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| 1 | A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var.beijingensis显示文摘The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi.It is one of the important effective components in Agricultural Antibiotic120,which has been widely used as naturally-originated agents for treatment of crop decay in China.The chemical synthesis of anisomycin has recently been reported,but the complex process with low productivity made the biosynthesis still to be a vital mainstay in efforts.The biosynthetic gene cluster(BGC)of anisomycin in Streptomyces hygrospinosus var.beijingensis has been identified in our previous work,while poor understanding of the regulatory mechanism limited the yield enhancement via regulation engineering of S.hygrospinosus var.beijingensis.In this study here,we characterized AniF as an indispensable LuxR family transcriptional regulator for the activation of anisomycin biosynthesis.The genetic manipulations of aniF and the real-time quantitative PCR(RT-qPCR)revealed that it positively regulated the transcription of the anisomycin BGC.Moreover,the overexpression of aniF contributed to the improvement of the production of anisomycin and its derivatives.Dissection of the mechanism underlying the function of AniF revealed that it directly activated the transcription of the genes aniR-G involved in anisomycin biosynthesis.Especially,one AniF-binding site in the promoter region of aniR was identified by DNase I footprinting assay and an inverted repeat sequence(5′-GGGC-3′)composed of two 4-nt half sites in the protected region was found.Taken together,our systematic study confirmed the positive regulatory role of AniF and might facilitate the future construction of engineering strains with high productivity of anisomycin and its derivatives. | Jufang Shen Lingxin Kong Yan Li Xiaoqing Zheng Qing Wang Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 10 |
| 2 | 晚期非小细胞肺癌免疫检查点抑制剂新型生物标志物:血液PD-L1动态检测显示文摘背景免疫检查点抑制剂(immune checkpoint inhibitors,ICIs)具有良好疗效已成为恶性肿瘤治疗领域备受瞩目的翘楚。然而,仅有小部分患者获得长期临床获益。如何选择生物标志物来筛查ICIs治疗优势人群仍是一大热点。方法收集51例晚期恶性肿瘤患者的肿瘤组织和配对血样本,进行PD-L1表达的相关性分析。40例晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受ICIs治疗,2个月时检测血液PD-L1(bloodPD-L1,bPD-L1)表达的动态变化,包括PD-L1 mRNA、外泌体PD-L1(exosomalPD-L1,exoPD-L1)蛋白和可溶性PD-L1(solublePD-L1,sPD-L1)。应用R软件计算最佳截断值,用于预测最佳客观缓解(best objective response,b OR)、无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)。结果51例不同恶性肿瘤患者中,组织PD-L1(tissuePD-L1,tPD-L1)表达阳性患者的PD-L1mRNA表达水平显著升高。40例接受ICIs治疗的晚期NSCLC患者中,bPD-L1 mRNA上调≥2.04的患者b OR、PFS和OS更佳;21例接受ICIs治疗的晚期NSCLC患者中,exoPD-L1上调≥1.86的患者临床获益更大。sPD-L1动态变化与ICIs疗效无显著相关性。PD-L1 mRNA和exoPD-L1联合检测更有利于预测ICIs治疗疗效。结论bPD-L1表达与tPD-L1表达存在正相关;晚期NSCLC患者ICIs治疗初期PD-L1 mRNA和(或)exoPD-L1表达升高,可作为评估临床获益的新型生物标志物。 | Qiao YANG Mingjing CHEN Jiaoyang GU Kai NIU Xianlan ZHAO Linpeng ZHENG Zihan XU Yongxin YU Feng LI Lingxin MENG Zhengtang CHEN Wenlei ZHUO Luping ZHANG Jianguo SUN 杨峤(译) 孙建国(译) | 2021 | 中国肺癌杂志2021，24，12： | 10 |
| 3 | Quantitative investigation on collapse margin of steel high-rise buildings subjected to extremely severe earthquakes显示文摘Reponses of structures subjected to severe earthquakes sometimes significantly surpass what was considered in the design.It is important to investigate the failure mechanism and collapse margin of structures beyond design,especially for high-rise buildings.In this study,steel high-rise buildings using either square concrete-filled-tube(CFT) columns or steel tube columns are designed.A detailed three-dimensional(3 D) structural model is developed to analyze the seismic behavior of a steel high-rise towards a complete collapse.The effectiveness is verified by both component tests and a full-scale shaking table test.The collapse margin,which is defined as the ratio of PGA between the collapse level to the design major earthquake level(Level 2),is quantified