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| 1 | Generation of cytotoxic T cell against HBcAg using retrovirally transduced dendritic cells显示文摘AIM: Cytotoxic T lymphocytes (CTLs) play an important role in resolving HBV infection. In the present study, we attempted to evaluate the efficiency of bone marrow-derived dendritic cells (DCs) transduced with recombinant retroviral vector bearing hepatitis B virus (HBV) core gene and the capability of generating CTLs against HBcAg by genetically modified DCs in vivo.METHODS: A retroviral vector containing HBV core gene was constructed. Replicating DC progenitor of C57BL/6 mice was transduced by retroviral vector and continually cultured in the presence of recombinant mouse granulocytemacrophage colony-stimulating factor (rmGM-CSF) and interleukin-4(IL-4) for 6 days. LPS was added and cultured for additional two days. The efficiency of gene transfer was determined by PCR, Western blot and FACS. Transduced DCs immunized C57BL/6 mice subcutaneously 2 times at an oneweek interval. Intracellular IFN-γ and IL-4 of immunized mice lymphocytes were analyzed. Generation of CTLs in lymphooytes stimulated with mitomycin C-treated EL4-C cell which stably expresses HBcAg was determined by LDH release assays.RESULTS: Recombinant retroviral expression vector (pLCSN) was positively detected by PCR as well as enzyme digestion with EcoRI and BamH I. Retroviruses were generated by pLCSN transfection packing cell and the virus titer was 3x10s CFU/ml. Indirect immunofluorescence and FACS showed that HBV core gene was expressed in murine fibroblasts. Transduced bone marrow cells had capability of differentiating into DCsc in vitro in the presence of rmGMCSF and rmIL-4. The result of PCR showed that HBV core gene was integrated into the genome of transduced DCs.Western blot analysis showed that HBV core gene was expressed in DCs. The transduction rate was 28 % determined by FACS. Retroviral transduction had no influence on DCs expressions of CD80 and MHC class Ⅱ. HBcAg specific CTLs and Th1 type immune responses could be generated in the mice by using transduced DCs as antigen presenting cells (APCs).CONCLUSION: Retroviral transduction of myeloid DCs progenitors expresses efficiently HBcAg, and genetically modified DCs evoke a higher CTLs response than HBcAg in vivo. | Chuan-LinDing KunYao Tian-TaiZhang FengZhou LinXu Jiang-YingXu | 2003 | World Journal of Gastroenterology2003，9，7： | 18 |
| 2 | Isolation of Nanjing Local Strains of HHV-7 and Their Biological and Immunological Characteristics显示文摘To investigate the biological and immunological characteristics of the Nanjing local strains of HHV7, four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffering from a kidney disease in Nanjing. The viruses were identified by transmission electron microscopy(EM), indirect immunofluorescence assay (IFA) with a specific monoclonal antibody; nested polymerase chain reaction, restriction mapping and DNA sequencing. The virus-infected cells showed the typical cytophathic effect (CPE) under microscopy and could be detected by IFA with the human herpesvirus-7 (HHV-7) specific antibody. Under EM, herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm.HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA sequencing. Similarly to DC strain, an known HHV-7 strain used in the present study as the positive control, the virus could be inactivated by ultraviolet irradiation for 10 min, heated at 45℃ for 30 min, pH<5 or>9 at 4℃ for 2 h and ether or chloroform for 10 h. The virus induced the production of TNF-α, IL-10 and IL-12p70 while inhibited IFN-γ secretion, increased the percentage of CD2+ cells while decreased that of CD4+ or CD45RA+ cells. The results indicate that the viruses isolated in Nanjing are HHV-7, which has similar biological characteristic to the known HHV-7 strain, DC. Infection with HHV-7 in vitro could affect immune function of lymphocytes by disturbing cytokine production and CD antigen expression. Cellular & Molecular Immunology. 2004; 1(5):367-372. | KunYao JieDou JianXu HuaSun GuangyongPeng QiangRen XiaohuiJi | 2004 | Cellular & Molecular Immunology2004，1，5： | 2 |
| 3 | Investigation of antimicrobial resistance in Escherichia coli and enterococci iso- lated from tibetan pigs显示文摘 | Li Peng Wu Dongfang Liu Kunyao | 2014 | PLoS One2014，9，4： | 1 |
| 4 | Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China显示文摘 | Ruichao Li Jing Lai Yang Wang Shuliang Liu Yun Li Kunyao Liu Jianzhong Shen Congming Wu | 2013 | International Journal of Food Microbiology2013，，1： | 1 |
