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| 1 | Full time-domain nonlinear coupled dynamic analysis of a truss spar and its mooring/riser system in irregular wave显示文摘A new full time-domain nonlinear coupled method has been established and then applied to predict the responses of a Truss Spar in irregular wave.For the coupled analysis,a second-order time-domain approach is developed to calculate the wave forces,and a finite element model based on rod theory is established in three dimensions in a global coordinate system.In numerical implementation,the higher-order boundary element method(HOBEM)is employed to solve the velocity potential,and the 4th-order Adams-Bashforth-Moultn scheme is used to update the second-order wave surface.In deriving convergent solutions,the hull displacements and mooring tensions are kept consistent at the fairlead and the motion equations of platform and mooring-lines/risers are solved simultaneously using Newmark-integration scheme including Newton-Raphson iteration.Both the coupled quasi-static analysis and the coupled dynamic analysis are performed.The numerical simulation results are also compared with the model test results,and they coincide very well as a whole.The slow-drift responses can be clearly observed in the time histories of displacements and mooring tensions.Some important characteristics of the coupled responses are concluded. | YANG MinDong TENG Bin XIAO LongFei NING DeZhi SHI ZhongMin QU Yan | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，1： | 8 |
| 2 | Expression of Chlamydomonas actin-gfp fusion gene in tobacco suspension cell and polymerization of the actin-gfp protein in vitro显示文摘The fusion gene of actin (cDNA of Chlamydo-monas reinhardtii) and green fluorescence protein (gfp) had been constructed into two expression vectors which could be expressed in E. coli and tobacco suspension cells BY2. The correct expression was observed in E. coli and BY2 with a fluorescence microscopy. The fusion protein, which took part in the membrane skeleton, was mainly located peripherally along the membrane, specially the fusion protein was distributed around nucleus and cell plate, while the fusion protein also forms F-actin in the cell. The fusion protein was purified from B121plus by ammonium sulfate fractionation, ion exchange chromatography and hydrophobic interaction chromatography. The purified production could polymerize into F-actin when the actin polymerizing buffer was added.It was demonstrated that the characteristics and function of actin in Chlamydomonas was similar with those of animals and higher plants. | YANG Yi LIU Guoqin YAN Longfei | 2001 | Chinese Science Bulletin2001，46，10： | 4 |
| 3 | Searching for the Nano-Hertz Stochastic Gravitational Wave Background with the Chinese Pulsar Timing Array Data ReleaseⅠ显示文摘Observing and timing a group of millisecond pulsars with high rotational stability enables the direct detection of gravitational waves(GWs).The GW signals can be identified from the spatial correlations encoded in the times-of-arrival of widely spaced pulsar-pairs.The Chinese Pulsar Timing Array(CPTA)is a collaboration aiming at the direct GW detection with observations carried out using Chinese radio telescopes.This short article serves as a“table of contents”for a forthcoming series of papers related to the CPTA Data Release 1(CPTA DR1)which uses observations from the Five-hundred-meter Aperture Spherical radio Telescope.Here,after summarizing the time span and accuracy of CPTA DR1,we report the key results of our statistical inference finding a correlated signal with amplitude logA_(c)=-14.4_(-2.8)^(+1.0)for spectral index in the range ofα∈[-1.8,1.5]assuming a GW background(GWB)induced quadrupolar correlation.The search for the Hellings–Downs(HD)correlation curve is also presented,where some evidence for the HD correlation has been found that a 4.6σstatistical significance is achieved using the discrete frequency method around the frequency of 14 n Hz.We expect that the future International Pulsar Timing Array data analysis and the next CPTA data release will be more sensitive to the n Hz GWB,which could verify the current results. | Heng Xu Siyuan Chen Yanjun Guo Jinchen Jiang Bojun Wang Jiangwei Xu Zihan Xue RNicolas Caballero Jianping Yuan Yonghua Xu Jingbo Wang Longfei Hao Jingtao Luo Kejia Lee Jinlin Han Peng Jiang Zhiqiang Shen Min Wang Na Wang Renxin Xu Xiangping Wu Richard Manchester Lei Qian Xin Guan Menglin Huang Chun Sun Yan Zhu | 2023 | Research in Astronomy and Astrophysics2023，23，7： | 3 |