by a series of numerical simulations using incremental dynamic analyses(IDA).The baseline building using CFT columns collapsed with a weak first story mechanism and presented a collapse margin ranging from 10 to 20.The significant variation in the collapse margin was caused by the different characteristics of the input ground motions.The building using equivalent steel columns collapsed earlier due to the significant shortening of the locally buckled columns,exhibiting only 57% of the collapse margin of the baseline building.The influence of reducing the height of the first story was quite significant.The shortened first story not only enlarged the collapse margin by 20%,but also changed the collapse mode. | Lin Xuchuan Mikiko Kato Zhang Lingxin Masayoshi Nakashima | 2018 | Earthquake Engineering and Engineering Vibration2018，17，3： | 9 |
| 4 | Orthogonal design to sift the optimal parameter of Neiguan acupuncture for cerebral infarction显示文摘The individual difference and non-repeatability in acupuncture have not only restricted the development of acupuncture,but have also affected the specificity of acupoints.The present study used instruments to control needle depth,lifting and thrusting frequency,and the duration of acupuncture.Effects of the quantified acupuncture were observed at Neiguan(PC6)with different stimulation parameters.A frequency of 1,2,or 3 Hz and duration of 5,60,or 180 seconds were used to observe cerebral blood flow and ratio of infarct volume recovery.Results showed that stimulation at Neiguan with a frequency of 1 Hz and long duration of 180 seconds or 2/3 Hz and long duration of 5/60seconds significantly increased cerebral blood flow and decreased the ratio of infarct volume.Interactions between frequency and duration play a critical role in quantified acupuncture therapy. | Yanan Zhang Sha Yang Xiaonong Fan Shu Wang Nina He Lingxin Li Ding Luo Xuemin Shi | 2013 | Neural Regeneration Research2013，8，28： | 7 |
| 5 | Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var.Hangzhouwanensis显示文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity. | Yan Li Lingxin Kong Jufang Shen Qing Wang Qian Liu Weinan Yang Zixin Deng Delin You | 2019 | Synthetic and Systems Biotechnology2019，4，1： | 4 |
| 6 | Theory,controls parameter and application of the packed-bed electroosmotic pump显示文摘Theory and controls parameters affecting the output pressure and flow rate of the packed-bed electroos-motic pump (p-EOP), including zeta potential z, electric in-tensity E, length of the column L, cross-section area of the column A, dielectric constant e and viscosity coefficient h of the liquid being pumped, are discussed concisely. And also, the fabrication and application of the p-EOP are introduced. | CHEN Lingxin, GUAN Yafeng, MA Jiping, SHU Xin & TAN Feng Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China Correspondence should be addressed to Guan Yafeng (e-mail: kfguan@ mail.dlptt.ln.cn) | 2003 | Chinese Science Bulletin2003，48，23： | 3 |
| 7 | Theoretical model of particle orientation distribution function in a cylindrical particle suspension subject to turbulent shear flow显示文摘为在平面狂暴的流动推迟的苗条圆柱的粒子的取向概率功能的方程被调查。在整体 averaging' 以后,为吝啬、波动的概率 functin 的方程被导出。为出现在吝啬的概率功能的方程的波动的概率功能的方程被导出。为出现在吝啬的概率功能的方程的波动的概率功能的方程被使用特征 analyisis 的 rthe 方法解决。orientational 分散在概率功能与吝啬的概率功能和 Larangian 液体速度关联有关的平均数的方程由于圆柱的粒子的随机的运动称为。吝啬的概率功能的进化被分散术语说明骚乱的使随机化的效果的修改 orientation-space-convectin equation' 描述。 | ZHANG Lingxin LIN Jianzhong ZHANG Weifeng | 2006 | Progress in Natural Science:Materials International2006，16，1： | 3 |
