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18篇 您的检索式:作者名="MA Xingguo"
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1Improvement of wheat drought and salt tolerance by expression of a stress- inducible transcription factorGmDREB of soybean (Glycinemax)显示文摘Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants.GAO Shiqing XU Huijun CHENG Xianguo CHEN Ming XU Zhaoshi LI Liancheng YE Xingguo DU Lipu HAO Xiaoyan MA Youzhi 2005Chinese Science Bulletin2005,50,23:30
2Effects of Er on the microstructure and properties of AZ31 magnesium alloy prepared via the EMS process显示文摘The effects of small amounts of the rare-earth element erbium on the microstructure and the mechanical properties of AZ31 magnesium alloy via the electromagnetic stirring(EMS) process have been studied.It has been shown that AZ31-Er alloys are mainly composed of α-Mg solid solution and β-Mg17Al12 phases.When the Er content reaches 0.12 wt.%,the characteristic peaks of Al2Er can be observed.The micro-structure is obviously refined and the tensile strength of the AZ31-based alloy at ambient temperature is significantly improved by contents of 0.03 wt.% Er,especially the elongation(δ=19%).More addition of Er obviously decreases the tensile strength and elongation of the AZ31-based alloy because of the grain coarsening and the reduction of β-Mg17Al12 phases.MA Yutao,ZHANG Xingguo,LIU Hongbin,and MENG Linggang School of Materials Science and Engineering,Dalian University of Technology,Dalian 116023,China 2010Rare Metals2010,29,4:6
3Gut microbial profiles and the role in lipid metabolism in Shaziling pigs显示文摘Shaziling pig,a Chinese indigenous breed,has been classified as a fatty pig model.However,the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported.Here,we compared the lipid metabolic and microbial profiles at 30,60,90,150,210,and 300 d of age between Shaziling and Yorkshire pigs.Predictably,there were marked differences in the liver lipids(i.e.,cholesterol,glucose,and low-density lipoprotein)and the lipid related expressions(i.e.,SREBP1/2,LXRa/b,DGAT1/2,and FABP1-3)between Shaziling and Yorkshire pigs.Bacteria sequencing in the ileal digesta and mucosa showed that Shaziling pigs had a higher a-diversity and higher abundances of probiotics,such as Lactobacillus johnsonii,Lactobacillus amylovorus,and Clostridium butyricum.Thirty-five differentiated metabolites were further identified in the mucosa between Shaziling and Yorkshire pigs,which were enriched in the carbohydrate,protein,glucose and amino acid metabolism and bile acid biosynthesis.Furthermore,7 differentiated microbial species were markedly correlated with metabolites,indicating the role of gut microbiota in the host metabolism.Next,the role of differentiated L.johnsonii in lipid metabolism was validated in Duroc×Landrace×Yorkshire(DLY)pigs and the results showed that L.johnsonii mono-colonization promoted lipid deposition and metabolism by altering gut microbiota(i.e.,Megasphaera elsdenii and L.johnsonii)and DGAT1/DGAT2/CD36-PPARg gene expressions.In conclusion,Shaziling pigs exhibited different metabolic and microbial profiles compared with Yorkshire pigs,which might have contributed to the diverse metabolic phenotypes,and the significant enrichment of L.johnsonii in Shaziling pigs promoted lipid metabolism and obesity of DLY pigs,which provided a novel idea to improve the fat content of lean pigs.Jie Ma Yehui Duan Rui Li Xiaoxiao Liang Tiejun Li Xingguo Huang Yulong Yin Jie Yin 2022Animal Nutrition2022,,2:6
