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| 1 | Hantavirus infection:a global zoonotic challenge显示文摘Hantaviruses are comprised of tri-segmented negative sense single-stranded RNA, and are members of the Bunyaviridae family. Hantaviruses are distributed worldwide and are important zoonotic pathogens that can have severe adverse effects in humans. They are naturally maintained in specific reservoir hosts without inducing symptomatic infection. In humans, however,hantaviruses often cause two acute febrile diseases, hemorrhagic fever with renal syndrome(HFRS) and hantavirus cardiopulmonary syndrome(HCPS). In this paper, we review the epidemiology and epizootiology of hantavirus infections worldwide. | Hong Jiang Xuyang Zheng Limei Wang Hong Du Pingzhong Wang Xuefan Bai | 2017 | Virologica Sinica2017，32，1： | 47 |
| 2 | Regional assessment of cadmium pollution in agricultural lands and the potential health risk related to intensive mining activities: A case study in Chenzhou City, China显示文摘The purpose of this study was to assess the extent of cadmium (Cd) contamination in agricultural soil and its potential risk for people. Soils, rice, and vegetables from Chenzhou City, Southern China were sampled and analyzed. In the surface soils, the 95% confidence interval for the mean concentration of Cd varied between 2.72 and 4.83 mg/kg (P < 0.05) in the survey, with a geometric mean concentration of 1.45 mg/kg. Based on the GIS map, two hot spot areas of Cd in agricultural soils with high Cd concentrations were identified to be located around the Shizhuyuan, Jinshiling, and Yaogangxian mines, and the Baoshan and Huangshaping mines, in the center of the city. About 60% of the total investigated area, where the agricultural soil Cd concentration is above 1 mg/kg, is distributed in a central belt across the region. The critical distances, at which the soil Cd concentration were increased by the mining activities, from the mines of the soils were 23 km for the Baoshan mine, 46 km for the Huangshaping mine, and 63 km for the Shizhuyuan mine, respectively. These are distances calculated from models. The Cd concentrations in rice samples ranged from 0.01 to 4.43 mg/kg and the mean dietary Cd intake from rice for an adult was 191 μg/d. Results of risk indexes showed that soil Cd concentrations possessed risks to local residents whose intake of Cd from rice and vegetables grown in soils in the vicinity of the mine was 596 μg/d. | ZHAI Limei LIAO Xiaoyong CHEN Tongbin YAN Xiulan XIE Hua WU Bin WANG Lixia | 2008 | Journal of Environmental Sciences2008，20，6： | 35 |
| 3 | Heavy metal concentrations of agricultural soils and vegetables from Dongguan, Guangdong显示文摘118 农业土壤和 43 件蔬菜样品的一个总数从 Dongguan 城市被收集,广东,中国。空间分发,来源,累积特征和在农业土壤和蔬菜的重金属的潜在的风险被三条不同途径在细节描绘,包括在土壤和蔬菜的八个金属元素的全部的内容,在在学习的土壤的重金属的 GIS 地图和 multivariate 分析。结果证明在农业土壤有象 Cu , Zn , Ni , Pb , Cd 和 Hg 那样的重金属的更高的累积,并且 Pb 的内容( 65.38 mg kg ? 2 )并且 Hg ( 0.24 mg kg ? 2 )分别地是在广东省的土壤的相应重金属的背景内容的 1.82 和 2.82 次。有在附近的 3.4% Cu, 5.9% Ni, 1.7% Cd 和 28% Hg 在所有从有的所有调查地点收集了土壤样品溢出为为土壤标准的中国环境质量的重金属的内容(GB15618-1995,等级 II ) 。在土壤的多金属的污染特征被 Hg 主要反映。到在土壤的八个金属元素有不同来源, Cu, Zn, Ni, Cr 并且这是主要源于父母材料,并且 Pb, Hg 和 Cd 被人为的活动影响。空间分发表演 Cu, Zn, Ni, Cr, Pb 并且农业土壤的 Hg 内容在西方高并且在东方,和 Cd 内容低在西北高,东南并且在在 Dongguan 的西南低。Ni 的蔬菜样品的比率, Pb 并且作为比最大值高的集中,在食物(GB2762-2005 ) 的沾染物的层次分别地是 4.7% , 16.3% 和 48.8% 。在蔬菜的重金属的简历集中因素(BCF ) 的顺序是 Cd > Zn > Cu > 作为 > Ni > Hg > Cr > Pb。在 Dongguan 城市里从农业土壤和蔬菜为食物安全和人健康集中于重金属的潜在的风险是必要的,广东省。 | CAI Limei HUANG Lanchun ZHOU Yongzhang XU Zhencheng PENG Xiaochun YAO Ling'ai ZHOU Yang PENG Ping'an | 2010 | Journal of Geographical Sciences2010，20，1： | 23 |
