|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Outbreak of Dengue Fever in Central China,2013显示文摘In 2013,the first dengue fever(DF) outbreak in central China was reported in the central of Henan province,northern temperate regions,although they have been sequentially recorded in Southern China.106 suspected DF cases were reported and 73 patients were confirmed dengue virus type 3(DEN-3) infections.62/392(15.8%) local health persons showed DEN antibodies positive.To this day Henan is the northernmost province in China which has been reported about outbreak of DF and what is important is that it warns us the endemic range of DF has been expanded geographically in China. | HUANG Xue Yong MA Hong Xia WANG Hai Feng DU Yan Hua SU Jia LI Xing Le TANG Xiao Yan MA Hong Peng ZU Bing Can ZHANG Qiao Hong CHEN Hao Min XU Bian Li | 2014 | Biomedical and Environmental Sciences2014，27，11： | 30 |
| 2 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 3 | ABO sequence analysis in an AB type with anti-B patient显示文摘ABO blood group system is the first to be discovered and the most clinically important in transfusion medicine.1The ABO gene encodes a glycosyltransferase which synthesize the oligosaccharide epitopes.The difference between the consensus A gene (A101 allele) usually used as the reference and the consensus B gene (B101 allele) are 7 in-frame single nucleotide polymorphisms (SNPs),among which only 4 alleles cause amino acid mutations in the enzyme.When A allele was inherited from one parent and B allele was inherited from the other which produced the AB blood group,this is known as trans-AB.Cis-AB is characterized as mutant ABO alleles encoding a glycosyltransferase capable of synthesizing both A and B antigens which is very rare. | Chen Qing Xiao Jianyu Wang Shuya Li Jiahuang Du Leilei Lu Le Huang Chengyin Li Min Li Ping | 2014 | Chinese Medical Journal2014，，5： | 17 |
| 4 | Unique cytologic features of thyroiditis caused by immune checkpoint inhibitor therapy for malignant melanoma显示文摘Blockade of immune checkpoint molecules to reverse cancer-induced immune suppression can improve anti-tumor immune responses in cancer patients.Monoclonal antibodies targeting two such molecules,Programmed cell death protein 1(PD-1)and cytotoxic T-lymphocyte associated protein 4(CTLA-4)have shown clinical benefit in the treatment of advanced malignancies,including metastatic melanoma.Adverse effects of these immune checkpoint inhibitors include immune-related adverse events(irAE),of which one of the most common is autoimmune thyroiditis.Though thyroiditis is increasingly recognized,there are no reports of the pathological findings that occur in immunotherapy-induced thyroiditis.We present a case of immunotherapy-induced thyroiditis demonstrating its unique cytopathologic features.A 51-year-old woman with metastatic melanoma was found to have a suppressed TSH and elevated free thyroxine concentration 14 days after starting treatment with nivolumab(PD-1 antagonist)plus ipilimumab(CTLA-4 antagonist)therapy.A thyroid biopsy was performed based on ultrasound findings and cytopathology revealed unique features including abundant clusters of necrotic cells,lymphocytes and CD163-positive histiocytes.This case reports cytopathologic features found in immune checkpoint inhibitor related thyroiditis.These appear to be unique findings and may help inform future research regarding the pathophysiology and mechanisms of this condition. | Trevor E.Angell Le Min Tad J.Wieczorek F.Stephen Hodi | 2018 | Genes & Diseases2018，5，1： | 13 |