| 8 | A review of the research and application of deep learning-based computer vision in structural damage detection显示文摘Damage detection is a key procedure in maintenance throughout structures′life cycles and post-disaster loss assessment.Due to the complex types of structural damages and the low efficiency and safety of manual detection,detecting damages with high efficiency and accuracy is the most popular research direction in civil engineering.Computer vision(CV)technology and deep learning(DL)algorithms are considered as promising tools to address the aforementioned challenges.The paper aims to systematically summarized the research and applications of DL-based CV technology in the field of damage detection in recent years.The basic concepts of DL-based CV technology are introduced first.The implementation steps of creating a damage detection dataset and some typical datasets are reviewed.CV-based structural damage detection algorithms are divided into three categories,namely,image classification-based(IC-based)algorithms,object detection-based(OD-based)algorithms,and semantic segmentation-based(SS-based)algorithms.Finally,the problems to be solved and future research directions are discussed.The foundation for promoting the deep integration of DL-based CV technology in structural damage detection and structural seismic damage identification has been laid. | Zhang Lingxin Shen Junkai Zhu Baijie | 2022 | Earthquake Engineering and Engineering Vibration2022，21，1： | 2 |
| 9 | Method for solving dynamic equilibrium equations based on displacement excitation显示文摘Mode superposition is a widely used method for solving the dynamic equilibrium equation in structural dynamic analysis.However,the accuracy of this method may be reduced when the dynamic equilibrium equations are set up using displacement excitation.A new method for developing solutions for dynamic equilibrium equations based on displacement excitation is introduced.The dynamic equilibrium equation is decomposed into two parts,namely displacement excitation and velocity excitation,and precise integration and mode superposition methods are combined to solve the equation.Ritz vectors are then used to calculate the static response of the truncated modes of the structure,and a method for determining the number of participating modes is obtained.Using multi-degree-of-freedom systems as two computational examples,the differences in the structural responses obtained from the displacement excitation and acceleration excitation are compared and analyzed.It is shown that the new solution method generates consistent accuracy between the displacement excitation and acceleration excitation. | Xu Guolin Zhang Lingxin Bai Yashuang Sun Hao | 2020 | Earthquake Engineering and Engineering Vibration2020，19，2： | 2 |
| 10 | In Situ Electrochemical Mn(Ⅲ)/Mn(Ⅳ) Generation of Mn(Ⅱ)O Electrocatalysts for High-Performance Oxygen Reduction显示文摘Among various earth-abundant and noble metal-free catalysts for oxygen reduction reaction(ORR),manganese-based oxides are promising candidates owing to the rich variety of manganese valence.Herein,an extremely facile method for the synthesis of cubic and orthorhombic phase coexisting Mn(Ⅱ)O electrocatalyst as an efficient ORR catalyst was explored.The obtained MnO electrocatalyst with oxygen vacancies shows a significantly elevated ORR catalytic activity with a half-wave potential(E1/2) of as high as 0.895 V,in comparison with that of commercial Pt/C(E1/2=0.877 V).More impressively,the MnO electrocatalyst exhibits a marked activity enhancement after test under a constant applied potential for 1000 s thanks to the in situ generation and stable presence of high-valence manganese species(Mn^3+ and Mn^4+) during the electrochemical process,initiating a synergetic catalytic effect with oxygen vacancies,which is proved to largely accelerate the adsorption and reduction of O_2 molecules favoring the ORR activity elevation.Such an excellent ORR catalytic performance of this MnO electrocatalyst is applied in Zn-air battery,which shows an extra-high peak power density of 63.2 mW cm^-2 in comparison with that(47.4 m W cm^-2) of commercial Pt/C under identical test conditions. | Han Tian Liming Zeng Yifan Huang Zhonghua Ma Ge Meng Lingxin Peng Chang Chen Xiangzhi Cui Jianlin Shi | 2020 | Nano-Micro Letters2020，12，11： | 2 |
