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| 1 | In Silico Screening for Microsatellite Markers from Expressed Sequence Tags of Porphyra yezoensis (Bangiales, Rhodophyta)显示文摘The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs) were utilized to identify simple sequence repeats (SSRs), or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1 162 out of 21 954 ESTs contained microsatellites and cluster analysis indi- cated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites. | WANG Mengqiang HU Jingjie ZHUANG Yunyun ZHANG Lei LILT Wei MAO Yunxiang | 2007 | Journal of Ocean University of China2007，6，2： | 8 |
| 2 | Binder-Free Activated Carbon/Carbon Nanotube Paper Electrodes for Use in Supercapacitors显示文摘新奇便宜、轻、灵活、平的 rollup 或便携设备为多功能的便携式的电子学被要求并且作为在当代的电池和 supercapacitors 使用的材料的选择开发新万用、灵活的电极材料是关键挑战。这里,为在先进 supercapacitors 的使用的没有文件夹的激活的碳(交流)/carbon nanotube (CNT ) 纸电极基于便宜、工业等级的排列 CNT 被制作了。由砍的一个二拍子的圆舞策略,排列 CNT 被驱散进单个长 CNT,然后交流的 90 wt%99 wt% 粉末被合并到 CNT 肉和 AC/CNT 纸电极被免职在一个过滤器上制作。AC/CNT 纸电极的特定的能力,率表演,和力量密度比对一个交流 / 乙炔黑人电极的相应价值好。电容到达了 267.6 F/g 的最大的价值, CNT 5 wt% 装载,并且精力密度和功率密度是 22.5 | Guanghui Xu Chao Zheng Qiang Zhang Jiaqi Huang Mengqiang Zhao Jingqi Nie Xianghua Wang Fei Wei | 2011 | Nano Research2011，4，9： | 6 |
| 3 | Cloning and Analysis of Calmodulin Gene from Porphyra yezoensis Ueda (Bangiales, Rhodophyta)显示文摘In order to understand the mechanisms of signal transduction and anti-desiccation mechanisms of Porphyra yezoensis, cDNA and its genomic sequence of Calmodulin gene (CaM) was cloned by the technique of polymerase chain reaction (PCR) based on the analysis of P. yezoensis ESTs from dbEST database. The result shows that the full-length cDNA of CaM consists of 603 bps including an ORF encoding for 151 amino acids and a terminate codon UGA, while the length of genomic sequence is 1231 bps including 2 exons and 1 intron. The average GC content of the coding region is 58.77%, while the GC content of the third position of this gene is as high as 82.23%. Four Ca2+ binding sites (EF-hand) are found in this gene. The predicted molecular mass of the deduced peptide is 16688.72 Da and the pI is 4.222. By aligning with known CaM genes, the similarity of CaM gene sequence with homologous genes in Chlamydomonas incerta and Chlamydomonas reinhardtii is 72.7% and 72.2% respectively, and the similarity of the deduced amino acid sequence of CaM gene with homologous genes in C. incerta and C. reinhardtii are both 71.5%. This is the first report on CaM from a species of Rhodophyta. | WANG Mengqiang MAO Yunxiang ZHUANG Yunyun KONG Fanna SUI Zhenghong | 2009 | Journal of Ocean University of China2009，8，3： | 3 |
| 4 | Fiber-tip polymer clamped-beam probe for high-sensitivity nanoforce measurements显示文摘Micromanipulation and biological,material science,and medical applications often require to control or measure the forces asserted on small objects.Here,we demonstrate for the first time the microprinting of a novel fiber-tip-polymer clamped-beam probe micro-force sensor for the examination of biological samples.The proposed sensor consists of two bases,a clamped beam,and a force-sensing probe,which were developed using a femtosecond-laser-induced two-photon polymerization(TPP)technique.Based on the finite element method(FEM),the static performance of the structure was simulated to provide the basis for the structural design.A miniature all-fiber micro-force sensor of this type exhibited an ultrahigh force sensitivity of 1.51 nmμN−1,a detection limit of 54.9 nN,and an unambiguous sensor measurement range of~2.9 mN.The Young’s modulus of polydimethylsiloxane,a butterfly feeler,and human hair were successfully measured with the proposed sensor.To the best of our knowledge,this fiber sensor has the smallest force-detection limit in direct contact mode reported to date,comparable to that of an atomic force microscope(AFM).This approach opens new avenues towards the realization of small-footprint AFMs that could be easily adapted for use in outside specialized laboratories.As such,we believe that this device will be beneficial for high-precision biomedical and material science examination,and the proposed fabrication method provides a new route for the next generation of research on complex fiber-integrated polymer devices. | Mengqiang Zou Changrui Liao Shen Liu Cong Xiong Cong Zhao Jinlai Zhao Zongsong Gan Yanping Chen Kaiming Yang Dan Liu Ying Wang Yiping Wang | 2021 | Light(Science & Applications)2021，10，9： | 3 |
