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| 1 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 2 | High power and stable P-doped yolk-shell structured Si@C anode simultaneously enhancing conductivity and Li^(+)diffusion kinetics显示文摘Silicon is a low price and high capacity ancxje material for lithium-ion batteries.The yolk-shell structure can effectively accommodate Si expansion to improve stability.However,the limited rate performance of Si anodes can't meet people's growing demand for high power density.Herein,the phosphorus-doped yolk-shell Si@C materials(P-doped Si@C)were prepared through carbon coating on P-doped Si/SiO_(x)matrix to obtain high power and stable devices.Therefore,the as-prepared P-doped Si@C electrodes delivered a rapid increase in Coulombic efficiency from 74.4%to 99.6%after only 6 cycles,high capacity retention of-95%over 800 cycles at 4 A·g^(-1),and great rate capability(510 mAh·g^(-1)at 35 A·g^(-1)).As a result,P-doped Si@C anodes paired with commercial activated carbon and LiFePO_(4)cathode to assemble lithium-ion capacitor(high power density of〜61,080 W·kg^(-1)at 20 A·g^(-1))and lithium-ion full cell(good rate performance with 68.3 mAh·g^(-1)at 5 C),respectively.This work can provide an effective way tofurther improve power density and stability for energy storage devices. | Ming Chen Qinnan Zhou Jiantao Zai Asma Iqbal TsegayeTadesse Tsega Boxu Dong Xuejiao Liu Yuchi Zhang Changyu Yan Liang Zhao Ali Nazakat SharelPeisan E CheeTongJohn Low Xuefeng Qian | 2021 | Nano Research2021，14，4： | 5 |
| 3 | Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation显示文摘 | Li Hongshi Gu Zexu Wu Li'an Xia Liang Zhou Kecheng E Lingling Wang Dongsheng Kou Junping Liu Hongchen | 2014 | Chinese Medical Journal2014，，20： | 4 |
| 4 | Crystal structure and nucleotide selectivity of human IFIT5/ISG58显示文摘 | Feng Feng LingminYuan Yao E Wang Christopher Crowley Zongyang Lv Jingjing Li Yingfang Liu Genhong Cheng Su Zeng Huanhuan Liang | 2013 | Cell Research2013，23，8： | 4 |
| 5 | A Novel Model of Predicting Archie's Cementation Factor from Nuclear Magnetic Resonance(NMR) Logs in Low Permeability Reservoirs显示文摘The resistivity experimental measurements of core samples drilled from low permeability reservoirs of Ordos Basin,Northwest China,illustrate that the cementation factors are not agminate,but vary from 1.335 to 1.749.This leads to a challenge for the estimation of water and hydrocarbon saturation.Based on the analysis of Purcell equation and assumption that rock resistivity is determined by the parallel connection of numerous capillary resistances,a theoretical expression of cementation factor in terms of porosity and permeability is established.Then,cementation factor can be calculated if the parameters of porosity and permeability are determined.In the field application,porosity can be easily obtained by conventional logs.However,it is a tough challenge to estimate permeability due to the strong heterogeneity of low permeability reservoirs.Thus,the Schlumberger Doll Research(SDR)model derived from NMR logs has been proposed to estimate permeability.Based on the analysis of the theoretical expressions of cementation factor and SDR model,a novel cementation factor prediction model,which is relevant to porosity and logarithmic mean of NMR T2spectrum(T2lm),is derived.The advantage of this model is that all the input information can be acquired from NMR logs accurately.In order to confirm the credibility of the novel model,the resistivity and corresponding laboratory NMR measurements of 27 core samples are conducted.The credibility of the model is confirmed by comparing the predicted cementation factors with the core analyzed results.The absolute errors for all core samples are lower than 0.071.Once this model is extended to field application,the accuracy of water and hydrocarbon saturation estimation will be significantly improved. | Liang Wang Zhiqiang Mao Yujiang Shi Qin'e Tao Yumei Cheng Yong Song | 2014 | Journal of Earth Science2014，25，1： | 4 |