| 4 | Soil pH determines fungal diversity along an elevation gradient in Southwestern China显示文摘Fungi play important roles in ecosystem processes, and the elevational pattern of fungal diversity is still unclear. Here, we examined the diversity of fungi along a 1,000 m elevation gradient on Mount Nadu, Southwestern China. We used MiSeq sequencing to obtain fungal sequences that were clustered into operational taxonomic units(OTUs) and to measure the fungal composition and diversity. Though the species richness and phylogenetic diversity of the fungal community did not exhibit significant trends with increasing altitude, they were significantly lower at mid-altitudinal sites than at the base. The Bray-Curtis distance clustering also showed that the fungal communities varied significantly with altitude. A distance-based linear model multivariate analysis(DistLM) identified that soil pH dominated the explanatory power of the species richness(23.72%),phylogenetic diversity(24.25%) and beta diversity(28.10%) of the fungal community. Moreover, the species richness and phylogenetic diversity of the fungal community increased linearly with increasing soil pH(P<0.05). Our study provides evidence that pH is an important predictor of soil fungal diversity along elevation gradients in Southwestern China. | Dan Liu Guohua Liu Li Chen Juntao Wang Limei Zhang | 2018 | Science China(Life Sciences)2018，61，6： | 21 |
| 5 | Tumor-infiltrating B cells:their role and application in antitumor immunity in lung cancer显示文摘Evidence indicates that lung cancer development is a complex process that involves interactions between tumor cells,stromal fibroblasts,and immune cells.Tumor-infiltrating immune cells play a significant role in the promotion or inhibition of tumor growth.As an integral component of the tumor microenvironment,tumor-infiltrating B lymphocytes(TIBs)exist in all stages of cancer and play important roles in shaping tumor development.Here,we review recent clinical and preclinical studies that outline the role of TIBs in lung cancer development,assess their prognostic significance,and explore the potential benefit of B cell-based immunotherapy for lung cancer treatment. | Si-si Wang Wei Liu Dalam Ly Hao Xu Limei Qu Li Zhang | 2019 | Cellular & Molecular Immunology2019，16，1： | 21 |
| 6 | Subcellular distribution and compartmentalization of arsenic in Pteris vittata L.显示文摘The subcellular distribution of arsenic (As) in Pteris vittata L., an As-hyperaccumulator, was studied to de- termine As compartmentalization and to explore the mecha- nisms that confer As tolerance. When the plant was grown in a nutrient solution without additional As, most of the accu- mulated As was isolated to the cell wall. However, in plants growing in a nutrient solution containing 0.1 or 0.2 mmol/L As, approximately 78% of the total As accumulated within the pinna. The proportions of As accumulation in the cyto- plasmic supernatant fraction were 78% of that in the pinna and 61% of that in the plant. In either treatment group (0.1 or 0.2 mmol/L As), the fraction containing the lowest level of As was the organelle fraction. These results suggest that As accumulates in the pinna where it is primarily distributed in the cytoplasmic supernatant fraction. The role of As com- partmentalization may be intricately linked with As detoxi- fication in P. vittata L. | CHEN Tongbin YAN Xiulan LIAO Xiaoyong XIAO Xiyuan HUANG Zechun XIE Hua ZHAI Limei | 2005 | Chinese Science Bulletin2005，50，24： | 17 |
| 7 | Human avian influenza A (H5N1) virus infection in China显示文摘Highly pathogenic influenza A (H5N1) virus causes a widespread poultry deaths worldwide. The first human H5N1 infected case was reported in Hong Kong Special Administrative Region of China in 1997. Since then, the virus re-emerged in 2003 and continues to infect people worldwide. Currently, over 400 human infections have been reported in more than 15 countries and mortality rate is greater than 60%. H5N1 viruses still pose a potential pandemic threat in the future because of the continuing global spread and evolution. Here, we summarize the epidemiological, clinical and virological characteristics of human H5N1 infection in China monitored and identified by our national surveillance systems. | XU CuiLin, DONG LiBo, XIN Li, LAN Yu, CHEN YongKun, YANG LiMei & SHU YueLong State Key Laboratory for Viral Genetic and Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 100052, China | 2009 | Science China(Life Sciences)2009，52，5： | 14 |