| 5 | Interaction between M-CSF and IL-10 on productions ofIL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes显示文摘AIM: To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD 14, CD23, and CD64 by human monocytes. METHODS: Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, the culture supernatants and cells were separated and collected. IL-12P40 and IL-18 levels in the supernatants were determined by ELISA and the percentages of CD 14, CD23, and CD64 positive cells were examined by flow cytometry. RESULTS: (1) IL-10 decreased M-CSF-induced IL-18 levels, while M-CSF further reduced IL-12P40 level in the culture supernatants of IL-10-treated monocytes; (2) IL-l0 alone had no effect on the percentage of CD14-positive cells, but further increased the percentage of CD14-positive cells induced by M-CSF; M-CSF alone had no effect on the percentage of CD64-positive cells, but further increased the percentage of CD64-positive cells induced by IL-10, (3) IL-10 decreased the percentage of CD23-positive cells induced by M-CSF. CONCLUSION: Between M-CSF and IL-10, there were antagonistic effects on inducing IL-18 and CD23 expressions by monocytes, there were also synergistic effects on inhibiting IL-12P40 production and inducing CD14 and CD64 expressions by monocytes. | Xiao-huiJI TingYAO Jun-chuanQIN Shu-kuiWANG Hui-juanWANG KunYAO | 2004 | Acta Pharmacologica Sinica2004，25，10： | 1 |
| 6 | Synthesis and Luminescence Properties of Green-emitting Phosphor CaSc_(2)O_(4):Ce^(3+)显示文摘Using CaCO_(3),Sc_(2)O_(3),and CeO_(2) as raw materials,we prepared the CaSc_(2)O_(4):Ce^(3+)green phosphors for white light LEDs via a high-temperature solid-phase method in a reducing atmosphere.X-ray diffraction(XRD),field emission scanning electron microscopy(FESEM),fluorescence spectroscopy and other means were used to characterize the phase structure,apparent morphology,and photoluminescence properties of the product.CaSc_(2)O_(4) was synthesized under a reducing atmosphere(95%N_(2)+5%H_(2))at 1400℃ for 4 hours.Upon excitation with a blue light(450 nm wavelength),CaSc_(2)O_(4):Ce^(3+)displays a broadband green emission peak at 525 nm.The color coordinates of CaSc_(2)O_(4):7%Ce^(3+)emission peak are(0.3712,0.5940),and the product morphology is 1μm granular.Hence,it acts as a green phosphor suitable for white LEDs that can be excited by blue light. | 李兆 WU Kunyao WANG Yanan CAO Jing WANG Yongfeng LU Yuanyuan | 2022 | Journal of Wuhan University of Technology(Materials Science)2022，37，4： | 1 |
| 7 | Preliminary Discussion on Comprehensive Utilization of Tailings显示文摘Tailings produced in a concentration plant are the discharge of solid wastes after grinding ore into size and selecting useful components in the specific economic and technological condition. According to statistics, for the mining of metal ore, non-metallic ore, coal, clay, etc, the production of tailings of the world is up to 100 million tons per year. The number of existing tailing piles is 12718 in China, of which the construction ones are 1526, accounting for 12% of the total, and the closed tailing piles are 1024, accounting for 8%. As of 2007, the national total tailings accumulation is 8.046 billion tons. The non-ferrous metal mining is one of the largest discharge of solid wastes industries because of its low comprehensive recovery rate. For example, the beneficiation and recovery rate of non-ferrous metal mine is from 50% to 60% in China, which is lower 10% to 15% than developed countries, and the associated non-ferrous metal recovery rate is 40%, which is lower 20% than developed countries. On the utilization of duns, Poland is 90% to 100%, United States, Australia, France, Canada, Belgium and other countries followed, while China is only about 20%. The utilization of fly ash is to 100% in Japan and Denmark, France is 65%, UK is 55%, and China is just 45%. Quantities of waste rock, waste slag, and waste water have occupied land, destructed vegetation, deteriorated the soil and water quality, and caused land subsidence, landslides, mud-flow and other geological disasters. Therefore, the comprehensive utilization of resources is the right choice of mining sustainable development, environment protection, resource conservation, economic and social development. | WANG Fudong ZHU Xiaoqing WANG Zhonggang HAN Tao LI Zengsheng SUN Xiaohui LING Kunyao | 2013 | 矿物学报2013，33，S1： | 0 |
| 8 | WO_3 Anodic Oxide Film——I.Electrochromism and Auto-bleaching mechanism显示文摘Electrochromic and auto-bleaching processes at the WO2 anodic film in 0. 5 mol/L H2SO4 solution were investigated by cyclic voltammetry, a. c. impedance technique and photocurrent spectrometry. The colouration mechanism consists of hydrogen adsorption on the WO2 surface and the transport of H atoms in the WO, lattice. The bleaching process involves at least two steps: transport of interstitial H atoms and hydrogen desorption on the W surface, resulting in interstitial H+ ions; then extration of the H+ ions driven by the external electric field. The auto-bleaching arises from the hydroxylation due to both partial interstitial H atoms and a little of water contained in the film. | Lin Zhonghua, Chen Kunyao, Zheng Zhizhen, Chen Haiyi (Dept. of Chem. and Inst. of Phys. Chem. , Xiamen University, Xiamen) | 1990 | Chemical Research in Chinese Universities1990，6，4： | 0 |
