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| 1 | Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Brassica rapa comprises several important cultivated vegetables and oil crops.Current reference genome assemblies of Brassica rapa are quite fragmented and not highly contiguous,thereby limiting extensive genetic and genomic analyses.Here,we report an improved assembly of the B.rapa genome(v3.0)using single-molecule sequencing,optical mapping,and chromosome conformation capture technologies(Hi-C).Relative to the previous reference genomes,our assembly features a contig N50 size of 1.45 Mb,representing a~30-fold improvement.We also identified a new event that occurred in the B.rapa genome~1.2 million years ago,when a long terminal repeat retrotransposon(LTR-RT)expanded.Further analysis refined the relationship of genome blocks and accurately located the centromeres in the B.rapa genome.The B.rapa genome v3.0 will serve as an important community resource for future genetic and genomic studies in B.rapa.This resource will facilitate breeding efforts in B.rapa,as well as comparative genomic analysis with other Brassica species. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 10 |
| 2 | Brassica rapa Genome 2.0: A Reference Upgrade through Sequence Re-assembly and Gene Re-annotation显示文摘 | Chengcheng Cai Xiaobo Wang Bo Liu Jian Wu Jianli Liang Yinan Cui Feng Cheng Xiaowu Wang | 2017 | Molecular Plant2017，10，4： | 6 |
| 3 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 4 | Activated cytotoxic lymphocytes promote tumor progression by increasing the ability of 3LL tumor cells to mediate MDSC chemoattraction via Fas signaling显示文摘Fas/FasL 系统播送细胞内部的 apoptotic 发信号,导致房间 apoptosis。然而,船边交货发信号也除了导致肿瘤房间 apoptosis 施加 non-apoptotic 功能。例如,船边交货发信号导致肺癌症肿瘤房间生产前列腺素 E2 (PGE2 ) 和成员导出 myeloid 的 suppressor 房间(MDSC ) 。激活的细胞毒素的 T 淋巴细胞(CTL ) 导致并且表示 FasL 在房间仍然是的 apoptosis 抵抗的肿瘤上在 CTL 开始的船边交货激活的 FasL,而是效果的高水平大部分不清楚。我们从 OT-1 老鼠净化了激活的 CD8 + T 房间,在肿瘤房间逃跑上评估了船边交货激活的规章的效果并且调查了相关机制。我们发现 CTL 导致了肿瘤细胞分泌 PGE2 和增加肿瘤经由船边交货发信号的 MDSC 的调停房间的 chemoattraction,它对肿瘤生长有利。我们的结果显示 CTL 可以参予肿瘤免疫者避免过程。就我们的知识而言,这是 CTL 在肿瘤逃跑由起一个作用的新奇机制。我们的调查结果含有经由对肿瘤的否定有免疫力的回答的减小提高 antitumor 免疫者反应的策略通过船边交货发信号由 CTL 支持了。 | Fei Yang Yinxiang Wei Zhijian Cai Lei Yu Lingling Jiang Chengyan Zhang Huanmiao Yan Qingqing Wang Xuetao Cao Tingbo Liang Jianli Wang | 2015 | Cellular & Molecular Immunology2015，12，1： | 6 |
| 5 | A naturally occurring variation in the BrMAM-3 gene is associated with aliphatic glucosinolate accumulation in Brassica rapa leaves显示文摘Glucosinolate profiles significantly vary among Brassica rapa genotypes.However,the molecular basis of these variations is largely unknown.In this study,we investigated a major quantitative trait locus(QTL)controlling aliphatic glucosinolate accumulation in B.rapa leaves.The QTL,which encompasses three tandem MAM genes and two MYB genes,was detected in two BC2DH populations.Among the five-candidate genes,only the expression level of BrMAM-3(Bra013007)was significantly correlated with the accumulation of aliphatic glucosinolates in B.rapa leaves.We identified a naturally occurring insertion within exon 1 of BrMAM-3,which is predicted to be a loss-of-function mutation,as confirmed by qRT-PCR.We determined that the loss of function was associated with the low glucosinolate content in B.rapa accessions.Furthermore,overexpressing the BrMAM-3 gene resulted in an increase in total aliphatic glucosinolates in Arabidopsis transgenic lines.Our study provides insights into the molecular mechanism underlying the accumulation of aliphatic glucosinolates in B.rapa leaves,thereby facilitating in the manipulation of total aliphatic glucosinolate content in Brassica crops. | Jifang Zhang Hui Wang Zhiyuan Liu Jianli Liang Jian Wu Feng Cheng Shiyong Mei Xiaowu Wang | 2018 | Horticulture Research2018，5，1： | 5 |
