| 3 | Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis显示文摘Multiple sclerosis is characterized by demyelination and neuronal loss caused by inflammatory cell activation and infiltration into the central nervous system.Macrophage polarization plays an important role in the pathogenesis of experimental autoimmune encephalomyelitis,a traditional experimental model of multiple sclerosis.This study investigated the effect of Fasudil on macrophages and examined the therapeutic potential of Fasudil-modified macrophages in experimental autoimmune encephalomyelitis.We found that Fasudil induced the conversion of macrophages from the pro-inflammatory M1 type to the anti-inflammatory M2 type,as shown by reduced expression of inducible nitric oxide synthase/nitric oxide,interleukin-12,and CD16/32 and increased expression of arginase-1,interleukin-10,CD14,and CD206,which was linked to inhibition of Rho kinase activity,decreased expression of toll-like receptors,nuclear factor-κB,and components of the mitogen-activated protein kinase signaling pathway,and generation of the pro-inflammatory cytokines tumor necrosis factor-α,interleukin-1β,and interleukin-6.Crucially,Fasudil-modified macrophages effectively decreased the impact of experimental autoimmune encephalomyelitis,resulting in later onset of disease,lower symptom scores,less weight loss,and reduced demyelination compared with unmodified macrophages.In addition,Fasudil-modified macrophages decreased interleukin-17 expression on CD4^(+)T cells and CD16/32,inducible nitric oxide synthase,and interleukin-12 expression on F4/80^(+)macrophages,as well as increasing interleukin-10 expression on CD4^(+)T cells and arginase-1,CD206,and interleukin-10 expression on F4/80^(+)macrophages,which improved immune regulation and reduced inflammation.These findings suggest that Fasudil-modified macrophages may help treat experimental autoimmune encephalomyelitis by inducing M2 macrophage polarization and inhibiting the inflammatory response,thereby providing new insight into cell immunotherapy for multiple sclerosis. | Chunyun Liu Shangde Guo Rong Liu Minfang Guo Qing Wang Zhi Chai Baoguo Xiao Cungen Ma | 2024 | Neural Regeneration Research2024，19，3： | 0 |
| 4 | Study on the Form Factor Estimation Method of High-Speed Catamaran with Asymmetrical Hulls显示文摘The method used to estimate the form factor of low-speed vessel will cause a large error when estimating the form factor of high-speed catamaran because of the interference effects. A method based on computational fluid dynamics( CFD) method is proposed to estimate the form factor of high-speed catamaran with asymmetrical hulls. This paper focused on a 2000-toners catamaran with asymmetrical hulls to compare the difference between normal method and CFD method. The resistance of this catamaran is calculated by the CFD method,and it was compared to the model test data to verify the validity of this method. The form factors calculated by CFD method are very different from the results calculated by Prohaska method in high speed area.Thus,the method used to estimate the form factor of low-speed vessel is not applicative for high-speed catamaran. It is more accurate and efficient when using the CFD method to estimate the form factor of high-speed catamaran with asymmetrical hulls. | Chongjian Zhou Cungen Liu Xuefeng Wang Zelin Dai | 2016 | Journal of Harbin Institute of Technology(New Series)2016，23，4： | 0 |
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