| 4 | From pollen actin to crop male sterility显示文摘Actin plays an important role in the life activity of animal and plant cells. Pollen cells have plenty of actin whose structure and characteristics are very similar to the animal actin. The nucleotide sequence and amino acid sequence of plant actin gene are very similar to those of the animal gene. The content of pollen actin from male sterile plants is much more lower than that from its maintainer plants. The expression of actin gene is organ-specific during the plant development. The expression quantity of actin gene in pollen is much more higher than those from root, stem and leaf. The expression plasmid of the anti-sense actin gene was constructed, transferred to the protoplasts of wheat and tomato to inhibit the expression of actin gene in pollen and thus the male sterile plants of wheat and tomato were obtained. The actin in pollens from the transgenic plants was reduced significantly, whereas the pistil was not affected. This study might pave a new way to breeding male sterile lines for the | YAN Longfei LIU Guoqin XIAO Xingguo | 2000 | Chinese Science Bulletin2000，45，9： | 3 |
| 5 | Experimental Study on Dynamic Mechanical Properties of Sprayed Concrete-Surrounding Rock Combined Body显示文摘To investigate the dynamic response problem of the double medium formed by the adherence of sprayed concrete and surrounding rock in the tunnel,a split Hopkinson pressure bar of 75 mm in diameter was adopted at the ages of 3,7 and 10 d.Experimental results showed that dynamic compressive strength and dynamic increase factors(DIF)of the combined bodies increase with the strain rate.With the growth of strain rate,the critical strain of the combined bodies first increases,then deceases.Furthermore,the combined bodies of 3 d reveal the plastic property and brittle property for 7 d and 10 d when the strain rate is over 80/s.The failure characteristic of the sprayed concrete changes from tearing strain damage to crushing damage as the growth of strain rate,and the failure characteristic of rock presents the tensile failure mode as demonstrated by the scanning electron microscope(SEM). | Dongming Guo Pengyang Yan Longfei Fan Yingshi Zhang Xiaoye Wang | 2019 | Journal of Beijing Institute of Technology2019，28，2： | 3 |
| 6 | High-resolution structure-from-motion models covering 160 km-long surface ruptures of the 2021 M_(W)7.4 Madoi earthquake in northern Qinghai-Tibetan Plateau显示文摘The May 222021 M_(W)7.4 Madoi,Qinghai,China earthquake presented a rare opportunity to apply the modern unmanned aerial vehicle(UAV)photography method in extreme altitude and weather conditions to image surface ruptures and near-field effects of earthquake-related surface deformations in the remote Tibet.High-resolution aerial photographs were acquired in the days immediately following the mainshock.The complex surface rupture patterns associated with this event were covered comprehensively at 3-6 cm resolution.This effort represents the first time that an earthquake rupture in the interior of the Qinghai-Tibetan Plateau has been fully and systematically captured by such high-resolution imagery,with an unprecedented level of detail,over its entire length.The dataset has proven valuable in documenting subtle and transient rupture features,such as the significant mole-tracks and opening fissures,which were ubiquitous coseismically but degraded during the subsequent summer storm season.Such high-quality imagery also helps to document with high fidelity the fractures of the surface rupture zone(supplements of this paper),the pattern related to how the faults ruptured to the ground surface,and the distribution of off-fault damage.In combination with other ground-based mapping efforts,the data will be analyzed in the following months to better understand the mechanics of earthquake rupture related to the fault zone rheology,rupture dynamics,and frictional properties along with the fault interface. | Jing Liu-Zeng Wenqian Yao Xiaoli Liu Yanxiu Shao Wenxin Wang Longfei Han Yan Wang Xianyang Zeng Jinyang Li Zijun Wang Zhijun Liu Hongwei Tu | 2022 | Earthquake Research Advances2022，2，2： | 3 |