| 8 | 3D Positional and Quantitative Prediction of the Xiaoqinling Gold Ore Belt in Tongguan, Shaanxi, China显示文摘Quantitative prediction of deep orebody based on 3D visualization technology is of great significance in mineral exploration. Based on the 2D traditional quantitative predicting method, the geoanomaly theory and the mineral exploration model idea, we constructed 3D models of the topography, strata, structure, magmatite and prospecting engineering of the study area using the commercial 3D modeling software Micromine, delineated eight prospective areas and estimated the gold resources amount with methods of Abundance Estimation and Volume Estimation. Then, we compared and counted the known ore blocks and the predicted blocks, which quantitatively explains this prediction's validity. The results show that Xiaoqinling gold belt in Tongguan has convincing potential for gold development and utilization and the prediction method based on 3D visualization technology proves to be effective. | CHEN Jianping SHI Rui CHEN Zhenping WANG Limei SUN Yan | 2012 | Acta Geologica Sinica(English Edition)2012，86，3： | 14 |
| 9 | Microstructure and wear resistance of PTA clad Cr_7C_3/γ-Fe ceramal composite coating显示文摘A 穿抵抗 Cr 7 C 3/γ-Fe 合金陶瓷合成涂层在变硬和 tempering C 度钢 by6 PTA 的底层上被制作(转移的血浆是) cladding 与(wt%) Fe-25Cr-7C 元素的粉末相配。涂层的微观结构被 OM, SEM, XRD 和版本描绘穿涂层的抵抗在干燥滑动下面被测试在房间温度穿状况。结果显示合成涂层有的 PTA 穿着的合金陶瓷由 Cr 7 C 3 主要粒子一致地在 γ-Fe 矩阵散布了,冶金学的联盟者被结合到 C 度钢底层。PTA 穿着的 Cr 7 C 3/Fe ceremal 合成涂层有高坚硬并且优秀在干燥滑动下面穿抵抗穿测试条件。优秀穿 Cr 7 C 3/γ-Fe ceremal 合成涂层被归因于涂层的高坚硬。 | LIU Junbo WANG Limei HUANG Jihua | 2006 | Progress in Natural Science:Materials International2006，16，8： | 13 |
| 10 | Middle Triassic post-orogenic extension on Hainan Island: Chronology and geochemistry constraints of bimodal intrusive rocks显示文摘This work studies new petrological and geochemical data and zircon U-Pb ages for bimodal intrusive rocks from Xinglong region, Hainan Island. Zircon U-Pb dating yields mean 238 U/ 206 Pb ages of 238 ± 2 Ma and 234 ± 2 Ma for diabasic and granitic phases, respectively, representing the Middle Triassic emplacement. The diabase and granite bodies were formed from discrete mafic and acidic magma sources that experienced local mechanical mixing at their mutual contacts. Although SiO 2 content of intrusions is bimodal, trace element ratios indicate that both were formed in a post-orogenic extensional setting. Sr-Nd isotopic composition reflects a shared EMII type enriched mantle source component, possibly influenced by subduction-fluid metasomatism. | TANG LiMei CHEN HanLin DONG ChuanWan YANG ShuFeng SHEN ZhongYue CHENG XiaoGan FU LuLu | 2013 | Science China Earth Sciences2013，56，5： | 12 |
| 11 | Dynamic Changes in the Wetland Landscape Pattern of the Yellow River Delta from 1976 to 2016 Based on Satellite Data显示文摘The Yellow River Delta wetland is the youngest wetland ecosystem in China's warm temperate zone. To better understand how its landscape pattern has changed over time and the underlying factors responsible, this study analyzed the dynamic changes of wetlands using five Landsat series of images, namely MSS(Mulri Spectral Scanner), TM(Thematic Mapper), and OLI(Operational Land Imager) sensors in 1976, 1986, 1996, 2006, and 2016. Object-oriented classification and the combination of spatial and spectral features and both the Normalized Difference Vegetation Index(NDVI) and Normalized Difference Water Index(NDWI), as well as brightness characteristic indices, were used to classify the images in eCognition software. Landscape pattern changes in the Yellow River Delta over the past 40 years were then delineated using transition matrix and landscape index methods. Results show that: 1) from1976 to 2016, the total area of wetlands in the study area decreased from 2594.76 to 2491.79 km^2, while that of natural wetlands decreased by 954.03 km^2 whereas human-made wetlands increased by 851.06 km^2. 2) The transformation of natural wetlands was extensive: 31.34% of those covered by Suaeda heteropteras were transformed into reservoirs and ponds, and 24.71% with Phragmites australis coverage were transformed into dry farmland. Some human-made wetlands were transformed into non-wetlands types: 1.55% of reservoirs and ponds became construction land, and likewise 21.27% were transformed into dry farmland. 3) From 1976 to 2016, as the intensity of human activities increased, the number of landscape types in the study area continuously increased. Patches were scattered and more fragmented. The whole landscape became more complex. In short, over the past 40 years, the wetlands of the Yellow River Delta have been degraded, with the area of natural wetlands substantially reduced. Human activities were the dominant forces driving these changes in the Yellow River Delta. | CONG Pifu CHEN Kexin QU Limei HAN Jianbo | 2019 | Chinese Geographical Science2019，29，3： | 12 |
