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| 1 | An environmental flow assessment method based on the relationships between flow and ecological response: A case study of the Three Gorges Reservoir and its downstream reach显示文摘The current environmental flow assessment of Chinese rivers is thought to have three shortcomings: The first is that environmental flow requirements of reservoirs in dammed rivers are usually not explicitly considered; the second is that enough attentions have not been paid to the inherent links between flow regime and ecological processes; the third is that most studies focus on the variable range of merely one hydrological element such as discharge needed by riverine ecosystems. Here, first proposed is a holistic method for environmental flow assessment, the flow-ecological response relationship method that is suitable for large rivers with relatively abundant ecological data. Based on the conceptual models and quantitative relationships between flow and ecological response, this method comprehensively considers the ecological conservation requirements of both reservoir and its downstream reach. Then, it is applied to assessing the environmental flows of the Three Gorges Reservoir and its downstream reach by the following steps: 1) Construction of conceptual models of flow-ecological response; 2) identification of ecological targets of environmental flows and their key periods; 3) development of the quantitative relationships between hydrological indicators and ecological indictors; 4) preliminary assessment of environmental flow according to the tradeoff between ecological targets and water demands of human. The environmental flow hydrographs obtained have explicit ecological conservation targets, time schedule of achieving each target, and characteristics of multiple hydrological elements such as flow, water level, frequency, timing, duration and rate of change. The case study has tested the reasonability and feasibility of this method, and the results of this study are expected to provide technical support and decision reference for improving the operation of the Three Gorges-Gezhouba cascade reservoirs. | WANG JunNa DONG ZheRen LIAO WenGen LI Chong FENG ShunXin LUO HuiHuang PENG QiDong | 2013 | Science China(Technological Sciences)2013，56，6： | 16 |
| 2 | Generation and characterization of monoclonal antibodies against the NS1 protein of H17N10 bat influenza virus显示文摘 | Junna Gao Kaidi Xu Haiwei Sun Qinfang Liu Jianmei Yang Qiaoyang Teng Xuesong Li Zhaoguo Chen Zejun Li Hongjun Chen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，7： | 2 |
| 3 | Synthesis of Ag-SiO_2 composite nanospheres and their catalytic activity显示文摘A simple,mild,and time-saving method is employed to synthesize Ag-SiO2 composite nanospheres with Ag nanoparticles uniformly distributed on the surface of SiO2 nanoparticles.The chemical elements and the morphology of Ag-SiO2 composite nanospheres were analyzed with transmission electron microscopy(TEM),X-ray power diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).On the surface of Ag-SiO2 composite nanospheres,silane coupling agent(KH-550)is introduced as an intermediary to connect the surfaces of SiO2 nanospheres and Ag nanoparticles,which is also helpful for avoiding the aggregation of Ag nanoparticles.It is found that Ag-SiO2 composite nanospheres have very good catalytic properties for the reduction of organic dyes,which may have potential application in wastewater treatment. | HAN QingYan LI GuiAn WANG DeXuan HE EnJie DONG Jun GAO Wei LI JunNa LIU TingZhuo ZHANG ZhongYue ZHENG HaiRong | 2014 | Science China Chemistry2014，57，6： | 2 |
| 4 | Transcriptomic Analysis of the Tolerance Response to Dehydration and Rehydration in Wheat Seedlings显示文摘Drought is the main abiotic stress that restricts wheat production.The rapid development of sequencing technology and its widespread application to various fields have revealed the structural characteristics and regulation of related genes through gene expression analysis.Here,we studied responses of wheat plants under drought and rewatering conditions,using morphological and physiological indicators.Moreover,a transcriptome analysis was conducted on Jingmai 12,a drought-resistant wheat strain,to explore the mechanism underlying the response of drought-resistant wheat seedlings to drought stress at the transcriptome level.Drought stress caused morphological and physiological changes in both drought-resistant and-sensitive varieties,but to a greater extent in the drought-sensitive specimen.After re-watering,the drought-resistant wheat showed greater ability to recover than the drought-sensitive wheat.Transcriptome sequencing of Jingmai 12 revealed 97,422 genes,including 80,373 known genes and 17,049 newly predicted genes.The observed upregulation of genes was mostly involved in hormone and signal transduction,carbon metabolism,amino acid synthesis,small molecule production,transmembrane transport,ROS detoxification and defense,drought response protein,and protective enzyme activity.Downregulated genes were mostly involved in photosynthesis,lipid metabolism,signaling,and auxin response.Upon rehydration,these genes and metabolic pathways returned to normal.Our results suggest that all these changes are adaptations to drought stress.Through morphological adaptation,physiological regulation,and the expression of drought-induced genes,normal growth of drought-resistant varieties under drought stress can be promoted.These results increase our understanding of the transcriptomic changes taking place in drought-resistant wheat seedlings under drought stress,and provide a direction for future investigations. | Ping Zhang Zhiyou Kong Junna Liu Yongjiang Liu Qianchao Wang Xiuju Huan Li Li Yunfeng Jiang Peng Qin | 2022 | Phyton-International Journal of Experimental Botany2022，91，2： | 1 |
