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| 1 | Jiangtang Xiaoke granule attenuates glucose metabolism disorder via regulating endoplasmic reticulum stress in the liver of type 2 diabetes mellitus mice显示文摘OBJECTIVE:To observe the effect of Jiangtang Xiaoke(JTXK) granule on endoplasmic reticulum(ER)stress in high fat diet(HFD)-induced type 2 diabetes mellitus(T2 DM) KK-Ay mice.METHODS:KK-Ay mice were fed with HFD to induce the T2 DM model,while normal control C57 BL/6 J mice were given standard feed.Fasting blood glucose(FBG) in all mice was measured weekly and oral glucose tolerance tests(OGTTs) were performed at 4 and 10 weeks after start of treatment to determine glucose metabolism.Serum fasting insulin(FINS) and insulin sensitivity index(ISI) were measured to determine insulin sensitivity.m RNA expressions of eukaryotic initiation factor-2 alpha(e IF2α),glucose regulated protein 78(GRP78),activating transcription factor 4(ATF4),and C/EBP homology protein(CHOP) were assessed by reverse transcription polymerase chain reaction and the protein expressions of p-e IF2α,GRP78,and CHOP were assessed by Western blotting.RESULTS:JTXK granule significantly reduced FBG and free fatty acid levels and improved OGTT at the120 min of the 10-week treatment in T2 DM KK-Ay mice.FINS and Hb Alc levels were reduced and insulin sensitivities were increased in KK-Ay diabetic mice,which were improved with the treatment of JTXK granule,especially at the 7 and 3.5 g/kg doses.JTXK granule at the 3.5 g/kg dose was most effective in reducing both gene and protein expressions of e IF2α,GRP78,and CHOP.CONCLUSION:ER stress response is increased in T2 DM KK-Ay mice.Treatment with JTXK granule attenuates glucose disorders,improves insulin sensitivity,and reduces serum FFA in T2 DM KK-Ay mice.The mechanisms may be attributed to regulation of the signaling ER stress pathway via decreasing e IF2α phosphorylation and suppressing e IF2α-ATF4-CHOP activation. | Zhang Yi Mo Fangfang Zhang Dongwei Gao Sihua Zhao Dandan Yu Na Mu Qianqian Zuo Jiacheng Ma Yue | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 3 |
| 2 | Exposure to Prorocentrum minimum Induces Oxidative Stress and Apoptosis in the Ridgetail White Prawn, Exopalaemon carinicauda显示文摘Prorocentrum minimum is a bloom-forming, planktonic mixotrophic dinoflagellate, and can cause stress in shrimp ponds. In this study, healthy Exopalaemon carinicauda were exposed to 5 × 10^4 cells mL^-1 P. minimum for 72 hours to investigate the adverse effect of P. minimum on shrimps. Elevated superoxide dismutase(SOD) activity and malondialdehyde(MDA) content, reduced total antioxidant capacity(T-AOC) and catalase(CAT) activity, and regulatory glutathione peroxidase(GPX) activity were found in the hemolymph of E. carinicauda after exposure to P. minimum. In this study, P. minimum exposure induced oxidative stress and caused significant oxidative damage to E. carinicauda. P. minimum exposure increased the expression of HSP70 gene in the hemocyte, gills and hepatopancreas. Compared with the enhanced level of caspase-3 gene mR NA in the hemocyte and gills, the up-regulation of caspase-3 gene in the hepatopancreas was only observed from 3 to 6 h, and then the mRNA level of glutathione-S-transferase(GST) gene increased. These results indicated that GST might be involved in the shrimp hepatopancreas’ defense against P. minimum exposure. The present study demonstrates that exposure to P. minimum could induce oxidative stress and apoptosis in E. carinicauda. The SOD activity, HSP70 and GST(in the hepatopancreas) were evoked to protect cells from oxidative stress and apoptosis. This study will provide new insights into the toxic mechanism of P. minimum on shrimps. | MU Cuimin GE Qianqian LI Jian | 2019 | Journal of Ocean University of China2019，18，3： | 2 |
| 3 | Antioxidant Response of Ridgetail White Prawn Exopalaemon carinicauda to Harmful Dinoflagellate Prorocentrum minimum Exposure and Its Histological Change显示文摘The dinoflagellate Prorocentrum minimum,one of the most widespread red tide causing species,affects marine aquaculture and ecosystems worldwide.In this study,ridgetail white prawn Exopalaemon carinicauda were exposed to P.minimum cells(5 × 104 cells mL-1) to investigate its harmful effects on the shrimp.Antioxidant activities and histological changes were used as indicators of health status of the shrimp.In 72 hours,the mortality of E.carinicauda was not affected,but its antioxidant response and histology were statistically different from those of control.Elevated superoxide dismutase(SOD) and glutathione peroxidase(GPX) activities and depressed catalase(CAT) activity were observed in gill;while increased SOD,glutathione S-transferase(GST),CAT activities and modulated GPX activity were observed in hepatopancreas.Thus,antioxidant activities in gill and hepatopancreas seem to respond differentially to harmful alga exposure.Increased malondialdehyde(MDA) content in early a few hours indicates the damage of the antioxidant defense system.Although MDA content recovered to a low level thereafter,a series of histological abnormalities including accumulation or infiltration of hemocytes,tissue lesions and necrosis were discovered in gill and hepatopancreas.Exposure to P.minimum induced sublethal effects on E.carinicauda,including temporary oxidative damage and histological injury. | MU Cuimin REN Xianyun GE Qianqian WANG Jiajia LI Jian | 2017 | Journal of Ocean University of China2017，16，2： | 2 |
