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| 1 | Quantitative analysis of vascular endothelial growth factor, microvascular density and their clinicopathologic features in human hepatocellular carcinoma显示文摘BACKGROUND: Angiogenesis is known to be essential to the survival, growth, invasion, and metastasis of tumor cells. Vascular endothelial growth factor (VEGF) are an important angiogenic factor regulating tumor angiogenesis, but its significance and tumor pathologic features are un- clear in hepatocellular carcinoma (HCC). In the present study, we analyzed expression of tissue VEGF, alteration of microvascular density (MVD) in microvessel angiogenesis, development and metastasis of HCC, and level of serum VEGF in differential diagnosis of benign and malignant liv- er diseases. METHODS: Tumor specimens were prospectively collected from HCC patients undergoing resection. Total RNAs were extracted and the expression levels were detected from different parts of HCC tissues. The cellular distributions of VEGF and MVD of liver tumors and their paracancerous and distal cancerous tissues were investigated by streptavi- din peroxidase (S-P) immunohistochemistry, respectively. The VEGF levels of circulating blood and hepatoma tissues were measured by enzyme-linked immunosorbent assay. RESULTS: The incidence of VEGF expression was 63.9% in HCCs (23/36 cases), 78.3% in non-encapsulated HCCs (18/23), and 90.9% in HCCs with extrahepatic metastasis (10/11), respectively. The VEGF expression was tightly correlated with MVD (P <0.01). The MVD in HCC with metastasis, low differentiation or non-encapsulation was significantly higher than that in HCC with intact capsule, high differentiation, or no metastasis. No significant diffe- rence was found between VEGF, MVD, tumor size, and hepatitis virus infection. The level of total RNA in HCC tis- sues was significantly lower but the VEGF level significantly higher than those in paracancerous or distal cancerous ones (P<0.01). The abnormal expression levels of VEGF in sera of HCC patients were directly correlated with the me- tastasis and recurrence of tumors. CONCLUSION: The high expression of VEGF and abnor- mality of tissue MVD are useful predictors for vascular inva- sion and metastasis of liver tumors. | Deng-Fu Yao, Xin-Hua Wu, Yong Zhu, Gong-Sheng Shi, Zhi-Zhen Dong, Deng-Bing Yao, Wei Wu, Li-Wei Qiu and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology , Department of Pathology and Department of Gastroenterology , Affiliated Hos- pital of Nantong University Department of Diagnostics , and Institute of Neurosciences , Nantong University Nantong 226001, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 81 |
| 2 | Expression and alteration of insulin-like growth factor II-messenger RNA in hepatoma tissues and peripheral blood of patients with hepatocellular carcinoma显示文摘AIM: To investigate the clinical values of serum free insulin-like growth factor Ⅱ (IGF-Ⅱ) levels and IGF-Ⅱ mRNA in hepatocellular carcinoma (HCC) tissues and peripheral blood for diagnosis of HCC and monitoring of extrahepatic metastasis.METHODS: Total RNAs were extracted from HCC tissues or peripheral blood mononuclear cells from patients with HCC, liver diseases devoid of cancer, non-hepatic tumors,and healthy controls, respectively. IGF-Ⅱ cDNAs were synthesized through random primers and reversetranscriptase, amplified by polymerase chain reaction (PCR), and confirmed by DNA sequencing analysis. Serum free IGF-Ⅱ levels in patients with different liver diseases were analyzed by an enzyme-linked immunosorbent assay.RESULTS: The amplified fragments of IGF-Ⅱ mRNA by RT-PCR were identical to originally designed ones with a size of 170 bp and confirmed by sequencing analysis.The dilution experiments revealed that the lowest sensitivity of our system was 2 ng/L of total RNA. The positive frequencies of IGF-Ⅱ mRNA were 100% in HCC tissues,53.3% in para-cancerous tissues, and 0% in non-cancerous tissues, respectively. The serum free IGF-Ⅱ levels were significantly higher in HCC than those in chronic hepatitis or liver cirrhosis. The positive frequency of circulating IGF-Ⅱ mRNA was 34.2% in HCC, no amplified fragment was found in other liver diseases, extrahepatic tumors,and normal controls, respectively. The circulating IGF-Ⅱ mRNA correlated with the stage of HCC, and its positive rate was 100% in HCC with extrahepatic metastasis and 35.5% in HCC with AFP-negative. No significant correlation was found between tumor sizes and circulating IGF-Ⅱ mRNA fragment.CONCLUSION: The abnormal expressions of free IGF-Ⅱ and IGF-Ⅱ mRNA are useful tumor markers for HCC diagnosis, differentiation of extrahepatic metastasis and monitoring postoperative recurrence. | Zhi-Zhen Dong Deng-Fu Yao Deng-Bing Yao Xin-Hua Wu Wei Wu Li-Wei Qiu Dao-Rong Jiang Jian-Hua Zhu Xian-Yong Meng | 2005 | World Journal of Gastroenterology2005，11，30： | 45 |
