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| 1 | 利用基于扫描相机的荧光寿命成像显微技术研究细胞周期显示文摘利用基于扫描相机的荧光寿命成像显微系统,以细胞周期为模型,研究转染绿色荧光蛋白的HeLa细胞的荧光寿命。结果表明,处于周期内不同进程的细胞的荧光寿命为2.50-3.00 ns。处于分裂期的细胞的荧光寿命在1 h内从2.86 ns下降到2.82 ns;在DNA合成前期的8 h内,荧光寿命从2.82 ns下降到2.78 ns。荧光寿命的差异反映了细胞周期中核浆内大分子浓度的变化,对了解细胞周期的分子机制有一定的意义。 | 王岩 赵羚伶 陈同生 许改霞 Artem Pliss Tymish Y.Ohulchanskyy Paras N.Prasad 屈军乐 牛憨笨 | 2011 | 中国激光2011，38，3： | 7 |
| 2 | Black phosphorus-based photothermal therapy with aCD47-mediated immune checkpoint blockade for enhanced cancer immunotherapy显示文摘Here,we describe a combination strategy of black phosphorus(BP)-based photothermal therapy together with anti-CD47 antibody(aCD47)-based immunotherapy to synergistically enhance cancer treatment.Tumour resistance to immune checkpoint blockades in most cancers due to immune escape from host surveillance,along with the initiation of metastasis through immunosuppressive cells in the tumour microenvironment,remains a significant challenge for cancer immunotherapy.aCD47,an agent for CD47/SIRPαaxis blockade,induces modest phagocytic activity and a low response rate for monotherapy,resulting in failures in clinical trials.We showed that BP-mediated ablation of tumours through photothermal effects could serve as an effective strategy for specific immunological stimulation,improving the inherently poor immunogenicity of tumours,which is particularly useful for enhancing cancer immunotherapy.BP in combination with aCD47 blockade activates both innate and adaptive immunities and promotes local and systemic anticancer immune responses,thus offering a synergistically enhanced effect in suppression of tumour progression and in inducing abscopal effects for inhibition of metastatic cancers.Our combination strategy provides a promising platform in which photothermal agents could help to enhance the therapeutic efficacy of immunotherapy. | Zhongjian Xie Minhua Peng Ruitao Lu Xiangying Meng Weiyuan Liang Zhongjun Li Meng Qiu Bin Zhang Guohui Nie Ni Xie Han Zhang Paras N.Prasad | 2020 | Light(Science & Applications)2020，9，1： | 3 |
| 3 | Hot-band absorption of indocyanine green for advanced anti-stokes fluorescence bioimaging显示文摘Bright anti-Stokes fluorescence(ASF)in the first near-infrared spectral region(NIR-I,800 nm–900 nm)under the excitation of a 915 nm continuous wave(CW)laser,is observed in Indocyanine Green(ICG),a dye approved by the Food and Drug Administration for clinical use.The dependence of fluorescence intensity on excitation light power and temperature,together with fluorescence lifetime measurement,establish this ASF to be originated from absorption from a thermally excited vibrational level(hot-band absorption),as shown in our experiments,which is stronger than the upconversion fluorescence from widely-used rare-earth ion doped nanoparticles.To test the utility of this ASF NIR-I probe for advanced bioimaging,we successively apply it for biothermal sensing,cerebral blood vessel tomography and blood stream velocimetry.Moreover,in combination with L1057 nanoparticles,which absorb the ASF of ICG and emit beyond 1100 nm,these two probes generate multi-mode images in two fluorescent channels under the excitation of a single 915 nm CW laser.One channel is used to monitor two overlapping organs,urinary system&blood vessel of a live mouse,while the other shows urinary system only.Using in intraoperative real-time monitoring,such multi-mode imaging method can be beneficial for visual guiding in anatomy of the urinary system to avoid any accidental injury to the surrounding blood vessels during surgery. | Jing Zhou Xiaoxiao Fan Di Wu Jie Liu Yuhuang Zhang Zikang Ye Dingwei Xue Mubin He Liang Zhu Zhe Feng Andrey N.Kuzmin Wen Liu Paras N.Prasad Jun Qian | 2021 | Light(Science & Applications)2021，10，10： | 3 |
| 4 | In-situ neutron-transmutation for substitutional doping in 2D layered indium selenide based phototransistor显示文摘Neutron-transmutation doping(NTD)has been demonstrated for the first time in this work for substitutional introduction of tin(Sn)shallow donors into two-dimensional(2D)layered indium selenide(InSe)to manipulate electron transfer and charge carrier dynamics.Multidisciplinary study including density functional theory,transient optical absorption,and FET devices have been carried out to reveal that the field effect electron mobility of the fabricated phototransistor is increased 100-fold due to the smaller electron effective mass and longer electron life time in the Sn-doped InSe.The responsivity of the Sn-doped InSe based phototransistor is accordingly enhanced by about 50 times,being as high as 397 A/W.The results show that NTD is a highly effective and controllable doping method,possessing good compatibility with the semiconductor manufacturing process,even after device fabrication,and can be carried out without introducing any contamination,which is radically different from traditional doping methods. | Zhinan Guo Yonghong Zeng Fanxu Meng Hengze Qu Shengli Zhang Shipeng Hu Sidi Fan Haibo Zeng Rui Cao Paras N.Prasad Dianyuan Fan Han Zhang | 2022 | eLight2022，2，1： | 1 |