| 5 | Validation of the chinese version of the EORTC QLQ-CR29 in patients with colorectal cancer显示文摘AIM To assess the validity and reliability of the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Colorectal Cancer 29(EORTC QLQ-CR29) in Chinese patients with colorectal cancer(CRC). METHODS From March 2014 to January 2015, 356 patients with CRC from four different hospitals in China were enrolled in the study, and all patients self-administered the EORTC QLQ-CR29 and the quality of life core questionnaire(EORTC QLQ-C30). Evaluation of the scores was based on the Karnofsky Performance Scale(KPS). The reliability and validity of the questionnaires were assessed by Cronbach's α coefficient, the Spearman correlation test and Wilcoxon rank sum test.RESULTS The EORTC QLQ-CR29 showed satisfactory reliability(α > 0.7), although the urinary frequency and blood and mucus in stool dimensions had only moderate reliability(α = 0.608). The multitrait scaling analyses showed good convergent(r > 0.4) and discriminant validity. Significant differences were obtained for each item in the different KPS subgroups(KPS ≤ 80; KPS > 80). body image and most single-item dimensions showed statistically significant differences in patients with a stoma compared with the rest of the patients. CONCLUSION The EORTC QLQ-CR29 exhibits high validity and reliability in Chinese patients with CRC, and can therefore be recommended as a valuable tool for the assessment of quality of life in these patients. | Jin-bo Lin Lei Zhang Dong-Wen Wu Zhou-Huan xi xue-Jun Wang Yun-Shou Lin Wakana Fujiwara Jing-Ru Tian Min Wang Peng Peng Ai Guo Zhen Yang Le Luo Ling-Ya Jiang Qia-Qia Li xue-Ying Zhang Yun-Feng Zhang Hou-Wei xu bing Yang xun-Lin Li Yi-xiong Lei | 2017 | World Journal of Gastroenterology2017，23，10： | 12 |
| 6 | Reversal of idiopathic hypogonadotropic hypogonadism: a cohort study in Chinese patients显示文摘尽管自发的 hypogonadotropic 性腺机能减退(IHH ) 传统地被看作了释放 gonadotropin 荷尔蒙神经原的缺乏引起的终生的疾病,病人的部分可以逐渐地在神经质的代替治疗期间重获正常的繁殖的轴功能。为潜在的 IHH 颠倒的预兆的因素大部分是未知的。我们的学习的目的是调查发生和颠倒了繁殖的轴功能的 IHH 男病人的临床的特征。在这回顾的队研究,男 IHH 病人被分类进一个颠倒组(n = 18 ) 并且一个 nonreversal 组(n = 336 ) 。gonadotropins 和睾丸激素的集中,以及睾丸尺寸和精子数,被决定。354 个 IHH 病人,(5.1%) 18 在治疗期间获得了正常繁殖功能。为颠倒的中部的年龄是 24 岁(范围 21-34 年) 。与 nonreversal 组相比,可逆的组有更高基础的 luteinizing 荷尔蒙(LH )(1.0 ±0.7 IU l -1 对 0.4 ±0.4 IU l −1, P <0.05 ) 并且刺激 LH (28.3 ±22.6 IU l −1 对 1.9 ±1.1 IU l −1, P <0.01 ) 层次,以及更大的睾丸尺寸(5.1 ±2.6 ml 对 1.5 ±0.3 ml, P <0.01 ) ,在起始的访问。在摘要,更大的睾丸尺寸和更高刺激的 LH 集中是为颠倒的喜爱的参数。我们发现建议可逆病人可以在起始的诊断保留部分活跃的繁殖的轴功能。 | Jiang-Feng Mao Hong-Li Xu Jin Duan Rong-Rong Chen Li Li Bin Li Min Nie Le Min Hong-Bing Zhang Xue-Yan Wu | 2015 | Asian Journal of Andrology2015，17，3： | 9 |
| 7 | Regulation of Rice Tillering by RNA-Directed DNA Methylation at Miniature Inverted-Repeat Transposable Elements显示文摘Tillering is a major determinant of rice plant architecture and grain yield.Here,we report that depletion of rice OsNRPD1a and OsNRPD1b,two orthologs of the largest subunit of RNA polymerase IV,leads to a high-tillering phenotype,in addition to dwarfism and smaller panicles.OsNRPD1a and OsNRPD1b are required for the production of 24-nt small interfering RNAs that direct DNA methylation at transposable elements(TEs)including miniature inverted-repeat TEs(MITEs).Interestingly,many genes are regulated either positively or negatively