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1Multi-block SSD based on small object detection for UAV railway scene surveillance显示文摘A method of multi-block Single Shot Multi Box Detector(SSD)based on small object detection is proposed to the railway scene of unmanned aerial vehicle surveillance.To address the limitation of small object detection,a multi-block SSD mechanism,which consists of three steps,is designed.First,the original input images are segmented into several overlapped patches.Second,each patch is separately fed into an SSD to detect the objects.Third,the patches are merged together through two stages.In the first stage,the truncated object of the sub-layer detection result is spliced.In the second stage,a sub-layer suppression and filtering algorithm applying the concept of non-maximum suppression is utilized to remove the overlapped boxes of sub-layers.The boxes that are not detected in the main-layer are retained.In addition,no sufficient labeled training samples of railway circumstance are available,thereby hindering the deployment of SSD.A two-stage training strategy leveraging to transfer learning is adopted to solve this issue.The deep learning model is preliminarily trained using labeled data of numerous auxiliaries,and then it is refined using only a few samples of railway scene.A railway spot in China,which is easily damaged by landslides,is investigated as a case study.Experimental results show that the proposed multi-block SSD method produces an overall accuracy of 96.6%and obtains an improvement of up to 9.2%compared with the traditional SSD.Yundong LI Han DONG Hongguang LI Xueyan ZHANG Baochang ZHANG Zhifeng XIAO 2020Chinese Journal of Aeronautics2020,33,6:19
2Effect of Combined Application of Organic Fertilizer and Chemical Fertilizer in Different Ratios on Growth,Yield and Quality of Fluecured Tobacco显示文摘The effect of different ratio of organic and inorganic fertilizers on the growth and the yield and quality of flue-cured tobacco was studied.Using randomized blocks design,the data of the test materials K326 were analyzed by using variance analysis,significance test and other methods.The release efficiency of organic fertilizer in the soil was slower,and the effect of organic fertilizer on the growth of tobacco plants was worse than that of chemical fertilizers,and under 25% organic fertilizer + 75% chemical fertilizer treatment,the quality of the fluecured tobacco was good.Under the treatment of 50% organic fertilizer + 50% chemical fertilizer,the production of flue-cured tobacco was high and the ratio of first-class tobacco was high.It is suggested that the organic fertilizer application ratio should be 25%-50% in the agricultural production.Bing LIANG Kun HUANG Yali FU Hongguang LI Chi WANG Xiaotian ZHONG Qinghua LU Chengxiao HU 2017Asian Agricultural Research2017,9,12:9
3Use of genotype-environment interactions to elucidate the pattern of maize root plasticity to nitrogen deficiency显示文摘Maize(Zea mays L.) root morphology exhibits a high degree of phenotypic plasticity to nitrogen(N) de ficiency,but the underlying genetic architecture remains to be investigated Using an advanced BC_4F_3 population,we investigated the root growth plasticity under two contrasted N levels and identi fied the quantitative trait loci(QTLs) with QTL-environment(Q×E)interaction effects. Principal components analysis(PCA) on changes of root traits to N de ficiency(D LN-HN) showed that root length and biomass contributed for 45.8% in the same magnitude and direction on the first PC,while root traits scattered highly on PC_2 and PC_3. Hierarchical cluster analysis on traits for D LN-HN further assigned the BC_4F_3 lines into six groups,in which the special phenotypic responses to N de ficiency was presented These results revealed the complicated root plasticity of maize in response to N de ficiency that can be caused by genotype environment(G×E) interactions. Furthermore,QTL mapping using a multi-environment analysis identi fied 35 QTLs for root traits. Nine of these QTLs exhibited signi ficant Q×E interaction effects. Taken together,our findings contribute to understanding the phenotypic and genotypic pattern of root plasticity to N de ficiency,which will be useful for developing maize tolerance cultivars to N de ficiency.Pengcheng Li Zhongjuan Zhuang Hongguang Cai Shuai Cheng Ayaz Ali Soomro Zhigang Liu Riliang Gu Guohua Mi Lixing Yuan Fanjun Chen 2016Journal of Integrative Plant Biology2016,58,3:8