| 4 | Ginsenoside rg3 reduces body weight by regulating fat content and browning in obese mice显示文摘Objective:To determine the effects of ginsenoside rg3 on the body weight of C57BL/6J obese mice and to investigate its underlying weight loss mechanisms with a focus on white fat browning-related factors.Methods:Eight-week-old C57BL/6J male mice were fed a high-fat diet for 12 successive weeks to construct the obese model.C57BL/6J male mice were fed a standard chow diet to construct normal control group.After 8 weeks of intervention with ginsenoside rg3,the food intake,body weight,body fat mass,blood sugar,and lipid profiles of the mice in each group were detected.Hematoxylin and eosin(HE)staining was used to observe the histological morphology of the adipose tissues.Real-time polymerase chain reaction(RT-PCR)and Western blotting(WB)were applied to detect the gene and protein expression levels of peroxisome proliferators-activated receptor gama(PPARg),Peroxisome proliferatoractivated receptor-gamma coactivator-1alpha(PGC-1a),PR domain containing 16(PRDM16),and uncoupling protein 1(UCP-1).Results:Compared to normal control group mice,the body weight,food intake,body fat composition,and blood lipid levels of model group mice increased significantly.After 8 weeks of intervention with ginsenoside rg3,body weight,body fat composition,food intake,and blood lipid profiles decreased.HE staining showed that ginsenoside rg3 can improve white adipocyte hypertrophy to a certain extent.RTPCR and WB demonstrated that ginsenoside rg3 can increase the mRNA and protein expression levels of PPARg,PGC-1a,PRDM16,and UCP-1 in the adipose tissues of obese mice.Conclusion:The weight reduction effect of ginsenoside rg3 may be related to the promotion of white fat browning. | Qianqian Mu Jiacheng Zuo Dandan Zhao Xiaoshan Zhou Jing Hua Ying Bai Fangfang Mo Xin Fang Min Fu Sihua Gao | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，1： | 0 |
| 5 | Photochemical production of carbonyl sulfide,carbon disulfide and dimethyl sulfide in a lake water显示文摘Photochemical production of carbonyl sulfide(COS),carbon disulfide(CS_2) and dimethyl sulfide(DMS) was intensively studied in the water from the Aohai Lake of Beijing city.The lake water was found to be highly supersaturated with COS,CS_2 and DMS,with their initial concentrations of 0.91 ± 0.073 nmol/L,0.55 ± 0.071 nmol/L and 0.37 ± 0.062 nmol/L,respectively.The evident photochemical production of COS and CS_2 in the lake water under irradiation of 365 nm and 302 nm indicated that photochemical production of them might be the reason for their supersaturation.The similar dependence of wavelength and oxygen for photochemical production of COS,CS_2 and DMS implied that they might be from the same precursors.The water cage effect was found to favor COS production but inhibit CS_2and DMS formation,indicating that COS photochemical production was mainly from direct degradation of the precursors and the formation of CS_2 and DMS needed intermediates via combination of carbon-centered radicals and sulfur-centered radicals.The above assumptions were further confirmed by simulation experiments with addition of carbonyls and amino acids(cysteine and methionine),and the photochemical formation mechanisms for COS,CS_2 and DMS in water were derived from the investigations. | Qianqian Du Yujing Mu Chenglong Zhang Junfeng Liu Yuanyuan Zhang Chengtang Liu | 2017 | Journal of Environmental Sciences2017，29，1： | 0 |
| 6 | Dynamic Resource Pricing and Allocation in Multilayer Satellite Network显示文摘The goal of delivering high-quality service has spurred research of 6G satellite communication networks.The limited resource-allocation problem has been addressed by next-generation satellite communication networks,especially multilayer networks with multiple low-Earth-orbit(LEO)and nonlow-Earth-orbit(NLEO)satellites.In this study,the resource-allocation problem of a multilayer satellite network consisting of one NLEO and multiple LEO satellites is solved.The NLEO satellite is the authorized user of spectrum resources and the LEO satellites are unauthorized users.The resource allocation and dynamic pricing problems are combined,and a dynamic gamebased resource pricing and allocation model is proposed to maximize the market advantage of LEO satellites and reduce interference between LEO and NLEO satellites.In the proposed model,the resource price is formulated as the dynamic state of the LEO satellites,using the resource allocation strategy as the control variable.Based on the proposed dynamic game model,an openloop Nash equilibrium is analyzed,and an algorithm is proposed for the resource pricing and allocation problem.Numerical simulations validate the model and algorithm. | Yuan Li Jiaxuan Xie Mu Xia Qianqian Li Meng Li Lei Guo Zhen Zhang | 2021 | Computers, Materials & Continua2021，，12： | 0 |
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