| 12 | Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria显示文摘Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis (DGGE) followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria. | SHI Limei CAI Yuanfeng YANG Hualin XING Peng LI Pengfu KONG Lingdong KONG Fanxiang | 2009 | Journal of Environmental Sciences2009，21，11： | 11 |
| 13 | Heterotic Group Classification of 63 Inbred Lines and Hybrid Purity Identification by Using SSR Markers in Winter Cabbage(Brassica Oleracea L. var. capitata)显示文摘Winter cabbage is an important crop cultivated through winter in the region near the Yangtze River, enabling the supply of fresh cabbage there at that time of year. However, a problem has emerged regarding the newly developed parents of winter cabbage, which is completely different from spring and autumn cabbage, namely, how to combine these parents to breed an elite hybrid. To classify the heterotic groups and improve the efficiency of parent selection in winter cabbage breeding, 20 polymorphic SSR markers were selected to screen 63 winter cabbage inbred lines. Seventeen pairs among the 20 SSR markers amplified polymorphic bands. These primers amplified two to six bands,with an average of 2.8 bands per primer, and a total of 47 polymorphic bands were generated in the 63 inbred lines. These lines included flatheaded morphotype and round-headed morphotype, thus they were separately classified into heterotic groups based on the SSR markers. The flat-headed morphotype contained 21 inbred lines and was classified into three heterotic groups, named Hanchun 4, Jiali, and Dongsheng, in accordance with the representative germplasm contained in each group. The round-headed morphotype contained 42 inbred lines and was classified into five heterotic groups, named Parte, Bejo1039, YK-143, SCA002, and Golden B90. Meanwhile, parent analysis of 20 developed elite combinations showed that their parents were all distributed in different heterotic groups, indicating that the group classification was reasonable,which can provide a basis for further parent selection in winter cabbage breeding. Furthermore, polymorphic SSR primers were successfully used to identify the hybrid purity of three elite varieties. | LI Xing YU Hailong LI Zhiyuan LIU Xiaoping FANG Zhiyuan LIU Yumei YANG Limei ZHUANG Mu LV Honghao ZHANG Yangyong | 2018 | Horticultural Plant Journal2018，4，4： | 11 |
| 14 | Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli: Culturability, membrane permeability,metabolism, and genetic damage显示文摘Traditional culture methods may underestimate the tolerance of microorganisms to disinfectants because of the existence of viable but nonculturable or sublethally injured cells after disinfection.The selection of a strict method is crucial for the evaluation of disinfection performance.The actions of 2 typical disinfectants–ultraviolet(UV)and chlorine–on the fecal indicator Escherichia coli were investigated by the detection of culturability,membrane permeability,metabolic activity,deoxyribonucleic acid(DNA),and messenger ribonucleic acid(m RNA).During UV disinfection,the irreversible damages in the cell membrane and cellular adenosine triphosphate(ATP)were negligible at low UV doses(<80 m J/cm^2).However,membrane permeability was damaged at low doses of chlorine(<5 mg/L),leading to leakage of cellular ATP.Our study showed that a slight lesion in DNA was detected even at high doses of UV(400 m J/cm^2)and chlorine(>5 mg/L)treatments.The decay of m RNA was more rapid than that of DNA.The degradation level of m RNA depended on the choice of target genes.After exposure to 50 m J/cm^2UV dose or 5 mg/L chlorine for30 min,the DNA damage repair function(Rec A m RNA)was inhibited.The m RNA involved in the DNA damage repair function can be a potential indicator of bacterial viability. | Limei Xu Chongmiao Zhang Pengcheng Xu Xiaochang C.Wang | 2018 | Journal of Environmental Sciences2018，30，3： | 10 |
