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| 1 | Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity. | YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China | 2007 | Science China(Life Sciences)2007，50，2： | 4 |
| 2 | The characterization of auditory brainstem response(ABR) waveforms: A study in tree shrews(Tupaia belangeri)显示文摘To characterize the patterns of ABR waves in tree shrews, we must understand the hearing sensitivity and auditory function of healthy adult tree shrews. Fifteen tree shrews(30 ears) were stimulated with clicks and tone-pips at 11 different frequencies from 1 to 60 kHz. The ABR waves were recorded and analyzed. The ABR consisted of five to seven positive waves in the first 10 ms after a click stimulus, and the average hearing threshold of component III was 27.86 ± 3.78 dB SPL. Wave III was the largest and most clear. The ABR threshold was related to the tone-pip sitmulus by a 'U' shaped curve. The sensitive frequency was approximately 8 kHz in tree shrews. The latencies systematically decreased with increasing stimulus frequencies. The ABR amplitudes of wave III increased as the sound pressure level increased. All of these results provide an empirical basis for future studies of hearing diseases in tree shrews. | Lihong Xie Menglin Wang Ting Liao Songhua Tan Kai Sun Heng Li Qin Fang Anzhou Tang | 2018 | Journal of Otology2018，13，3： | 4 |
| 3 | Marsdenia tenacissima injection induces the apoptosis of prostate cancer by regulating the AKT/GSK3β/STAT3 signaling axis显示文摘Marsdenia tenacissima injection,a standard Marsdenia tenacissima extract(MTE),has been approved as an adjuvant therapeutic agent for various cancers.Our previous study showed that MTE inhibited the proliferation and metastasis of prostate cancer(PCa)cells.However,the underlying mechanisms and active ingredients of MTE against PCa were not completely understood.This study revealed that MTE induced significant decreases in cell viability and clonal growth in PCa cells.In addition,MTE induced the apoptosis of DU145 cells by reducing the mitochondrial membrane potential and increasing the expression of Cleaved Caspase 3/7,Cyt c,and Bax.In vivo,DU145 xenografted NOD-SCID mice treated with MTE showed significantly decreased tumor size.TUNEL staining and Western blot confirmed the pro-apoptotic effects of MTE.Network pharmacology analysis collected 196 ingredients of MTE linked to 655 potential targets,and 709 PCa-associated targets were retrieved,from which 149 overlapped targets were screened out.Pathway enrichment analysis showed that the HIF-1,PI3K-AKT,and ErbB signaling pathways were closely related to tumor apoptosis.Western blot results confirmed that MTE increased the expression of p-AKT^(Ser473) and p-GSK3β^(Ser9),and decreased the expression of p-STAT3^(Tyr705) in vitro and in vivo.A total of 13 compounds in MTE were identified by HPLC-CAD-QTOF-MS/MS and UPLCQTOF-MS/MS.Molecular docking analysis indicated that six compounds may interact with AKT,GSK3β,and STAT3.In conclusion,MTE induces the endogenous mitochondrial apoptosis of PCa by regulating the AKT/GSK3β/STAT3 signaling axis,resulting in inhibition of PCa growth in vitro and in vivo. | LI Xiaolan HE Songhua LIANG Wei ZHANG Weiquan CHEN Xin LI Qiaofeng YANG Xin LIU Yanying ZHU Dan LI Li LIU Buming SU Zhiheng CHEN Jie GUO Hongwei | 2023 | Chinese Journal of Natural Medicines2023，21，2： | 2 |
| 4 | Active anticorrosion and self-healing coatings:A review with focus on multi-action smart coating strategies显示文摘The development of smart coatings with potential for active anticorrosion and self-healing protection of metals is essential for long-term performance of metallic structures in aggressive chemical environments.Presently,emphasis has been placed on the development of advanced smart coatings for corrosion protection in different applications.Innovative multifunctional coatings with fascinating stimuliresponsive functionalities are considered“smart”.The stimuli-responsive functionalities of these smart coatings when properly harnessed result in a class of coatings with inherent autonomous control of corrosion.Fundamentally,when metals are exposed to aggressive environments,occurrences at the metalsolution interface cause environmental changes.These changes can be controlled when triggers from external environment set off active components of smart coating,thereby enhancing coating’s life and functionality.Common triggers include the availability of moisture,concentration of chloride ion,p H gradient,mechanical damage,impact,fatigue,light,redox activity and temperature.In this review,recent technological trends in active anticorrosion and self-healing coatings as functional routes for metal protection are summarized,stimuli responsiveness and mechanisms of inhibition are discussed,and recent multi-action protective systems are particularly focused on. | Inime Ime Udoh Hongwei Shi Enobong Felix Daniel Jianyang Li Songhua Gu Fuchun Liu En-Hou Han | 2022 | Journal of Materials Science & Technology2022，，21： | 2 |