4Application of the quadrilateral area coordinate method:a new element for laminated composite plate bending problems显示文摘最近,一些新四边的有限元素被四边的区域坐标(QAC ) 方法成功地开发。与用 isoparametric 坐标的那些传统的模型相比,这些新模型对网孔失真不太敏感。在这篇论文,一新基于排水量, 4 节点 20-DOF (5-DOF 每节点) 四边的弯曲元素基于一阶砍为任意的把压成薄片的合成盘子的分析的变丑理论被介绍。它的弯曲部分基于元素 AC-MQ4,高效的 Mindlin-Reissner 板元素由 QAC 方法提出了的最近开发和概括一致条件方法;并且它的在里面飞机排水量地被双线性的形状功能在 isoparametric 坐标插入内推。而且,混合 processing 以后过程,被作者第一建议,再被采用改进压力答案,为特别横向砍压力。产生元素,作为 AC-MQ4-LC 表示了,在所有线性静态、动态的数字例子展出优秀性能。它再证明 QAC 方法,概括一致条件方法,和混合 processing 以后过程是为开发简单、有效、可靠的有限元素模型的有效工具。Song Cen Xiangrong Fu Yuqiu Long Hongguang Li Zhenhan Yao 2007Acta Mechanica Sinica2007,23,5:6
5Dark matter direct search sensitivity of the PandaX-4T experiment显示文摘The Panda X-4T experiment, a 4-ton scale dark matter direct detection experiment, is being planned at the China Jinping Underground Laboratory. In this paper we present a simulation study of the expected background in this experiment. In a 2.8-ton fiducial mass and the signal region between 1-10 keV electron equivalent energy, the total electron recoil background is found to be 4.9 × 10^(-5) kg^(-1) d^(-1) keV^(-1). The nuclear recoil background in the same region is 2.8 × 10^(-7) kg^(-1) d^(-1) keV^(-1). With an exposure of 5.6 ton-years, the sensitivity of Panda X-4 T could reach a minimum spin-independent dark matter-nucleon cross section of 6 × 10^(-48) cm^2 at a dark matter mass of 40 Ge V/c^2.HongGuang Zhang Abdusalam Abdukerim Wei Chen Xun Chen YunHua Chen XiangYi Cui BinBin Dong DeQing Fang ChangBo Fu Karl Giboni Franco Giuliani LinHui Gu XuYuan Guo ZhiFan Guo Ke Han ChangDa He ShengMing He Di Huang XingTao Huang Zhou Huang Peng Ji XiangDong Ji YongLin Ju ShaoLi Li Yao Li Heng Lin HuaXuan Liu JiangLai Liu YuGang Ma YaJun Mao KaiXiang Ni JinHua Ning XiangXiang Ren Fang Shi AnDi Tan AnQing Wang Cheng Wang HongWei Wang Meng Wang QiuHong Wang SiGuang Wang XiuLi Wang XuMing Wang Zhou Wang MengMeng Wu ShiYong Wu JingKai Xia MengJiao Xiao PengWei Xie BinBin Yan JiJun Yang Yong Yang ChunXu Yu JuMin Yuan JianFeng Yue Dan Zhang Tao Zhang Li Zhao QiBin Zheng JiFang Zhou Ning Zhou XiaoPeng Zhou 2019Science China(Physics,Mechanics & Astronomy)2019,62,3:6
6Haze removal for UAV reconnaissance images using layered scattering model显示文摘During the unmanned aerial vehicles(UAV) reconnaissance missions in the middle-low troposphere, the reconnaissance images are blurred and degraded due to the scattering process of aerosol under fog, haze and other weather conditions, which reduce the image contrast and color fidelity. Considering the characteristics of UAV itself, this paper proposes a new algorithm for dehazing UAV reconnaissance images based on layered scattering model. The algorithm starts with the atmosphere scattering model, using the imaging distance, squint angle and other metadata acquired by the UAV. Based on the original model, a layered scattering model for dehazing is proposed. Considering the relationship between wave-length and extinction coefficient, the airlight intensity and extinction coefficient are calculated in the model. Finally, the restored images are obtained. In addition, a classification method based on Bayesian classification is used for classification of haze concentration of the image, avoiding the trouble of manual working. Then we evaluate the haze removal results according to both the subjective and objective criteria. The experimental results show that compared with the origin image, the comprehensive index of the image restored by our method increases by 282.84%, which proves that our method can obtain excellent dehazing effect.Huang Yuqing Ding Wenrui Li Hongguang 2016Chinese Journal of Aeronautics2016,29,2:6
