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| 1 | Systems metabolic engineering strategies for the production of amino acids显示文摘Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary engineering.It is an efficient approach for strain improvement and process optimization,and has been successfully applied in the microbial production of various chemicals including amino acids.In this review,systems metabolic engineering strategies including pathwayfocused approaches,systems biology-based approaches,evolutionary approaches and their applications in two major amino acid producing microorganisms:Corynebacterium glutamicum and Escherichia coli,are summarized. | Qian Ma Quanwei Zhang Qingyang Xu Chenglin Zhang Yanjun Li Xiaoguang Fan Xixian Xie Ning Chen | 2017 | Synthetic and Systems Biotechnology2017，2，2： | 10 |
| 2 | Potential Benefits of Biochar in Agricultural Soils:A Review显示文摘Soil degradation,characterized by declines in nutrient status and simultaneous accumulation of pesticide residues,is a major problem affecting agricultural ecosystems.Previous studies indicate that biochar application to soil has promise as a practical method to alleviate these pressures:increasing crop yield and enhancing pesticide degradation.Here,we review the roles of biochar in both chemical and biological promotion of pesticide degradation and the potential benefits of biochar relating to the efficiency of fertilizer use,availability of nutrients,and nutrient exchange.Biochar typically has a high surface area featuring many functional groups,a high cation exchange capacity,and high stability.Major factors that impact on the nutrient retention characteristics of biochar(e.g.,feedstock,pyrolysis temperature,and application rate) are also discussed herein.Nonetheless,more studies of the long-term impacts on soil properties from biochar addition are still required before it can be possible to accurately quantify the sustainability of this approach to sequester carbon and restore soil function. | DING Yang LIU Yunguo LIU Shaobo HUANG Xixian LI Zhongwu TAN Xiaofei ZENG Guangming ZHOU Lu | 2017 | Pedosphere2017，27，4： | 10 |
| 3 | Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube显示文摘Identification,sorting,and sequencing of individual cells directly from in situ samples have great potential for in-depth analysis of the structure and function of microbiomes.In this work,based on an artificial intelligence(AI)-assisted object detection model for cell phenotype screening and a cross-interface contact method for single-cell exporting,we developed an automatic and index-based system called EasySort AUTO,where individual microbial cells are sorted and then packaged in a microdroplet and automatically exported in a precisely indexed,“One-Cell-One-Tube”manner.The target cell is automatically identified based on an AI-assisted object detection model and then mobilized via an optical tweezer for sorting.Then,a crossinterface contact microfluidic printing method that we developed enables the automated transfer of cells from the chip to the tube,which leads to coupling with subsequent single-cell culture or sequencing.The efficiency of the system for single-cell printing is>93%.The throughput of the system for single-cell printing is~120 cells/h.Moreover,>80%of single cells of both yeast and Escherichia coli are culturable,suggesting the superior preservation of cell viability during sorting.Finally,AI-assisted object detection supports automated sorting of target cells with high accuracy from mixed yeast samples,which was validated by downstream single-cell proliferation assays.The automation,index maintenance,and vitality preservation of EasySort AUTO suggest its excellent application potential for single-cell sorting. | Zhidian Diao Lingyan Kan Yilong Zhao Jingyun Song Chen Wang Yang Liu Fengli Zhang Teng Xu Rongze Chen Xixian Wang Xiaoyan Jing Jian Xu Yuandong Li Bo Ma | 2022 | mLife2022，1，4： | 2 |
| 4 | Supporting Early Pruning in Top-k Query Processing on Massive Data显示文摘 | HAN Xixian LI Jianzhong YANG Donghua | 2011 | Infor- mation Processing Letters2011，111，11： | 1 |
| 5 | Gene selection using hybrid particle swarm optimization and genetic algorithm显示文摘 | Shutao Li Xixian Wu Mingkui Tan | 2008 | Soft Computing2008，，11： | 1 |
| 6 | Base-free oxidative esterification of 5-hydroxymethylfurfural to furan-2,5-dimethylcarboxylate over n-doped carbon-supported Co/Fe bimetallic catalyst under batch-operation or continuous-flow conditions显示文摘Developing an efficient and easily available catalyst for the selective conversion of biomass-derived 5-hydroxymethylfurfural(HMF)into furan-2,5-dimethylcarboxylate(FDMC),a valuable biomass-based monomer,remains a high demand but formidable challenge.Herein,a facile strategy for the synthesis of N-doped carbon-supported Co/Fe bimetallic catalyst(CoFe-NC)was developed,which provided an outstanding FDMC yield of 93%in a batch reactor(base-free,80℃,2 bar O_(2),4 h).Interestingly,CoFe-NC also gave a high FDMC yield of 91%under continuous-flow conditions for 80 h(5 bar O_(2),80℃,GHSV 1320 h^(-1),LHSV 0.6 h^(-1),base-free).Notably,it is the first time that a non-noble catalyst gave such a high FDMC yield under continuous-flow conditions.The introduction of Fe could greatly improve both the electron intensity of Co-N_(x)species and basicity of the catalyst,which endowed CoFe-NC with improved O_(2)activation capacity and enhanced dehydrogenation activity for the oxidation-esterification of HMF.This work delineates the efficient strategy on the construction of N-doped carbon-supported non-noble catalyst,which might open a new avenue for developing highly efficient catalyst for FDMC production. | Weizhen Xie Binglin Chen Wenlong Jia Huai Liu Zheng Li Shuliang Yang Xing Tang Xianhai Zeng Yong Sun Xixian Ke Tianyuan Li Huayu Fang Lu Lin | 2022 | Journal of Energy Chemistry2022，，12： | 1 |
