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| 1 | Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus显示文摘What is already known on this topic?A novel human coronavirus,known as SARS-CoV-2 or 2019-nCoV,is the causative agent of the coronavirus disease 2019(COVID-19).We have released the primers and probes of real-time reverse transcription polymerase chain reaction(rRT-PCR)assays for the laboratory detection of COVID-19 infection.What is added by this report?Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab,N,and E genes for detection of COVID-19 infection.The application of rRT-PCR assays among four types of specimens(alveolar lavage,sputum,throat swabs,and stool)from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens.What are the implications for public health practice?These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity,specificity,and applicability.Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19. | Peihua Niu Roujian Lu Li Zhao Huijuan Wang Baoying Huang Fei Ye Wenling Wang Wenjie Tan | 2020 | China CDC weekly2020，2，25： | 8 |
| 2 | Two-tube multiplex real-time reverse transcription PCR to detect six human coronaviruses显示文摘Dear Editor,Coronaviruses are enveloped positive-strand RNA viruses with 27–33 kb genomes.These viruses are classified into four genera,namely Alphacoronavirus,Betacoronavirus,Gammacoronavirus,and | Peihua Niu Jun Shen Na Zhu Roujian Lu Wenjie Tan | 2016 | Virologica Sinica2016，31，1： | 4 |
| 3 | 2022 Chinese expert consensus and guidelines on clinical management of toxicity in anti-CD19 chimeric antigen receptor T-cell therapy for B-cell non-Hodgkin lymphoma显示文摘Adoptive cellular immunotherapy with chimeric antigen receptor(CAR)T cells has emerged as a novel modality for treating relapsed and/or refractory B-cell non-Hodgkin lymphoma(B-NHL).With increasing approval of CAR T-cell products and advances in CAR T cell therapy,CAR T cells are expected to be used in a growing number of cases.However,CAR T-cell-associated toxicities can be severe or even fatal,thus compromising the survival benefit from this therapy.Standardizing and studying the clinical management of these toxicities are imperative.In contrast to other hematological malignancies,such as acute lymphoblastic leukemia and multiple myeloma,anti-CD19 CAR T-cell-associated toxicities in B-NHL have several distinctive features,most notably local cytokine-release syndrome(CRS).However,previously published guidelines have provided few specific recommendations for the grading and management of toxicities associated with CAR T-cell treatment for B-NHL.Consequently,we developed this consensus for the prevention,recognition,and management of these toxicities,on the basis of published literature regarding the management of anti-CD19 CAR T-cell-associated toxicities and the clinical experience of multiple Chinese institutions.This consensus refines a grading system and classification of CRS in B-NHL and corresponding measures for CRS management,and delineates comprehensive principles and exploratory recommendations for managing anti-CD19 CAR T-cell-associated toxicities in addition to CRS. | Ping Li Yang Liu Yun Liang Jian Bo Sujun Gao Yongxian Hu Yu Hu He Huang Xiaojun Huang Hongmei Jing Xiaoyan Ke Jianyong Li Yuhua Li Qifa Liu Peihua Lu Heng Mei Ting Niu Yongping Song Yuqin Song Liping Su Sanfang Tu Jianxiang Wang Depei Wu Zhao Wang Kailin Xu Zhitao Ying Qingming Yang Yajing Zhang Fengxia Shi Bin Zhang Huilai Zhang Xi Zhang Mingfeng Zhao Weili Zhao Xiangyu Zhao Liang Huang Jun Zhu Wenbin Qian Weidong Han Aibin Liang | 2023 | Cancer Biology & Medicine2023，20，2： | 3 |
| 4 | A core-shell structured COYID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity显示文摘Although inoculation of COVID-19 vaccines has rolled out globally,there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries.Here,we report on the development of a highly efficacious mRNA vaccine,SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex(LPP)nanoparticles.SWOT 23 is easy to produce using a large-scale microfluidics-based apparatus.The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability,and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration.Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123,represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2,but also a panel of variants including D614G and N501Y variants.In addition,SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge.Taken together,SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans. | Ren Yang Yao Deng Baoying Huang Lei Huang Ang Lin Yuhua Li Wenling Wang Jingjing Liu Shuaiyao Lu Zhenzhen Zhan Yufei Wang Ruhan A Wen Wang Peihua Niu Li Zhao Shiqiang Li Xiaopin Ma Luyao Zhang Yujian Zhang Weiguo Yao Xingjie Liang Jincun Zhao Zhongmin Liu Xiaozhong Peng Hangwen Li Wenjie Tan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