7Comparative investigation on soil salinity leaching under subsurface drainage and ditch drainage in Xinjiang arid region显示文摘This study was carried out to explore the effects of leaching salinity under subsurface drainage and mulched drip irrigation on saline and alkaline land from the year 2012 to 2014 in Xinjiang Region of China.Three sampled points were both set up in the subsurface drainage and ditch drainage areas.Soil samples were obtained at varied depths.Through observing the underground water table under each sampled point and measuring the electrical conductivity(EC)of the soil extracts,the following results were obtained:(1)after draining,the underground water table ranged from 1.6 m to 2.2 m in the ditch drainage area,and ranged from 1.5 m to 2.2 m in the subsurface drainage area.Thus,both irrigations could control underground water table below 1.5 m which is deeper than the main water-absorbing layers of crop root systems;(2)for subsurface drainage,the closer to the pipe,the better to leach salinity;decreased from the initial 13.54-22.95 g/kg to 8.20-11.47 g/kg;(3)compared with the amounts in 2012,soil total salt at each sampling point at depths of 0-200 cm in subsurface drainage area decreased by 42.99%,36.84%and 24.41%respectively in 2014;and in ditch drainage area decreased by 46.85%,38.12%and 30.80%respectively in 2014.The results showed both ditch and subsurface drainage could leach salinity effectively.He Xinlin Liu Hongguang Ye Jianwei Yang Guang Li Mingsi Gong Ping Aernaguli Aimaiti 2016International Journal of Agricultural and Biological Engineering2016,9,6:6
8Constraint Force Analysis of Metamorphic Joints Based on the Augmented Assur Groups显示文摘In order to obtain a simple way for the force analysis of metamorphic mechanisms, the systematic method to unify the force analysis approach of metamorphic mechanisms as that of conventional planar mechanisms is proposed. A force analysis method of metamorphic mechanisms is developed by transforming the augmented Assur groups into Assur groups, so that the force analysis problem of metamorphic mechanisms is converted into the force analysis problems of conventional planar mechanisms. The constraint force change rules and values of metamorphic joints are obtained by the proposed method, and the constraint force analysis equations of revolute metamorphic joints in augmented Assur group RRRR and prismatic metamorphic joints in augmented Assur group RRPR are deduced. The constraint force analysis is illustrated by the constrained spring force design of paper folding metamorphic mechanism, and its metamorphic working process is controlled by the spring force and geometric constraints of metamorphic joints. The results of spring force show that developped design method and approach are feasible and practical. By transforming augmented Assur groups into Assur groups, a new method for the constraint force analysis of metamorphic joints is proposed firstly to provide the basis for dynamic analysis of metamorphic mechanism.LI Shujun WANG Hongguang YANG Qiang 2015Chinese Journal of Mechanical Engineering2015,28,4:5
9Practical Structural Design Approach of Multiconfiguration Planar Single‑Loop Metamorphic Mechanism with a Single Actuator显示文摘As a type of multiconfiguration mechanism that can operate in an under-actuated state,metamorphic mechanisms were proposed more than two decades ago and attracted significant interest.Studies on structural synthesis of metamorphic mechanisms tend to focus more on metamorphic techniques and the structural synthesis of source mechanisms for metamorphic mechanisms.By designing different constraint architectures of metamorphic joints,multistructures can be obtained from the same source metamorphic mechanism.To determine the constraint architectures of metamorphic joints and their different assembly combinations,a kinematic status matrix and a corresponding constraint status matrix are constructed based on the metamorphic cyclogram of a source mechanism.According to the equivalent resistance gradient model and the constraint status matrix,an equivalent resistance matrix for the metamorphic joints is proposed.A structural synthesis matrix of the metamorphic mechanism is then obtained from the equivalent resistance matrix by deducing the constraint form vectors of the metamorphic joints.Furthermore,a kinematic diagram synthesis of the source metamorphic mechanism of a planar single-loop metamorphic mechanism is proposed,which is based on only the 14 one-or zero-degrees-of-freedom linkage groups.The entire structural design method of a metamorphic mechanism is based on the structural synthesis matrix and is presented as a systematic process.Finally,the proposed structural design approach is illustrated by two examples to verify its feasibility and practicality.This study provides an effective method for designing a practical multi-mobility and multiconfiguration planar single-loop metamorphic mechanism with a single actuator.Qiang Yang Guangbo Hao Shujun Li Hongguang Wang Haiyang Li 2020Chinese Journal of Mechanical Engineering2020,33,5:5