| 5 | Next generation lanthanide doped nanoscintillators and photon converters显示文摘Scintillators are of significance for the realization of indirect X-ray detection and X-ray excited optical luminescence(XEOL)imaging.However,commercial bulk scintillators not only require complex fabrication procedures,but also exhibit non-tunable XEOL wavelength and poor device processability.Moreover,thick crystals usually generate light scattering followed by evident signal crosstalk in a photodiode array.Lanthanide doped fluoride nanoscintillators(NSs)prepared with low-temperature wet-chemical method possess several advantages,such as low toxicity,cheap fabrication cost,convenient device processability and adjustable emission wavelengths from ultraviolet to visible and extending to second near infrared window.In addition,they exhibit X-ray excited long persistent luminescence(XEPL)making them suitable for broadening the scope of their applications.This review discusses and summarizes the XEOL and XEPL characteristics of lanthanide doped fluoride NSs.We discuss design strategies and nanostructures that allow manipulation of excitation dynamics in a core-shell geometry to simultaneously produce XEOL,XEPL,as well as photon upconversion and downshifting,enabling emission at multiple wavelengths with a varying time scale profile.The review ends with a discussion of the existing challenges for advancing this field,and presents our subjective insight into areas of further multidisciplinary opportunities. | Lei Lei Yubin Wang Andrey Kuzmin Youjie Hua Jingtao Zhao Shiqing Xu Paras N.Prasad | 2022 | eLight2022，2，1： | 1 |
| 6 | Broadband mid-infrared nonlinear optical modulator enabled by gold nanorods:towards the mid-infrared regime显示文摘Mid-infrared pulsed lasers operating around the 3 μm wavelength regime are important for a wide range of applications including sensing, spectroscopy, imaging, etc. Despite the recent advances in technology, the lack of a nonlinear optical modulator operating in the mid-infrared regime remains a significant challenge. Here, we report the third-order nonlinear optical response of gold nanorods(GNRs) ranging from 800 nm to the mid-infrared regime(2810 nm) enabled by their size and overlapping behavior-dependent longitudinal surface plasmon resonance. In addition, we demonstrate a wavelength-tunable Er3+-doped fluoride fiber laser modulated by GNRs, which can deliver pulsed laser output, with the pulse duration down to 533 ns, tunable wavelength ranging from 2760.2 to 2810.0 nm, and spectral 3 d B bandwidth of about 1 nm. The experimental results not only validate the GNRs’ robust mid-infrared nonlinear optical response, but also manifest their application potential in high-performance broadband optoelectronic devices. | BIN HUANG ZHE KANG JIE LI MINGYI LIU PINGHUA TANG LILI MIAO CHUJUN ZHAO GUANSHI QIN WEIPING QIN SHUANGCHUN WEN PARAS N.PRASAD | 2019 | Photonics Research2019，7，6： | 0 |
| 7 | Uptake of transferrin-conjugated quantum dots in single living cells显示文摘We study the uptake and distribution of transferrin (Tf)-conjugated CdSe/CdS/ZnS quantum dots (QDs) in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional (3D) reconstruction technique. By increasing the co-incubation time or the dosage of QDs-Tf, we find that the uptake of QDs-Tf bioconjugates in the cells increases correspondingly, but with different uptake rates. Additionally, the distribution of QDs-Tf, in single live HeLa cells is time dependent. To our knowledge, this is the first study on quantitatively analyzing the uptake and distribution of bioconjugated QDs in single living cells. Such QDs nanoplatform can be further modified for developing biomedical evaluation tool in cancer diagnosis and targeted drug delivery. | 陈丹妮 许改霞 Bahi Ahmed Ali Ken-Tye Yong 周翠红 王晓梅 屈军乐 Paras N.Prasad 牛憨笨 | 2010 | Chinese Optics Letters2010，8，10： | 0 |
