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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | Adjuvant imatinib mesylate after resection of localised, primary gastrointestinal stromal tumour: a randomised, double-blind, placebo-controlled trial显示文摘 | Ronald P DeMatteo Karla V Ballman Cristina R Antonescu Robert G Maki Peter WT Pisters George D Demetri Martin E Blackstein Charles D Blanke Margaret von Mehren Murray F Brennan Shreyaskumar Patel Martin D McCarter Jonathan A Polikoff Benjamin R Tan Kouros | 2009 | The Lancet2009，，9669： | 2 |
| 3 | Signal peptidases显示文摘 | Paetzel M Karla A Strynadka N C Dalbey R E | 2002 | Chem Rev2002，102，： | 1 |
| 4 | Tau oligomers and aggregation in Alzheimer’s disease显示文摘 | Marco A.Meraz‐Ríos Karla I.Lira‐De León VictoriaCampos‐Pe?a Martha A.De Anda‐Hernández RaúlMena‐López | 2010 | Journal of Neurochemistry2010，，6： | 1 |
| 5 | Piezoelectric actuators as synthetic jets: Cavity dimension effects 显示文摘 | POORNA M KARLA M ALI R | 2007 | Journal of Intelligent Material Systems and Structures2007，18，11： | 1 |
| 6 | Effect of Inhibition Treatment, Type of Inocula, and Incubation Temperature on Batch H2 Production from Organic Solid Waste显示文摘 | Idania V V Elvira R L Karla M P | 2006 | Biotechnol Bioeng2006，95，3： | 1 |
| 7 | Molecular expression and functional evidence of a drug efflux pump (BCRP) in human corneal epithelial cells显示文摘 | Karla PK Earla R Boddu SH | 2009 | Curr Eye Res2009，34，1： | 1 |
| 8 | Inhibition of porcine endogenous retroviruses by RNA interference: increasing the safety of xenotransplantation 显示文摘 | Karlas A Kurth R Denner J | 2004 | Virology2004，325，1： | 1 |
| 9 | Isolation,Enzymatic Characterization and Action as Spreading Factor of a Hyaluronidase from Crotalus durissus terrificus Snake Venom显示文摘 | Karla C F Bordon MOrcio G Perino Jos6 R Giglio | 2012 | Toxicon2012，60，： | 1 |
| 10 | Direct Synthesis of A22O7 (A=Gd3+,Y3+) Solid Solutions by Ball Milling Constituent Oxides显示文摘 | Karla J Moreno Silva Rodrigo R Antonio F Fuentes | 2005 | Journal of Alloys and Compounds2005，390，： | 1 |
| 11 | The molecular structure of hop la-tent viroid(HLV),a new viroid occurring worldwide in hops显示文摘 | Holger P Karla R Heinz L S | 1988 | Nucleic Acids Research1988，16，10： | 1 |
| 12 | Molecular expression and functional evidence of a drug efflux pump(BCRP) in human corneal epithelial cells显示文摘 | Karla PK Earla R Boddu SH | 2009 | Curt Eye Res2009，34，1： | 1 |
| 13 | The functional role of C-reactive protein in aortic wall calcification显示文摘 | Warrier B Mallipeddi R Karla PK | 2005 | Cardiology2005，104，2： | 1 |
| 14 | Biodegradable polymers for the en- vironment 显示文摘 | Gross R A Karla B | 2002 | Science2002，297，5582： | 1 |
| 15 | Adjuvant imatinib mesylate after resection of localised, primary gastrointestinal stromal tumour: a randomised, double-blind, placebo-controlled trial显示文摘 | Ronald P DeMatteo Karla V Ballman Cristina R Antonescu Robert G Maki Peter WT Pisters George D Demetri Martin E Blackstein Charles D Blanke Margaret von Mehren Murray F Brennan Shreyaskumar Patel Martin D McCarter Jonathan A Polikoff Benjamin R Tan Kouros | 2009 | The Lancet2009，，9669： | 1 |
| 16 | A Dynamic Process Model of Service Quality:from Expectations to Behavioral Intentions显示文摘 | BOULIDING W KARLA A STAELIN R | 1997 | Journal of Marketing Research1997，30，1： | 1 |
| 17 | Effect of cigarette smoke on immune response: Exposure to cigarette smoke impairs antigen-mediated signaling in T cells and depletes IP3-sensitive Ca^2+ stores显示文摘 | Karla R Singh SP Savage SM Fujimoto K Suzuki T Kasahara T | 2000 | J Pharmacol Exp Ther2000，293，： | 1 |
| 18 | Left ventricular remodeling in transgenic mice with cardiac restricted overexpression of tumor necrosis factor显示文摘 | Mytsi L Karla M William R | 2001 | Circulation2001，104，： | 1 |
| 19 | Analysis of ISG expression in chronic hepatitis C identifies Viperin as a potential antiviral effector显示文摘 | Karla J Helbig Daryl T Y Lau Ljiljana Semendric Hugh A J Harley Michael R Beard | 2005 | Hepatology2005，42，3： | 1 |
| 20 | HIV-1infection of human macrophages directly induces Viperin which inhibits viral production显示文摘 | Najla Nasr Susan Maddocks Stuart G Turville Andrew N Harman Natalie Woolger Karla J Helbig John Wilkinson Chris R Bye Thomas K Wright Dharshini Rambukwelle Heather Donaghy Michael R Beard Anthony L Cunningham | 2012 | Blood2012，120，4： | 1 |
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