4Perspectives and challenges of hydrogen storage in solid-state hydrides显示文摘Hydrogen has been widely considered as a clean energy carrier that bridges the energy producers and energy consumers in an efficient and safe way for a sustainable society.Hydrogen can be stored in a gas,liquid and solid states and each method has its unique advantage.Though compressed hydrogen and liquefied hydrogen are mature technologies for industrial applications,appropriate measures are necessary to deal with the issues at high pressure up to around 100 MPa and low temperature at around 20 K.Distinct from those technologies,storing hydrogen in solid-state hydrides can realize a more compact and much safer approach that does not require high hydrogen pressure and cryogenic temperature.In this review,we will provide an overview of the majormaterial groups that are capable of absorbing and desorbing hydrogen reversibly.The main features on hydrogen storage properties of each material group are summarized,together with the discussion of the key issues and the guidance of materials design,aiming at providing insights for new material development as well as industrial applications.Zhen Chen Zhongliang Ma Jie Zheng Xingguo Li Etsuo Akiba Hai-Wen Li 2021Chinese Journal of Chemical Engineering2021,34,1:4
5Biomarkers of aging显示文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu 2023Science China(Life Sciences)2023,66,5:2
6Non-aqueous capillary electrophoresis for separation and simultaneous determination of fraxin,esculin and esculetin in Cortex fraxini and its medicinal preparations显示文摘Cunhong Li Anjia Chen Xiaofeng Chen Xiao Ma Xingguo Chen Zhide Hu 0,,:1
7Liquid meta-services:A component-based operating system layer for pervasive computing显示文摘Ma Bo Zhang Yi Shi Xingguo 2004Advances in Embedded Software and System2004,,11:1
8The Mechanism Design and Testing Study on Accelerator Based on The Internal Parallel Moving Gears显示文摘WANG Guosheng MA Xingguo WANG Liangdong 2012Applied Mechanics and Materials2012,,:1
9Iron-Catalyzed Aerobic Oxidation of Aldehydes: Single Component Catalyst and Mechanistic Studies显示文摘向在在空中把纯氧或氧的 1 atm 用作氧化剂和单个部件催化剂的催化数量的 MeCN 的羧基的酸的醛的氧气的氧化, Fe (没有 3)3Xingguo Jiang Yizhan Zhai Junyu Chen Yulin Han Zheng Yang Shengming Ma 2018Chinese Journal of Chemistry2018,36,1:1
10Effect of A12 O3 layer on improving high temperature oxidation resistance of siliconized TiAl-based alloy显示文摘Ma Xiaoxia Liang Wei Zhao Xingguo 2006Materials Letters2006,60,1314:1
11Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L显示文摘Maoyun She Guixiang Yin Jiarui Li Xing Li Lipu Du Wujun Ma Xingguo Ye 2013Molecular Biotechnology2013,,2:1
12Boosting the capability of Li_(2)C_(2)O_(4)as cathode pre-lithiation additive for lithium-ion batteries显示文摘Li_(2)C_(2)O_(4),with a high theoretical capacity of 525 mAh·g^(−1)and good air stability,is regarded as a more attractive cathode prelithiation additive in contrast to the reported typical inorganic pre-lithiation compounds which are quite air sensitive.However,its obtained capacity is much lower than the theoretical value and its delithiation potential(>4.7 V)is too high to match with the most commercial cathode materials,which greatly impedes its practical application.Herein,we greatly improve