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| 1 | A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants显示文摘Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable.Here,we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation.We have also developed a rapid,cost-effective,and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting(HRM)analysis.Using tetraploid tobacco as a model species and the phytoene desaturase(PDS)gene as a target,we successfully created and expediently identified mutant plants,which were verified as tetra-allelic mutants.We produced pds mutant shoots at a rate of 47.5%from tobacco leaf explants,without the use of antibiotic selection.Among these pds plants,17.2%were confirmed to be non-transgenic,for an overall non-transgenic mutation rate of 8.2%.Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction.This method should be applicable to many economically important,heterozygous,perennial crop species that are more difficult to regenerate. | Longzheng Chen Wei Li Lorenzo Katin-Grazzini Jing Ding Xianbin Gu Yanjun Li Tingting Gu Ren Wang Xinchun Lin Ziniu Deng Richard J.McAvoy Frederick G.Gmitter Jr. Zhanao Deng Yunde Zhao Yi Li | 2018 | Horticulture Research2018，5，1： | 12 |
| 2 | Co-infections of SARS-CoV-2 with multiple common respiratory pathogens in infected patients显示文摘Dear Editor,In late December 2019,an outbreak of severe pneumonia caused by an unknown etiological agent was initially reported in Wuhan,China,and then quickly spread throughout China and even abroad(Zhu et al.,2020).Swift control measures and etiological investigations were conducted by the Chinese government,physicians and researchers,and by using high throughput sequencing and virological testing,the mysterious pneumonia pathogen was shown to be a novel coronavirus(SARS-CoV-2)capable of person-to-person transmission(Zhu et al.,2020).Coronaviruses are a group of non-segmented,enveloped and positive-sense RNA viruses that belong to the subfamily of Coronavirinae in the family of Coronavirdiae and order of Nidovirales. | Dachuan Lin Lei Liu Mingxia Zhang Yunlong Hu Qianting Yang Jiubiao Guo Yongchao Guo Youchao Dai Yuzhong Xu Yi Cai Xinchun Chen Zheng Zhang Kaisong Huang | 2020 | Science China(Life Sciences)2020，63，4： | 6 |
| 3 | The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii显示文摘Bamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value.Unique among traditional flowering plants,many bamboo plants undergo extensive synchronized flowering followed by large-scale death,seriously affecting the productivity and application of bamboo forests.To date,the molecular mechanism of bamboo flowering characteristics has remained unknown.In this study,a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1(SOC1)-like gene,BoMADS50,was identified from Bambusa oldhamii.BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B.oldhamii flowering and overexpression of BoMADS50 caused early fl owering in transgenic rice.Moreover,BoMADS50 could interact with APETALA1/FRUITFULL(AP1/FUL)-like proteins(BoMADS 14-1/2,BoMADS15-1/2)in vivo,and the expression of BoMADS50 was signi ficantly promoted by BoMADS14-1,further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B.oldhamii flowering.We also identi fi ed four additional transcripts of BoMADS50(BoMADS50-1/2/3/4)with different nucleotide variations.Although the protein-CDS were polymorphic,they had flowering activation functions similar to those of BoMADS50.Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE(SVP)-like gene BoSVP,but only BoMADS50-1 can positively induce their transcription.Therefore,nucleotide variations likely endow BoMADS50-1 with strong regulatory activity.Thus,BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B.oldhamii.Moreover,the functional conservatism and speci ficity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B.oldhamii. | Dan Hou Ling Li Tengfei Ma Jialong Pei Zhongyu Zhao Mengzhu Lu Aimin Wu Xinchun Lin | 2021 | Horticulture Research2021，8，1： | 4 |
| 4 | Genetic diversity analysis of Sinojackia microcarpa, a rare tree species endemic in China, based on simple sequence repeat markers显示文摘Although most Sinojackia species are endangered, they contribute greatly to the biodiversity of local ecosystems. Sinojackia microcarpa, an endangered species, is distributed only in three provinces of eastern China. Determining the genetic diversity of S. microcarpa provides key information for germplasm evaluation and species conservation. Here we used simple sequence repeat (SSR) markers to investigate the genetic diversity of eight natural populations of S. microcarpa. Leaf samples were collected from 144 individuals in 8 wild populations. The 156 bands were generated from 14 pairs of informative SSR primers, with an average percentage of polymorphic bands of 45.67%. The average values of Nei’s genetic diversity (He) and Shannon’s diversity index (I) were 0.1007 and 0.1658, respectively. The total genetic variation of S. microcarpa existed mainly within the eight populations, rather than among populations, and reached 86.41%. A cluster analysis showed that the eight wild populations of S. microcarpa could be classified into four groups, at a threshold of 4.0, based on an analysis of the SSR genotypes. Furthermore, there was a significant association between the phylogenetic relationships and the geographic locations of the S. microcarpa populations. In particular, populations from Fuyang, Jiande, and Lin’an in Zhejiang Province had close phylogenetic relationships and geographic distances. In addition, these three populations had the highest genetic diversity and the most individuals, suggesting that these three locations may be the S.microcarpa distribution center. This study serves as a model for studying the genetic diversity of endangered plant species. | Tailin Zhong Guangwu Zhao Yongfeng Lou Xinchun Lin Xiaomin Guo | 2019 | Journal of Forestry Research2019，30，3： | 2 |
| 5 | Understanding bamboo flowering based on large-scale analysis of expressed sequence tags 显示文摘 | LIN Xinchun HUANG Lichun FANG Wei | 2010 | Gen Mol Res2010，9，2： | 1 |
| 6 | A novel solid state fault current limiter for DC power distribution network显示文摘 | LUO Fang CHEN Jian LIN Xinchun | 2008 | IEEE Trans on Power Electronics2008，9，5： | 1 |
| 7 | Regulation of Macrophage Migration Inhibitory Factor Expression by Glucocorticoids in Vivo显示文摘 | Gunter Fingerle-Rowson Peter Koch Rachel Bikoff Xinchun Lin Christine N. Metz Firdaus S. Dhabhar Andreas Meinhardt Richard Bucala | 2003 | The American Journal of Pathology2003，，1： | 1 |
| 8 | An improved low-voltage ride-through control strategy of doubly fed induction generator during grid faults显示文摘 | Hu Sheng Lin Xinchun Kang Yong | 2011 | IEEE Transactions on Power Electronics2011，26，12： | 1 |
| 9 | Regula-tion of polymorphonuclear leukocyte apoptosis:role of lung en-dothelium-epithelium bilayer transmigration显示文摘 | MAOWEN HU XINCHUN LIN QIAOTING DU | 2005 | Am J PhysiolLung Cell Mol Physiol2005，288，2： | 1 |
| 10 | Analysis and design of the DSP-based fully digital - controlled UPS 显示文摘 | Pei Xuejun Lin Xinchun | 2010 | IEEE Power Electron -ics and Drive Systems2010，10，1： | 1 |
| 11 | Genetic similarity among cuhivars of Phyllostachys pubescens 显示文摘 | LIN Xinchun RUAN Xiaosai LOU Yongfeng | 2009 | Plant Syst Evol2009，277，： | 1 |
| 12 | IL‐33 synergizes with TCR and IL‐12 signaling to promote the effector function of CD8+ T cells显示文摘 | Qianting Yang Gang Li Yibei Zhu Lin Liu Elizabeth Chen Hēth Turnquist Xueguang Zhang Olivera J. Finn Xinchun Chen Binfeng Lu | 2011 | Eur. J. Immunol2011，，： | 1 |
| 13 | An improved low-voltage ride-through controlstrategy of doubly fed induction generator during grid faults显示文摘 | Hu Sheng Lin Xinchun Kang Yong | 2011 | IEEE Transactions on Power Electronics2011，26，12： | 1 |
| 14 | Overexpression of PvSVP1,an SVP-like gene of bamboo,causes early flowering and abnormal floral organs in Arabidopsis and rice显示文摘Bamboo is a nontimber woody plant featuring a long vegetative stage and uncertain flowering time.Therefore,the genes belonging to flowering repressors might be essential in regulating the transition from the vegetative to reproductive stage in bamboo.The Short Vegetative Phase(SVP)gene plays a pivotal role in floral transition and development.However,little is known about the bamboo SVP homologues.In this study,Phyllostachys violascens PvSVP1 is isolated by analysis of the P.edulis transcriptome database.Phylogenetic analysis shows that PvSVP1 is closely related to OsMADS55(rice SVP homolog).PvSVP1 is ubiquitously expressed in various tissues,predominantly in vegetative tissues.To investigate the function of PvSVP1,PvSVP1 is overexpressed in Arabidopsis and rice under the influence of the 35S promoter.Overexpression of PvSVP1 in Arabidopsis causes early flowering and produces abnormal petals and sepals.Quantitative real-time PCR reveals that overexpression in Arabidopsis produces an early flowering phenotype by downregulating FLC and upregulating FT and produces abnormal floral organs by upregulating AP1,AP3 and PI expressions.Simultaneously,overexpression of PvSVP1 in rice alters the expressions of flowering-related genes such as Hd3a,RFT1,OsMADS56 and Ghd7 and promotes flowering under field conditions.In addition,PvSVP1 may be a nuclear protein which interacts with PvVRN1 and PvMADS56 on the yeast two-hybrid and BiFC systems.Our study suggests that PvSVP1 may play a vital role in flowering time and development by interacting with PvVRN1 and PvMADS56 in the nucleus.Furthermore,this study paves the way toward understanding the complex flowering process of bamboo. | Shinan Liu Dan Hou Naresh Vasupalli Xinchun Lin | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
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