| 6 | Differently sized cuckoos pose different threats to hosts显示文摘Hole-nesting tits Parus spp.have been classified as'unsuitable'hosts for cuckoo parasitism because cuckoos cannot enter a cavity if the entrance is too small.However,Chinese tits could re-ject alien eggs and egg ejection rate increased with the local diversity of parasitic cuckoo species.Antiparasitic behavior among Chinese tits may have evolved due to greater size variation among sympatric cuckoo species.This raises the question of whether differently sized parasitic cuckoos pose different threats to Chinese tits.A green-backed tit Parus monticolus population that is sym-patric with Asian emerald cuckoo Chrysococcyx maculatus(eme-cuckoo,small-sized parasite)and common cuckoo Cuculus canorus(com-cuckoo,large-sized parasite),and a cinereous tit P.cinereus population that is only sympatric with com-cuckoo were chosen as study organisms.We observed behavioral response and recorded alarm calls of the 2 tit species to eme-cuckoo,com-cuckoo,chipmunk Tamias sibiricus(a nest predator)and dove Streptopelia orientalis(a harm-less control),and subsequently played back alarm calls to conspecific incubating females.In dummy experiments,both tit species performed intense response behavior to chipmunk,but rarely responded strongly to the 3 avian species.In playback experiments,both tit species responded strongly to conspecific chipmunk alarm calls,but rarely responded to dove alarm calls.The inten-sity of response of incubating female green-backed tits to eme-cuckoo and com-cuckoo alarm calls were similar to that of chipmunk alarm calls,while the intensity to eme-cuckoo alarm calls was higher than the intensity to dove alarm calls which was similar to that of com-cuckoo alarm calls.In contrast,few female cinereous tits responded to eme-cuckoo and com-cuckoo alarm calls.These findings indicated that the threat level of eme-cuckoo was slightly greater than that of com-cuckoo for sympatric green-backed tits,but not for allopatric cinereous tits. | Jiangping YU Mingju E Wei SUN Wei LIANG Haitao WANG Anders Pape MΦLLER | 2020 | Current Zoology2020，66，3： | 4 |
| 7 | Development and calibration of the Moon-based EUV camera for Chang'e-3显示文摘The process of development and calibration for the first Moon-based extreme ultraviolet(EUV) camera to observe Earth's plasmasphere is introduced and the design, test and calibration results are presented. The EUV camera is composed of a multilayer film mirror, a thin film filter, a photon-counting imaging detector, a mechanism that can adjust the direction in two dimensions, a protective cover, an electronic unit and a thermal control unit. The center wavelength of the EUV camera is 30.2 nm with a bandwidth of 4.6 nm. The field of view is 14.7° with an angular resolution of 0.08°, and the sensitivity of the camera is 0.11 count s-1Rayleigh-1. The geometric calibration, the absolute photometric calibration and the relative photometric calibration are carried out under different temperatures before launch to obtain a matrix that can correct geometric distortion and a matrix for relative photometric correction,which are used for in-orbit correction of the images to ensure their accuracy. | Bo Chen Ke-Fei Song Zhao-Hui Li Qing-Wen Wu Qi-Liang Ni Xiao-Dong Wang Jin-Jiang Xie Shi-Jie Liu Ling-Ping He Fei He Xiao-Guang Wang Bin Chen Hong-Ji Zhang Xiao-Dong Wang Hai-Feng Wang Xin Zheng Shu-Lin E Yong-Cheng Wang Tao Yu Liang Sun Jin-Ling Wang Zhi Wang Liang Yang Qing-Long Hu Ke Qiao Zhong-Su Wang Xian-Wei Yang Hai-Ming Bao Wen-Guang Liu Zhe Li Ya Chen Yang Gao Hui Sun Wen-Chang Chen | 2014 | Research in Astronomy and Astrophysics2014，14，12： | 4 |
