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| 1 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 2 | Two new prenylflavonoids from Morus nigra L.显示文摘二新 prenylflavonoids, mornigrol E (1 ) 和 mornigrol F (2 ) 从桑属 nigra L 被孤立。他们的结构根据分光镜的方法被阐明,特别 2D-NMR 技术。 | Lei Wang Ting Gong Ruo Yun Chen | 2009 | Chinese Chemical Letters2009，20，12： | 8 |
| 3 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 4 | A New Isoprenylated flavonol from the Leaves of Broussonetia kazinoki显示文摘A new isoprenylated flavonol, named broussonol A (1) was isolated from the leaves of Broussonetia kazinoki Sieb. The structure of broussonol A was characterized by chemical and spectral methods. | Pei Cheng ZHANG, Si WANG, Ying Hong WANG, Ruo Yun CHEN, De Quan YU* Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 | 2001 | Chinese Chemical Letters2001，12，2： | 5 |
| 5 | STRUCTURE ELUCIDATION OF LIGUSTILONE FROM LIGUSTICUM SINENSIS OLIV显示文摘STRUCTUREELUCIDATIONOFLIGUSTILONEFROMLIGUSTICUMSINENSISOLIV￥DeQuanYU;RuoYunCHEN;andFengZhiXIE.(InstituteofMateriaMedica,Chine... | De Quan YU Ruo Yun CHEN and Feng Zhi XIE.(Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,100050) | 1995 | Chinese Chemical Letters1995，6，5： | 4 |
| 6 | A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid) film for detection of pazufloxacin mesilate显示文摘A novel capacitive sensor for pazufloxacin mesilate (pazufloxacin) determination was developed by electropolymerizing p-aminobenzene sulfonic (p-ABSA) and molecularly imprinted polymers (MIPs), which was synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethyl-ene glycol dimethacrylate (EGDMA) in the presence of pazufloxacin template molecules, on the gold electrode surface. Furthermore, 1-dedecanethiol was used to insulate the modified electrode. Alter-nating current (ac) impedance experiments were carried out with a Model IM6e to obtain the capaci-tance responses. Under the optimum conditions, the sensor showed linear capacitance response to pazufloxacin in the range of 5 ng·mL-1 to 5 μg·mL-1 with a relative standard deviation (RSD) 5.3% (n=7) and a detection limit of 1.8 ng·mL-1. The recoveries for different concentration levels of pazufloxacin samples varied from 94.0% to 102.0%. Electrochemical experiments indicated the capacitive sensor exhibited good sensitivity and selectivity and showed excellent parameters of regeneration and stabil-ity. | ZHOU Lu YE GuangRong YUAN Ruo CHAI YaQin CHEN SuMing | 2007 | Science China Chemistry2007，50，4： | 4 |
| 7 | A New Diels-Alder Type Adduct and a New Flavone from the Stem and Root Bark of Morus mongolica显示文摘A new Diels-Alder type adduct mongolicin G (1) and a new flavone 5′-(1′′, 1′′-dimethyl- allyl)-5,7,2′,4′-tetrahydroxyflavone (2) were isolated from the stem and root bark of Morus mongolica. Their structures were determined by spectroscopic analysis and chiroptical methods. | Jie KANG Ruo Yun CHEN De Quan YU | 2005 | Chinese Chemical Letters2005，16，11： | 3 |
| 8 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 9 | STUDIES ON THE STRURE OF LIGUSTIPHENOL FROM LIGUSTICUM SINENCE OLIV显示文摘STUDIES ON THE STRURE OF LIGUSTIPHENOL FROM LIGUSTICUM SINENCE OLIV.￣1)STUDIESONTHESTRUREOFLIGUSTIPHENOLFROMLIGUSTICUMSINENCE... | De Quan YU Feng Zhi XIE Ruo Yun CHEN and Yan He HUANG(Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050) | 1996 | Chinese Chemical Letters1996，7，8： | 2 |
| 10 | Benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids:Design,synthesis and in vitro anti-mycobacterial activity evaluation显示文摘A series of novel benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids were designed, synthesized and evaluated for their in vitro anti-TB activities against drug-sensitive MTB H_(37)Rv and MDR-TB isolates as well as cytotoxicity. All benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids exhibited considerable in vitro anti-mycobacterial activities against the tested three MTB strains, and all of them also showed acceptable cytotoxicity. The most active hybrid 7f was >4.8 and >51 folds more potent than the first line anti-TB agents RIF and INH against both drug-sensitive MTB H_(37)Rv and MDR-TB isolates, respectively. The results demonstrated the potential utility of benzofuran-isatin-hydroxylimine/-thiosemicarbazide hybrids as anti-TB agents. | Yaohuan Zhang Ruo Wang Tesen Zhang Weitao Yan Yihong Chen Yanping Zhang Muyang Zhou | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 11 | Alkaloids from the Roots of Saccopetalum prolificum显示文摘A new, alkaloid. named prolilfine (1), was isolated along with four known alkaloids. liriodenine (2). 6-hydroxyonychine (3). isooncodine (4) and discretamine (5) from the roots of Saccopetalum prolificum. The structure of I was elucidated on the basis of spectroscopic and chemical methods. | Ming Lei WANG Jiang DU Ruo Yun CHEN De Quan YU (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050) | 2000 | Chinese Chemical Letters2000，11，2： | 2 |