| 7 | Graph Computing Based Security Constrained Unit Commitment in Hydro-thermal Power Systems Incorporating Pumped Hydro Storage显示文摘This paper proposes a graph computing based mixed integer programming(MIP)framework for solving the security constrained unit commitment(SCUC)problem in hydro-thermal power systems incorporating pumped hydro storage(PHS).The proposed graph computing-based MIP framework considers the economic operations of thermal units,cascade hydropower stations and PHS stations,as well as their technical impacts towards the network security.First,the hydro-thermal power system data and unit information are stored in a graph structure with nodes and edges,which enables nodal and hierarchical parallel computing for the unit commitment(UC)solution calculation and network security analysis.A MIP model is then formulated to solve the SCUC problem with the mathematical models of thermal units,cascade hydropower stations and PHS stations.In addition,two optimization approaches including convex hull reformulation(CHR)and special ordered set(SOS)methods are introduced for speeding up the MIP calculation procedure.To ensure the system stability under the derived UC solution,a parallelized graph power flow(PGPF)algorithm is proposed for the hydro-thermal power system network security analysis.Finally,case studies of the IEEE 118-bus system and a practical 2749-bus hydro-thermal power system are introduced to demonstrate the feasibility and validity of the proposed graph computing-based MIP framework. | Longfei Wei Guangyi Liu Shen Yan Renchang Dai Yachen Tang | 2021 | CSEE Journal of Power and Energy Systems2021，7，3： | 2 |
| 8 | The Crosstalk of mTOR/S6K1 and Hedgehog Pathways显示文摘 | Yan Wang Qingqing Ding Chia-Jui Yen Weiya Xia Julie G. Izzo Jing-Yu Lang Chia-Wei Li Jennifer L. Hsu Stephanie A. Miller Xuemei Wang Dung-Fang Lee Jung-Mao Hsu Longfei Huo Adam M. LaBaff Dongping Liu Tzu-Hsuan Huang Chien-Chen Lai Fuu-Jen Tsai Wei-Chao Ch | 2012 | Cancer Cell2012，，3： | 2 |
| 9 | Membrane skeleton spectrin in pollen and pollen tube显示文摘Immuno-western blots, immunofluorescence and immuno-gold labeling methods were used to study the existence and distribution of a homologue of the membrane skeleton spectrin in the pollen and pollen tube of Lilium davidii Duch. A spectrin homologue was found to exist in the pollen and pollen tube, distributed on membranes of secretory vesicles near Golgi apparatus. | Yan Li Longfei Yan S. Y. Zee B. Q. Huang | 1999 | Chinese Science Bulletin1999，44，10： | 2 |
| 10 | Actin and myosin during pollen germination显示文摘Actin and myosin from pollen tubes of Lilium davidii were studied by using immunoblotting, Dot_Blot and myosin Ca 2+_ATPase analysis. On immunoblotting of the total soluble pollen tube proteins, anti_α_actin antibody labelled a polypeptide approximately 43 ku, which is considered to be the actin of lily. The mRNA encoding actin in ungerminated pollen and germinated pollen were both undetectable in our experiments. A myosin exhibited Ca 2+_ATPase activity, with a native molecular weight of 460 ku has been identified by using immunoblotting. A polypeptide of about 205 ku and a polypeptide of about 20 ku were the heavy chain and a set of light chain of the myosin, which can crossreact with anti_skeletal muscle myosin heavy chain monoclonal antibody and anti_skeletal muscle myosin light chain (20 ku) monoclonal antibody, respectively. The Ca 2+_ATPase activities of myosin in crude extracts of germinated pollen were positively related to the growth rates of pollen tubes. | Dongtao Ren Shengcheng Han Longfei Yan | 1998 | Chinese Science Bulletin1998，43，8： | 1 |
| 11 | Association between polymorphisms in the apolipoprotein D gene and sporadic Alzheimer's disease显示文摘 | Yan Chen Longfei Jia | 2008 | Brain Research2008，1233，： | 1 |
| 12 | Adiabatic shear banding instability in bulk metallic glasses 显示文摘 | Dai Lanhong Yan Min Liu Longfei | 2005 | Applied Physics Letters2005，87，14： | 1 |