| 11 | Multimarker Detection of MAGE-1,MAGE-3 and AFP mRNAs by a Real-time Quantitative PCR Assay:a Possible Predictor of Hematogenous Micrometastasis of Hepatocellular Carcinoma显示文摘OBJECTIVE To explore the relationship between multimarker detection of MAGE-1,MAGE-3 and AFP mRNAs in the peripheral blood of patients with hepatocellular carcinoma and micrometastasis using a realtime quantitative-PCR(real-time Q-PCR)assay. METHODS Peripheral blood samples were obtained from control subjects and 86 patients with hepatocellular carcinoma (HCC).Real-time Q-PCR was used to detect MAGE-1,MAGE-3, and AFP mRNAs in the blood cells. RESULTS In 86 tumor specimens,the positivity for MAGE-1, MAGE-3,and AFP genes was respectively 34.9%(30/86),60.5% (52/86)and 69.8%(60/86).All specimens expressed at least one marker.MAGE-1,MAGE-3,and AFP transcripts were detected respectively in 12(14.0%),18(20.1%)and 29(33.7%)of the 86 blood specimens from hepatocellular carcinoma patients,while 45 specimens(52.3%)were positive for at least one marker.In addition,MAGE-1,MAGE-3 and AFP gene transcripts were not detected in any peripheral blood specimens from 25 chronic liver disease patients and 28 normal healthy volunteers.The positive rate correlated with the TNM clinical stages,extrahepatic metastasis and portal vein carcinothrombosis(P<0.05).No correlation was found between tumor size,tumor number, differentiation,serum a-fetoprotein(AFP)and the positive rate. CONCLUSION Our results indicate that a multimarker real- time Q-PCR assay with cancer-specific markers such as MAGE-1 and MAGE-3 in combination with a hepatocyte-specific AFP marker may be a promising diagnostic tool for monitoring hepatocellular carcinoma patients with better sensitivity and specificity. | Yuqing Zhang Qiang Li Ning Liu Tianqiang Song Rende Guo Lingxin Meng Xishan Hao | 2008 | Chinese Journal of Clinical Oncology2008，5，2： | 2 |
| 12 | Metabolic engineering of a methyltransferase for production of drug precursors demecycline and demeclocycline in Streptomyces aureofaciens显示文摘Demecycline(DMTC)and demeclocycline(DMCTC)are C6-demethylated derivatives of tetracycline(TC)and chlortetracycline(CTC),respectively.They are precursors of minocycline and tigecycline,which showed remarkable bioactivity against TC-resistant bacteria and have been used clinically for decades.In order to biosynthesize drug precursors DMTC and DMCTC,the function of a possible C-methyltransferase encoding gene ctcK was studied systematically in the CTC high-yielding industrial strain Streptomyces aureofaciens F3.TheΔctcK mutant accumulated two new products,which were turned out to be DMTC and DMCTC.Meanwhile,timecourse analysis of the fermentation products detected the epimers of DMTC and DMCTC transformed spontaneously.Finally,an engineering strain with higher productivity of DMCTC was constructed by deleting ctcK and overexpressing ctcP of three extra copies simultaneously.Construction of these two engineering strains not only served as a successful example of synthesizing required products through metabolic engineering,but also provided original strains for following elaborate engineering to synthesize more effective tetracycline derivatives. | Weinan Yang Lingxin Kong Qing Wang Zixin Deng Delin You | 2020 | Synthetic and Systems Biotechnology2020，5，3： | 2 |