| 5 | Development of design directed engineering curriculum based on CDIO Framework 显示文摘 | Peihua Gu Xiaohua Lu Guangjin Xiong Shengping Li and Minfen Shen | 2006 | World Transaction on Engineering and Technology Education2006，5，2： | 1 |
| 6 | Development of design directed en- gineering cun'iculum based on CDIO Framework 显示文摘 | Peihua Gu Xiaohua Lu Guangjin Xiong Shengping Li Minfen Shen | 2006 | World Transaction on Engineering and Technology E- ducation2006，5，2： | 1 |
| 7 | A new approach for robust design of mechanical systems显示文摘 | GU Peihua LU B SPIEWAK S | 2004 | Annals of the CIRP2004，53，1： | 1 |
| 8 | A limited memory algorithm forIxmnd constrained optimization显示文摘 | Byrd R H Lu Peihua Nocedal J | 1995 | SIAM Journal on Scientific Com-puting1995，16，5： | 1 |
| 9 | Genome Characterization of COVID-19 Lineage B.1.1.7 Detected in the First Six Patients of a Cluster Outbreak——Shenzhen City,Guangdong Province,China,May 2021显示文摘Screening for coronavirus disease 2019(COVID-19)virus,also known as SARS-CoV-2,infection every seven days was performed for high-risk populations who worked at the Yantian Port in Yantian District,Shenzhen City,Guangdong Province.On May 20,2021,an oropharyngeal swab from a 44-year-old male(Case A)tested preliminarily positive for COVID-19 by a quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)method in a third-party laboratory. | Yaqing He Peihua Niu Bo Peng Ying Sun Ziquan Lyu Renli Zhang Xiang Zhao Yang Song Yenan Feng Xiujuan Tang Dongfeng Kong Xinyi Wei Can Zhu Qingju Lu Shimin Li Xiaoliang Xiao Weiwen Liu Junjia He Junjie Xia Long Chen Xuan Zou Tiejian Feng | 2021 | China CDC weekly2021，3，25： | 0 |
| 10 | Profiles of SARS-CoV-2 RNA and Antibodies in Inpatients with COVID-19 not Related with Clinical Manifestation: A Single Centre Study显示文摘Dear Editor,A novel coronavirus disease(COVID-19)caused by acute respiratory syndrome coronavirus 2(SARS-Co V-2)first broke out in Wuhan,Hubei Province,China,in 2019.More than 50 million cases of COVID-19 have been confirmed globally with 1,280,868 deaths reported as of November12,2020(http://gffzze7c39cdc9e894ca6sx5b9bxx0unxn6qf5.ffgz.tsg.suse.edu.cn/). | Li Zhao Ruqin Gao Roujian Lu Huijuan Wang Yao Deng Peihua Niu Fachun Jiang Baoying Huang Jiwei Liang Jing Jia Feng Zhang Wenling Wang Guizhen Wu Wenjie Tan | 2021 | Virologica Sinica2021，36，5： | 0 |
| 11 | Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method显示文摘What is already known on this topic?Coronavirus disease 2019(COVID-19),a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)or COVID-19 virus,was reported in December 2019.Complete genomes of the COVID-19 virus from clinical samples using next generation sequencing(NGS)have been reported.What is added by this report?Here we provide the technical data for sequencing complete genome of COVID-19 virus from clinical samples using the Sanger method.Two complete COVID-19 virus genome sequences(named WH19004-S and GX0002)were obtained from clinical samples of COVID-19 patients,and two single nucleotide polymorphisms(SNPs)in ORF7a(T/C,nt 27,493)and ORF8(T/C,nt 28,253)of WH19004-S were identified by Sanger sequencing.What are the implications for public health practice?The COVID-19 virus genome sequencing by Sanger method reported here could be used to generate data of high enough quality without requirement for expensive NGS equipment,which support sequencing complete genomes from clinical samples and monitoring of viral genetic variations of COVID-19 infections. | Roujian Lu Peihua Niu Li Zhao Huijuan Wang Wenling Wang Wenjie Tan | 2020 | China CDC weekly2020，2，25： | 0 |
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