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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | The herbal compound geniposide rescues formaldehyde-induced apoptosis in N2a neuroblastoma cells显示文摘The herbal medicine Tong Luo Jiu Nao(TLJN)contains geniposide(GP)and ginsenoside Rg1 at a molar ratio of 10:1.Rg1 is the major component of another herbal medicine,panax notoginseng saponin(PNS).TLJN has been shown to strengthen brain function in humans,and in animals it improves learning and memory.We have previously shown that TLJN reduces amyloidogenic processing in Alzheimer’s disease(AD)mouse models.Together this suggests TLJN may be a potential treatment for patients with dementia.Because chronic damage of the central nervous system by formaldehyde(FA)has been presented as a risk factor for age-associated cognitive dysfunction,in the present study we investigated the protective effect of both TLJN and GP in neuron-like cells exposed to FA.FA-exposed murine N2a neuroblastoma cells were incubated with TLJN,its main ingredient GP,as well as PNS,to measure cell viability and morphology,the rate of apoptosis and expression of genes encoding Akt,FOXO3,Bcl2 and p53.The CCK-8 assay,cytoskeletal staining and flow cytometry were used to test cell viability,morphology and apoptosis,respectively.Fluorescent quantitative real-time PCR(qRT-PCR)was used to monitor changes in gene expression,and HPLC to determine the rate of FA clearance.Treatment of N2a cells with 0.09 mmol L?1 FA for 24 h significantly reduced cell viability,changed cell morphology and promoted apoptosis.Both TLJN and GP conferred neuroprotection to FA-treated N2a cells,whereas PNS,which had to be used at lower concentrations because of its toxicity,did not.Our data demonstrate that TLJN can rescue neuronal damage caused by FA and that its main ingredient,GP,has a major role in this efficacy.This presents purified GP as a drug or lead compound for the treatment of AD. | CHEN JinYan SUN MengRu WANG XingHua LU Jing WEI Yan TAN Yan LIU Ying GTZ Jürgen HE RongQiao HUA Qian | 2014 | Science China(Life Sciences)2014，57，4： | 15 |
| 3 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 4 | Variation of summer monsoon intensity on the Loess Plateau of Central China during the last 130 000 a——Evidence from Rb and Sr distribution显示文摘THE loess-paleosol sequences from China potentially provide one of the best terrestrial records of past climates. Studies have shown that magnetic susceptibility in the sequences represents a proxy climate index closely related to changes of summer monsoon intensity. Some interpretative models have been advanced to account for these magnetic variations, although unanimity of opinion has not been reached. Recent geochemical studies of chemical | Chen, J Qiu, G Lu, HY Ji, JF | 1997 | Chinese Science Bulletin1997，42，6： | 4 |
| 5 | Low carbon steel with nanostructured surface layer induced by high-energy shot peening显示文摘 | G Liu S.C Wang X.F Lou J Lu K Lu | 2001 | Scripta Materialia2001，，8： | 3 |
| 6 | Low carbon steel with nanostructured surface layer induced by high-energy shot peening显示文摘 | G Liu S.C Wang X.F Lou J Lu K Lu | 2001 | Scripta Materialia2001，，8： | 2 |
| 7 | 显示文摘 | Linsebigler A Lu G Yates J T | 1995 | Chem Rev1995，95，： | 2 |
| 8 | Generating chaos with a switching piecewise-linear controller 显示文摘 | J Lu T S Zhou G Chen X S Yang | 2002 | Chaos2002，12，2： | 1 |
| 9 | Effects of moisture on properties of epoxy molding compounds 显示文摘 | LU M G SHIM M J KIM S W | 2001 | J Appl Polym Sci2001，81，： | 1 |
| 10 | Active cooling by metallicsandwich structures with periodic cores显示文摘 | Lu T J Valdevit L Evans A G | 2005 | Prog Mater Sci2005，50，： | 1 |
| 11 | 显示文摘 | Lu J G Ye Z Z Huang J Y | 2002 | Mater Sci SemiconProcess2002，5，： | 1 |
| 12 | Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials 显示文摘 | Lu L L Liu Y J Yang S G | 2006 | Haematologica2006，91，8： | 1 |
| 13 | Storm wave reactivation of a submarine landslide显示文摘 | Prior D B Suhayda J N Lu N J Bomhold B D Keller G H Wiseman W J Wright L D Yang Z S | 1989 | Nature1989，341，： | 1 |
| 14 | Second harmonic generation and self-frequency doubling performance in Nd: GdCa4O(BO3)3 crystal显示文摘 | LU J LI G LIU J | 1999 | Optics Communications1999，168，56： | 1 |
| 15 | Resveratrol analog,3,4,5,4'-tetrahydroxystilbene,differentially induces proapoptotic p53/Bax gene expression and inhibits the growth of transformed cells but not their normal counterparts显示文摘 | Lu J B Ho C T Ghai G | 2001 | Carcinogenesis2001，22，2： | 1 |
| 16 | Orientation analysis for rotated human face detection显示文摘 | Zhou J Lu X G Zhang D | 2002 | Image and Vision Compution2002，，20： | 1 |
| 17 | MicroRNA expression profiles classify human cancers显示文摘 | Lu J Getz G Miska E A | 2005 | Nature2005，435，7043： | 1 |
| 18 | Influence of silicon on the α-Fe/Γ interface of hot-dip galvanized steels显示文摘 | LU J T CHE C S KONG G FUMIO N | 2006 | Surface and Coating Technology2006，200，1819： | 1 |
| 19 | A New Chaotic Attractor Coined显示文摘 | LU J H CHEN G | 2002 | Int J of Bifurcation and Chaos2002，12，3： | 1 |
| 20 | MicroRNA expression profiles classify human cancers显示文摘 | Lu J Getz G Miska EA | 2005 | Nature2005，435，7043： | 1 |
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