| 12 | Mechanism of Learning and Memory Impairment in Rats Exposed to Arsenic and/or Fluoride Based on Microbiome and Metabolome显示文摘Objective Arsenic(As) and fluoride(F) are two of the most common elements contaminating groundwater resources. A growing number of studies have found that As and F can cause neurotoxicity in infants and children, leading to cognitive, learning, and memory impairments. However, early biomarkers of learning and memory impairment induced by As and/or F remain unclear. In the present study, the mechanisms by which As and/or F cause learning memory impairment are explored at the multi-omics level(microbiome and metabolome).Methods We stablished an SD rats model exposed to arsenic and/or fluoride from intrauterine to adult period.Results Arsenic and/fluoride exposed groups showed reduced neurobehavioral performance and lesions in the hippocampal CA1 region. 16S rRNA gene sequencing revealed that As and/or F exposure significantly altered the composition and diversity of the gut microbiome, featuring the Lachnospiraceae_NK4A136_group, Ruminococcus_1, Prevotellaceae_NK3B31_group, [Eubacterium]_xylanophilum_group. Metabolome analysis showed that As and/or F-induced learning and memory impairment may be related to tryptophan, lipoic acid, glutamate, gamma-aminobutyric acidergic(GABAergic) synapse, and arachidonic acid(AA) metabolism. The gut microbiota, metabolites, and learning memory indicators were significantly correlated.Conclusion Learning memory impairment triggered by As and/or F exposure may be mediated by different gut microbes and their associated metabolites. | ZHANG Xiao Li YU Sheng Nan QU Ruo Di ZHAO Qiu Yi PAN Wei Zhe CHEN Xu Shen ZHANG Qian LIU Yan LI Jia GAO Yi LYU Yi YAN Xiao Yan LI Ben REN Xue Feng QIU Yu Lan | 2023 | Biomedical and Environmental Sciences2023，36，3： | 2 |
| 13 | Two new diterpenoids from Suregada glomerulata(Blume) Baill显示文摘二新 diterpenoids, 3,4,18-cyclopropa-ent-abieta-8 (14 ) , 13 (15 )-dien-16,12-olide (1 ) 和 3-oxojolkinolide B (2 ) ,从 Suregada glomerulata (布鲁坶) 的根被孤立 Baill。他们的结构被分光镜的证据决定。加重 2 显示出的中等 cytotoxicity。 | Ren Yi Yan Ruo Yun Chen De Quan Yu | 2011 | Chinese Chemical Letters2011，22，5： | 1 |
| 14 | Amperometric Hydrogen Peroxide Biosensor Based on the Immobi- lization of Horseradish Peroxidase (HRP) on the Layer by-Layer Assembly Films of Gold Colloidal Nanoparticles and Toluidine Blue 显示文摘 | CHEN Shi-hong YUAN Ruo CHAI Ya-qin | 2006 | Electroanalysis2006，，18： | 1 |
| 15 | Recent Progress in Polymer Solar Cells: Manipulation of Polymer: Fullerene Morphology and the Formation of Ef{icient Inverted Polymer Solar Cells 显示文摘 | CHEN Li-min HONG Zu ruo LI Gang | 2009 | Adv Mater2009，21，： | 1 |
| 16 | Exchange of water masses between the East China Sea and the Kuroshio off northeastern Taiwan 显示文摘 | CHEN C T A RUO R PAI S C | 1995 | Continental Shelf Research1995，15，1： | 1 |
| 17 | Amperometric third-generation hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles显示文摘 | Shihong Chen Ruo Yuan Yaqin Chai Lingyan Zhang Na Wang Xuelian Li | 2006 | Biosensors and Bioelectronics2006，，7： | 1 |
| 18 | Three-dimensional response of unrelaxed tension to instability ofviscoelastic jets 显示文摘 | Ruo A N C Chen F Chung GANG | 2011 | Journal of Fluid Mechanics2011，682，1： | 1 |
| 19 | Exchange of water masses between the East-China-Sea and the Kuroshio off northeastern Taiwan显示文摘 | Chen C T A Ruo R Pai S C | 1995 | Continental Shelf Research1995，15，1： | 1 |
| 20 | Monitoring System of City Water Supply Pipe Network Based on Zigbee and GPRS显示文摘 | Hong Tao Wang Ruo Yu Wang Xiao Chen Xiao Bo Bai Zeng Xiang Dong Gang Chang Ji | 2014 | Applied Mechanics and Materials2014，，441： | 1 |
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