|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Enhanced hydrogen production by insertional inactivation of adhE gene in Klebsiella oxytoca HP1显示文摘Ethanol is the main byproduct of anaerobic H2-producing fermentation in Klebsiella oxytoca HP1. Two moles of NAD(P)H are consumed to yield one mole of ethanol that may decrease bacterial hydrogen production. In this article the adhE gene that codes for acetaldehyde dehydrogenase was disrupted for the first time. A homologous recombination vector pTA-Str was constructed in which the adhE gene was disrupted by inserting an aminoglycoside-3'-adenyltransferase (aadA) gene. As expected, the vector includes the insertion 5′-adhE-aadA-adhE-3′. The amplified DNA fragment 5′-adhE-aadA-adhE-3′ from pTA-Str was transformed into K. oxytoca HP1 and one recombinant was obtained. PCR analysis of the resulting genomic DNA indicated the appropriate deletion and insertion. Compared with the H2-production of wild type K. oxytoca HP1, the hydrogen yield of the mutant increased by 16.07% and ethanol concentration decreased by 77.47%, suggesting that inactivation of the adhE gene in K. oxy- toca HP1 is a potential method for enhancing bacterial H2-production. | ZHU JunBo LONG MinNan XU Fangcheng WU XiaoBing XU HuiJuan | 2007 | Chinese Science Bulletin2007，52，4： | 2 |
| 2 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | Minnan L Jinli H Xiaobin W | 2005 | Res Microbiol2005，156，2： | 1 |
| 3 | Isolation and characterization of a high H2-producing strain Klebsialle oxytoca HPl from a hot spring显示文摘 | Minnan L Jinli H Xiaobin W | 2005 | Res Microbiol2005，156，： | 1 |
| 4 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | LONG Minnan HANG Jinli WU Xiaobin | 2005 | Research in Microbiology2005，156，1： | 1 |
| 5 | Isolation and characterization of a high H2-producing bacteria Klebsiella oxytoca HP1 from a hot spring显示文摘 | Minnan L Jinli H Xiaobin W | 2005 | Research in microbiology2005，156，2： | 1 |
| 6 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | Long Minnan Huang Jinli Wu Xiaobin | 2005 | Research in Microbiology2005，156，： | 1 |
| 7 | Turbulence Structure of Hydraulic Jumps of Low Froude Numbers显示文摘 | Liu Minnan Rajaratnam Nallamuthu Zhu D Z | 2004 | Journal of Hydraulic Engineering2004，130，6： | 1 |
| 8 | Mean flow and turbulence structure in vertical slot fishway 显示文摘 | Liu Minnan Nallamuthu R David Z Zhu 2006 | 2006 | Journal of Hydraulics Engineering2006，132，8： | 1 |
| 9 | Effect of carboxylate multi-walled carbon nanotubes on the performance of thermoplastic starch nanocomposites 显示文摘 | LIU Zhanjun ZHAO Lei CHEN Minnan | 2011 | Carbohydr Polym2011，83，： | 1 |
| 10 | Mean flow and turbulence structure in vertical slot fishways显示文摘 | Liu Minnan Rajaramam N David Z | 2006 | Journal of Hydraulic Engineering2006，132，8： | 1 |
| 11 | Screening and Characterization of the High-Cellulase-Producing Strain Aspergillus glaucus XC9显示文摘Cellulose is a kind of renewable resource that is abundant in nature.It can be degraded by microorganisms such as mildew.A mildew strain with high cellulase activity was isolated from mildewy maize cob and classified as Aspergillus glaucus XC9 by morphological and 18S rRNA gene sequence analyses.We studied the effects of nitrogen source,initial pH,temperature,incubation time,medium composition,and surfactants on cellulase production.Maximal activities of carboxymethylcellulase(6,812 U/g dry koji)and filter paperase(172 U/g dry koji)were obtained in conditions as follows:initial pH,5.5–6.0;temperature,30℃;cultivation period,3–4 days;inoculum ratio,6%(vol/vol);sugarcane bagasse/wheat bran ratio,4:6.When bagasse was used as substrate and mixed with wet koji at a 1:1(wt/wt)ratio,the yield of reducing sugars was 36.4%.The corresponding conversion rate of cellulose to reducing sugars went as high as 81.9%.The results suggest that A.glaucus XC9 is a preferred candidate for cellulase production. | Xu Chang Long Minnan Wu Xiaobing Xu Huijuan Chen Zhongan Zhang Fengzhang Xu Liangshu | 2006 | Frontiers in Biology2006，1，1： | 1 |