| 6 | Variation in Plant Morphology and Sinigrin Content in Ethiopian Mustard(Brassica carinata L.)显示文摘Ethiopian mustard(Brassica carinata) is mainly grown as a leafy vegetable and oilseed crop. Sinigrin is predominant glucosinolate in the leaves of Ethiopian mustard. It is hydrolyzed by enzyme myrosinase to produce bioactive product specifically allyl-isothiocyanates. Due to sinigrin content, Ethiopian mustard has a mild flavor and it is a healthy addition to human diet. However, sinigrin concentration depends on genotype.Understanding variations of plant morphology and sinigrin content may be the first step for crop improvement. According to the analyses of various plant characteristics among ninety nine Ethiopian mustard accessions, we found significant differences in plant height, chlorophyll content, and petiole length. In addition, sinigrin content was determined using ultra-performance liquid chromatography tandem quadrupole mass spectrometers/tunable ultraviolet detector(UPLC-TQS/TUV). We found a significant difference in sinigrin content among leaves of 94 Ethiopian mustard accessions. Except for petiole length, the sinigrin content was negatively correlated with leaf area, leaf width, number of primary branches, and plant height. This information will help Ethiopian mustard breeders in screening breeding lines for new accessions. | Tesfay Teklehaymanot Haijing Wang Jianli Liang Jian Wu Runmao Lin Zhen Zhou Xu Cai Xiaowu Wang | 2019 | Horticultural Plant Journal2019，5，5： | 4 |
| 7 | Hotspots of Independent and Multiple Rounds of LTR-retrotransposon Bursts in Brassica Species显示文摘Long terminal repeat retrotransposons(LTR-RTs) are a predominant group of plant transposable elements(TEs) that are an important component of plant genomes. A large number of LTR-RTs have been annotated in the genomes of the agronomically important oil and vegetable crops of the genus Brassica. Herein, full-length LTR-RTs in the genomes of Brassica and other closely related species were systematically analyzed. The full-length LTR-RT content varied greatly(from 0.43% to 23.4%) between different species, with Gypsy-like LTR-RTs constituting a primary group across these genomes. More importantly, many annotated LTR-RTs(from 10.03% to 33.25% of all detected LTR-RTs) were found to be enriched in localized hotspot regions. Furthermore, all of the analyzed species showed evidence of having experienced at least one round of a LTR-RT burst, with Raphanus sativus experiencing three or more. Moreover, these relatively ancient LTR-RT amplifications exhibited a clear expansion at specific time points. To gain a further understanding of this timing, Brassica rapa, B. oleracea, and R. sativus were examined for the presence of syntenic regions, but none were present. These findings indicate that these LTR-RT burst events were not inherited from a common ancestor,but instead were species-specific bursts that occurred after the divergence of Brassica species. This study further exemplifies the complexities of TE amplifications during the evolution of plant genomes and suggests that these LTR-RT bursts play an important role in genome expansion and divergence in Brassica species. | CAI Xu CUI Yinan ZHANG Lei WU Jian LIANG Jianli CHENG Lin WANG Xiaowu CHENG Feng | 2018 | Horticultural Plant Journal2018，4，4： | 3 |
| 8 | A High Density Linkage Map Facilitates QTL Mapping of Flowering Time in Brassica rapa显示文摘Flowering is a major developmental transition in the life-history of plants. Flowering time is under complex genetic control. In this study, 172 doubled haploid(DH) lines derived from a cross between two Chinese cabbage(Brassica rapa ssp. pekinensis) DH lines, Y177 and Y195, were used for a high density linkage map construction and quantitative trait locus(QTL) mapping for flowering time in B. rapa. Parents and DH lines were resequenced at depth of 5× and 0.5× coverage, respectively. In total, 22 747 SNPs were called from the resequencing data and combined into 1 708 bins to construct the genetic linkage map. The map length was 958.6 c M, containing 887 bins and the average interval between binmarkers was 1.08 c M. A total of six QTLs controlling flowering time were detected under four different conditions. Potential candidate flowering homologues located near QTLs were identified with the aid of B. rapa genome annotation. | LIU Jing LIU Bo CHENG Feng LIANG Jianli WANG Xiaowu WU Jian | 2016 | Horticultural Plant Journal2016，2，4： | 3 |
