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| 1 | Promoter methylation of Wnt/β-Catenin signal inhibitor TMEM88 is associated with unfavorable prognosis of nonsmall cell lung cancer显示文摘Objective:Recent research has indicated that altered promoter methylation of oncogenes and tumor suppressor genes is an important mechanism in lung cancer development and progression.In this study,we investigated the association between promoter methylation of TMEM88,a possible inhibitor of the Wnt/β-Catenin signaling,and the survival of patients with nonsmall cell lung cancer(NSCLC).Methods:Twelve pairs of tumor and adjacent non-tumor samples were used for microarray analyses of DNA methylation and gene expression.For validation,more than two hundred additional samples were analyzed for methylation using bisulfite pyrosequencing and for gene expression using q RT-PCR.Then the cell function were tested by wound healing,transwell,CCK8 and cell cycle assay.Results:Our analysis of patient specimens showed that TMEM88 methylation was higher in NSCLC tumors(82.2%±10.3,P<0.01)compared with the adjacent normal tissues(65.9%±7.2).The survival analysis revealed that patients with high TMEM88methylation had a shorter overall survival(46 months)compared with patients with low TMEM88 methylation(>56 months;P=0.021).In addition,we found that demethylation treatment could inhibit tumor cell proliferation,migration,and invasion,which was supportive of an association between methylation and survival.Conclusions:Based on these consistent observations,we concluded that TMEM88 may play an important role in NSCLC progression and that promoter methylation of TMEM88 may serve as a biomarker for NSCLC prognosis and treatment. | Rongna Ma Nannan Feng Xiao Yu Hongyan Lin Xiaohong Zhang Oumin Shi Huan Zhang Shuo Zhang Lei Li Min Zheng Ming Gao Herbert Yu Biyun Qian | 2017 | Cancer Biology & Medicine2017，14，4： | 6 |
| 2 | Estimation of chlorophyll content in Brassica napus based on unmanned aerial vehicle images显示文摘The chlorophyll content has a direct effect on photosynthesis of crops.In order to explore a quick and convenient method for estimating the chlorophyll content of Brassica napus and facilitate efficient crop monitoring,we measured the actual value of chlorophyll with a SPAD-502 chlorophyll detector,and collected aerial images of B.napus with an unmanned aerial vehicle(UAV)carrying a RGB camera in this study.The total number of 270samples collected images were divided into regions according to the planting conditions of different B.napus varieties in the field.Then,according to the empirical formula,there were 36 colors’characteristic parameters calculated and combined.To estimate the chlorophyll content of rape,189 samples were included in the modeling set,while the other 81 samples were enrolled in the validation set for testing the accuracy of this model.After the combination of R(red),G(green)and B(blue)color channels,the results showed that the color characteristics B/(R+G),b,B/G,(G-B)/(G+B),g-b were highly connected with the measured value of chlorophyll SPAD,and the correlation coefficient between the combination based on B/(R+G)and SPAD value was 0.747.With R2=0.805,RMSE=3.343,and RE=6.84%,the regression model created using random forest had superior outcomes,according to the model comparison.This study offers a new method for quickly estimating the amount of chlorophyll in rapeseed and a workable reference for crop monitoring using the UAV platform. | Yayi Huang Qiming Ma Xiaoming Wu Hao Li Kun Xu Gaoxiang Ji Fang Qian Lixia Li Qian Huang Ying Long Xiaojun Zhang Biyun Chen Changhua Liu | 2022 | Oil Crop Science2022，7，3： | 1 |
| 3 | Nicotinamide mononucleotide improves spermatogenic function in streptozotocin-induced diabetic mice via modulating the glycolysis pathway显示文摘Spermatogenic dysfunction is one of the major secondary complications of diabetes;however,the underlying mechanisms remain ill-defined,and there is no available drug or strategy for the radical treatment of diabetic spermatogenic dysfunction.Therefore,the objective of this study is to investigate the protective effects of nicotinamide mononucleotide(NMN)on testicular spermatogenic function in streptozotocin(STZ)-induced diabetic mice.The results show that oral administration of NMN significantly increases the body and testis weight and the number of sperms.Moreover,the abnormal sperm count and the rate of sperm malformation are significantly decreased compared with the saline-treated diabetic mice.Histological analysis reveals