| 8 | Water‑Dispersible CsPbBr_(3) Perovskite Nanocrystals with Ultra‑Stability and its Application in Electrochemical CO_(2) Reduction显示文摘Thanks to the excellent optoelectronic properties,lead halide perovskites(LHPs)have been widely employed in highperformance optoelectronic devices such as solar cells and lightemitting diodes.However,overcoming their poor stability against water has been one of the biggest challenges for most applications.Herein,we report a novel hot-injection method in a Pb-poor environment combined with a well-designed purification process to synthesize water-dispersible CsPbBr_(3) nanocrystals(NCs).The as-prepared NCs sustain their superior photoluminescence(91%quantum yield in water)for more than 200 days in an aqueous environment,which is attributed to a passivation effect induced by excess CsBr salts.Thanks to the ultra-stability of these LHP NCs,for the first time,we report a new application of LHP NCs,in which they are applied to electrocatalysis of CO_(2) reduction reaction.Noticeably,they show significant electrocatalytic activity(faradaic yield:32%for CH4,40%for CO)and operation stability(>350 h). | Keqiang Chen Kun Qi Tong Zhou Tingqiang Yang Yupeng Zhang Zhinan Guo Chang‑Keun Lim Jiayong Zhang Igor Zutic Han Zhang Paras N.Prasad | 2021 | Nano-Micro Letters2021，13，11： | 0 |
| 9 | Fluorescence lifetime imaging for studying DNA compaction and gene activities显示文摘Optical imaging is a most useful and widespread technique for the investigation of the structure and function of the cellular genomes.However,an analysis of immensely convoluted and irregularly compacted DNA polymer is highly challenging even by modern super-resolution microscopy approaches.Here we propose fluorescence lifetime imaging(FLIM)for the advancement of studies of genomic structure including DNA compaction,replication as well as monitoring of gene expression.The proposed FLIM assay employs two independent mechanisms for DNA compaction sensing.One mechanism relies on the inverse quadratic relation between the fluorescence lifetimes of fluorescence probes incorporated into DNA and their local refractive index,variable due to DNA compaction density.Another mechanism is based on the Förster resonance energy transfer(FRET)process between the donor and the acceptor fluorophores,both incorporated into DNA.Both these proposed mechanisms were validated in cultured cells.The obtained data unravel a significant difference in compaction of the gene-rich and gene-poor pools of genomic DNA.We show that the gene-rich DNA is loosely compacted compared to the dense DNA domains devoid of active genes. | Svitlana M.Levchenko Artem Pliss Xiao Peng Paras N.Prasad Junle Qu | 2021 | Light(Science & Applications)2021，10，12： | 0 |
| 10 | Light-induced tumor theranostics based on chemical-exfoliated borophene显示文摘Among 2D materials(Xenes)which are at the forefront of research activities,borophene,is an exciting new entry due to its uniquely varied optical,electronic,and chemical properties in many polymorphic forms with widely varying band gaps including the lightest 2D metallic phase.In this paper,we used a simple selective chemical etching to prepare borophene with a strong near IR light-induced photothermal effect.The photothermal efficiency is similar to plasmonic Au nanoparticles,with the added benefit of borophene being degradable due to electron deficiency of boron.We introduce this selective chemical etching process to obtain ultrathin and large borophene nanosheets(thickness of ~4 nm and lateral size up to ~600 nm)from the precursor of AlB_(2).We also report first-time observation of a selective Acid etching behavior showing HCl etching of Al to form a residual B lattice,while HF selectively etches B to yield an Al lattice.We demonstrate that through surface modification with polydopamine(PDA),a biocompatible smart delivery nanoplatform of B@PDA can respond to a tumor environment,exhibiting an enhanced cellular uptake efficiency.We demonstrate that borophene can be more suitable for safe photothermal theranostic of thick tumor using deep penetrating near IR light compared to gold nanoparticles which are not degradable,thus posing longterm toxicity concerns.With about 40 kinds of borides,we hope that our work will open door to more discoveries of this top-down selective etching approach for generating borophene structures with rich unexplored thermal,electronic,and optical properties for many other technological applications. | Zhongjian Xie Yanhong Duo Taojian Fan Yao Zhu Shuai Feng Chuanbo Li Honglian Guo Yanqi Ge Shakeel Ahmed Weichun Huang Huiling Liu Ling Qi Rui Guo Defa Li Paras N.Prasad Han Zhang | 2022 | Light(Science & Applications)2022，11，12： | 0 |
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