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| 1 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 2 | A review of recent researches on Bunsen reaction for hydrogen production via S–I water and H2S splitting cycles显示文摘The Bunsen reaction is the center reaction for both the sulfur–iodine water splitting cycle for hydrogen production and the novel hydrogen sulfide splitting cycle for hydrogen and sulfuric acid production from the sulfur-containing gases.This paper reviews the research progress of the Bunsen reaction in recent 10–15 years.Researches were initially focused on the optimization of the operating conditions of the conventional Bunsen reaction requiring excessive water and iodine to improve the products separation efficiency and to avoid the side reactions and iodine vapor deposition.Alternative methods including electrochemical methods,precipitation methods,and non-aqueous solvent methods had their respective advantages,but still faced challenges.In development of the technology of H2S splitting cycle,dissolving iodine in toluene solvent could render the Bunsen reaction to occur with the flowable I2 stream at ambient temperature such that the side reactions and iodine vaporization can be avoided and the corrosion hazard lessened.It also prevented the Bunsen reaction from using excessive iodine and water.The products from the Bunsen reaction including HI,H2SO4,H2O,and toluene could be directly electrolyzed. | Ke Zhang Weiren Bao Liping Chang Hui Wangg | 2019 | Journal of Energy Chemistry2019，28，6： | 7 |
| 3 | Epstein-Barr virus-encoded RNAs as a survival predictor in nasopharyngeal carcinoma显示文摘 | Ke Kengjian Wang Haiyun Fu Sha Zhang Zichen Duan Liping Liu Dabo Ye Jin | 2014 | Chinese Medical Journal2014，，2： | 5 |
| 4 | LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration显示文摘The adult mammalian heart is thought to be a terminally differentiated organ given the postmitotic nature of cardiomyocytes.Consequently,the potential for cardiac repair through cardiomyocyte proliferation is extremely limited.Low-density lipoprotein receptor-related protein 6(LRP6)is a Wnt co-receptor that is required for embryonic heart development.In this study we investigated the role of LRP6 in heart repair through regulation of cardiomyocyte proliferation.Lrp6 deficiency increased cardiomyocyte cell cycle activity in neonatal,juvenile and adult mice.Cardiomyocyte-specific deletion of Lrp6 in the mouse heart induced a robust regenerative response after myocardial infarction(MI),led to reduced MI area and improvement in left ventricular systolic function.In vivo genetic lineage tracing revealed that the newly formed cardiomyocytes in Lrp6-deficient mouse hearts after MI were mainly derived from resident cardiomyocytes.Furthermore,we found that the pro-proliferative effect of Lrp6 deficiency was mediated by the ING5/P21 signaling pathway.Gene therapy using the adeno-associated virus(AAV)9 miRNAi-Lrp6 construct promoted the repair of heart injury in mice.Lrp6 deficiency also induced the proliferation of human induced pluripotent stem cell-derived cardiomyocytes(iPSC-CMs).Our study identifies LRP6 as a critical regulator of cardiomyocyte proliferation,which may lead to the development of a novel molecular strategy to promote myocardial regeneration and repair. | Yahan Wu Liping Zhou Hongyu Liu Ran Duan Huixing Zhou Fulei Zhang Xiaoyu He Dongbo Lu Ke Xiong Maolin Xiong Jinzhu Zhuang Yi Liu Li Li Dandan Liang Yi-Han Chen | 2021 | Cell Research2021，31，4： | 5 |
| 5 | Effect of glycine addition on the in-vitro corrosion behavior of AZ31 magnesium alloy in Hank’s solution显示文摘This study reports on the effect of the addition of Glycine to Hank’s solution on the in-vitro corrosion behavior of AZ31 magnesium(Mg)alloy at 37℃and a pH of 7.4 studied by using potentiodynamic polarization(PDP),hydrogen collecting techniques and electrochemical impedance spectroscopy(EIS)in combination with surface characterization techniques such as optical microscopy(OM),scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),X-ray diffraction(XRD)and X-ray photoelectron spectroscopy analysis(XPS).The results reveal that adsorption of glycine initially subdues the dissolution of AZ31 Mg alloy while in long run it enhances the dissolution of the alloy due to the commencement of the chelation effect of glycine with Ca^(2+) released from hydroxyapatite.The chelation of glycine with Ca^(2+) induces the formation of cracks in the surface film which further promotes the dissolution of AZ31 Mg alloy thereby forming a porous corrosion products layer on the surface of the alloy.As a result,both the continuous dissolution of AZ31 magnesium alloy and the hydrogen evolution rate(HER)are enhanced with increasing the immersion time in Hank’s solution. | Durga Bhakta Pokharel Liping Wu Junhua Dong Amar Prasad Yadav Dhruba Babu Subedi Madhusudan Dhakal Lin Zha Xin Mu Aniefiok Joseph Umoh Wei Ke | 2021 | Journal of Materials Science & Technology2021，，22： | 4 |