10800 Gbit/s transmission over 1 km single-mode fiber using a four-channel silicon photonic transmitter显示文摘We demonstrate the optical transmission of an 800 Gbit/s(4×200 Gbit/s)pulse amplitude modulation-4(PAM-4)signal and a 480 Gbit/s(4×120 Gbit/s)on–off-keying(OOK)signal by using a high-bandwidth(BW)silicon photonic(SiP)transmitter with the aid of digital signal processing(DSP).In this transmitter,a four-channel SiP modulator chip is co-packaged with a four-channel driver chip,with a measured 3 dB BW of 40 GHz.DSP is applied in both the transmitter and receiver sides for pre-/post-compensation and bit error rate(BER)calculation.Back-to-back(B2B)BERs of the PAM-4 signal and OOK signal are first measured for each channel of the transmitter with respect to a variety of data rates.Similar BER performance of four channels shows good uniformity of the transmitter between different channels.The BER penalty of the PAM-4 and OOK signals for 500 m and 1 km standard single-mode fiber(SSMF)transmission is then experimentally tested by using one channel of the transmitter.For a 200 Gbit/s PAM-4 signal,the BER is below the hard-decision forward error correction(HD-FEC)threshold for B2B and below the soft-decision FEC(SD-FEC)threshold after 1 km transmission.For a 120 Gbit/s OOK signal,the BER is below SD-FEC threshold for B2B.After 500 m and 1 km transmission,the data rate of the OOK signal shrinks to 119 Gbit/s and 118 Gbit/s with the SD-FEC threshold,respectively.Finally,the 800 Gbit/s PAM-4 signal with 1 km transmission is achieved with the BER of all four channels below the SD-FEC threshold.Hongguang Zhang Miaofeng Li Yuguang Zhang Di Zhang Qiwen Liao Jian He Shenglei Hu Bo Zhang Lei Wang Xi Xiao Nan Qi Shaohua Yu 2020Photonics Research2020,8,11:5
11Lichtenstein inguinal hernia repairs with porcine small intestine submucosa: a 5- year follow-up. a prospective randomized controlled study显示文摘Porcine small intestine submucosa(SIS)biologic patch has been used in inguinal hernia repair.However,there are lttle data available to assess the long-term effect after repair.This study aimed to explore the long-term effect of SIS patch in open inguinal hernia repair.Sevent-six patients with unilateral inguinal hernia were treated with Lichtenstein tension-free hernia repair using SIS patch(Beijing Datsing Bio-Tech Co,Ltd.)and Surgisis patch(COOK,USA)in Tianjn Union Medical Center and China-Japan Friendship Hospital.In the trial,the long-term efficacy of the treatment group and the control group were compared.A total of 66 patients in both groups received long-term follow-up(>5years)after surgery,with a followup rate of 86.8%.During the follow-up period,there was one case of recurrence,one case of chronic pain in the control group.There was no statistically significant difference(P>0.05)in terms of recurrence,chronic pain,foreign body sensation and infection between the two groups of patients.After long-term observations,it has been found that the porcine small intestinal submucosa(SIS)biological patch is safe and effective for inguinal hernia Lichtenstein repair,and has a low recurrence rate and complication rate.Baoshan Li Xin Zhang Yi Man Jiadong Xie Wei Hu Huang Huang Yinlong Wang Hongguang Ma 2021Regenerative Biomaterials2021,8,1:5
