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| 1 | The resurgent landscape of xenotransplantation of pig organs in nonhuman primates显示文摘Organ shortage is a major bottleneck in allotransplantation and causes many wait-listed patients to die or become too sick for transplantation.Genetically engineered pigs have been discussed as a potential alternative to allogeneic donor organs.Although xenotransplantation of pig-derived organs in nonhuman primates(NHPs)has shown sequential advances in recent years,there are still underlying problems that need to be completely addressed before clinical applications,including(i)acute humoral xenograft rejection;(ii)acute cellular rejection;(iii)dysregulation of coagulation and inflammation;(iv)physiological incompatibility;and(v)cross-species infection.Moreover,various genetic modifications to the pig donor need to be fully characterized,with the aim of identifying the ideal transgene combination for upcoming clinical trials.In addition,suitable pretransplant screening methods need to be confirmed for optimal donor-recipient matching,ensuring a good outcome from xenotransplantation.Herein,we summarize the understanding of organ xenotransplantation in pigs-to-NHPs and highlight the current status and recent progress in extending the survival time of pig xenografts and recipients.We also discuss practical strategies for overcoming the obstacles to xenotransplantation mentioned above to further advance transplantation of pig organs in the clinic. | Xuan Zhang Quancheng Wang Jingjun Zhao Xiao Li Wei Peng Zhaoxu Yang Zhibin Lin Long Yang Rui Ding Kaishan Tao Kefeng Dou | 2021 | Science China(Life Sciences)2021，64，5： | 10 |
| 2 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 3 | CKIP-1 regulates macrophage proliferation by inhibiting TRAF6-mediated Akt activation显示文摘巨噬细胞在开发,动态平衡,织物修理和免疫起枢轴的作用。巨噬细胞增长被刺激殖民地的因素(M-CSF ) 导致了 Akt 发信号的巨噬细胞支持;然而,这个过程怎么被终止,仍然保持不清楚。这里,我们作为巨噬细胞增长的一个新奇禁止者识别酷蛋白 kinase 2-interacting protein-1 (CKIP-1 ) 。在放松巨噬细胞, CKIP-1 是在由组成地活跃的 GSK3β 的丝氨酸 342 点的 phosphorylated;, Akt 的下游的目标。这 phosphorylation 触发 CKIP-1 的 polyubiquitination 和 proteasomal 降级。在 M-CSF 刺激之上, Akt 被 CSF-1R-PI3K 激活然后使 GSK3β 失去活性;,导致 CKIP-1 和 β 的稳定; -catenin 蛋白质。β -catenin 包括 cyclin D 和 c-Myc 支持增长基因的表示。CKIP-1 与 TRAF6,为连接 K63 的 ubiquitination 要求的 ubiquitin ligase 和 Akt 的血浆膜招募交往,并且终止调停 TRAF6 的 Akt 激活。由这个工具, CKIP-1 在 M-CSF 刺激以后在迟了的阶段明确地禁止巨噬细胞增长。而且, CKIP-1 缺乏在老鼠自发地开发的 vitro 和 CKIP-1 −/− 导致增加的增长和巨噬细胞的减少的 apoptosis 巨噬细胞主导的脾大和 myeloproliferation。一起,这些数据证明 CKIP-1 由禁止调停 TRAF6 的 Akt 激活在巨噬细胞动态平衡的规定起一个关键作用。 | Luo Zhang Yiwu Wang Fengjun Xiao Shaoxia Wang Guichun Xing Yang Li Xiushan Yin Kefeng Lu Rongfei Wei Jiao Fan Yuhan Chen Tao Li Ping Xie Lin Yuan Lei Song Lanzhi Ma Lujing Ding Fuchu He Lingqiang Zhang | 2014 | Cell Research2014，24，6： | 7 |
| 4 | Efficacy of rigosertib, a small molecular RAS signaling disrupter for the treatment of KRAS-mutant colorectal cancer显示文摘Objective:Mutant KRAS,the principal isoform of RAS,plays a pivotal role in the oncogenesis of colorectal cancer by constitutively activating the RAF/MEK/ERK and PI3K/AKT pathways.Effective targeted therapies are urgently needed.We investigated whether rigosertib,a benzyl styryl sulfone RAS signaling disruptor,could selectively kill KRAS-mutant colorectal cancer cells.Methods:CCK-8 was used to determine the cell viability.Patient-derived tumor and cancer cell xenograft models were used to detect the inhibitory efficacy of rigosertib.Flow cytometry was used to evaluate the apoptosis and cell cycle progression.Apoptosis and cell cycle arrest markers were detected by Western blot.DCFH-DA was used to determine the reactive oxygen species.Immunohistochemistry staining and Western blot were performed to characterize RAS signaling markers in colorectal cancer tissues