the pre-lithiation performance of Li_(2)C_(2)O_(4)as cathode additive with fulfilled capacity at a much-reduced delithiation voltage,enabling its wide applicability for typical commercial cathodes.We increase the capacity of Li_(2)C_(2)O_(4)from 436 to 525 mAh·g^(−1)by reducing its particle size.Through optimizing the types of conductive additives,introducing nano-morphological NiO,MnO2,etc.as catalysts,and innovatively designing a bilayer electrode,the delithiation potential of Li_(2)C_(2)O_(4)is successfully reduced from 4.778 to 4.288 V.We systematically study different particle size,conductive additives,and catalysts on the delithiation behavior of Li_(2)C_(2)O_(4).Finally,it is applied to pre-lithiate the hard carbon anode,and it is found that Li_(2)C_(2)O_(4)could effectively increase the capacity of the full cell from 79.0 to 140.0 mAh·g^(−1)in the first cycle.In conclusion,our study proves that improving the reactivity is an effective strategy to boost the pre-lithiation of Li_(2)C_(2)O_(4).Guxin Huang Jianing Liang Xingguo Zhong Haoyue Liang Can Cui Cheng Zeng Shuhao Wang Mengyi Liao Yue Shen Tianyou Zhai Ying Ma Huiqiao Li 2023Nano Research2023,16,3:0
13Arabidopsis pollen-specific glycerophosphodiester phosphodiesterase-like genes are essential for pollen tube tip growth显示文摘In angiosperms,pollen tube growth is critical for double fertilization and seed formation.Many of the factors involved in pollen tube tip growth are unknown.Here,we report the roles of pollenspecific GLYCEROPHOSPHODIESTER PHOSPHO DIESTERASE-LIKE(GDPD-LIKE)genes in pollen tube tip growth.Arabidopsis thaliana GDPD-LIKE6(At GDPDL6)and At GDPDL7 were specifically expressed in mature pollen grains and pollen tubes and green fluorescent protein(GFP)-At GDPDL6 and GFP-At GDPDL7 fusion proteins were enriched at the plasma membrane at the apex of forming pollen tubes.Atgdpdl6 Atgdpdl7 double mutants displayed severe sterility that was rescued by genetic complementation with At GDPDL6 or At GDPDL7.This sterility was associated with defective male gametophytic transmission.Atgdpdl6 Atgdpdl7 pollen tubes burst immediately after initiation of pollen germination in vitro and in vivo,consistent with the thin and fragile walls in their tips.Cellulose deposition was greatly reduced along the mutant pollen tube tip walls,and the localization of pollen-specific CELLULOSE SYNTHASE-LIKE D1(CSLD1)and CSLD4 was impaired to the apex of mutant pollen tubes.A rice pollen-specific GDPD-LIKE protein also contributed to pollen tube tip growth,suggesting that members of this family have conserved functions in angiosperms.Thus,pollen-specific GDPDLIKEs mediate pollen tube tip growth,possibly by modulating cellulose deposition in pollen tube walls.Chong Wang Hao Cheng Wenjing Xu Jingshi Xue Xinguo Hua Guimin Tong Xujun Ma Chuanping Yang Xingguo Lan Shi-Yi Shen Zhongnan Yang Jirong Huang Yuxiang Cheng 2023Journal of Integrative Plant Biology2023,65,8:0
