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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein显示文摘Very recently,a novel coronavirus,2019-nCoV,emerged in Wuhan,China and then quickly spread worldwide,resulting in>17,388 confirmed cases and 361 deaths as of 3 February 2020,thus calling for the development of safe and effective therapeutics and prophylatics.1,2 Similar to severe acute respiratory syndrome(SARS)-CoV,2019-nCoV belongs to lineage B betacoronavirus,and it has the ability to utilize human angiotensin-converting enzyme 2(ACE2)as a receptor to infect human cells. | Shuai Xia Yun Zhu Meiqin Liu Qiaoshuai Lan Wei Xu Yanling Wu Tianlei Ying Shuwen Liu Zhengli Shi Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，7： | 27 |
| 3 | China' s energy development strategy under the low - carbon economy显示文摘 | Jiang Bing Sun Zhenqing Liu Meiqin | 2010 | Energy2010，35，11： | 1 |
| 4 | China’s energy development strategy under the low-carbon economy显示文摘 | Bing Jiang Zhenqing Sun Meiqin Liu | 2010 | Energy2010，，11： | 1 |
| 5 | China's energy develop- ment strategy under the low-carbon economy显示文摘 | Jiang Bing Zhenqing Sun Meiqin Liu | 2011 | Energy2011，35，11： | 1 |
| 6 | China's energy development strategy under the low-carbon economy 显示文摘 | Jiang Bing Zhenqing Sun Meiqin Liu | 2011 | Energy2011，35，11： | 1 |
| 7 | China's energy development strategy under the low-carbon economy 显示文摘 | Bing Jiang Zhenqing Sun Meiqin Liu | 2010 | Energy2010，，35： | 1 |
| 8 | Non-escaping frost tolerant QTL linked genetic loci at reproductive stage in six wheat DH populations显示文摘Reproductive stage frost poses a major constraint for wheat production in countries such as Australia.However,little progress has been made in identifying key genes to overcome the constraint.In the present study,a severe frost event hit two large-scale field trials consisting of six doubled haploid(DH)wheat populations at reproductive stage(young microspore stage)in Western Australia,leading to the identification of 30 robust frost QTL on 17 chromosomes.The major 18 QTL with the phenotype variation over 9.5%were located on 13 chromosomes including 2 A,2 B,2 D,3 A,4 A,4 B,4 D,5 A,5 D,6 D,7 A,7 B and7 D.Most frost QTL were closely linked to the QTL of anthesis,maturity,Zadok stages as well as linked to anthesis related genes.Out of those,six QTL were repetitively detected on the homologous regions on 2 B,4 B,4 D,5 A,5 D,7 A in more than two populations.Results showed that the frost damage is associated with alleles of Vrn-A1 a,Vrn-D1 a,Rht-B1 b,Rht-D1 b,and the high copy number of Ppd-B1.However,anthesis QTL and anthesis related genes of Vrn-B1 a and Ta FT3-1 B on chromosomes 5 B and 1 B did not lead to frost damage,indicating that these early-flowering phenotype related genes are compatible with frost tolerance and thus can be utilised in breeding.Our results also indicate that wild-type alleles Rht-B1 a and Rht-D1 a can be used when breeding for frost-tolerant varieties without delaying flowering time. | Jingjuan Zhang MD Shahidul Islam Yun Zhao Masood Anwar Zaid Alhabbar Maoyun She Rongchang Yang Angela Juhasz Guixiang Tang Jiansheng Chen Hang Liu Yanjie Jiang Shengnan Zhai Xin Hu Junkang Rong Yingquan Zhang Yebo Qin Qier Liu Zitong Yu Yujuan Zhang Sadegh Balotf Mirza Dowla Sonia Afrin Nandita Roy Md Resad Mallik Md Atik Us Saieed Shanjida Rahman Nigarin Sultana Sarah Al-Sheikh Ahmed Chris Florides Kefei Chen Darshan Sharma Nathan Height Ben Biddulph Meiqin Lu Jorge EMayer Wujun Ma | 2022 | The Crop Journal2022，10，1： | 0 |
| 9 | Using Petri nets to verify acyclic rule-based system显示文摘A series of Petri net-based definitions were formulated for describing four types of structural errors in a rule-based system(RBS),including inconsistency,incompleteness,redundancy and circularity.A marked v-Petri net model of acyclic RBS was constructed.Then,its reachability tree was generated to record all reachable relations between propositions in RBS.Moreover,a backward reasoning forest of a reachable marking was generated for explicitly representing reachable paths in RBS.Finally,a set of theorems and algorithms were provided to analyze and check structural errors.The usability of the research results presented in this paper was illustrated by an example. | Zhijun DING Meiqin PAN Changjun JIANG Yaojun HAN | 2008 | Frontiers of Electrical and Electronic Engineering in China2008，3，2： | 0 |