and cells.Results:Rigosertib(RGS)exhibited a cytotoxic effect against colorectal cancer cells,which was greater in KRAS-mutant cells.Furthermore,RGS induced mitotic arrest and oxidative stress-dependent apoptosis in KRAS-mutant DLD1 and HCT116 cells.Besides,RGS disrupted RAS signaling,and the inhibition of RAS/MEK/ERK was independent of cellular oxidative stress.Using patient-derived xenograft models,the response and tumor inhibition of RGS were significantly higher in the KRAS-mutant subgroup,while p-MEK,p-ERK,and p-AKT levels of RGS-treated tumors were significantly decreased.Finally,in a KRAS-mutant,chemotherapy-resistant patient-derived xenograft model,RGS showed a stronger therapeutic effect than the combination standard therapy involving fluoropyrimidine+oxaliplatin/irinotecan+bevacizumab.Conclusions:These data showed that targeting RAS signaling using RGS could be a therapeutic treatment for KRAS-mutant colorectal cancer patients. | Xinyi Zhou Qian Xiao Dongliang Fu Haochen Zhang Yang Tang Jinjie He Yeting Hu Xiangxing Kong Fei Teng Xiangrui Liu Ying Yuan Kefeng Ding | 2022 | Cancer Biology & Medicine2022，19，2： | 2 |
| 5 | Neuroendocrine Differentiation Is a Prognostic Factor for Stage II Poorly Differentiated Colorectal Cancer显示文摘 | Yue Liu Jinghong Xu Yurong Jiao Yeting Hu Chenghao Yi Qiong Li Zhou Tong Xiaowei Wang Lifeng Hu Qian Xiao Jun Li Kefeng Ding Hanlin L. Wang | 2014 | BioMed Research International2014，，： | 1 |
| 6 | Facile preparation of poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate)/Ag2Te nanorod composite films for flexible thermoelectric generator显示文摘In this work,poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)(PEDOT:PSS)coated silver telluride(PCeAg2Te)nanorods were prepared by a wet-chemical method using the PEDOT:PSS coated tellurium(PCeTe)nanorods as the templates.Flexible PCeAg2Te nanocomposite film on polyethersulfone(PES)substrate was prepared through vacuum filtration followed by mechanical pressing process.An optimal PCeAg2Te nanocomposite film showed a maximum power factor of 143.3 mW/mK^(2) at room temperature and 221.7 μW/mK^(2 ) at 373 K.The electrical conductivity of the PCeAg2Te composite film decreased by3.3%after bending for 1000 times around a rod with a radius of 5 mm.An eight-leg thermoelectric generator assembled with the optimal PCeAg2Te nanocomposite film generated a maximum output power and output density of 209.4 nW and 141.5 mW/cm^(2) at a temperature gradient of 30.3 K.This work provides a facile method to prepare thermoelectric materials for flexible thermoelectric generators. | Qiufeng Meng Haijun Song Yong Du Yufei Ding Kefeng Cai | 2021 | Journal of Materiomics2021，7，2： | 1 |
| 7 | Lectin-histochemical reactivity of sialic acid in breast cancer and its relationship to prognosis using limulus polyphemus agglutinin 显示文摘 | DING KEFENG YAMAGUCHI A GOI T | 1997 | Int J Oncology1997，10，： | 1 |
| 8 | Targeting RAS mutants in malignancies:successes,failures,and reasons for hope显示文摘RAS genes are the most frequently mutated oncogenes and play critical roles in the development and progression of malignancies.The mutation,isoform(KRAS,HRAS,and NRAS),position,and type of substitution vary depending on the tissue types.Despite decades of developing RAS-targeted therapies,only small subsets of these inhibitors are clinically effective,such as the allelespecific inhibitors against KRASG12C.Targeting the remaining RAS mutants would require further experimental elucidation ofRAS signal transduction,RASaltered metabolism,and the associated immune microenvironment.This study reviews the mechanisms and efficacy of novel targeted therapies for different RAS mutants,including KRAS allele-specific inhibitors,combination therapies,immunotherapies,and metabolism-associated therapies. | Hang Yang Xinyi Zhou Dongliang Fu Chenqin Le Jiafeng Wang Quan Zhou Xiangrui Liu Ying Yuan Kefeng Ding Qian Xiao | 2023 | Cancer Communications2023，43，1： | 0 |