12Effects of propofol on early and late cytokines in lipopolysaccharideinduced septic shock in rats显示文摘Objective:It has been reported that the intravenous anesthetic propofol(PPF)has anti-inflammatory effects in vitro and in patients.The purpose of this study was to investigate whether PPF has anti-inflammatory effects in lipopolysaccharide(LPS)-induced septic shock by inhibiting the induction of pro-inflammatory cytokinesinterleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)and high mobility group box 1(HMGB1)in rats.Methods:Thirty six male Wistar rats were randomly assigned to one of three groups(control group,PPF+LPS group and LPS group;n=12 per group).Control group rats received a 0.9%NaCl solution(NS)by the tail vein.The PPF+ LPS group rats received PPF(10 mg/kg bolus,followed by infusion at 10 mg/(kg·h)through a femoral vein catheter)1 h before LPS(7.5 mg/kg)administration via the tail vein.The LPS group rats received injection of LPS(7.5 mg/kg)via the tail vein.Hemodynamic effects were recorded as well as mortality rates,and plasma cytokine concentrations(TNF-α,IL-6,HMGB1)were measured for the 24-h observation period.Results:The mean arterial pressure and heart rate of the PPF+LPS group were more stable than those of the LPS group.The mortality at 24 h after the administration of the LPS injection was much higher in the LPS group(58.3%)compared to the PPF+ LPS group(25.0%).Plasma concentrations of cytokines(IL-6 and TNF-α)and HMGB1 were significantly reduced in the PPF+LPS group compared to the LPS group(P<0.05).Conclusion:Pretreatment with PPF reduced the mortality rate of rats and attenuated the pro-inflammatory cytokine responses in an endotoxin shock model through an anti-inflammatory action inhibiting induction of HMGB1.Sha Li Hongguang Bao Liu Han Lele Liu 2010The Journal of Biomedical Research2010,24,5:4
13Clinical significance of HBME-1,Galectin-3,and CK19 expression and the status of BRAF mutation in papillary thyroid carcinoma显示文摘Objective The aim of this study was to explore the clinical significance of the expression of proteins human bone marrow endothelial cell markers(HBME-1), Galectin-3, and cytokeratin19(CK19), as well as the status of v-raf murine sarcoma viral oncogene homolog B1(BRAF) mutation in papillary thyroid carcinoma(PTC). Methods Immunohistochemical staining was performed in 82 specimens each of PTC and papillary benign lesions to detect the expression of HBME-1, Galectin-3, and CK19. Polymerase chain reaction(PCR) and gene sequencing were performed on 60 specimens each of PTC and papillary benign lesions to detect the status of BRAF mutation. Results The positive expression ratios of HBME-1, Galectin-3, and CK19 in PTC were 98.8%, 97.6% and 100% respectively, which were significantly higher than the expressions in papillary benign lesions(P < 0.05). No significant relationship was observed between the expression of these makers and the clinicopathological features of PTC. The sensitivity of co-expression of HBME-1 and CK19 or HBME-1 and Galectin-3 as diagnostic criteria of PTC was 99.9%, with a specificity of 95.4%. BRAF mutation was detected in 40 of 60 PTC(66.7%) specimens. There was a statistical difference in BRAF mutations between PTC and papillary benign lesions(P < 0.05); there were no associations between BRAF mutation and the clinicopathological features of PTC. Conclusion Combined immunohistochemical staining of HBME-1, Galectin-3, and CK19 can further improve the sensitivity and specificity of differential diagnosis of PTC. BRAF mutation is a significant genetic event, which may have diagnostic value for PTC.Li Zheng Min Zhao Xiangyang Hu Jin Huang Ling Ang Hongguang Hu Qiang Zou Jin Wang Mingqiang Liu Yang Zhao 2016Oncology and Translational Medicine2016,2,4:4
14Enhancing phosphorus uptake efficiency through QTL-based selection for root system architecture in maize显示文摘Root system architecture(RSA) plays an important role in phosphorus(P) acquisition,but enhancing P use efficiency(PUE) in maize via genetic manipulation of RSA has not yet been reported.Here,using a maize recombinant inbred line(RIL) population,we investigated the genetic relationships between PUE and RSA,and developed P-efficient lines by selection of quantitative trait loci(QTLs) that coincide for both traits.In low-P(LP) fields,P uptake efficiency(Pup E) was more closely correlated with PUE(r =0.48-0.54),and RSA in hydroponics was significantly related to Pup E(r=0.25-0.30) but not to P utilization efficiency(Put E).QTL analysis detected a chromosome region where two QTLs for PUE,three for Pup E and three for RSA were assigned into two QTL