by TE methylation.Among them,OsMIR156d and OsMIR156j,which promote rice tillering,are repressed by CHH methylation at two MITEs in the promoters.By contrast,D14,which suppresses rice tillering,is activated by CHH methylation at an MITE in its downstream.Our findings reveal regulation of rice tillering by RNA-directed DNA methylation at MITEs and provide potential targets for agronomic trait enhancement through epigenome editing. | Le Xu Kun Yuan Meng Yuan Xiangbing Meng Min Chen Jianguo Wu Jiayang Li Yijun Qi | 2020 | Molecular Plant2020，13，6： | 9 |
| 8 | Pulsatile gonadotropin-releasing hormone therapy is associated with earlier spermatogenesis compared to combined gonadotropin therapy in patients with congenital hypogonadotropic hypogonadism显示文摘 | Jiang-Feng Mao Zhao-Xiang Liu Min Nie Xi Wang Hong-Li Xu Bing-Kun Huang Jun-Jie Zheng Le Min Ursula Brigitte Kaiser Xue-Yan Wu | 2017 | Asian Journal of Andrology2017，19，6： | 8 |
| 9 | Assessment of the cropland classifications in four global land cover datasets: A case study of Shaanxi Province, China显示文摘Accurate and reliable cropland surface information is of vital importance for agricultural planning and food security monitoring. As several global land cover datasets have been independently released,an inter-comparison of these data products on the classification of cropland is highly needed. This paper presents an assessment of cropland classifications in four global land cover datasets,i.e.,moderate resolution imaging spectrometer(MODIS) land cover product,global land cover map of 2009(Glob Cover2009),finer resolution observation and monitoring of global cropland(FROM-GC) and 30-m global land cover dataset(Globe Land30). The temporal coverage of these four datasets are circa 2010. One of the typical agricultural regions of China,Shaanxi Province,was selected as the study area. The assessment proceeded from three aspects: accuracy,spatial agreement and absolute area. In accuracy assessment,506 validation samples,which consist of 168 cropland samples and 338 non-cropland ones,were automatically and systematically selected,and manually interpreted by referencing high-resolution images dated from 2009 to 2011 on Google Earth. The results show that the overall accuracy(OA) of four datasets ranges from 61.26 to 80.63%. Globe Land30 dataset,with the highest accuracy,is the most accurate dataset for cropland classification. The cropland spatial agreement(mainly located in the plain ecotope of Shaanxi) and the non-cropland spatial agreement(sparsely distributed in the south and middle of Shaanxi) of the four datasets only makes up 33.96% of the whole province. FROM-GC and Globe Land30,obtaining the highest spatial agreement index of 62.40%,have the highest degree of spatial consistency. In terms of the absolute area,MODIS underestimates the cropland area,while Glob Cover2009 significantly overestimates it. These findings are of value in revealing to which extent and on which aspect that these global land cover datasets may agree with each other at small scale on each ecotope region. The approaches taken in this study could be used to derive a fused cropland classification dataset. | CHEN Xiao-yu LIN Ya ZHANG Min YU Le LI Hao-chuan BAI Yu-qi | 2017 | Journal of Integrative Agriculture2017，16，2： | 8 |