| 9 | Segmental Translocation Contributed to the Origin of the Brassica S-locus显示文摘Self-incompatibility(SI),which has recurred during the evolution of plants,is one of the most important cross-pollination mating systems.Three S-loci have been reported in Brassicaceae,namely,Arabidopsis lyrata(Al),Brassica(Br),and Leavenworthia alabamica(La)S-loci.Here,through multi-genomic comparative analysis of 20 species,we revealed that the most ancient S-locus was formed prior to the divergence of Brassicaceae lineage I and II.Itwas retained and inherited by Arabidopsis,as the Al S-locus in Brassicaceae lineage I.Furthermore,we found that the Br S-locus,which has been widely used in the breeding of Brassica crops to generate hybrid seeds,was formed through segmental translocation(ST)in the hexaploid ancestor of Brassica in Brassicaceae lineage II.The Br S-locus was evolved through a ST from one of the triplicated ancestral S-locus paralogs in the Brassica hexaploidy ancestor,while the other two S-locus paralogs were lost.Together with the previous discovery that the La S-locus was formed through a secondary origin in Brassicaceae lineage I,we conclude the monophyletic origin of Al and Br S-loci and clarify the evolutionary route of S-loci in the Brassicaceae family.Our findings will contribute to evolutionary studies and breeding applications of the S-locus in Brassicaceae. | Yinan Cui Mu Zhuang Jian Wu Jisheng Liu Yiyue Zhang Lingkui Zhang Yile Huang Xu Cai Jianli Liang Kang Zhang Xiaowu Wang Feng Cheng | 2020 | Horticultural Plant Journal2020，6，3： | 2 |
| 10 | Identification and characterization of phosphodiesterases that specifically degrade 3'3'-cyclic GMP-AMP显示文摘 | Juyi Gao Jianli Tao Weili Liang Meng Zhao Xiaoxia Du Shan Cui Haifeng Duan Biao Kan Xiaodong Su Zhengfan Jiang | 2015 | Cell Research2015，25，5： | 2 |
| 11 | Author Correction:Improved Brassica rapa reference genome by single-molecule sequencing and chromosome conformation capture technologies显示文摘Since the publication of this article,the authors have noticed that the total gene models(45,985),tandem arrays(2077),tandem genes(4963),redundancy removed(43,099),syntenic genes(39,858),nonsyntenic genes(3241),genes on chromosomes(45,411),genes on scaffolds(574)of B.rapa reference genome v3.0 were mistaken in the article. | Lei Zhang Xu Cai Jian Wu Min Liu Stefan Grob Feng Cheng Jianli Liang Chengcheng Cai Zhiyuan Liu Bo Liu Fan Wang Song Li Fuyan Liu Xuming Li Lin Cheng Wencai Yang Mai-he Li Ueli Grossniklaus Hongkun Zheng Xiaowu Wang | 2019 | Horticulture Research2019，6，1： | 2 |
| 12 | Experimental Investigation and Thermodynamic Reassessment of the Cu-Fe-Si System显示文摘 | Jingrui Zhao Lijun Zhang Yong Du Honghui Xu Jianlie Liang Baiyun Huang | 2009 | Metallurgical and Materials Transactions A2009，，8： | 1 |
| 13 | Breeding biology of the Green-backed Tit(Parus monticolus)in southwest China显示文摘Studies on breeding biology enable us to broaden our understanding of the evolution of life history strategies.We studied the breeding biology of the Green-backed Tit(Parus monticolus)to provide comprehensive data on nest and egg characteristics,parental behavior throughout egg laying and nestling periods,and reproductive outcome.Our study reveals adaptive behavioral patterns and reproductive strategies for P.monticolus. | Ping Ye Xiaogang Yao Jianli Bi Guangrong Li Wei Liang Canchao Yang | 2021 | Avian Research2021，12，4： | 1 |
| 14 | In-situ stress state in the China’s Sea and adjacent areas显示文摘 | Jianli Gao Jianmin Ding Guoping Liang Dahuang Xia Qiliang Guo | 1992 | Acta Seismologica Sinica1992，，3： | 1 |
| 15 | In-situ stress state in the China’s Sea and adjacent areas显示文摘 | Jianli Gao Jianmin Ding Guoping Liang Dahuang Xia Qiliang Guo | 1992 | Acta Seismologica Sinica1992，，3： | 1 |
| 16 | Application of Drug Nanocrystal Technologies on Oral Drug Delivery of Poorly Soluble Drugs显示文摘 | Lei Gao Guiyang Liu Jianli Ma Xiaoqing Wang Liang Zhou Xiang Li Fang Wang | 2013 | Pharmaceutical Research2013，，2： | 1 |