| 7 | Gene selection using hybrid particle swarm optimization and genetic algorithm 显示文摘 | Li Shutao Wu Xixian Tan Mingkui | 2008 | Soft Comput2008，12，： | 1 |
| 8 | Honeysuckle wait for medicine make fungus preliminary observation of function 显示文摘 | Li Xixian | 1995 | China'' s Medical Magazine1995，41，10： | 1 |
| 9 | Gene Selection Using Hybrid Particle Swarm Optimization and Genetic Algo- rithm 显示文摘 | Li Shutao Wu Xixian Tan Mingkui | 2008 | Soft Computing2008，12，11： | 1 |
| 10 | Mechanistic insight into catalytic oxidation of cyclohexane over carbon nanotubes: kinetics and in-situ spectroscopic evidences 显示文摘 | Yang Xixian Wang Hong=juan Li Jing Zheng Wenxu Xiang Rong Tang Zikang Yu Hao Peng Feng | 2013 | Chemistry A European Journal2013，19，30： | 1 |
| 11 | Gene Selection Using Hybrid Particle Swarm Optimization and Genetic Algorithm显示文摘 | Li Shutao Wu Xixian Tan Mingkui | 2008 | Soft Computing2008，12，11： | 1 |
| 12 | Gene Selection Using Genetic Algorithm and Support vectors machines 显示文摘 | Shutao li Xixian Wu Mingkui Tan | 2008 | Soft computing2008，12，： | 1 |
| 13 | Gene Selection Using Genetic Algorithm and Support Vectors Machines显示文摘 | Li Shutao Wu Xixian Hu Xiaoyan | 2008 | Soft Computing2008，12，7： | 1 |
| 14 | Gene selection using hybrid particle swarm optimization and genetic algorithm显示文摘 | LI Shutao WU Xixian TAN Mingkui | 2008 | Soft Computing2008，12，11： | 1 |
| 15 | Flux Pinning in Y_(1-x)M_(x)Ba_(2)Cu_(3)O_(7-δ)(M=Gd,Eu and Sm)System显示文摘In this paper,the flux pinning effects in Y_(1-x)M_(x)Ba_(2)Cu_(3)O_(7-δ)(M=Gd,Eu and Sm)samples are reported.It is found that there is another pinning mechanism under higher magnetic fields in addition to that under lower fields.This pinning effect may stem from the lattice stress field resulting from the partial substitution of the rare earth elements with different atomic radii for Y. | LI Jonghen FANG Minghu HU Gangjin ZHANG Xiaojun JIAO Zhengkuan ZHANG Qirui ZHANG Yitong JIN Xin YAO Xixian | 1992 | Chinese Physics Letters1992，9，12： | 0 |
| 16 | Catalyst design strategy toward the efficient heterogeneously-catalyzed selective oxidation of 5-hydroxymethylfurfural显示文摘The selective oxidation of 5-hydroxymethylfurfural(HMF),a versatile bio-based platform molecule,leads to the formation of several intriguing and useful downstream chemicals,such as 2,5-diformylfuran(DFF),5-hydroxymethyl-2-furancarboxylic acid(HMFCA),formyl 2-furancarboxylic acid(FFCA),2,5-furandicarboxylic acid(FDCA) and furan-2,5-dimethylcarboxylate(FDMC).These products have been extensively employed to fabricate novel polymers,pharmaceuticals,sustainable dyes and many other value-added fine chemicals.The heart of the developed HMF oxidation processes is always the catalyst.In this regard,this review comprehensively summarized the established heterogeneous catalyst design strategy for the selective oxidation of HMF via thermo-catalysis.Particular attention has been focused on the reaction mechanism of HMF oxidation over different catalysts as well as enhancing the catalytic performance of the catalyst through manipulating the properties of the support and fabricating of multi-component metal nano-particles and oxides.The current challenges and possible research directions for the catalytic oxidation of HMF in the future are also discussed. | Huai Liu Xing Tang Xianhai Zeng Yong Sun Xixian Ke Tianyuan Li Jiaren Zhang Lu Lin | 2022 | Green Energy & Environment2022，7，5： | 0 |
| 17 | Synthesis of a new iron(Ⅲ) porphyrin acrylate-styrene copolymer and its catalysis for hydroxylation of cyclohexane显示文摘A new iron(III)porphyrin acrylate-styrene copo-lymer,P[(PorFe)A-S],was synthesized by the reaction of iron(III)porphyrin acrylate with styrene and characterized by UV-Vis,Infrared spectra(IR),inductively coupled plasma-atomic emission spectrometry(ICP)and molecular weight determination.Its catalytic activity in the hydroxylation of cyclohexane for model cytochrome P450 in the P[(PorFe)A-S]-O_(2)–ascrobate-thiosalicylic acid system has been studied.It was found that the P[(PorFe)A-S]has a higher catalytic activity than non-supported iron(III)porphyrin and its high catalytic activity remained in reuse.The catalytic activity of P[(PorFe)A-S]was discussed in the view of the microenvi-ronment of iron(III)porphyrin.It is proposed that the cata-lytic activity of the P[(PorFe)A-S]may be further enhanced by construction of a homophase catalytic system containing the iron(III)porphyrin acrylate-styrene copolymer. | YU Hancheng CHEN Xianli LI Xixian HUANG Jinwang JI Liangnian | 2007 | Frontiers of Chemical Science and Engineering2007，1，1： | 0 |
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