14Microstructures and mechanical properties of AZ31-0.1Ca magnesium alloy produced by soft-contact electromagnetic casting and hot extrusion显示文摘An AZ31-0.1Ca magnesium alloy produced by Soft-contact electromagnetic continuous casting(SEMC) was investigated.The fine homogeneous structure and the precipitated phases were obtained by SEMC.The effects of microalloying of Ca and middle frequency electromagnetic field on AZ31-0.1Ca magnesium alloy were discussed.And the as-cast billets were extruded with different extrusion ratios subsequently.The alloy showed an ultrafine grain size of 2-5 μm due to dynamic recrytallization(DRX) in the course of hot extrusion.The comprehensive properties of the as-extruded alloy are the best at the extrusion ratio of 25,and the hardness,UTS and elongation are 73.9 HB,356.8 MPa and 16.5%,respectively.Zheng REN Xingguo ZHANG Hai HAO Li SUI Tao ZHANG Yutao MA 2010Acta Metallurgica Sinica(English Letters)2010,23,2:0
153-44 Monte Carlo Simulation Study on Optimizing the Active Beam Delivery System at HIRFL显示文摘To obtain carbon ion beam suitable for the active spot scanning beam delivery system at the Heavy Ion ResearchFacility in Lanzhou (HIRFL), the Monte Carlo (MC) program SHIELD-HIT12A was used to study the influencesof beam delivery distance and structure period of mini ridge filter on full width at the half maximum (FWHM) ofbeam spot and dose flatness at the iso-center of the treatment room.Yan Yuanlin Liu Xingguo Dai Zhongying Ma Yuanyuan Huang Qiyan He Pengbo Shen Guosheng Fu Tingyan Li Qiang 2014IMP & HIRFL Annual Report2014,,1:0
16Multifunctional solar-blind ultraviolet photodetectors based on p-PCDTBT/n-Ga_(2)O_(3)heterojunction with high photoresponse显示文摘Solar-blind ultraviolet(UV)photodetectors based on p-organic/n-Ga_(2)O_(3) hybrid heterojunctions have attracted extensive attention recently.Herein,the multifunctional solar-blind photodetector based on p-type poly[N-90-heptadecanyl-2,7-carbazole-alt-5,5-(40,70-di-2-thienyl-20,10,30-benzothiadiazole)](PCDTBT)/n-type amorphous Ga_(2)O_(3)(a-Ga_(2)O_(3))is fabricated and investigated,which can work in the phototransistor mode coupling with self-powered mode.With the introduction of PCDTBT,the dark current of such the a-Ga_(2)O_(3)-based photodetector is decreased to 0.48 pA.Meanwhile,the photoresponse parameters of the a-Ga_(2)O_(3)-based photodetector in the phototransistor mode to solar-blind UV light are further increased,that is,responsivity(R),photo-detectivity(D*),and external quantum efficiency(EQE)enhanced to 187 A W^(-1),1.3×10^(16) Jones and 9.1×10^(4)% under the weak light intensity of 11μW cm^(-2),respectively.Thanks to the formation of the built-in field in the p-PCDTBT/n-Ga_(2)O_(3) type-Ⅱ heterojunction,the PCDTBT/Ga_(2)O_(3) multifunctional photodetector shows self-powered behavior.The responsivity of p-PCDTBT/n-Ga_(2)O_(3) multifunctional photodetector is 57.5 mA W^(-1) at zero bias.Such multifunctional p-n hybrid heterojunction-based photodetectors set the stage for realizing high-performance amorphous Ga_(2)O_(3) heterojunction-based photodetectors.Yifei Wang Zhenhua Lin Jingli Ma Yongyi Wu Haidong Yuan Dongsheng Cui Mengyang Kang XingGuo Jie Su Jinshui Miao Zhifeng Shi Tao Li Jincheng Zhang Yue Hao Jingjing Chang 2024InfoMat2024,6,2:0
17Fungal diversity notes 1512–1610: taxonomic and phylogenetic contributions on genera and species of fungal taxa显示文摘This article is the 14th in the Fungal Diversity Notes series,wherein we report 98 taxa distributed in two phyla,seven classes,26 orders and 50 families which are described and illustrated.Taxa in this study were collected from Australia,Brazil,Burkina Faso,Chile,China,Cyprus,Egypt,France,French Guiana,India,Indonesia,Italy,Laos,Mexico,Russia,Sri Lanka,Thailand,and Vietnam.There are 59 new taxa,39 new hosts and