| 17 | Intermetallics and phase transformations of the Zr-1.0Sn-0.3Nb-0.3Fe-0.1Cr alloy显示文摘Intermetallics and phase transformations of the zirconium-based alloy, Zr-1.0Sn-0.3Nb-0.3Fe-0.1Cr, were investigated by conventional X-ray diffraction (XRD), differential scanning calorimetry (DSC), and dilation measurement. Three types of precipitates, namely, (ZrNb)2Fe, Zr(CrFe)2, and Zr3Fe, were detected by XRD. The cubic Ti2Ni-type (ZrNb)2Fe was found to be the main precipitate in the alloy, and it was proposed to dissolve at 861°C, whereas Zr3Fe dissolved at 780°C and Zr(CrFe)2 at 814°C. No precipitates were observed at a temperature higher than 900°C. The transformation-start temperature of α-Zr →β-Zr was reconfirmed to be 780°C, and the end temperature of α-Zr →β-Zr was determined to be 955°C. The dilation result also revealed that the martensitic transformation-start temperature, Ms, and the finish temperature, Mf, of this alloy were 741°C and 645°C, respectively. | LIANG Jianlie TANG Yiyuan NONG Liangqin YAN Jialin ZHUANG Yinghong | 2008 | Rare Metals2008，27，5： | 1 |
| 18 | Drug nanocrystals: In vivo performances显示文摘 | Lei Gao Guiyang Liu Jianli Ma Xiaoqing Wang Liang Zhou Xiang Li | 2012 | Journal of Controlled Release2012，，3： | 1 |
| 19 | Development of InDel Markers for Brassica rapa Based on a High-resolution Melting Curve显示文摘Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China.To increase the availability of DNA markers in B.rapa,we developed insertion-deletion(InDel)markers utilizing high-resolution melting(HRM)curve analysis.We designed primers for 252 InDels(≥3 bp)evenly distributed in the genome and tested gene polymorphisms with eight accessions.In total,208 markers were specifically amplified,and 148 InDels with polymorphism were genotyped successfully using HRM.We further analyzed the correlation with InDel size,GC number,and predicted the difference in Tm values(Tm)using 208 markers with specific amplification.We found that the success rate of InDel markers was correlated with the GC number of InDel and the predicted-Tm,but not clearly correlated with the length of InDel.When the GC number within InDel was≥8,the successful rate exceeded 90.0%.When the predicted-Tm reached 0.5°C,the success rate was greater than 90.0%,and when it was≥0.6°C,the rate climbed to 100.0%,indicating their role as the optimal parameter for successful development of an applicable InDel marker.The polymorphic InDel markers can be easily genotyped using HRM.They are of great value in genetic analysis,construction of linkage map,and molecular marker-assisted selection in B.rapa. | Rong Chen Lichun Chang Xu Cai JianWu Jianli Liang Runmao Lin Yong Song Xiaowu Wang | 2021 | Horticultural Plant Journal2021，7，1： | 1 |
| 20 | A green route for hydrogen production from alkaline thermal treatment(ATT)of biomass with carbon storage显示文摘Hydrogen,a green energy carrier,is one of the most promising energy sources.However,it is currently mainly produced from depleting fossil fuels with high carbon emissions,which has serious negative effects on the economy and environment.To address this issue,sustainable hydrogen production from bio-energy with carbon capture and storage(HyBECCS)is an ideal technology to reduce global carbon emissions while meeting energy demand.This review presents an overview of the latest progress in alkaline thermal treatment(ATT)of biomass for hydrogen production with carbon storage,especially focusing on the technical characteristics and related challenges from an industrial application perspective.Additionally,the roles of alkali and catalyst in the ATT process are critically discussed,and several aspects that have great influences on the ATT process,such as biomass types,reaction parameters,and reactors,are expounded.Finally,the potential solutions to the general challenges and obstacles to the future industrial-scale application of ATT of biomass for hydrogen production are proposed. | Guojie Liu Zexue Du Houfang Lu Jianli Zeng Kejing Wu Bin Liang | 2023 | Carbon Resources Conversion2023，6，4： | 0 |
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