| 6 | Electrochemical noise analysis on the pit corrosion susceptibility of biodegradable AZ31 magnesium alloy in four types of simulated body solutions显示文摘Magnesium alloys have been investigated as biodegradable implant materials since the last century. Nonuniform degradation caused by local corrosion limits their application, and no appropriate technology has been used in the research. In this study, electrochemical noise has been used to study the pit corrosion on magnesium alloy AZ31 in four types of simulated body solutions, and the data have been analyzed using wavelet analysis and stochastic theory. Combining these with the conventional polarization curves, mass loss tests and scanning electron microscopy, the electrochemical noise results implied that AZ31 alloy in normal saline has the fastest corrosion rate, a high pit initiation rate, and maximum pit growth probability.In Hanks' balanced salt solution and phosphate-buffered saline, AZ31 alloy has a high pit initiation rate and larger pit growth probability, while in simulated body fluid, AZ31 alloy has the slowest corrosion rate, lowest pit initiation rate and smallest pit growth probability. | changgang wang liping wu fang xue rongyao ma ini-ibehe nabuk etim xuehui hao junhua dong wei ke | 2018 | Journal of Materials Science & Technology2018，34，10： | 4 |
| 7 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 8 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 9 | Regulation of Leaf Longevity by DML3-Mediated DNA Demethylation显示文摘Leaf senescence is driven by the expression of senescence-associated genes(SAGs).Development-specific genes often undergo DNA demethylation in their promoter and other regions,which regulates gene expression.Whether and how DNA demethylation regulates the expression of SAGs and thus leaf senescence remain elusive.Whole-genome bisulfite sequencing(WGBS)analyses of wild-type(WT)and demeter-like 3(dml3)Arabidopsis leaves at three developmental stages revealed hypermethylation during leaf senescence in dml3 compared with WT,and 20556 differentially methylated regions(DMRs)were identified by comparing the methylomes of dml3 and WT in the CG,CHG,and CHH contexts.Furthermore,we identified that 335 DMR-associated genes(DMGs),such as NAC016 and SEN1,are upregulated during leaf senescence,and found an inverse correlation between the DNA methylation levels(especially in the promoter regions)and the transcript abundances of the related SAGs in WT.In contrast,in dml3 the promoters of SAGs were hypermethylated and their transcript levels were remarkably reduced,and leaf senescence was significantly delayed.Collectively,our study unraveled a novel epigenetic regulatory mechanism underlying leaf senescence in which DML3 is expressed at the onset of and during senescence to demethylate promoter,gene body or 3'UTR regions to activate a set of SAGs. | Lu Yuan Dan Wang Liwen Cao Ningning Yu Ke Liu Yongfeng Guo Susheng Gan Liping Chen | 2020 | Molecular Plant2020，13，8： | 3 |
| 10 | Effect of applied potential on the microstructure, composition and corrosion resistance evolution of fluoride conversion film on AZ31 magnesium alloy显示文摘AZ31 magnesium(Mg) alloy was potentiostatic polarized in 0.1 M deaerated KF solution with pH 7.5 from-0.4 V to-1.4 V with an interval of-0.2 V. The polarization process was described by the potentiostatic current decay. The resultant film was analyzed by scanning electron microscopy(SEM), X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS) and electrochemical impedance spectroscopy(EIS). The results demonstrated that the deposited film included a Mg(OH)_2/MgF_2 containing inner layer and a Mg(OH)-2/MgF_2/KMgF_3 comprising outer layer. The high polarized potential produced high content of MgF_2 but low content of KMgF_3 and thin film. Conversely, the low polarized potential produced small content of MgF_2 but high content of KMgF_3 and thick film. The optimal corrosion resistance of the deposited film was obtained at-1.4 V, which was closely related with the content of MgF_2 and KMgF_3 and the film thickness. | Liping Wu Changgang Wang Durga Bhakta Pokharel Ini-Ibehe Nabuk Etim Lin Zhao Junhua Dong Wei Ke Nan Chen | 2018 | Journal of Materials Science & Technology2018，34，11： | 3 |
| 11 | Symmetry-enforced three-dimensional Dirac phononic crystals显示文摘Dirac semimetals,the materials featuring fourfold degenerate Dirac points,are critical states of topologically distinct phases.Such gapless topological states have been accomplished by a band-inversion mechanism,in which the Dirac points can be annihilated pairwise by perturbations without changing the symmetry of the system.Here,we report an experimental observation of Dirac points that are enforced completely by the crystal symmetry using a nonsymmorphic three-dimensional phononic crystal.Intriguingly,our Dirac phononic crystal hosts four spiral topological surface states,in which the surface states of opposite helicities intersect gaplessly along certain momentum lines,as confirmed by additional surface measurements.The novel Dirac system may release new opportunities for studying elusive(pseudo)and offer a unique prototype platform for acoustic applications. | Xiangxi Cai Liping Ye Chunyin Qiu Meng Xiao Rui Yu Manzhu Ke Zhengyou Liu | 2020 | Light(Science & Applications)2020，9，1： | 2 |