| 15 | Lyzl4, a novel mouse sperm-related protein, is involved in fertilization显示文摘鸡类型(c 类型) 溶解酵素的角色,原型溶解酵素,在免疫在许多有机体被描绘了。在这研究,我们克隆新奇 c 类型像溶解酵素的基因, Lyzl4,它位于老鼠染色体 9F4 并且与通常认为的信号肽和一个朊酶劈开地点编码了 145 氨基酸。在酵母表示的成熟 recombinant Lyzl4 蛋白质没显示出 bacteriolytic 活动。顺序排列分析证明在 c 类型溶解酵素的 20 不变的残余中的 3 个在 Lyzl4 被改变。改变的氨基酸(D52G ) 之一位于催化领域。Lyzl4 mRNA 有选择地在成年鼠标在睾丸和 epididymis 被表示,与改变越过不同发展阶段的表达式水平。Lyzl4 蛋白质的高水平在 acrosomal 区域和尾巴的主要的片的精子上被发现。在有它的特定的抗体的精子的 Lyzl4 蛋白质的免疫中立化显著地以一种剂量依赖者方式在 vitro 授精百分比减少了,建议 Lyzl4 可能为授精是重要的。 | Ruilin Sun Ruling Shen Jun Li Guojiang Xu Jun Chi Limei Li Jianke Ren Zhugang Wang Jian Fei | 2011 | Acta Biochimica et Biophysica Sinica2011，43，5： | 9 |
| 16 | PTA clad (Cr,Fe)_7C_3/γ-Fe in situ ceramal composite coating显示文摘A wear-resistant (Cr, Fe)7C3/γ-Fe in situ ceramal composite coating was fabricated on the substrate of 0.45wt%C carbon steel by a plasma-transferred arc cladding process using the Fe-Cr-C elemental powder blends. The microstructure, microhardness, and dry-sliding wear resistance of the coating were evaluated. The results indicate that the microstructure of the coating, which was composed of (Cr, Fe)7C3 primary phase uniformly distributed in the γ-Fe, and the (Cr, Fe)7C3 eutectic matrix was metallurgically bonded to the 0.45wt%C carbon steel substrate. From substrate to coating, the microstructure of the coating exhibited an evident epitaxial growth character. The coating, indehiscent and tack-free, had high hardness and appropriate gradient. It had excellent wear resistance under the dry sliding wear test condition. | Junbo Liu Limei Wang Jihua Huang | 2006 | Journal of University of Science and Technology Beijing2006，13，6： | 9 |
| 17 | Structural insight into substrate specificity of human intestinal maltase-glucoamylase显示文摘Human maltase-glucoamylase(MGAM)hydrolyzes linear alpha-1,4-linked oligosaccharide substrates,playing a crucial role in the production of glucose in the human lumen and acting as an efficient drug target for type 2 diabetes and obesity.The amino-and carboxyl-terminal portions of MGAM(MGAM-N and MGAM-C)carry out the same catalytic reaction but have different substrate specificities.In this study,we report crystal structures of MGAM-C alone at a resolution of 3.1Å,and in complex with its inhibitor acarbose at a resolution of 2.9Å.Structural studies,combined with biochemical analysis,revealed that a segment of 21 amino acids in the active site of MGAM-C forms additional sugar subsites(+2 and+3 subsites),accounting for the preference for longer substrates of MAGM-C compared with that of MGAM-N.Moreover,we discovered that a single mutation of Trp1251 to tyrosine in MGAM-C imparts a novel catalytic ability to digest branched alpha-1,6-linked oligosaccharides.These results provide important information for understanding the substrate specificity of alphaglucosidases during the process of terminal starch digestion,and for designing more efficient drugs to control type 2 diabetes or obesity. | Limei Ren Xiaohong Qin Xiaofang Cao LeleWang Fang Bai Gang Bai Yuequan Shen | 2011 | Protein & Cell2011，2，10： | 9 |
| 18 | Sterilization of Escherichia coli cells by the application of pulsed magnetic field显示文摘The inactivation of microorganisms by pulsed magnetic field was studied. It was improved that the application of electromagnetic pulses evidently causes a lethal effect on E. coli cells suspended in phosphate buffer solution Na 2HPO 4/NaH 2PO 4(0 334/0 867 mmol/L). Experimental results indicated that the survivability(N/N 0; where N 0 and N are the number of cells survived per mill il iter before and after electromagnetic pulses application, respectively) of E. coli decreased with magnetic field intensity B and treatment time t. It was also found that the medium temperatures, the frequencies of pulse f, and the initial bacterial cell concentrations have determinate influences in destruction of E. coli cells by the application of magnetic pulses. The application of an magnetic intensity B=160 mT at pulses frequency f=62 kHz and treatment time t=16 h result in a considerable destruction levels of E. coli cells (N/N 0=10 -4 ). Possible mechanisms involved in sterilization of the magnetic field treatment were discussed. In order to shorten the treatment time, many groups of parallel inductive coil were used. The practicability test showed that the treatment time was shortened to 4 h with the application of three groups of parallel coil when the survivability of E.coli cells was less than 0 01%; and the power consumption was about 0 2 kWh /m 3. | LIMei QUJiu-hui PENGYong-zhen | 2004 | Journal of Environmental Sciences2004，16，2： | 8 |