| 13 | Environmental selection and evolutionary process jointly shape genomic and functional profiles of mangrove rhizosphere microbiomes显示文摘Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning.However,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycling of rhizosphere microbiomes remains unclear.To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres,we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies.Compared with the native mangrove(Kandelia obovata,KO),the introduced mangrove(Sonneratia apetala,SA)rhizosphere microbiome had significantly(p<0.05)higher average genome size(AGS)(5.8 vs.5.5 Mb),average 16S ribosomal RNA gene copy number(3.5 vs.3.1),relative abundances of mobile genetic elements,and functional diversity in terms of the Shannon index(7.88 vs.7.84)but lower functional potentials involved in CH4 cycling(e.g.,mcrABCDG and pmoABC),N2 fixation(nifHDK),and inorganic S cycling(dsrAB,dsrC,dsrMKJOP,soxB,sqr,and fccAB).Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere.Additionally,salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes.This study advances our understanding of microbially mediated biogeochemical cycling of CH_(4),N,and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions,which has important implications for future mangrove reforestation. | Xiaoli Yu Qichao Tu Jihua Liu Yisheng Peng Cheng Wang Fanshu Xiao Yingli Lian Xueqin Yang Ruiwen Hu Huang Yu Lu Qian Daoming Wu Ziying He Longfei Shu Qiang He Yun Tian Faming Wang Shanquan Wang Bo Wu Zhijian Huang Jianguo He Qingyun Yan Zhili He | 2023 | mLife2023，2，3： | 0 |
| 14 | Benefit analysis of multi-approach biomass energy utilization toward carbon neutrality显示文摘To reduce greenhouse gas(GHG)emissions,biomass has been increasingly developed as a renewable and clean alternative to fossil fuels because of its carbon-neutral characteristics.China has been investigating the rational development and use of bioenergy for developing its clean energy and achieving carbon neutrality.Substituting fossil fuels with multi-source and multi-approach utilized bioenergy and corresponding carbon reduction in China remain largely unexplored.Here,a comprehensive bioenergy accounting model with a multi-dimensional analysis was developed by combining spatial,life cycle,and multi-path analyses.Accordingly,the bioenergy production potential and GHG emission reduction for each distinct type of biomass feedstock through different conversion pathways were estimated.The sum of all available organic waste(21.55 EJ yr^(-1))and energy plants on marginal land(11.77 EJ yr^(-1))in China produced 23.30 EJ of bioenergy and reduced 2,535.32 Mt CO_(2)-eq emissions,accounting for 19.48%and 25.61%of China’s total energy production and carbon emissions in 2020,respectively.When focusing on the carbon emission mitigation potential of substituting bioenergy for conventional counterparts,bioelectricity was the most effective,and its potential was 4.45 and 8.58 times higher than that of gaseous and liquid fuel alternatives,respectively.In this study,life cycle emission reductions were maximized by a mix of bioenergy end uses based on biomass properties,with an optimal 78.56%bioenergy allocation from biodiesel,densified solid biofuel,biohydrogen,and biochar.The main regional bioenergy GHG mitigation focused on the Jiangsu,Sichuan,Guangxi,Henan,and Guangdong provinces,contributing to 31.32%of the total GHG mitigation potential.This study provides valuable guidance on exploiting untapped biomass resources in China to secure carbon neutrality by 2060. | Jiaoyue Wang Jingying Fu Zhitong Zhao Longfei Bing Fengming Xi Feng Wang Jiang Dong Shiyun Wang Gang Lin Yan Yin Qinqin Hu | 2023 | The Innovation2023，4，3： | 0 |