| 12 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | Minnan L Jinli H Xiaobin W | 2004 | Research in Microbiology2004，156，1： | 1 |
| 13 | Mean flow and turbulence structure in vertical slot fishway 显示文摘 | LIU Minnan Nallamuthu R David Z Zhu | 2006 | Journal of Hydraulics Engineering2006，132，8： | 1 |
| 14 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | L Minnan H Jinli W Xiaobin | 2005 | Research in Microbiology2005，156，1： | 1 |
| 15 | Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring显示文摘 | Minnan L Jinli H Xiaobin W | 2005 | Research in Microbiology2005，156，： | 1 |
| 16 | Isolation and characterization of a high H2-production strain Klebsiella oxytoea HP1 from a hot spring显示文摘 | L Minnan H Jinli W Xiaohin | 2005 | Research in Microbiology2005，156，1： | 1 |
| 17 | Clinical value of ^(18)F-FDG PET/CT in assessing suspicious relapse after rectal cancer resection显示文摘AIM:To evaluate the value of 18F-fluorodeoxyglucose positron emission tomography/computed tomography(18F-FDG PET/CT) in the restaging of resected rectal cancer.METHODS:From January 2007 to Sep 2008,21 patients who had undergone curative surgery resection for rectal carcinoma with suspicious relapse in conventional imaging or clinical findings were retrospectively enrolled in our study.The patients underwent 28 PET/CT scans(two patients had two scans,one patient had three and one had four scans).Locoregional recurrences and/or distant metastases were confirmed by histological analysis or clinical and imaging follow-up.RESULTS:Final diagnosis was confirmed by histopathological diagnosis in 12 patients(57.1) and by clinical and imaging follow-up in nine patients(42.9).Eight patients had extrapelvic metastases with no evidence of pelvic recurrence.Seven patients had both pelvic recurrence and extrapelvic metastases,and two patients had pelvic recurrence only.18F-FDG PET/CT was negative in two patients and positive in 19 patients.18F-FDG PET/CT was true positive in 17 patients and false positive in two.The accuracy of 18F-FDG PET/CT was 90.5,negative predictive value was 100,and positive predictive value was 89.5.Five patients with perirectal recurrence underwent 18F-FDG PET/CT image guided tissue core biopsy.18F-FDG PET/CT also guided surgical resection of pulmonary metastases in three patients and monitored the response to salvage chemotherapy and/or radiotherapy in four patients.CONCLUSION:18F-FDG PET/CT is useful for evaluating suspicious locoregional recurrence and distant metastases in the restaging of rectal cancer after curative resection. | Long Sun,Wei-Min Pan,Zuo-Ming Luo,Ji-Hong Wei,Long Zhao,Hua Wu,Minnan PET Center and Department of Nuclear Medicine,the First Hospital of Xiamen University,Xiamen 316003,Fujian Province,China Yong-Song Guan,State Key Laboratory of Biotherapy,West China Medical School,Sichuan University,Gaopeng Street,Keyuan Road 4,Chengdu 610041,Sichuan Province,China | 2009 | World Journal of Gastrointestinal Oncology2009，1，1： | 1 |
| 18 | Mean flow and turbulence structure in vertical slot fishway显示文摘 | Liu Minnan Rajaratnam Nallamuthu Zhu David Z | | 0，，08： | 1 |
| 19 | Isolation and charac- terization of a high 1-12-producing strain Klebsiella oxytoca HP1 from:a hot spring 显示文摘 | Minnan L Jinli H Xiaobin W | 2005 | Research in Microbiology2005，156，1： | 1 |
| 20 | Characterization of a HoxEFUYH type of hydrogenase from Allochromatium vinosum and some EPR and IR properties of the hydrogenase module显示文摘 | Minnan L Jingjing L Zhifeng C | 2007 | J Biol Inorg Chem2007，12，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员