| 8 | Outcomes of Medicare beneficiaries hospitalised with transient ischaemic attack and stratification using the ABCD^(2) score显示文摘Background Long-term outcomes for Medicare beneficiaries hospitalised with transient ischaemic attack(TIA)and role of ABCD^(2) score in identifying high-risk individuals are not studied.Methods We identified 40825 Medicare beneficiaries hospitalised from 2011 to 2014 for a TIA to a Get With The Guidelines(GWTG)-Stroke hospital and classified them using ABCD^(2)s of mortality and rehospitalisation(all-cause,ischaemic stroke,haemorrhagic stroke,myocardial infarction,and gastrointestinal and intracranial haemorrhage)for high-risk versus low-risk groups adjusted for patient and hospital characteristics.Results Of the 40825 patients,35118(86%)were high risk(ABCD^(2)≥4)and 5707(14%)were low risk(ABCD^(2)=0-3).Overall rate of mortality during 1-year follow-up after hospital discharge for the index TIA was 11.7%,44.3% were rehospitalised for any reason and 3.6%were readmitted due to stroke.Patients with ABCD^(2) score≥4 had higher mortality at 1 year than not(adjusted HR 1.18,95%CI 1.07 to 1.30).Adjusted risks for ischaemic stroke,all-cause readmission and mortality/all-cause readmission at 1 year were also significantly higher for patients with ABCD^(2) score≥4 vs 0-3.In contrast,haemorrhagic stroke,myocardial infarction,gastrointestinal bleeding and intracranial haemorrhage risk were not significantly different by ABCD^(2) score.Conclusions This study validates the use of ABCD^(2) score for long-term risk assessment after TIA in patients aged 65 years and older.Attentive efforts for community-based follow-up care after TIA are needed for ongoing prevention in Medicare beneficiaries who were hospitalised for TIA. | Shreyansh Shah Li Liang Durgesh Bhandary Saga Johansson Eric E Smith Deepak L Bhatt Gregg C Fonarow Naeem D Khan Eric Peterson Janet Prvu Bettger | 2021 | Stroke & Vascular Neurology2021，6，2： | 3 |
| 9 | Paleozoic oil–source rock correlations in the Tarim basin, NW China显示文摘 | S.C Zhang A.D Hanson J.M Moldowan S.A Graham D.G Liang E Chang F Fago | 2000 | Organic Geochemistry2000，，： | 3 |
| 10 | Frequent amplification of a chr19q13.41 microRNA polycistron in aggressive primitive neuroectodermal brain tumors显示文摘 | Li M Lee KF Lu Y Clarke I Shih D Eberhart C Collins VP Van Meter T Picard D Zhou L Boutros PC Modena P Liang ML Scherer SW Bouffet E Rutka JT Pomeroy SL Lau CC Taylor MD Gajjar A Dirks PB Hawkins CE Huang A. | 2009 | 中国神经肿瘤杂志2009，7，4： | 3 |
| 11 | Tracking of CFSE-labeled endothelial progenitor cells in laser-injured mouse retina显示文摘背景 Endothelial 祖先房间(EPC ) 移植是为 ischemic retinopathy 的有希望的治疗学的策略。当前的学习试图为与 5-(and-6 ) 追踪 EPC 建立一个简单、可靠、荧光灯的标记的方法在伤害激光的老鼠 retina.Methods EPC 的 -carboxyfluorescein diacetate succinimidyl 酉旨(CFSE ) 从人的脐的绳索血 mononuclear 房间被孤立,栽培,并且用 CFSE 的各种各样的集中标记。基于荧光紧张和房间形态学,有在 37 点的 5 mol/L CFSE 的 15 分钟孵化作为最佳的标记条件被选择。幸存能力和标记 CFSE 的 EPC 的 apoptosis 率被染色的 Trypan 蓝色和 Annexin V/PI 染色试金分别地测量。荧光显微镜学被用来在扩大文化时期期间观察标签稳定性。标记的 EPC 被移植进网膜的激光光致疑结伤害的 pigmented 老鼠的玻璃的洞。Evans 蓝 angiography 和完全装的视网膜被检验追踪用 5 mol/L CFSE 介绍了标记的标记的 cells.Results EPC 一块强烈草地荧光和维持的正常形态学没有在在被标记 2 天以后的幸存能力或 apoptosis 率的重要变化, 1 和 4 个星期。荧光紧张逐渐地在 4 个星期的结束在房间减少了。视网膜的 Evans 蓝 angiography 清楚地显示了网膜的毛细作用网络,荧光漏在伤害激光的老鼠模型在 photocoagulated 点附近被观察。在标记的 EPC 的移植以后的一个星期,荧光灯房间在 photocoagulated 损害附近被识别。在移植以后的四个星期,荧光灯像试管的结构在网膜的脉管的 networks.Conclusion EPC 被观察能被 CFSE 在 vitro 标记并且在 vivo 监视了至少 4 个星期,并且参予受伤网膜的容器的修理。 | SHI Hui YANG Wei CUI Zhi-hua LU Cheng-wei LI Xiao-hong LIANG Ling-ling SONG E | 2011 | Chinese Medical Journal2011，，5： | 3 |