| 15 | Membrane bioreactors for hospital wastewater treatment: recent advancements in membranes and processes显示文摘Discharged hospital wastewater contains various pathogenic microorganisms,antibiotic groups,toxic organic compounds,radioactive elements,and ionic pollutants.These contaminants harm the environment and human health causing the spread of disease.Thus,effective treatment of hospital wastewater is an urgent task for sustainable development.Membranes,with controllable porous and nonporous structures,have been rapidly developed for molecular separations.In particular,membrane bioreactor(MBR)technology demonstrated high removal efficiency toward organic compounds and low waste sludge production.To further enhance the separation efficiency and achieve material recovery from hospital waste streams,novel concepts of MBRs and their applications are rapidly evolved through hybridizing novel membranes(non hydrophilic ultrafiltration/microfiltration)into the MBR units(hybrid MBRs)or the MBR as a pretreatment step and integrating other membrane processes as subsequent secondary purification step(integrated MBR-membrane systems).However,there is a lack of reviews on the latest advancement in MBR technologies for hospital wastewater treatment,and analysis on its major challenges and future trends.This review started with an overview of main pollutants in common hospital wastewater,followed by an understanding on the key performance indicators/criteria in MBR membranes(i.e.,solute selectivity)and processes(e.g.,fouling).Then,an in-depth analysis was provided into the recent development of hybrid MBR and integrated MBR-membrane system concepts,and applications correlated with wastewater sources,with a particular focus on hospital wastewaters.It is anticipated that this review will shed light on the knowledge gaps in the field,highlighting the potential contribution of hybrid MBRs and integrated MBRmembrane systems toward global epidemic prevention. | Yan Zhao Yangbo Qiu Natalie Mamrol Longfei Ren Xin Li Jiahui Shao Xing Yang Bart van der Bruggen | 2022 | Frontiers of Chemical Science and Engineering2022，16，5： | 0 |
| 16 | dBrms1 Acts as a Positive Regulator of Notch Signaling in Drosophila Wing显示文摘The highly conserved Notch signaling is precisely regulated at different steps in a series of developmental events. However, little is known about the regulation of Notch receptor at transcriptional level. Here, we demonstrate that dBrms1 is involved in regulating Notch signaling in Drosophila wing. We show that knockdown of dBrms1 by RNA interference(RNAi) in wing disc suppresses the expression of Notch signaling target genes wingless(wg), cut and Enhancer of split m8 [E(spl)m8]. Consistently, the levels of Wg and Cut are reduced in the dBrms1 mutant clones. Importantly, loss of dBrms1 leads to significant reduction of Notch proteins. Furthermore, depletion of dBrms1 results in apparent downregulation of Notch transcription in the wing disc. Moreover, we find that dBrms1 is functionally conserved with human Breast cancer metastasis suppressor 1 like(hBRMS1L) in the modulation of Notch signaling. Taken together, our data provide important insights into the biological function of dBrms1 in regulating Notch signaling. | Qinghai Zhang Yan Zhang Longfei Wu Yongfei Yang Xue Li Lei Gao Xiaomeng Hou Yihui Wu Guoli Hou Zhouhua Li Xinhua Lin | 2014 | Journal of Genetics and Genomics2014，41，6： | 0 |
| 17 | Review on microorganisms of glacial snow and ice on the Tibetan Plateau显示文摘Over the past 15 years there has been much effort invested in microorganisms of glacial snow and ice on the Tibetan Plateau.These studies include:phenotypic characteristics of recovered isolates;factors(dust,temperature,altitude)influencing microbial abundance,diversity and community in one glacier;distribution of bacterial number,diversity,community along ice core depth;similarities and differences of regionally distributed ice core isolates;seasonal variation of bacterial abundance and diversity.The following need further study.Better methods for more information about the diversity,survival mechanism of glacial microorganism;more research about archaea and fungi;microbial resources;relation of glacial microorganisms with biogeochemical cycle and mass balance;research on altitude distribution. | ZHANG Shuhong HOU Shugui QIN Xiang YAN Peiying ZHAO Longfei LIANG Feng | 2013 | 商丘师范学院学报2013，29，12： | 0 |