clusters,Cl-bin3.04 a and Cl-bin3.04 b.These QTLs had favorable effects from alleles derived from the large-rooted and high-Pup E parent.Marker-assisted selection(MAS) identified nine advanced backcross-derived lines carrying Cl-bin3.04 a or Cl-bin3.04 b that displayed mean increases of 22%e26% in PUE in LP fields.Furthermore,a line L224 pyramiding Clbin3.04 a and Cl-bin3.04 b showed enhanced Pup E,relying mainly on changes in root morphology,rather than root physiology,under both hydroponic and field conditions.These results highlight the physiological and genetic contributions of RSA to maize Pup E,and provide a successful study case of developing P-efficient crops through QTL-based selection.Riliang Gu Fanjun Chen Lizhi Long Hongguang Cai Zhigang Liu Jiabo Yang Lifeng Wang Huiyong Li Junhui Li Wenxin Liu Guohua Mi Fusuo Zhang Lixing Yuan 2016Journal of Genetics and Genomics2016,43,11:3
15A review of recent progress in preparation of hollow polymer microspheres显示文摘The preparation methods of hollow polymer microspheres both at home and abroad are summarized,and their preparation mechanisms and developmental states are presented.These methods include the liquid droplet method,dried-gel droplet method,self-assembly method,microencapsulation method,emulsion polymerization method and the template method.Hollow polystyrene microspheres are the most extensively studied in the research of hollow polymer microspheres.Through comparison of the advantages and disadvantages of different preparation methods,it is concluded that microencapsulation method is most suitable for preparing polystyrene hollow microspheres.Wei Bin Wang Shujun Song Hongguang Liu Hongyan Li Jie Liu Ning 2009Petroleum Science2009,6,3:3
16Insight into the role of binding interaction in the transformation of tetracycline and toxicity distribution显示文摘The transformation of free state organic micro-pollutants(MPs)has been widely studied;however,few studies have focused on mixed and bound states MPs,even though numerous ionizable organic MPs process a strong tendency to combine with dissolved organic matters in aquatic environments.This study systemically investigated the distribution and toxicity assessment of tetracycline(TET)transformation products in free,mixed and bound states during UV,UV/H2O2,UV/PS and CNTs/PS processes.A total of 33 major transformation products were identified by UPLC-Q-TOF-MSMS analysis,combining the double bond equivalence and aromaticity index calculations.The binding interaction would weaken the attack on the dimethylamino(-N(CH3)2)group and induce the direct destruction of rings A and B of TET through the analysis of 2D Kernel Density changes and density functional theory(DFT)calculations.Toxicity assessment and statistics revealed that the intermediate products with medium molecular weight(230m/z380)exhibited higher toxicity,which was closely related to the number of the rings in molecular structures(followed as 2»3>1z4).A predicted toxicity accumulation model(PTAM)was established to evaluate the overall toxicity changes during various oxidation processes.This finding provides new insight into the fate of bound MPs during various oxidation processes in the natural water matrix.Bo Yang Xin Cheng Yongli Zhang Wei Li Jingquan Wang Hongguang Guo 2021Environmental Science and Ecotechnology2021,,4:3
17Energy measurement system of a large-aperture high power laser experiment platform显示文摘An energy measurement system in a large-aperture high power laser experiment platform is introduced. The entire measurement system includes five calorimeters, which carry out the energy measurement of the fundamental frequency before the frequency conversion unit, remaining fundamental frequency, remaining second-harmonics, third-harmonics,as well as the energy balance measurement after the frequency conversion unit. Combinational indirect calibration and direct calibration are employed to calibrate the sampling coefficients of the calorimeters. The analysis of the data showed that, regarding the energy balance coefficients, combinational calibration approach gives a higher precision, and leads to an energy balance with 1%; and regarding the energy sampling coefficients for the various wavelengths after the frequency conversion, the results from direct and combinational calibration are consistent. The uncertainties for all energy sampling coefficients are within 3%, which guarantees the reliability of the energy measurement for the laser facility.Yanwen Xia Yue Liang Sen Li Junpu Zhao Zhitao Peng Hongguang Li Hua Liu Zhihong Sun Kuixing Zheng Xiaofeng Wei 2013High Power Laser Science and Engineering2013,1,Z1:2