| 10 | Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis显示文摘在 Arabidopsis 的有气孔的系和随后的不对称的部门的开始要求基本 helix-loop-helix 抄写因素的活动哑(SPCH ) 。SPCH 也激活 MITOGEN 的蛋白质 KINASE (MAPK ) 串联或象 GSK3 一样 kinase BRASSINOSTEROID 作为底层控制入口进有气孔的系,这被显示出感觉迟钝 2 (BIN2 ) 。这里,我们证明 SPCH 的三丝氨酸残余看起来是 Cyclin 依赖的 Kinases A 的主要 phosphorylation 目标; 1 (CDKA; 1 ) invitro,并且在他们之中,丝氨酸 186 在有气孔的形成起一个关键作用。怀有在丝氨酸 186 残余上导致 phosphorylation 缺乏的一个变化的 SPCH 构造的表示没能在 spch 0 异种救有气孔的缺点。phosphorylation 模仿异种 SPCH S186D 在怀有主导否定的 CDKA 的指向的表示的转基因的线补充了有气孔的生产缺点; 1 .N146。除了 SPCH 上的调停 MAPK 、调停 BIN2 的 phosphorylation,因此,在丝氨酸 186 点的 phosphorylation 断然在调整有气孔的开发为 SPCH 功能被要求。 | Ke-Zhen Yang Min Jiang Ming Wang Shan Xue Ling-Ling Zhu Hong-Zhe Wang Jun-Jie Zou Eun-Kyoung Lee Fred Sack Jie Le | 2015 | Molecular Plant2015，8，5： | 7 |
| 11 | Comprehensive evaluation on the ecological function of groundwater in the Shiyang River watershed显示文摘With an arid climate and shortage of water resources,the groundwater dependent ecosystems in the oasis-desert ecotone of the Shiyang River Watershed has been extremely damaged,and the water crisis in the oasis has become a major concern in the social and the scientific community.In this study,the degene-ration characteristics of the groundwater ecological function was identified and comprehensive evaluated,based on groundwater depth data,vegetation quadrat and normalized difference vegetation index(NDVI)from Landsat program.The results showed that(1)the suitable groundwater depth for sustainable ecology in the Shiyang River Watershed is about 2-4 m;(2)the terms of degenerative,qualitative and disastrous stages of the groundwater ecological function are defined with the groundwater depths of about 5 m,7 m and 10 m;(3)generally,the groundwater ecological function in the oasis-desert ecotone of the lower reaches of Shiyang River Watershed is weak with an area of 1397.9 km2 identified as the severe deterioration region,which accounted 74.7%of the total area.In the meantime,the percentages of the good,mild and moderate deterioration areas of groundwater ecological function are 3.5%,5.5%and 16.3%,respectively,which were mainly distributed in the Qingtu lake area and the southeastern area of the Shoucheng town;(4)the degradation and shrinkage of natural oasis could be attributed to the dramatic groundwater decline,which is generally caused by irrational use of water and soil resources.This study could provide theoretical basis and scientific support for the decision-making in environmental management and ecological restoration of the Shiyang River Watershed. | Min Liu Zhen-long Nie Le Cao Li-fang Wang Hui-xiong Lu Zhe Wang Pu-cheng Zhu | 2021 | Journal of Groundwater Science and Engineering2021，9，4： | 5 |
| 12 | Novel lignans from the stems and leaves of Trachelospermum jasminoides显示文摘5-methoxydibenzylhydroxybutyrolactone lignans 说出 5-methoxytracheloside 和 5-methoxytrachelogenin 的二篇小说从 Trachelospermum jasminoides ( Lindl )的茎和叶子被孤立 Lem ,他们的结构是被决定( 8S , 8S )-8-hydroxy-3, 3 , 4 , 5-tertramethoxylignan-9 , 9-olide-4-O--d-glucopyranoside 并且( 8S , 8S )-4, 8-dihydroxy-3 , 3 , 4 , 5-tertramethoxylignan-9 ,由各种各样的分光镜的分析的 9-olide 。5-Methoxytracheloside 是新混合物, 5-methoxytrachelogenin 第一次从生来的来源被孤立。 | Ling Jing Neng Jiang Yu Yu Shan Li Le Fu Yi Min Zhao | 2011 | Chinese Chemical Letters2011，22，9： | 5 |