| 9 | Mechanical properties of individual core-shell-structured Sn O2@C nanofibers investigated by atomic force microscopy and finite element method显示文摘Although SnO_2-based nanomaterials used to be considered as being extraordinarily versatile for application to nanosensors,microelectronic devices, lithium-ion batteries, supercapacitors and other devices, the functionalities of SnO_2-based nanomaterials are severely limited by their intrinsic vulnerabilities. Facile electrospinning was used to prepare SnO_2 nanofibers coated with a protective carbon layer. The mechanical properties of individual core-shell-structured SnO_2@C nanofibers were investigated by atomic force microscopy and the finite element method. The elastic moduli of the carbon-coated SnO_2 nanofibers remarkably increased, suggesting that coating SnO_2 nanofibers with carbon could be an effective method of improving their mechanical properties. | LIU Xing LI KeFeng DUAN XiaoLu HUANG YuTing LI Shuai LIU YiLin XU Fu LI Zheng ZHANG Ping DING YanHuai | 2018 | Science China(Technological Sciences)2018，61，8： | 0 |
| 10 | 基于临床特征的结直肠癌肝转移术后早期复发的预测模型显示文摘背景:接受根治性手术的结直肠癌肝转移(CRLM)患者预后较差且存在较大的异质性。早期复发是指根治性手术后6个月内出现的肿瘤复发,早期复发预后较差,需要早发现、早治疗。本研究旨在建立并验证基于临床特征的CRLM早期复发临床预测模型,并为术后随访策略提供新思路。方法:回顾性纳入2012-2019年间在本中心接受根治性手术的202例连续CRLM患者,并将其随机分为训练集(n=150)和验证集(n=52)。从病案系统中提取入组病例的基线信息及影像、病理、实验室检查资料。采用多因素逻辑回归分析明确早期复发的预测因素并建立列线图模型,再对模型的区分度、校准度及临床应用进行验证。结果:肝转移灶数目、疑似淋巴结转移、脉管侵犯、原发灶部位(结肠/直肠)、术前白蛋白及术后癌胚抗原(CEA)、糖类抗原19-9(CA19-9)为早期复发的独立预测因素。基于上述预测因素构建CRLM术后早期复发的预测模型。该模型在训练集和验证集中的曲线下面积(AUC)分别为0.866和0.792。基于本数据集,该模型对早期复发的预测价值显著高于临床风险评分和Beppu模型。Lift曲线显示,基于该模型的术后随访策略,可将风险最高的30%患者的早期复发检出率提高2倍。结论:本研究构建的CRLM患者根治性术后早期复发的预测模型具有较好的预测准确性,并有助于术后个体化随访策略的制定。 | Siqi Dai Yao Ye Xiangxing Kong Jun Li Kefeng Ding | 2021 | Gastroenterology Report2021，9，3： | 0 |
| 11 | Short-term tamoxifen administration improves hepatic steatosis and glucose intolerance through JNK/MAPK in mice显示文摘Nonalcoholic fatty liver disease (NAFLD) which is a leading cause of chronic liver diseases lacks effective treatment. Tamoxifen hasbeen proven to be the first-line chemotherapy for several solid tumors in clinics, however, its therapeutic role in NAFLD has neverbeen elucidated before. In vitro experiments, tamoxifen protected hepatocytes against sodium palmitate-induced lipotoxicity. Inmale and female mice fed with normal diets, continuous tamoxifen administration inhibited lipid accumulation in liver, andimproved glucose and insulin intolerance. Short-term tamoxifen administration largely improved hepatic steatosis and insulinresistance, however, the phenotypes manifesting inflammation and fibrosis remained unchanged in abovementioned models. Inaddition, mRNA expressions of genes related to lipogenesis, inflammation, and fibrosis were downregulated by tamoxifentreatment. Moreover, the therapeutic effect of tamoxifen on NAFLD was not gender or ER dependent, as male and female mice withmetabolic disorders shared no difference in response to tamoxifen and ER antagonist (fulvestrant) did not abolish its therapeuticeffect as well. Mechanistically, RNA sequence of hepatocytes isolated from fatty liver revealed that JNK/MAPK signaling pathwaywas inactivated by tamoxifen. Pharmacological JNK activator (anisomycin) partially deprived the therapeutic role of tamoxifen intreating hepatic steatosis, proving tamoxifen improved NAFLD in a JNK/MAPK signaling-dependent manner. | Zhiqiang Fang Hao Xu Juanli Duan Bai Ruan Jingjing Liu Ping Song Jian Ding Chen Xu Zhiwen Li Kefeng Dou Lin Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 0 |
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