| 10 | 阳离子通道TRPV在醛缩酶和AMPK感知葡萄糖过程中起必须的作用显示文摘文章简介在激活AMPK的溶酶体途径感应葡萄糖供给下降的过程中,果糖-1,6-二磷酸醛缩酶(aldolase)起关键作用。然而,醛缩酶是如何把其与果糖-1,6-二磷酸结合的减少的信息传导引起AMPK活化的机制还尚未清楚。 | Mengqi Li Chen-Song Zhang Yue Zong Jin-Wei Feng Teng Ma Meiqin Hu Zhizhong Lin Xiaotong Li Changchuan Xie Yaying Wu Dong Jiang Ying Li Cixiong Zhang Xiao Tian Wen Wang Yanyan Yang Jie Chen Jiwen Cui Yu-Qing Wu Xin Chen Qing-Feng Liu Jianfeng Wu Shu-Yong Lin Zhiyun Ye Ying Liu Hai-Long Piao Li Yu Zhuan Zhou Xiao-Song Xie D.Grahame Hardie 林圣彩 | 2020 | 科学新闻2020，，2： | 0 |
| 11 | Nanostructure Architectures of Tungsten Carbide for Methanol Electrooxidation Catalyst显示文摘我们报导了一个新奇协议高效地综合有纯晶体结构和某些特定的形态学的钨碳化物(WC ) nanomaterials。在这个协议的关键步是催化剂先锋 W 18 有表面氧空缺的 O 49 。氧空缺的大数量的存在为散开给足够隧道并且把 WC 的特定的形态学放在碳化过程。与明确的几何形状造 WC 块的努力(例如,杆,电线,范围和薄片) 进一步扩展了它的可能性是对甲醇 electrooxidation 的支持催化剂。在纯阶段 WC 材料上支持的铂为甲醇氧化用作有效公司容忍的 electrocatalyst。电气化学的实验显示改进催化活动与 WC 支持的特殊形态学有关。它象特征一样的海顽童导致在 WC 之间的实质的接口,由准备更多的锚的磅为随后的水晶生长为装载的磅并且也削尖障碍,它提高在 WC 和磅之间的 synergistic 效果。 | Yekun Jiang Meiqin Shi Xue Tong Youqun Chu Chunan Ma | 2016 | Chinese Journal of Chemistry2016，34，6： | 0 |
| 12 | Human IFT-A complex structures provide molecular insights into ciliary transport显示文摘Intraflagellar transport(IFT)complexes,IFT-A and IFT-B,form bidirectional trains that move along the axonemal microtubules and are essential for assembling and maintaining cilia.Mutations in IFT subunits lead to numerous ciliopathies involving multiple tissues.However,how IFT complexes assemble and mediate cargo transport lacks mechanistic understanding due to missing highresolution structural information of the holo-complexes.Here we report cryo-EM structures of human IFT-A complexes in the presence and absence of TULP3 at overall resolutions of 3.0–3.9Å.IFT-A adopts a“lariat”shape with interconnected core and peripheral subunits linked by structurally vital zinc-binding domains.TULP3,the cargo adapter,interacts with IFT-A through its N-terminal region,and interface mutations disrupt cargo transport.We also determine the molecular impacts of disease mutations on complex formation and ciliary transport.Our work reveals IFT-A architecture,sheds light on ciliary transport and IFT train formation,and enables the rationalization of disease mutations in ciliopathies. | Meiqin Jiang Vivek Reddy Palicharla Darcie Miller Sun-Hee Hwang Hanwen Zhu Patricia Hixson Saikat Mukhopadhyay Ji Sun | 2023 | Cell Research2023，33，4： | 0 |
| 13 | Ablation of Zfhx4 results in early postnatal lethality by disrupting the respiratory center in mice显示文摘Breathing is an integrated motor behavior that is driven and controlled by a network of brainstem neurons.Zfhx4 is a zinc finger transcription factor and our results showed that it was specifically expressed in several regions of the mouse brainstem.Mice lacking Zfhx4 died shortly after birth from an apparent inability to initiate respiration.We also found that the electrical rhythm of brainstem‒spinal cord preparations was significantly depressed in Zfhx4-null mice compared to wild-type mice.Immunofluorescence staining revealed that Zfhx4 was coexpressed with Phox2b and Math1 in the brainstem and that Zfhx4 ablation greatly decreased the expression of these proteins,especially in the retrotrapezoid nucleus.Combined ChIP‒seq and mRNA expression microarray analysis identified Phox2b as the direct downstream target gene of Zfhx4,and this finding was validated by ChIP‒qPCR.Previous studies have reported that both Phox2b and Math1 play key roles in the development of the respiratory center,and Phox2b and Math1 knockout mice are neonatal lethal due to severe central apnea.On top of this,our study revealed that Zfhx4 is a critical regulator of Phox2b expression and essential for perinatal breathing. | Meiqin Zhang Sichen Du Huayuan Ou Renjie Cui Nan Jiang Yifeng Lin Runsheng Ge Duan Ma Jin Zhang | 2021 | Journal of Molecular Cell Biology2021，13，3： | 0 |
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