| 13 | Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish显示文摘到许多蛋白质的小 ubiquitin 相关的修饰词(相扑) 变化形式调整多样的细胞的过程,包括抄写,房间周期规定和染色体正直的维护。在 vivo 调查相扑 paralogs 的生物功能,我们在 zebrafish 的早开发使他们失去活性。当作为 Ubc9 缺乏的,为所有三相扑 paralogs 缺乏的 zebrafish 胚胎显示了严重缺点时,单个相扑 paralog 的损失与正常开发兼容。相扑缺乏的胚胎能被一个单个人或 zebrafish 相扑救。当关键时结构的基本离氨酸残余和 N 终端相扑的未组织的段是批评的为在 vivo 营救, SUMO2 的一致 K11 sumoylation 地点是非必需的,在这个潜在的地点上暗示那链形成为正常开发是非本质的。所有三相扑的 Inactivation 触发了 p53 依赖的 apoptosis, p53 的进一步的 inactivation 恢复了正常 zebrafish 开发。有趣地,我们也证明 p53 的主导的否定截断的形式,螖 1 13p53,显著地弄钝相扑在 vivo 的导致弄空的 p53 活动。一起拿,我们的结果建议相扑 paralogs 不可缺少,却冗余,在 zebrafish 的早发展。 | Hao Yuan Jun Zhou Min Deng Xi Liu Morgane Le Bras Hugues de The Sai Juan Chen Zhu Chen Ting Xi Liu Jun Zhu | 2010 | Cell Research2010，20，2： | 4 |
| 14 | The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),a member of the Phlebovirus genus from the Bunyaviridae family endemic to China,is the causative agent of life-threatening severe fever with thrombocyto-penia syndrome(SFTS),which features high fever and hemorrhage.Similar to other negative-sense RNA viruses,SFTSV encodes a nucleocapsid protein(NP)that is essen-tial for viral replication.NP facilitates viral RNA encapsida-tion and is responsible for the formation of ribonucleopro-tein complex.However,recent studies have indicated that NP from Phlebovirus members behaves in inhomogene-ous oligomerization states.In the present study,we report the crystal structure of SFTSV NP at 2.8Åresolution and demonstrate the mechanism by which it processes a ring-shaped hexameric form to accomplish RNA encapsida-tion.Key residues essential for oligomerization are identi-fi ed through mutational analysis and identifi ed to have a signifi cant impact on RNA binding,which suggests that correct formation of highly ordered oligomers is a criti-cal step in RNA encapsidation.The fi ndings of this work provide new insights into the discovery of new antiviral reagents for Phlebovirus infection. | Honggang Zhou Yuna Sun Ying Wang Min Liu Chao Liu Wenming Wang Xiang Liu Le Li Fei Deng Hualin Wang Yu Guo Zhiyong Lou | 2013 | Protein & Cell2013，4，6： | 4 |
| 15 | A new loss-of-function allele 28y reveals a role of ARGONAUTE1 in limiting asymmetric division of stomatal lineage ground cell显示文摘In Arabidopsis thaliana L., stomata are produced through a series of divisions including asymmetric and symmetric divisions. Asymmetric entry division of meristemoid mother cell produces two daughter cells, the smaller meristemoid and the larger sister cell, a stomatal lineage ground cell(SLGC). Stomatal lineage ground cells can differentiate into epidermal pavement cells but have the potential to divide asymmetrically, spacing divisions, to create satellite meristemoids. Peptide ligands and TOO MANY MOUTHS(TMM) and ERECTA family receptors regulate the initiation of stomatal lineages, activity, and orientation of spacing divisions. Here, we reported that a natural mutant 28 y displayed an increased stomatal density and index. Using map-based cloning, we identified mutation in ARGONAUTE1(AGO1)as the cause of 28 y phenotypes. Time-lapse tracing of stomatal lineage cells reveals that stomatal overproduction in 28 y is caused by the excessive asymmetric spacing division of SLGCs.Further genetic results demonstrated that AGO1 acts downstream of TMM and negatively regulates the SPCH transcripts,but in a brassinosteroid-independent manner. Upregulation of AGAMOUS-LIKE16(AGL16) in 28 y mutants suggests that AGO1 is required to restrict AGL16-mediated stomatal spacing divisions,an miRNA pathway in addition to ligand-receptor signaling modules. | Kezhen Yangy Min Jiangy Jie Le | 2014 | Journal of Integrative Plant Biology2014，56，6： | 3 |