18High-speed and high-power germanium photodetector with a lateral silicon nitride waveguide显示文摘Up to now, the light coupling schemes of germanium-on-silicon photodetectors(Ge-on-Si PDs) could be divided into three main categories:(1) vertical(or normal-incidence) illumination, which can be from the top or back of the wafer/chip, and waveguide-integrated coupling including(2) butt coupling and(3) evanescent coupling. In evanescent coupling the input waveguide can be positioned on top, at the bottom, or lateral to the absorber. Here,to the best of our knowledge, we propose the first concept of Ge-on-Si PD with double lateral silicon nitride(Si_(3)N_(4)) waveguides, which can serve as a novel waveguide-integrated coupling configuration: double lateral coupling. The Ge-on-Si PD with double lateral Si_(3)N_(4) waveguides features uniform optical field distribution in the Ge region, which is very beneficial to improving the operation speed for high input power. The proposed Ge-on-Si PD is comprehensively characterized by static and dynamic measurements. The typical internal responsivity is evaluated to be 0.52 A/W at an input power of 25 mW. The equivalent circuit model and theoretical 3 dB optoelectrical(OE) bandwidth investigation of Ge-on-Si PD with lateral coupling are implemented. Based on the small-signal(S21) radio-frequency measurements, under 4 mA photocurrent, a 60 GHz bandwidth operating at-3 V bias voltage is demonstrated. When the photocurrent is up to 12 mA, the 3 dB OE bandwidth still has 36 GHz. With 1 mA photocurrent, the 70, 80, 90, and 100 Gbit/s non-return-to-zero(NRZ) and 100,120, 140, and 150 Gbit/s four-level pulse amplitude modulation clear openings of eye diagrams are experimentally obtained without utilizing any offline digital signal processing at the receiver side. In order to verify the highpower handling performance in high-speed data transmission, we investigate the eye diagram variations with the increase of photocurrents. The clear open electrical eye diagrams of 60 Gbit/s NRZ under 20 mA photocurrent are also obtained. Overall, the proposed lateral Si_(3)N_(4) waveguide structure is flexibly extendable to a light coupling configuration of PDs, which makes it very attractive for developing high-performance silicon photonic integrated circuits in the future.XIAO HU DINGYI WU HONGGUANG ZHANG WEIZHONG LI DAIGAO CHEN LEI WANG XI XIAO SHAOHUA YU 2021Photonics Research2021,9,5:2
19SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants显示文摘RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals.However,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in plants.Here,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide.Pelota enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all potyvirids.This allows Pelota to target the virus and act as a viral restriction factor.Furthermore,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta.Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation.These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.Linhao Ge Buwei Cao Rui Qiao Hongguang Cui Shaofang Li Hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li 2023Molecular Plant2023,16,3:2
20An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya显示文摘Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced.However,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species.Here,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia coli.The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps.Using this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate biosynthesis.The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya.Decai Tuo Pu Yan Guangyuan Zhao Hongguang Cui Guopeng Zhu Yang Liu Xiukun Yang He Wang Xiaoying Li Wentao Shen Peng Zhou 2021Horticulture Research2021,8,1:2
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