| 5 | Design and realization of 3D printed fiber-tip microcantilever probes applied to hydrogen sensing显示文摘Cantilevers in microelectromechanical systems have the advantages of non-labeling,real-time detection,positioning,and specificity.Rectangular solid,rectangular hollow,and triangular microcantilevers were fabricated on an optical fiber tip via two-photon polymerization.The mechanical properties were characterized using finite element simulations.Coating the microcantilever with a palladium film enabled high sensitivity and rapid hydrogen detection.The shape of the cantilever determines the sensitivity,whereas the thickness of the palladium film determines the response time.Additional microelectromechanical systems can be realized via polymerization combined with optical fibers. | Changrui Liao Cong Xiong Jinlai Zhao Mengqiang Zou Yuanyuan Zhao Bozhe Li Peng Ji Zhihao Cai Zongsong Gan Ying Wang Yiping Wang | 2022 | Light(Advanced Manufacturing)2022，3，1： | 2 |
| 6 | 3D printed fiber-optic nanomechanical bioprobe显示文摘Ultrasensitive nanomechanical instruments,e.g.atomic force microscopy(AFM),can be used to perform delicate biomechanical measurements and reveal the complex mechanical environment of biological processes.However,these instruments are limited because of their size and complex feedback system.In this study,we demonstrate a miniature fiber optical nanomechanical probe(FONP)that can be used to detect the mechanical properties of single cells and in vivo tissue measurements.A FONP that can operate in air and in liquids was developed by programming a microcantilever probe on the end face of a single-mode fiber using femtosecond laser two-photon polymerization nanolithography.To realize stiffness matching of the FONP and sample,a strategy of customizing the microcantilever’s spring constant according to the sample was proposed based on structure-correlated mechanics.As a proof-of concept,three FONPs with spring constants varying from 0.421 N m^(−1)to 52.6 N m^(−1)by more than two orders of magnitude were prepared.The highest microforce sensitivity was 54.5 nmμN^(−1)and the detection limit was 2.1 nN.The Young’s modulus of heterogeneous soft materials,such as polydimethylsiloxane,muscle tissue of living mice,onion cells,and MCF-7 cells,were successfully measured,which validating the broad applicability of this method.Our strategy provides a universal protocol for directly programming fiber-optic AFMs.Moreover,this method has no special requirements for the size and shape of living biological samples,which is infeasible when using commercial AFMs.FONP has made substantial progress in realizing basic biological discoveries,which may create new biomedical applications that cannot be realized by current AFMs. | Mengqiang Zou Changrui Liao Yanping Chen Lei Xu Shuo Tang Gaixia Xu Ke Ma Jiangtao Zhou Zhihao Cai Bozhe Li Cong Zhao Zhourui Xu Yuanyuan Shen Shen Liu Ying Wang Zongsong Gan Hao Wang Xuming Zhang Sandor Kasas Yiping Wang | 2023 | International Journal of Extreme Manufacturing2023，5，1： | 1 |
| 7 | Roles of autophagy?related genes Beclin?1 and LC3 in the developmentand progression of prostate cancer and benign prostatic hyperplasia显示文摘 | Chengyi Liu Pengcheng Xu Degang Chen Xinhuan Fan Yipeng Xu Mengqiang Li Xu Yang Congfei Wang | 2013 | Biomedical Reports2013，，6： | 1 |