new geographical distributions with one new combination.The 59 new species comprise Angustimassarina kunmingense,Asterina lopi,Asterina brigadeirensis,Bartalinia bidenticola,Bartalinia caryotae,Buellia pruinocalcarea,Coltricia insularis,Colletotrichum fexuosum,Colletotrichum thasutense,Coniochaeta caraganae,Coniothyrium yuccicola,Dematipyriforma aquatic,Dematipyriforma globispora,Dematipyriforma nilotica,Distoseptispora bambusicola,Fulvifomes jawadhuvensis,Fulvifomes malaiyanurensis,Fulvifomes thiruvannamalaiensis,Fusarium purpurea,Gerronema atrovirens,Gerronema favum,Gerronema keralense,Gerronema kuruvense,Grammothele taiwanensis,Hongkongmyces changchunensis,Hypoxylon inaequale,Kirschsteiniothelia acutisporum,Kirschsteiniothelia crustaceum,Kirschsteiniothelia extensum,Kirschsteiniothelia septemseptatum,Kirschsteiniothelia spatiosum,Lecanora immersocalcarea,Lepiota subthailandica,Lindgomyces guizhouensis,Marthe asmius pallidoaurantiacus,Marasmius tangerinus,Neovaginatispora mangiferae,Pararamichloridium aquisubtropicum,Pestalotiopsis piraubensis,Phacidium chinaum,Phaeoisaria goiasensis,Phaeoseptum thailandicum,Pleurothecium aquisubtropicum,Pseudocercospora vernoniae,Pyrenophora verruculosa,Rhachomyces cruralis,Rhachomyces hyperommae,Rhachomyces magrinii,Rhachomyces platyprosophi,Rhizomarasmius cunninghamietorum,Skeletocutis cangshanensis,Skeletocutis subchrysella,Sporisorium anadelphiae-leptocomae,Tetraploa dashaoensis,Tomentella exiguelata,Tomentella fuscoaraneosa,Tricholomopsis lechatii,Vaginatispora favispora and Wetmoreana blastidiocalcarea.The new combination is Torula sundara.The 39 new records on hosts and geographical distribution comprise Apiospora guiyangensis,Aplosporella artocarpi,Ascochyta medicaginicola,Astrocystis bambusicola,Athelia rolfsii,Bambusicola bambusae,Bipolaris luttrellii,Botryosphaeria dothidea,Chlorophyllum squamulosum,Colletotrichum aeschynomenes,Colletotrichum pandanicola,Coprinopsis cinerea,Corylicola italica,Curvularia alcornii,Curvularia senegalensis,Diaporthe foeniculina,Diaporthe longicolla,Diaporthe phaseolorum,Diatrypella quercina,Fusarium brachygibbosum,Helicoma aquaticum,Lepiota metulispora,Lepiota pongduadensis,Lepiota subvenenata,Melanconiella meridionalis,Monotosporella erecta,Nodulosphaeria digitalis,Palmiascoma gregariascomum,Periconia byssoides,Periconia cortaderiae,Pleopunctum ellipsoideum,Psilocybe keralensis,Scedosporium apiospermum,Scedosporium dehoogii,Scedosporium marina,Spegazzinia deightonii,Torula fci,Wiesneriomyces laurinus and Xylaria venosula.All these taxa are supported by morphological and multigene phylogenetic analyses.This article allows the researchers to publish fungal collections which areimportant for future studies.An updated,accurate and timely report of fungus-host and fungus-geography is important.We also provide an updated list of fungal taxa published in the previous fungal diversity notes.In this list,erroneous taxa and synonyms are marked and corrected accordingly.Ruvishika S.Jayawardena Kevin D.Hyde Song Wang Ya‑Ru Sun Nakarin Suwannarach Phongeun Sysouphanthong Mohamed A.Abdel‑Wahab Faten A.Abdel‑Aziz Pranami D.Abeywickrama Vanessa P.Abreu Alireza Armand AndréAptroot Dan‑Feng Bao Dominik Begerow Jean‑Michel Bellanger Jadson D.P.Bezerra Digvijayini Bundhun Mark S.Calabon Ting Cao Taimy Cantillo João LVRCarvalho Napalai Chaiwan Che‑Chih Chen Régis Courtecuisse