| 5 | Anticoagulation for the treatment of portal vein thrombosis in liver cirrhosis: A systematic review and meta-analysis of observational studies显示文摘 | Xingshun Qi Valerio De Stefano Hongyu Li Junna Dai Xiaozhong Guo Daiming Fan | 2014 | European Journal of Internal Medicine2014，，： | 1 |
| 6 | Catalyt- ic ozonation of bisphenol A in aqueous solution using Mn-Ce/HZSM-5 as catalyst显示文摘 | Liu Yanfang Zhao Junna Li Zaixing | 2015 | Water Sci Technol2015，72，5： | 1 |
| 7 | Spatiotemporal variation of agricultural patterns in different geomorphologic and climatic environments in the eastern Loess Plateau,north-central China during the late Neolithic and Bronze Ages显示文摘Human settlement and agricultural development are closely linked to local geomorphological and climatic environments.However,the variation in agricultural systems in different environmental and prehistoric contexts remains unknown.We report new archaeobotanical and radiocarbon dates from 34 Neolithic and Bronze Age sites in the Hutuo River Valley(HTRV)in north-central China and compare them with updated archaeobotanical studies in the Sushui River Valley(SSRV)and Henan Province(HNP),to explore the similarities and differences of agricultural patterns under different geomorphologic and climatic environments in north-central China.Our results reveal that humans consistently cultivated foxtail and broomcorn millet in the HTRV from the Miaodigou(6000–5700 cal.a BP)to the Shang-Zhou(3600–2256 cal.a BP)period,despite the introduction of wheat and barley into the area around 4000 a BP.Climate conditions and hilly landforms in the HTRV,SSRV and some parts of the HNP led people to develop foxtail and broomcorn millet dry land farming practices between 7000 and 3000 a BP.Alternatively,in other areas of the HNP,the climate conditions and alluvial plains enabled people to develop a mixed agriculture of millets,soybeans,and rice from 7000 to 4000 a BP,with the addition of wheat between 4000 and 3000 a BP.Farmers’different agricultural technologies and interactions with foreign cultures may have also influenced the formation of different agricultural patterns in the three regions between 7000 and 3000 a BP.Population growth during 7000–3000 a BP can explain the overall propensity of the higher-yield foxtail millet rather than broomcorn millet as the main cultivated crop.In the HTRV,however,higher proportions of broomcorn millet(as compared to the SSRV and HNP),may reflect the greater drought and heat tolerance of broomcorn millet,representing an adaptive agricultural strategy in the river valley. | Haiming LI Yifu CUI Nathaniel JAMES Melissa RITCHEY Fengwen LIU Junna ZHANG Minmin MA Guanghui DONG | 2022 | Science China Earth Sciences2022，65，5： | 0 |
| 8 | The role of CDK1 siRNA interference in cell cycle and cell apoptosis显示文摘In the present report,cyclin-dependent kinase1(CDK1)siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis.The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000.The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction(PCR)and Western blot,respectively.The cell cycle was analyzed by using DNA content analysis byflow cytometry.Cell apoptosis was detected by the Annexin V/PI method.The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain.CDK1 gene was successfully silenced by its siRNA,and the CDK1 protein expression level was decreased significantly,especially from 48th h to 60th h after transfection.The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G2/M phase.There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60 h.More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells.The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G2/M phase but did not induce apoptosis. | Hui XIAO Ming TIAN Junna GE Xin WEI Zhaoming LI Xiaolan LI Deding TAO Junbo HU Jianping GONG | 2009 | Frontiers of Medicine2009，3，4： | 0 |
| 9 | R2R3-type Lo MYB21 affects jasmonate-regulated development and dehiscence of anthers in lily(Lilium oriental hybrids)显示文摘Lilies are widely cultivated for cut flowers,but their large anthers carry a considerable amount of colored pollen that is dispersed easily.Studying the molecular mechanism of anther development and dehiscence could help solve this problem.LoMYB21,encoding a putative R2R3v-myb avian myeloblastosis viral oncogene homolog(MYB)transcription factor,was identified from oriental lilies(Lilium‘Siberia’).Real-time quantitative PCR analysis showed that LoMYB21 was mainly expressed in the anther,filament and stigma and had high expression during the late stages of lily anther development.LoMYB21 had transactivation activity and was located in the nucleus through yeast one-hybrid assays and transient expression in Nicotiana benthamiana.Suppression of LoMYB21 by virus-induced gene silencing(VIGS)in Lilium‘Siberia’led to anther indehiscence and reduced the expression of genes related to Jasmonate acid(JA)biosynthesis and signal transduction.Induction of LoMYB21 in DEX::LoMYB21 transgenic Arabidopsis caused procumbent inflorescences that became infertile,accompanied by higher expression of JA biosynthetic and signaling genes.These results demonstrated that JA content and signaling were abnormal in silenced lily and transgenic LoMYB21 Arabidopsis,which affected anther development.Our study indicated that LoMYB21 could regulate lily anther dehiscence through JA biosynthesis and signaling during the late stages of anther development. | Zheng Tong Tingting Dong Qiuhua Li Rui Wang Junna He Bo Hong | 2024 | Horticultural Plant Journal2024，10，1： | 0 |
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