| 5 | Fuzzy models for analysis of rock mass displacements due to underground mining in mountainous areas显示文摘 | Wen-Xiu Li Songhua Mei Shuhua Z | 2006 | International Journal of Rock Mechanics & Mining Sciences2006，43，4： | 1 |
| 6 | Synthesis and properties of CrN x /amorphous-WC nanocomposites prepared using hybrid arc ion plating and direct current magnetron sputtering显示文摘 | Shihong Zhang Mingxi Li Yizhu He Tongyul Cho Huigon Chun Jaehong Yoon Songhua Si Huisheng Li | 2010 | Thin Solid Films2010，，2： | 1 |
| 7 | Research on the high-speed precision grinding of non-circular parts based on PMAC显示文摘 | WU Yuhou ZHANG Ke LI Songhua | 2004 | Key Engineering Materials2004，259，： | 1 |
| 8 | The combination of carotid and lower extremity ultrasonography increases the detection of atherosclerosis in type 2 diabetes patients显示文摘 | Lianxi Li Haoyong Yu Jiaan Zhu Xing Wu Fang Liu Feng Zhang Qing Li Songhua Wu Yuqian Bao Weiping Jia | 2012 | Journal of Diabetes and Its Complications2012，，1： | 1 |
| 9 | Effects of acute low temperature stress on the endocrine reactions of the Qinghai toad-headed lizard显示文摘 | Chunwang LI Yuan GU Songhua TANG Hongxia FANG Guohua JIANG Zhigang JIANG | 2011 | Current Zoology2011，57，6： | 1 |
| 10 | Research on High-speed precise Grinding of Non-circular parts Based on PMAC显示文摘 | WU YUHOU ZHANG KE LI SONGHUA | 2004 | KEY ENG MAT V2004，，258259： | 1 |
| 11 | Research on High-speed plecise Grinding of Non-circular parts Based on PMAC显示文摘 | Wu Yuhou Zhang Ke Li Songhua | 2004 | Key Engineering Materia2004，，： | 1 |
| 12 | Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of an extract made from Rhizoma Atractylodis Macrocephalae (RAM)显示文摘 | Ruili Li Kedsirin Sakwiwatkul Li Yutao Songhua Hu | 2009 | Vaccine2009，，15： | 1 |
| 13 | A Novel Soft-Switching Full-Bridge PWM DC/DC Converter With Auxiliary Resonant Commutating Network 显示文摘 | Zhu Lizhi Qu Wenlong Li Fahai Shen Songhua | 1996 | IEEE1996，，： | 1 |
| 14 | Overexpression of SLC7A7 predicts poor progression-free and overall survival in patients with glioblastoma显示文摘 | Songhua Fan Delong Meng Tao Xu Yuanyuan Chen Jingkun Wang Xiaoying Li Hongyan Chen Daru Lu Juxiang Chen Qing Lan | 2013 | Medical Oncology2013，，1： | 1 |
| 15 | Research on High-speed precise Grinding of Non-circular parts Based on PMAC显示文摘 | Wu Yuhou Zhang Ke Li Songhua | 2004 | Key Engineering Materials2004，，258259： | 1 |
| 16 | Lapping Machining of High-Speed and High-Precision Ceramic Bearing Balls显示文摘 | Wu Yuhou Li Songhua Zhang Ke | 2005 | Key Engineering Materials2005，，291292： | 1 |
| 17 | Development and characterization of polymorphic microsatellite markers in Linum usitatissimum显示文摘 | Xin Deng Songhua Long Dongfeng He Xiang Li Yufu Wang Jia Liu Xinbo Chen | 2010 | Journal of Plant Research2010，，1： | 1 |
| 18 | Schur Convexity for Two Classes of Symmetric Functions and Their Applications显示文摘For x =(x1, x2, ···, xn) ∈ Rn+∪ Rn-, the symmetric functions Fn(x, r) and Gn(x, r) are defined by r1 + xFij n(x, r) = Fn(x1, x2, ···, xn; r) =x1≤iij1 | Mingbao SUN Nanbo CHEN Songhua LI Yinghui ZHANG | 2014 | Chinese Annals of Mathematics,Series B2014，35，6： | 1 |
| 19 | Fuzzy models for a-nalysis of rock mass displacements due to underground miningin mountainous areas显示文摘 | Li Wenxiu Mei Songhua Zai Shuhua etc | 2006 | International Journal of Rock Me-chanics&Mining Sciences2006，，43： | 1 |
| 20 | Spatial Distribution Pattern of the Steppe Toad-headed Lizard(Phrynocephalus frontalis) and Its Influencing Factors显示文摘Spatial distribution patterns are associated with life history and behavioral adaptations of animals. For studying the spatial distribution pattern of the steppe toad-headed lizard(Phrynocephalus frontalis) and its influencing factors,we conducted experiments in Hunshandake Sandy Land in Inner Mongolia,China in July 2009. By calculating the clustered indices,we found that the lizard was aggregately distributed when the sampling quadrat was smaller than 10 m × 10 m,and uniformly distributed when it was greater than 10 m × 10 m. The Nearest Neighbor Rule showed a clustering distribution pattern for P. frontalis and the distribution pattern was quadrat-sampling dependent. Furthermore,the cluster was determined by environmental factors when the sampling quadrat was smaller than 20 m × 20 m,but it was determined by both environmental factors and characteristics of the lizard when it was larger than 20 m × 20 m. Our results suggested that the steppe toad-headed lizards tended to aggregate into suitable habitat patches in desert areas. Additionally,we discussed that the lizard aggregation could be potentially used as an indictor of movement of sand dunes. | Xue LIAN Zhigang JIANG Xiaoge PING Songhua TANG Junhuai BI Chunwang LI | 2012 | Asian Herpetological Research2012，3，1： | 1 |
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