| 12 | Discrete Particle Simulation of Gas-Solid Flow in Air-Blowing Seed Metering Device显示文摘In this paper,the gas and seed flow characters in the air-blowing seed metering device are investigated by using the coupled computational fluid dynamics and discrete element method(CFD-DEM)in three dimensions(3D).The method of establishing boundary model based on the computer-aided design(CAD)drawing,has been used to build the boundary model of seed metering device.The 3D laser scanning technique and multi-element method are adopted to establish the particle model.Through a combined numerical and experimental effort,using 3D CFD-DEM software,which is based on the in-house codes,the mechanisms governing the gas and solid dynamic behaviors in the seed metering device have been studied.The gas velocity field and the effect of different rotational speeds and air pressures on the seeding performance and particle velocity have been studied,similar agreements between numerical and experimental results are gained.This reveals that the 3D CFD-DEM model established is able to predict the performance of the air-blowing seed metering device.It can be used to design and optimize the air-blowing seed metering device and other similar agriculture devices. | Zhengquan Li Pei Zhang Yongchang Sun Chenglin Zheng Liang Xu Dianyu E | 2021 | Computer Modeling in Engineering & Sciences2021，，6： | 2 |
| 13 | 地中海膳食干预研究中来自胆碱通路的血浆代谢物与心血管病风险的关系显示文摘血浆甜菜碱水平以及胆碱代谢水平与主要心血管病终点之间的联系仍不清楚。研究者评估了胆碱通路的代谢物与偶发心血管病风险以及地中海膳食干预的潜在调节效应之间的相关性。 | 黄洁 叶鹏 Guasch-Ferré M Hu FB Ruiz-Canela M Bulló M Toledo E Wang DD Corella D Gómez-Gracia E Fiol M Estruch R Lapetra J Fitó M Arós F Serra-Majem L Ros E Dennis C Liang L Clish CB Martínez-González MA Salas-Salvadó J | 2017 | 中华高血压杂志2017，25，12： | 2 |
| 14 | Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows显示文摘Known as the‘third polar region’,the Qinghai-Tibet Plateau represents one of the harshest highland environments in the world and yet a number of organisms thrive there.Previous studies of birds,animals and humans have focused on well-differentiated populations in later stages of phenotypic divergence.The adaptive processes during the initial phase of highland adaptation remain poorly understood.We studied a human commensal,the Eurasian Tree Sparrow,which has followed human agriculture to the Qinghai-Tibet Plateau.Despite strong phenotypic differentiation at multiple levels,in particular in muscle-related phenotypes,highland and lowland populations show shallow genomic divergence and the colonization event occurred within the past few thousand years.In a one-month acclimation experiment investigating phenotypic plasticity,we exposed adult lowland tree sparrows to a hypoxic environment and did not observe muscle changes.Through population genetic analyses,we identified a signature of polygenic adaptation,whereby shifts in allele frequencies are spread across multiple loci,many of which are associated with muscle-related processes.Our results reveal a case of positive selection in which polygenic adaptation appears to drive rapid phenotypic evolution,shedding light on early stages of adaptive evolution to a novel environment. | Yanhua Qu Chunhai Chen Ying Xiong Huishang She Yong E Zhang Yalin Cheng Shane DuBay Dongming Li Per G P Ericson Yan Hao Hongyuan Wang Hongfeng Zhao Gang Song Hailin Zhang Ting Yang Chi Zhang Liping Liang Tianyu Wu Jinyang Zhao Qiang Gao Weiwei Zhai Fumin Lei | 2020 | National Science Review2020，7，1： | 2 |