| 13 | Dual synergetic catalytic effects boost hydrogen electric oxidation performance of Pd/W_(18)O_(49)显示文摘Developing anode catalysts of substantially enhanced activity for hydrogen oxidation reaction(HOR)and anti-CO poisoning performance is of great importance for the application of proton exchange membrane fuel cells(PEMFCs).Herein,we report Pd cluster in situ decorated urchin-like W_(18)O_(49)(WO_(2.72))electrocatalysts by a photo-reduction method for high performance HOR.The synthesized Pd-WO_(2.72)-L composite of low loading amount of 0.44 wt.%Pd by Xenon light reduction exhibits markedly high HOR catalytic activity and stability in 0.5 M H_(2)SO_(4),and the specific HOR current density and mass activity of Pd-WO_(2.72)-L are~1.5 and~80 times those of 20 wt.%Pt/C catalyst,respectively.Moreover,excellent anti-CO poisoning ability has also been obtained.The excellent HOR activity and anti-CO poisoning performance of Pd-WO_(2.72)-L have been discussed mainly in terms of the dual synergetic catalytic effects between Pd and WO_(2.72):Pd activation to Pd^(δ+)by the electron transfer from Pd to W promotes the hydrogen adsorption and activation to H*species,which results in largely elevated HOR activity;Pd degradation due to the CO poisoning is effectively prevented by WO_(2.72),which is responsible for the excellent CO-tolerance performance. | Lingxin Peng Han Tian Xiangzhi Cui Liang Su Ge Meng Zhonghua Ma Shaowen Cao Jianlin Shi | 2021 | Nano Research2021，14，7： | 2 |
| 14 | Contemporary medicinal chemistry strategies for the discovery and optimization of influenza inhibitors targeting vRNP constituent proteins显示文摘Influenza is an acute respiratory infectious disease caused by the influenza virus,affecting people globally and causing significant social and economic losses.Due to the inevitable limitations of vaccines and approved drugs,there is an urgent need to discover new anti-influenza drugs with different mechanisms.The viral ribonucleoprotein complex(vRNP)plays an essential role in the life cycle of influenza viruses,representing an attractive target for drug design.In recent years,the functional area of constituent proteins in vRNP are widely used as targets for drug discovery,especially the PA endonuclease active site,the RNA-binding site of PB1,the cap-binding site of PB2 and the nuclear export signal of NP protein.Encouragingly,the PA inhibitor baloxavir has been marketed in Japan and the United States,and several drug candidates have also entered clinical trials,such as favipiravir.This article reviews the compositions and functions of the influenza virus vRNP and the research progress on vRNP inhibitors,and discusses the representative drug discovery and optimization strategies pursued. | Lingxin Hou Ying Zhang Han Ju Srinivasulu Cherukupalli Ruifang Jia Jian Zhang Bing Huang Arianna Loregian Xinyong Liu Peng Zhan | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 2 |
| 15 | IL-17R activation of human periodontal ligament fibroblasts induces IL-23 p19 production:differential involvement of NF-B versus JNK/AP-I pathways 显示文摘 | Lingxin Zhu Yan Wu Hongxia Wei | 2011 | Mol Immunol2011，48，4： | 1 |
| 16 | Up-regulation of IL-23 p19 expression in human periodontal ligament fibrob- lasts by IL-lbeta via concurrent activation of the NF-kappaB and MAPKs/AP-1 pathways 显示文摘 | Lingxin Zhu Yan Wu Hongxia Wei | 2012 | Cytokine2012，60，1： | 1 |
| 17 | Occurrence of parabens in foodstuffs from China and its implications for human dietary exposure显示文摘 | CHUNYANG L LINGXIN C KURUNTHACHALAM K | 2013 | Environment International2013，5758，： | 1 |
| 18 | Licorice isoliquiritigenin suppresses RANKL-induced osteoclastogenesis in vitro and prevents inflammatory bone loss in vivo显示文摘 | Lingxin Zhu Hongxia Wei Yan Wu Shasha Yang Lan Xiao Jie Zhang Bin Peng | 2012 | International Journal of Biochemistry and Cell Biology2012，，： | 1 |
| 19 | Private Support and Public Assistance for Immigrant Families 显示文摘 | Lingxin Hao | 2003 | Journal of Marriage and Family2003，，1： | 1 |
| 20 | A study for performance of corn straw smashing device显示文摘 | Zhang Lijuan Geng Lingxin Shi Qingxiang | 2012 | Applied Mechanics and Materials2012，184，： | 1 |
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