| 9 | Special Care Behind the Walls显示文摘In western Henan Province,there is a prison which incarcerates some 100 people,convicted of one crime or another.They belong to different minority ethnic groups,such as Hui (Muslim),Uygur and Zhuang.As such they enjoy special care from the prison adminis- tration.The following are some stories about how they are treated in the prison: | ZHANG NING,LU KUNYAO & GUO TONGDING | 2007 | The Journal of Human Rights2007，6，6： | 0 |
| 10 | Incidence of HBV variants with a mutation at nt551 among hepatitis B patients in Nanjing and its neighbourhood显示文摘AIM: To investigate the epidemiology of hepatitis B virus (HBV) strains with a mutation at nt551 in surface gene among hepatitis B patients in Nanjing and its neighbourhood.METHODS: By using mutation-specific polymerase chain reaction (msPCR) established by our laboratory for amplifying HBV DNAs with a mutation at nt551, 117 serum samples taken from hepatitis B patients were detected.RESULTS: The results showed that 112 samples were positive for nt551A, 4 samples were positive for nt551G.One sample was positive for nt551T. No nt551C of HBV DNA was found. The incidence of HBsAg mutants with G,C, T, A at nt551 among 117 samples was 3.42%, 0%, 0.85%,95.73%, respectively.CONCLUSION: In Nanjing and its neighbourhood, hepatitis B patients are mainly infected with wild genotype HBV.The incidence of mutants with a mutation at nt551 in HBV genome is significantly lower than that in wild genotype HBV DNA (P<0.01). The necessity of adding components of HBsAg mutants to HBV vaccine needs further investigation. | Chun-LingMa De-XingFang KunYao Fa-QingLi Hui-YingJin Su-QinLi Wei-GuoTan | 2005 | World Journal of Gastroenterology2005，11，2： | 0 |
| 11 | Preparation of Flower-like Copper Foam Supported Co_(3)O_(4) Electrocatalyst and Its Hydrogen Evolution Performance显示文摘Flower-like copper foam Co_(3)O_(4) catalysts(Co_(3)O_(4)/CF) were prepared by hydrothermal method.The crystalline structure and microscopic morphology of the prepared samples were characterized by using X-ray diffractometer(XRD) and scanning electron microscope(SEM),and the electrochemical properties were investigated by an electrochemical workstation.The experimental results show that the Co_(3)O_(4) catalysts are successfully prepared on the foamed copper support by hydrothermal method,and the material’s morphology is mainly flower cluster.When the current density is 10 mA·cm^(-2),the overpotential value of the Co_(3)O_(4)/CF catalyst is 141 mV,lower than that of blank support.The electrochemical impedance(EIS) spectrum shows that the R_(ct )value of the Co_(3)O_(4)/CF catalyst decreases,and the Coulomb curves of double-layer show that the electrochemically active area of the Co_(3)O_(4)/CF catalyst efficiently increases compared with that of the blank support.Therefore,the as-obtained Co_(3)O_(4)/CF catalyst exhibits a good hydrogen evolution rate,showing great applicability potential in the catalytic electrolysis of water for hydrogen production. | 李兆 CHENG Julong WANG Yanan WU Kunyao CAO Jing | 2024 | Journal of Wuhan University of Technology(Materials Science)2024，39，2： | 0 |
| 12 | Exploration on the Future Development Path of Chinese Podcast显示文摘When Chinese podcast experienced a short period of rapid development in the first year and entered the“cooling-off period”,there are so many new exposed problems that need to be solved.In this paper,the author will explore the problems existing in the current development of Chinese podcast from six macro perspectives,as well as the possible trend of future podcast development. | ZHANG Pei XIONG Kunyao ZHOU Shanrong | 2022 | Cultural and Religious Studies2022，10，5： | 0 |
| 13 | Bifunctional fluoropyridinium-based cationic electrolyte additive for dendrite-free Li metal anode显示文摘Although lithium metal has become a promising anode material for high-energy batteries owing to its high specific capacity and the lowest reduction potential,the continuous side reactions with electrolyte as well as the safety problem caused by Li dendrite growth restrict Li anode’s practical application.Herein,we demonstrate that N-fluoropyridinium(ArF^(+))bis(trifluoromethane)sulfonimide(TFSI-)as an electrolyte additive can protect the lithium metal by both solid electrolyte interphase(SEI)protection and electrostatic repulsion mechanisms.The ArF+cations not only participate in forming F,Ncontaining SEI protective layer on Li surface,but also act as a cationic repellent during Li deposition to inhibit Li dendrite growth.As a result,the cycle performance of Li symmetric cells and Li||LiFePO_(4)full cells were significantly improved by using ArFTFSIadded electrolyte.This study provides an electrolyte additive strategy for Li anode realizing SEI protection and electrostatic repulsion simultaneously. | Kunyao Peng Pei Tang Qianqian Yao Qingyun Dou Xingbin Yan | 2023 | Nano Research2023，16，7： | 0 |
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