that NMN treatment significantly increases the area and diameter of seminiferous tubules,accompanied by an increased number of spermatogenic cells and sperms.Immunohistochemistry and qRT-PCR results show that NMN increases Bcl-2 expression and decreases Bax expression in the testis.NMN also increases the protein expression of Vimentin and the mRNA expressions of WT1 and GATA4.In addition,qRT-PCR,western blot analysis and immunohistochemistry results also show that NMN increases the expressions of glycolysis-related rate-limiting enzymes including HK2,PKM2,and LDHA.In summary,this study demonstrates the protective effects of NMN on the testis in an STZ-induced diabetic mice model.NMN exerts its protective effects via reducing spermatogenic cell apoptosis by regulating glycolysis of Sertoli cells in diabetic mice.This study provides an experimental basis for the future clinical application of NMN in diabetes-induced spermatogenicdysfunction. | Duo Ma Linlin Hu Jinyuan Wang Min Luo Aihong Liang Xiaocan Lei Biyun Liao Meixiang Li Ming Xie Haicheng Li Yiwei Gong Dan Zi Xiangrun Li Xi Chen Xucai Liao | 2022 | Acta Biochimica et Biophysica Sinica2022，54，9： | 1 |
| 4 | Basic Analysis of Two-Coils Structure Based on Magnetically-Coupled Resonant Technology for SWIPT Application显示文摘The Magnetically-coupled resonant(MCR)technology is exploited in this paper to realize Synchronous wireless information and power transfer(SWIPT) function, which means that the power carriers also transmit information. The circuit structure of SWIPT system is analyzed and the existence of two optimal frequencies in power efficiency under small resistance circumstance is proved.The physical parameters having influences on the two optimal frequencies are discussed, such as the distance between coils, impedance characteristics of coils and loads.These results provide a way to increase the bandwidth of MCR technology, while maintaining high power efficiency to realize SWIPT function. Simulations and experimental results are presented to verify the feasibility of the proposed system and obtained theoretical expressions. | MA Biyun CHEN Boheng LI Sumei WANG Yide WEI Gang | 2015 | Chinese Journal of Electronics2015，24，4： | 1 |
| 5 | Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii显示文摘The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii. | Xue Liu Lingxia Zhou Biyun Luo Hongli Qian Baoqing Ye Keyi Ma Gaofeng Qiu | 2023 | Aquaculture and Fisheries2023，8，5： | 0 |
| 6 | CACA Guidelines for Holistic Integrative Management of Breast Cancer显示文摘Purpose:Breast cancer is now the most common malignant tumor worldwide.About one-fourth of female cancer patients all over the world sufer from breast cancer.And about one in six female cancer deaths worldwide is caused by breast cancer.In terms of absolute numbers of cases and deaths,China ranks frst in the world.The CACA Guidelines for Holistic Integrative Management of Breast Cancer were edited to help improve the diagnosis and comprehensive treatment in China.Methods:The Grading of Recommendations Assessment,Development and Evaluation(GRADE)was used to classify evidence and consensus.Results:The CACA Guidelines for Holistic Integrative Management of Breast Cancer include the epidemiology of breast cancer,breast cancer screening,breast cancer diagnosis,early breast cancer treatment,advanced breast cancer treatment,follow-up,rehabilitation,and traditional Chinese medicine treatment of breast cancer patients.Conclusion:We to standardize the diagnosis and treatment of breast cancer in China through the formulation of the CACA Guidelines. | Jiong Wu Daiming Fan Zhimin Shao Binghe Xu Guosheng Ren Zefei Jiang Yongsheng Wang Feng Jin Jin Zhang Qingyuan Zhang Fei Ma Jinli Ma Zhonghua Wang Shusen Wang Xiaojia Wang Shu Wang Haibo Wang Tao Wang Xiang Wang Jing Wang Jia Wang Biyun Wang Li Fu Hongyuan Li Yehui Shi Lu Gan Yunjiang Liu Jian Liu Zhenzhen Liu Qiang Liu Qiang Sun Wenwu Cheng Keda Yu Zhongsheng Tong Xinhong Wu Chuangui Song Jianguo Zhang Jian Zhang Junjie Li Bin Li Man Li Huiping Li Wentao Yang Hongjian Yang Benlong Yang Hong Bu Juping Shen Zhenzhou Shen Yiding Chen Ceshi Chen Da Pang Zhimin Fan Ying Zheng Xiaoli Yu Guangyu Liu Xichun Hu Yiqun Ling Jinhai Tang Yongmei Yin Cuizhi Geng Peng Yuan Yajia Gu Cai Chang Xuchen Cao Yuan Sheng Yuanxi Huang Jian Huang Weijun Peng Xiaohua Zeng Yuntao Xie Ning Liao Committee of Breast Cancer Society,Chinese Anti-Cancer Association | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
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