheng Kang | 2010 | Brain Research2010，，： | 1 |
| 17 | Global changes of mRNA expression reveals an increased activity of theinterferon-induced signal transducer and activator of transcription (STAT) pathwayby repression of miR-221/222 in glioblastoma U251 cells显示文摘 | Chunzhi Zhang Lei Han Anling Zhang Weidong Yang Xuan Zhou Peiyu Pu Yue Du Huazong Zeng Chunsheng Kang | 2010 | International Journal of Oncology2010，，6： | 1 |
| 18 | A method for real-time compensation of moving ferromagnet's magnetic moment 显示文摘 | ZHOU Jianjun LIN Chunsheng FU Kang | 2013 | Journal of Magnetism and Mag-netic Materials2013，325，： | 1 |
| 19 | MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 inhuman glioma显示文摘 | Yuan Tian Yang Nan Lei Han Anling Zhang Guangxiu Wang Zhifan Jia Jianwei Hao Peiyu Pu Yue Zhong Chunsheng Kang | 2012 | International Journal of Oncology2012，，4： | 1 |
| 20 | Suppression of matrix metalloproteinase-9 expression by RNA interference inhibits SGC7901 gastric adenocarcinoma cell growth and invasion in vitro and in vivo显示文摘 | Fengjuan Zhao Qingyu Zhang Chunsheng Kang Xiaowei Cui Tao Wang Peng Xu Xuan Zhou Jian Liu Xiaomei Song | 2010 | Medical Oncology2010，，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员