| 16 | Immune-related endocrine disorders in novel immune checkpoint inhibition therapy显示文摘Immune checkpoint inhibition against advance malignancies was named breakthrough discovery by the science magazine in 2013.In numerous clinical studies,monoclonal antibodies against the immune checkpoints,CTLA4,PD1 and PD1 ligand PDL1 have shown promising tumor response in different type of metastatic malignancies.The adverse events are autoimmune-related.The endocrine disorders,hypophysitis and thyroiditis are among the most common side effects associated with immune checkpoint inhibition treatment.Hypophysitis,a very rare endocrine disorder occurs in about one tenth of the patients receiving anti-CTLA4 treatment.Thyroiditis,on the other hand,is more commonly seen in patients receiving anti-PD1 treatment.In addition,both thyroiditis and hypophysitis are common in patients receiving combination treatment with anti-CTLA4 and anti-PD1 treatment.The time to onset of hypophysitis and thyroiditis is short.Most of the endocrine disorders occur within 12 weeks after initiation of the immune checkpoint inhibition therapy.Hypophysitis can manifest as total anterior pituitary hormone deficiency or isolated pituitary hormone deficiency.Diabetes insipidus is rare.TSH and gonadotropin deficiencies may be reversible but ACTH deficiency appears permanent.Thyroiditis can present as hypothyroidism or thyrotoxicosis followed by hypothyroidism.Hypothyroidism appears irreversible.Early identifying the onset of hypophysitis and thyroiditis and proper management of these endocrine disorders will improve the quality of the life and the outcome of this novel immunotherapy. | Le Min | 2016 | Genes & Diseases2016，3，4： | 3 |
| 17 | Mitochondrial Oxidative Stress Enhances Vasoconstriction by Altering Calcium Homeostasis in Cerebrovascular Smooth Muscle Cells under Simulated Microgravity显示文摘Objective Exposure to microgravity results in postflight cardiovascular deconditioning in astronauts.Vascular oxidative stress injury and mitochondrial dysfunction have been reported during this process.To elucidate the mechanism for this condition,we investigated whether mitochondrial oxidative stress regulates calcium homeostasis and vasoconstriction in hindlimb unweighted(HU)rat cerebral arteries.Methods Three-week HU was used to simulate microgravity in rats.The contractile responses to vasoconstrictors,mitochondrial fission/fusion,Ca^(2+) distribution,inositol 1,4,5-trisphosphate receptor(IP3 R)abundance,and the activities of voltage-gated K+channels(KV)and Ca^(2+)-activated K+channels(BKCa)were examined in rat cerebral vascular smooth muscle cells(VSMCs).Results An increase of cytoplasmic Ca^(2+) and a decrease of mitochondrial/sarcoplasmic reticulum(SR)Ca^(2+) were observed in HU rat cerebral VSMCs.The abundance of fusion proteins(mitofusin 1/2[MFN1/2])and fission proteins(dynamin-related protein 1[DRP1]and fission-mitochondrial 1[FIS1])was significantly downregulated and upregulated,respectively in HU rat cerebral VSMCs.The cerebrovascular contractile responses to vasoconstrictors were enhanced in HU rats compared to control rats,and IP3 R protein/mRNA levels were significantly upregulated.The current densities and open probabilities of KV and BKCa decreased and increased,respectively.Treatment with the mitochondrial-targeted antioxidant mitoTEMPO attenuated mitochondrial fission by upregulating MFN1/2 and downregulating DRP1/FIS1.It also decreased IP3 R expression levels and