| 12 | New insights into the microstructure of the friction surface layer of C/C composites显示文摘 | Baoling Lei Lianlong He Maozhong Yi Liping Ran Huijuan Xu Yicheng Ge Ke Peng | 2011 | Carbon2011，，13： | 2 |
| 13 | Multi-objective optimal allocation strategy for the energy internet in Huangpu District, Guangzhou, China显示文摘To improve the overall efficiency of the energy system,the basic structure for the energy internet of coordination and optimization of“generation-grid-load-storage”of Huangpu District,Guangzhou,China is designed,while the arrangement for the output of centralized and distributed energy module and energy storage are proposed.Taking economic benefit maximization,environmental benefit maximization and energy efficiency maximization as sub-objectives,the mathematical model of multi-objective optimal allocation and operation strategy of the energy internet is established considering supply-demand balance constraints,equipment characteristic constraints,operation mode constraints,and energy conditions constraints.The calculation results show that without considering the outsourced electricity,the balanced strategy,the economic development strategy,the environmental protection strategy,and the energy efficiency strategy are obtained by calculation,which are all superior to the traditional energy supply strategy.Moreover,considering the outsourced electricity,the proportion of outsourced electricity to total electricity is 19.8%,which is the system optimization of the energy internet under certain power demand.Compared with other strategies without outsourced electricity,the outsourced electricity strategy can have a certain emission reduction effect,but at the same time reduce the economic benefit.Furthermore,the huge difference in demand for thermal and cooling load between industrial and commercial areas results in the installed capacity of gas distributed energy stations in industrial areas being nearly twice as large as that in commercial areas.The distributed photovoltaic power generation is allocated according to the proportion of the installed roof areas of photovoltaic power generation system in residential,industrial,and commercial areas. | Pei LI Guotian CAI Yuntao ZHANG Shangjun KE Peng WANG Liping GAO | 2020 | Frontiers in Energy2020，14，2： | 2 |
| 14 | Medium Access Control: A Physical Layer Network- Coded Approach显示文摘 | ZHANG Yu XIONG Ke DU Liping | 2014 | China Communications2014，11，A02： | 2 |
| 15 | In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy显示文摘 | Liping Xu Feng Pan Guoning Yu Lei Yang Erlin Zhang Ke Yang | 2008 | Biomaterials2008，，8： | 2 |
| 16 | In?vitro corrosion behaviour of Mg alloys in a phosphate buffered solution for bone implant application显示文摘 | Liping Xu Erlin Zhang Dongsong Yin Songyan Zeng Ke Yang | 2008 | Journal of Materials Science: Materials in Medicine2008，，3： | 1 |
| 17 | Microstructure, mechanical and corrosion properties and biocompatibility of Mg–Zn–Mn alloys for biomedical application显示文摘 | Erlin Zhang Dongsong Yin Liping Xu Lei Yang Ke Yang | 2008 | Materials Science & Engineering C2008，，： | 1 |
| 18 | In?vitro corrosion behaviour of Mg alloys in a phosphate buffered solution for bone implant application显示文摘 | Liping Xu Erlin Zhang Dongsong Yin Songyan Zeng Ke Yang | 2008 | Journal of Materials Science: Materials in Medicine2008，，3： | 1 |
| 19 | 显示文摘 | Zhang Erlin Xu Liping Yang Ke | 2005 | Scripta Materials2005，535，： | 1 |
| 20 | Effect of D-fructose on the in-vitro corrosion behavior of AZ31 magnesium alloy in simulated body fluid显示文摘The effect of addingd-fructose to simulated body fluid(SBF) on the corrosion behavior of AZ31 magnesium(Mg) alloy at 37.C and at a pH of 7.4 was studied by potentiodynamic polarization(PDP), electrochemical impedance spectroscopy(EIS), potentiostatic polarization and hydrogen(H2) collecting techniques,Raman spectroscopy technique, scanning electron microscopy(SEM), energy dispersive spectroscopy(EDS), X-ray diffraction(XRD), X-ray photoelectron spectroscopy analysis(XPS) and Fourier transformed infrared(FTIR). The results demonstrated that the addition of fructose enhanced the deposition of phosphates forming thick and compact corrosion products, which inhibited the transmission of aggressive ions into the Mg substrate. As a result, both the anodic dissolution of Mg and negative difference effect(NDE) were suppressed. Thus, the corrosion resistance of AZ31 Mgalloy in SBF was significantly improved. | Durga Bhakta Pokharel Liping Wu Junhua Dong Xin Wei Ini-Ibehe Nabuk Etim Dhruba Babu Subedi Aniefiok Joseph Umoh Wei Ke | 2021 | Journal of Materials Science & Technology2021，，7： | 1 |
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