| 15 | Effects of Slow-release Fertilizers and Sugarcane-specific Fertilizers on Yield and Quality of Sugarcane显示文摘The effects of slow-release fertilizers and sugarcane-specific fertilizers on the growth and quality of sugarcane were studied to provide a theoretical basis for cultivation of sugarcane with lower costs but higher efficiency. Field experiments were carried out in two major sugarcane areas in Guangxi and three fertilization treatments were studied: single application of compound fertilizers( treatment I),compound fertilizers + slow-release fertilizers( treatment II) and sugarcane-specific base fertilizers + sugarcane-specific topdressing( treatment III). Effects of equal fertilization conditions of treatment I,II and III on growth,yield and sugar of sugarcane were studied. The three fertilization treatments had little effects on emergence,tillering,and effective stems of sugarcane,but compared with the treatment of compound fertilizer( treatment I) with a ratio of N,P,and K of 1∶1∶1,treatment II using slow-release fertilizers as topdressing had better growth,higher plant height and stem diameter,so the yield was higher. Treatment III designed sugarcane-specific fertilizers with proper ratio according to fertilizer demands of sugarcane. Besides,the treatment III sugarcane-specific base fertilizers,containing certain amount of organic matters,could promote the sugar accumulation of sugarcane. Therefore,the sugarcane yield of treatment III was higher than that of treatment II. In conclusion,slow-release fertilizers and sugarcane-specific fertilizers can significantly increase sugarcane yield,especially sugarcane-specific fertilizers. Sugarcane-specific fertilizers have reasonable ratio and contain certain amount of organic matters,and can increase sugar content,obtain significant economic benefits,so it is worth popularization in large areas. | Qing LIAO Zepu JIANG Ying XING Shao'e YANG Guifen CHEN Panxia LIANG | 2018 | Asian Agricultural Research2018，10，6： | 2 |
| 16 | Evolution of interaction domain microstructure during spray deposition显示文摘 | LIANG X LAVEMIA E J | 1994 | Metall Mater Trans A1994，25，11： | 1 |
| 17 | Pervaporation of ethanol-water mixtures through polydimethylsiloxane-polystyrene interpenetrating polymer network supported membranes 显示文摘 | Liang L Ruckenstein E | 1996 | J Membr Sci1996，114，2： | 1 |
| 18 | Environmental effects on parasitic disease transmission exemplified by schistosomiasis in western China显示文摘 | Liang S Seto E Y Remais J V | 2007 | Proc Natl Acad Sci USA2007，104，17： | 1 |
| 19 | Profiling the humoral immune response to infection by using protcome microarrays: high-throughput vaccine and diagnostic antigen discovery显示文摘 | DAVIES D H LIANG X HERNANDEZ J E | 2005 | Proceedings of the National Academy of Sciences of the United States of America2005，102，3： | 1 |
| 20 | BODY PRESSURE DISTRIBUTION OF AUTOMOBILE DRIVING HUMAN MACHINE CONTACT INTERFACE显示文摘在汽车开车并且基于生理、解剖的原则瞄准疲劳和人机器的接触接口的舒适问题,在疲劳的形成和开发的肌肉和神经的生理、生物化学的进程系统地被分析。引起疲劳的生理的典型索引被印射到象肌肉压力紧张,血容器的压缩变形和神经 etc.from 一样的 biomechanicalindexes 形成机制的观点。骨骼和实质的几何模型被使用扫描 CT 的身体数据和 MRI 图象建立。舒适身体压力分发的一般规则通过屁股和大腿骨的区域的解剖结构的分析被获得。为容纳可调节的接触连接的 3D 的舒适的全面测试平台被构造。主观评估的人机器的接触接口和它的比较的身体压力分发的测试显示汽车驱使人机器的机械索引联系的简历连接,学习的身体压力分发统治有效地装评估人机器的接触的疲劳和舒适问题为最佳的设计连接并且提供理论基础人机器接触接口。 | CHEN Juan HONG Jun ZHANG E LIANG Jian LU Bingheng | 2007 | Chinese Journal of Mechanical Engineering2007，20，4： | 1 |
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