Bao‑Kai Cui Ulrike Damm Cvetomir M.Denchev Teodor T.Denchev Chun Y.Deng Bandarupalli Devadatha Nimali Ide Silva Lidiane Ados Santos Nawal K.Dubey Sylvain Dumez Himashi SFerdinandez André L.Firmino Yusufon Gaforov Achala J.Gajanayake Deecksha Gomdola Sugantha Gunaseelan Shucheng‑He Zin H.Htet Malarvizhi Kaliyaperumal Martin Kemler Kezhocuyi Kezo Nuwan DKularathnage Marco Leonardi Ji‑Peng Li Chunfang Liao Shun Liu Michael Loizides Thatsanee Luangharn Jian Ma Hugo Madrid S.Mahadevakumar Sajeewa S.N.Maharachchikumbura Dimuthu S.Manamgoda María P.Martín Niranjan Mekala Pierre‑Arthur Moreau Yan‑Hong Mu Pasouvang Pahoua Dhandevi Pem Olinto L.Pereira Wiphawanee Phonrob Chayanard Phukhamsakda Mubashar Raza Guang‑Cong Ren Andrea C.Rinaldi Walter Rossi Binu C.Samarakoon Milan CSamarakoon Vemuri V.Sarma Indunil C.Senanayake Archana Singh Maria F.Souza Cristina M.Souza‑Motta Adriano A.Spielmann Wenxin Su Xia Tang XingGuo Tian Kasun M.Thambugala Naritsada Thongklang Danushka S.Tennakoon Nopparat Wannathes DingPeng Wei Stéphane Welti Subodini N.Wijesinghe Hongde Yang Yunhui Yang Hai‑Sheng Yuan Huang Zhang Jingyi Zhang Abhaya Balasuriya Chitrabhanu SBhunjun Timur S.Bulgakov Lei Cai Erio Camporesi Putarak Chomnunti Y.S.Deepika Mingkwan Doilom Wei‑Jun Duan Shi‑Ling Han Naruemon Huanraluek EBGareth Jones NLakshmidevi Yu Li Saisamorn Lumyong Zong‑Long Luo Surapong Khuna Jaturong Kumla Ishara S.Manawasinghe Ausana Mapook Wilawan Punyaboon Saowaluck Tibpromma Yong‑Zhong Lu JiYe Yan Yong Wang 2022Fungal Diversity2022,,6:0
18Genome-wide investigation of defensin genes in peanut(Arachis hypogaea L.)reveals AhDef2.2 conferring resistance to bacterial wilt显示文摘Peanut(Arachis hypogea L.)bacterial wilt(BW)is a devastating disease caused by Ralstonia solanacearum that results in severe yield and quality losses.Plant defensins are short cysteine-rich peptides with antimicrobial activity.The role of defensin genes(Ah Def)in peanut is unclear.A genome-wide investigation of Ah Def genes was undertaken,and 12 identified Ah Def genes were classified into two groups containing the gamma-thionin domain formed by four disulfide pairs:Cys1-Cys8,Cys2-Cys5,Cys3-Cys6,and Cys4-Cys7.In silico analysis revealed that Ah Def genes showed highly conserved architectural features and contained cis-elements associated with phytohormone signaling and defense responses.A highly resistant cultivar,H108(R)and a susceptible accession,H107(S)were tested by R.solanacearum inoculation.H108(R)showed fewer symptoms than H107(S)owing to inhibition of bacterial reproduction and spread in the vascular bundles of roots and stems.In a transcriptomic expression profile,Ah Def genes,particularly Ah Def1.6 and Ah Def2.2,were up-regulated in H108(R)compared with H107(S)under R.solanacearum infection and phytohormone treatment.Subcellular localization showed that the Ah Def1.6 and Ah Def2.2 proteins were both expressed specifically on the plasma membrane.Overexpression of protein fusion Ah Def2.2-YFP in Nicotiana benthamiana and peanut leaves increased resistance to R.solanacearum,suggesting its role in response to BW infection.Ah Def2.2 may be valuable for peanut resistance breeding.Kai Zhao Rui Ren Xingli Ma Kunkun Zhao Chengxin Qu Di Cao Qian Ma Yingying Ma Fangping Gong Zhongfeng Li Xingguo Zhang Dongmei Yin 2022The Crop Journal2022,10,3:0
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