| 8 | Encrypted optical fiber tag based on encoded fiber Bragg grating array显示文摘Optical fibers are typically used in telecommunications services for data transmission,where the use of fiber tags is essential to distinguish between the different transmission fibers or channels and thus ensure the working functionality of the communication system.Traditional physical entity marking methods for fiber labeling are bulky,easily confused,and,most importantly,the label information can be accessed easily by all potential users.This work proposes an encrypted optical fiber tag based on an encoded fiber Bragg grating(FBG)array that is fabricated using a point-by-point femtosecond laser pulse chain inscription method.Gratings with different resonant wavelengths and reflectivities are realized by adjusting the grating period and the refractive index modulations.It is demonstrated that a binary data sequence carried by a fiber tag can be inscribed into the fiber core in the form of an FBG array,and the tag data can be encrypted through appropriate design of the spatial distributions of the FBGs with various reflection wavelengths and reflectivities.The proposed fiber tag technology can be used for applications in port identification,encrypted data storage,and transmission in fiber networks. | Zhihao Cai Bozhe Li Zhiyong Bai Dejun Liu Kaiming Yang Bonan Liu Cong Zhao Mengqiang Zou Jie Zhou Shangben Jiang Jingyi Huang Li Liu Xuming Zhang Junle Qu Yiping Wang Changrui Liao | 2023 | International Journal of Extreme Manufacturing2023，5，3： | 0 |
| 9 | Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene(FcδGST)from Chinese shrimp Fenneropenaeus chinensis显示文摘Glutathione S-transferases(GSTs)are a superfamily of multifunction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates.In the present study,a delta class GST gene(designated as FcδGST)was cloned from Fenneropenaeus chinensis using rapid amplification of c DNA ends(RACE)technology.The complete cDNA sequence of FcδGST was 780 bp in length,which includes a 27-bp 5′non-coding region(UTR),a 117-bp 3′UTR,a 636-bp open reading frame(ORF),and a polyadenylate signal site(AATAAA)presented at the upstream of poly A tail.The FcδGST gene encoded 211 amino acids peptide,including a GST_N domain and a GST_C domain,and exhibited high similarity with previously reported delta GSTs.The predicted molecular mass of FcδGST protein was 23.39 kDa,and its theoretical isoelectric point(pI)was 5.34.The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues,with the highest expression level in hemocytes and hepatopancreas.During the stimulation of Vibrio anguillarum or white spot syndrome virus(WSSV),the m RNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation.The purified recombinant FcδGST protein(designated as rFcδGST)exhibited specific catalytic activity against 1-chloro-2,4-dinitrobenzene(CDNB)substrate with relatively low stable enzymatic activities.These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of F.chinensis. | Jiaobing LI Yan WANG Jingjie HU Yajin XU Qingqian ZHOU Lu ZHANG Mengqiang WANG | 2023 | Journal of Oceanology and Limnology2023，41，5： | 0 |
| 10 | Na-K liquid alloy:A review on wettability enhancement and ionic carrier selection mechanism显示文摘The intrinsic liquid interface of Na-K alloy allays concerns about dendrite growth on metal anodes that are thermodynamically within the room temperature(20-22℃).Nevertheless,it hinders the formation of a stable electrode structure due to the inferior wettability induced by considerable liquid tension.In addition,the dominant ionic carrier in the Na-K alloy is subject to multiple factors,which is not conducive to customized battery design.This review,based on recently reported frontier achievements on Na-K liquid anodes,summarizes practical strategies for promoting the wettability by hightemperature induction,capillary effect,vacuum infiltration,and solid interface protection.Furthermo re,four selection mechanisms of the dominant ionic carrier are presented:(1)ion property dominated,(2)cathode dominated,(3)separator dominated,and(4)solid electrolyte interface dominated.Notably,initial electrolytes in energy storage systems have been unable to play a decisive role in ionic selection.Utilizing a superior wettability strategy and simultaneously identifying the dominant ionic carrier can facilitate the tailored application of dendrite-free Na-K liquid anodes. | Xinran Li Jiahao Liu Cheng Chen Jian Yang Ziqiang Xu Mengqiang Wu Yuesheng Wang Zaghib Karim | 2021 | Chinese Chemical Letters2021，32，3： | 0 |
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