| 18 | Research progress of large size SiC single crystal materials and devices显示文摘SiC semiconductor is the focus of recent international research.It is also an important raw material for China to achieve carbon emission peak and carbon neutrality.After nearly 20 years of research and development,we focus on the three types SiC crystals,n-type,p-type and semi-insulating,indicating the development of Shandong University for crystal growth.And defects control,electrical property,atomic polishing,and corresponding device authentication all obtain great progress.Total dislocation density of 6-inch n-type substrates decreases to 2307 cm^(-2),where BPD(Basal Plane Dislocation)lowers to 333 cm^(-2) and TSD(Threading Screw Dislocation)19 cm^(-2).The full width at half maximum(FWHM)(0004)rocking curves is only 14.4 arcsec.The resistivity reaches more than 1E+12Ω·cm for semi-insulating SiC and lower than 20 mΩ·cm for n-type SiC.The impurity concentrations in 6-inch high-purity semi-insulating(HPSI)SiC crystals reach extreme low levels.The devices made of various substrate materials have good performance. | Xiufang Chen Xianglong Yang Xuejian Xie Yan Peng Longfei Xiao Chen Shao Huadong Li Xiaobo Hu Xiangang Xu | 2023 | Light(Science & Applications)2023，12，1： | 0 |
| 19 | Evaluation of different primers of the 18S rRNA gene to profile amoeba communities in environmental samples显示文摘Amoeboid protists,an assemblage of organisms belonging to different phylogenetic lineages,have drawn increasing attention due to their crucial ecological roles in various environments and their potential health risks.Currently,18S rRNA gene sequencing is widely applied for the detection of amoebae.However,it is not clear which is the best primer pair for 18S rRNA gene amplification in amoebae.This study compared the four most commonly used primer pairs for revealing the diversity,composition,core species,and community assembly processes of amoebae in water and sediments.We found that the choice of primers artificially influences the detection of community composition of amoebae.We also found that short-read fragments may lead to mismatches in taxonomy and were not suitable for phylogenetic analyses.In contrast,full-length primers could detect the highest number of amoeba lineages and annotate 80%of reads belonging to amoebae to known species.However,full-length primers did not detect as many amoeba species as V4 primers.Moreover,we showed that beta diversity and community assembly determination were largely unaffected by primer choice,but different primers could influence our interpretations of the ecological process underlying stochasticity and determinism.This study indicates that full-length read sequencing and V4 region Illumina sequencing are suitable for profiling amoeba diversity in the environment. | Xiafei Zheng Zhili He Cheng Wang Qingyun Yan Longfei Shu | 2022 | Water Biology and Security2022，1，3： | 0 |
| 20 | Effects of acupotomy on the activity of osteoclasts and osteoblasts in the subchondral bone of rabbits with early and mid-stage knee osteoarthritis models显示文摘Objective:To investigate whether acupotomy could inhibit subchondral bone remodeling in knee osteoarthritis(KOA)rabbits by regulating the activity of osteoblasts and osteoclasts.Methods:KOA rabbits were prepared by immobilization for 6 and 9 weeks by Videman method.Nine groups of rabbits(control,6 weeks and 9 weeks model,6 weeks and 9 weeks acupotomy,6 weeks and 9 weeks electroacupuncture,and 6 weeks and 9 weeks drug groups)received acupotomy,electroacupuncture and risedronate sodium intervention,respectively,for 3 weeks.Results:Acupotomy can inhibit the activity of osteoclasts and osteoblasts in subchondral bone by reducing the proteins expression of cathepsin K(CK)and tartrate-resistant acid phosphatase(TRAP)and decreasing the proteins expression of osteocalcin(OCN)and alkaline phosphatase(ALP),to intercept the abnormal bone resorption and bone formation of subchondral bone in 6-week and 9-week immobilization-induced KOA rabbits.Conclusion:These findings indicated that acupotomy may be more advantageous than risedronate sodium intervention in modulating subchondral bone remodeling in KOA rabbits,especially in 9-week immobilization-induced KOA rabbits. | Mira Lee Yan Guo Xilin Chen Longfei Xing Wei Zhang Lia Chang Changqing Guo | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，3： | 0 |
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