restored the activities of the KV and BKCa channels.MitoTEMPO restored the Ca^(2+) distribution in VSMCs and attenuated the enhanced vasoconstriction in HU rat cerebral arteries.Conclusion The present results suggest that mitochondrial oxidative stress enhances cerebral vasoconstriction by regulating calcium homeostasis during simulated microgravity. | LIU Zi Fan WANG Hai Ming JIANG Min WANG Lin LIN Le Jian ZHAO Yun Zhang SHAO Jun Jie ZHOU Jing Jing XIE Man Jiang LI Xin ZHANG Ran | 2021 | Biomedical and Environmental Sciences2021，34，3： | 3 |
| 18 | Anti-oxidant and anti-inflammatory activities of ultrasonicassistant extracted polyphenol-rich compounds from Sargassum muticum显示文摘Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances. | YU Yuan WANG Le FU Xiaoting WANG Lei FU Xiaodan YANG Min HAN Zhenlian MOU Haijin JEON You-Jin | 2019 | Journal of Oceanology and Limnology2019，37，3： | 3 |
| 19 | Influences of 3-methylcholanthrene,phenobarbital and dexamethasone on xenobiotic metabolizing-related cytochrome P450 enzymes and steroidogenesis in human fetal adrenal cortical cells显示文摘瞄准:在胎儿的开发期间在肾的 steroidogenesis 上探索影响和 xenobiotics 的可能的机制。方法:主要人的胎儿的肾的外皮的房间与 3-methylcholanthrene,苯巴比妥和地塞米松被准备、有教养、对待。7-ethoxyresorufin O-dealkylase, benzphetamine,一种无色透明化合物和抗生素的一种 N-demethylases 的活动被酶试金测量。同时,类固醇荷尔蒙皮质醇,醛固酮,睾丸激素和孕酮的定量分析被放射性免疫测定在文化媒介执行。结果:benzphetamine 和一种无色透明化合物 N-demethylase 的活动在为 24 h 与苯巴比妥(0.25-1 mmol/L ) 对待的文化胎儿的肾的房间被增加。地塞米松(25-100 micromol/L ) 也增加了抗生素的一种 N-demethylase 的活动。7-ethoxyresorufin O-dealkylase 的活动在没有并且与 3-methylcholanthrene (0.5-2 micromol/L ) ,对待的房间是未被发现的。同时,中等皮质醇,醛固酮和孕酮的内容是有 3-methylcholanthrene 的减少的术后疗法。皮质醇,醛固酮和孕酮集中稍微也与苯巴比妥被减少。地塞米松显著地提高了皮质醇和孕酮的生产。睾丸激素集中的趋势在 3-methylcholanthrene,苯巴比妥或地塞米松处理以后是不明确的。结论:3-Methylcholanthrene,苯巴比妥或地塞米松能在主要人的胎儿的肾的外皮的房间防碍皮质醇,醛固酮和孕酮的合成,它多半通过 xenobiotic 扮演使相关产生代射变化的细胞色素 P450 对碘氧基苯甲醚激活。 | Hui WANG Min HUANG Ren-xiu PENG Jiang LE | 2006 | Acta Pharmacologica Sinica2006，27，8： | 2 |
| 20 | Skp2 dictates cell cycle-dependent metabolic oscillation between glycolysis and TCA cycle显示文摘Whether glucose is predomi nantly metabolized via oxidative phosphorylati on or glycolysis differs between quiesce nt versus proliferating cells,iincluding tumor cells.However,how glucose metabolism is coordinated with cell cycle in mammalian cells remains elusive.Here,we report that mammalian cells predominantly utilize the tricarboxylic acid(TCA)cycle in G1 phase,but prefer glycolysis in S phase.Mechanistically,coupling cell cycle with metabolism is largely achieved by timely destruction of IDH1/2,key TCA cycle enzymes,in a Skp2-dependent manner.As such,depleting SKP2 abolishes cell cycle-dependent fluctuation of IDH1 protein abundance,leading to reduced glycolysis in S phase.Furthermore,elevated Skp2 abundance in prostate cancer cells destabilizes IDH1 to favor glycolysis and subsequent tumorigenesis.Therefore,our study reveals a mechanistic link between two cancer hallmarks,aberrant cell cycle and addiction to glycolysis,and provides the underlying mechanism for the coupling of metabolic fluctuation with periodic cell cycle in mammalian cells. | Jing Liu Yunhua Peng Le Shi Lixin Wan Hiroyuki Inuzuka Jiangang Long Jianping Guo Jinfang Zhang Min Yuan Shuangxi Zhang Xun Wang Jing Gao Xiangpeng Dai Shozo Furumoto Lijun Jia Pier Paolo Pandolfic John M.Asara William G.Kaelin Jr. Jjankang Liu Wenyi Wei | 2021 | Cell Research2021，31，1： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员