| 19 | Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress显示文摘The responses of activities, abundances and community structures of soil denitrifiers to mercury (Hg) stress were investigated through a short-term incubation experiment. Four soil treatments with different concentrations of Hg (CK, Hg25, Hg50, and Hg100, denoted as 0, 25, 50, and 100 mg Hg/kg dry soil, respectively) were incubated for 28 days. Soil denitrification enzyme activity (DEA) was measured at day 3, 7 and 28. The abundances and community structures of two denitrification concerning genes, nirS (cd1-nitrite reductase gene) and nosZ (nitrous oxide reductase gene), were analyzed using real-time PCR and denaturing gradient gel electrophoresis (DGGE). Results showed that soil DEA was significantly stimulated in the treatments of Hg25 and Hg50 compared with others at day 7. Meanwhile, no difference in the abundances of soil nirS and nosZ was found between Hg spiked treatments and CK, except the lower abundance of nirS (P < 0.05) in the Hg added treatments compared with that in the CK at day 28. The community structures of denitrifiers based on nirS gene presented obvious change at day 7 along with the Hg additions, however, no variation was found in all treatments based on the nosZ gene. The results indicated that Hg (Hg25 and Hg50) had a strongly short-term stimulation on soil DEA, and nirS gene is more sensitive than nosZ gene to Hg stress. | Zhifeng Zhou Yuanming Zheng Jupei Shen Limei Zhang Yurong Liu Jizheng He | 2012 | Journal of Environmental Sciences2012，24，3： | 8 |
| 20 | Parasympathetic neurogenesis is strongly associated with tumor budding and correlates with an adverse prognosis in pancreatic ductal adenocarcinoma显示文摘Objective: To investigate the frequency of parasympathetic neurogenesis and determine its association with tumor budding and prognosis in pancreatic ductal adenocarcinoma(PDAC).Methods: Parasympathetic neurogenesis was defined as the distribution of abnormal parasympathetic nerves in the stroma tissue. Staining of vesicular acetylcholine transporter(VACh T), as a marker for parasympathetic neurogenesis, was performed on a representative specimen of the tumor for 59 PDAC patients with available clinical, pathologic, and follow-up information. Three specimens containing normal pancreatic tissues were stained in parallel. The number of parasympathetic nerve fibers was counted in five high-power microscopic fields(5×0.785 mm^2). Cut-off values were calculated by receiver operating characteristic curve analysis.Results: VACh T-positive parasympathetic nerve fibers were not seen in the stroma of 3 cases of normal pancreatic tissues. In 59 PDAC cases, the range of parasympathetic neurogenesis was 4-38 fibers/(5×0.785) mm^2, with a median of 18 fibers/(5×0.785) mm^2. Patients with parasympathetic neurogenesis >15 fibers/(5×0.785) mm^2 were defined as the high-density group(39 patients, 66.1%), and those with parasympathetic neurogenesis ≤15 fibers/(5×0.785) mm^2 as the low-density group(20 patients, 33.9%). The high-density group had a higher occurrence of tumor budding(P=0.001) and a higher rate of early recurrence(P=0.035). Parasympathetic neurogenesis appeared to be an independent adverse prognostic factor [hazard ratio(HR)=2.45, 95% confidence interval(95% CI): 1.25-4.81, P=0.009], in addition to American Joint Committee on Cancer(AJCC) stage(P=0.010) and tumor budding(P=0.009).Conclusions: Parasympathetic neurogenesis is strongly associated with tumor budding and correlates with an adverse prognosis in PDAC. | Lingfu Zhang Limei Guo Ming Tao Wei Fu Dianrong Xiu | 2016 | Chinese Journal of Cancer Research2016，28，2： | 8 |
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