| 3 | Clinical impact of plasma TGF-β1 and circulating TGF-β1 mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND:Transforming growth factor-β(TGF-β) plays an important role in the regulation of cell growth and differentiation,angiogenesis,extracellular matrix formation,immunosuppression and cancer development. In this study,we investigated the levels of TGF-β1 and TGF-β1 mRNA expression,their relationship with HBV replication,and their diagnostic value for hepatocellular carcinoma(HCC). METHODS:Total RNAs were extracted from HCC samples and matched non-tumor tissues,and from peripheral blood mononuclear cells in HCC patients.TGF-β1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-β1 expression was assessed by immunohistochemistry.The clinical characteristics were analyzed between TGF-β1 and HBV replication.The diagnostic value of circulating TGF-β1 and TGF-β1 mRNA levels were investigated in HCC patients. RESULTS:The incidence of hepatic TGF-β1 expression was 83.3%in HCC samples,43.3%in the surrounding tissues, 94.7%in the HBV DNA-positive group,and 63.6%in the HBV DNA-negative group.Liver TGF-β1 expression was associated with the degree of HCC differentiation and the status of HBV replication,but not with the size or number of tumors.Circulating TGF-β1 level and incidence of TGF-β1 mRNA were significantly higher in the HCC groupthan in any group of patients with benign liver disease, with a higher sensitivity of 89.5%and a specificity of 94.0% for HCC diagnosis when circulating TGF-β1 levels were >1.2μg/L.No significant correlation was found between TGF-β1 expression and AFP level or tumor size.Combining TGF-β1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS:Abnormal expression of hepatic TGF-β1 is associated with the degree of HCC differentiation and HBV replication.Both circulating TGF-β1 and TGF-β1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC. | Zhi-Zhen Dong,Deng-Fu Yao,Min Yao,Li-Wei Qiu,Lei Zong,Wei Wu, Xin-Hua Wu,Deng-Bing Yao and Xian-Yong Meng Department of Diagnostics,Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of GastroenterologyAffiliated Hospital and Institute of Neurosciences,Nantong University,Nantong 226001,China | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，3： | 27 |
| 4 | Cardiotoxicity of 5-fluorouracil and capecitabine in Chinese patients: a prospective study显示文摘Background:5-Fluorouracil(5-FU)and capecitabine-associated cardiotoxicity ranging from asymptomatic electro-cardiography(ECG)abnormalities to severe myocardial infarction has been reported in a number of studies,but such cardiotoxicity in Chinese patients with malignant diseases has not been investigated to date.In the present study,we aimed to prospectively evaluate the incidence rates and clinical manifestations of 5-FU-and capecitabine-associated cardiotoxicity in cancer patients recruited from multiple centers in China.Methods:Among the 527 patients who completed the study,196 received 5-FU-based chemotherapy and 331 received capecitabine-based chemotherapy as either first-line or adjuvant therapy.Adverse events were reported during the treatment and up to 28 days of follow-up.Outcome measures included ECG,myocardial enzymes,cardiac troponin,brain natriuretic peptide and echocardiography.Univariate analysis and logistic regression were performed for subgroup analysis and identification of significant independent variables that are associated with cardiotoxicity of both agents.Results:In total,161 of 527 patients(30.6%)experienced cardiotoxicity.The incidence rate of cardiotoxicity was 33.8%(112/331)in the capecitabine group,which was significantly higher than the rate of 25%(49/196)in the 5-FU group(P=0.0042).110/527 patients(20.9%)suffered arrhythmia,105/527(19.9%)developed ischemic changes,while only 20/527 patients(3.8%)presented heart failure and 6/527 patients(1.1%)had myocardial infarction.Pre-existing cardiac disease,hypertension,capecitabine-based chemotherapy and duration of treatment were identified as sig-nificant risk factors associated with cardiotoxicity.The odds ratio were 15.7(prior history of cardiac disease versus no history),1.86(capecitabine versus 5-FU),1.06(5-8 versus 1-4 chemotherapy cycles)and 1.58(hypertension versus no hypertension),respectively.Conclusions:Cardiotoxicity induced by fluoropyrimidines in the Chinese population may be underestimated in clini-cal practice.Close monitoring of patients is recommended,especially for those patients at high risk for cardiotoxicity.Possible risk factors are duration of treatment,capecitabine-based chemotherapy,pre-existing cardiac diseases and hypertension.Trial registration This study was initiated on January 22,2014 and has been retrospectively registered with the registra-tion number ChiCTR1800015434 . | Jianjun Peng Chao Dong Chang Wang Weihua Li Hao Yu Min Zhang Qun Zhao Bo Zhu Jun Zhang Wenliang Li Fenghua Wang Qiong Wu Wenhao Zhou Ying Yuan Meng Qiu Gong Chen | 2018 | Cancer Communications2018，38，1： | 23 |
| 5 | NIR-induced highly sensitive detection of latent finger- marks by NaYF4:Yb,Er upconversion nanoparticles in a dry powder state显示文摘通常在犯罪场面发现 fingermarks 的大多数是潜伏的并且,这样为检测潜伏的 fingermarks 的一个有效方法是很重要的。然而,传统的发展中技术有象低察觉敏感,高背景干扰,复杂操作,和高毒性那样的缺点。处理这挑战,我们采用了荧光灯 NaYF 4:Yb,Er upconversion nanoparticles (UCNP ) 它能发荧光可见的光什么时候由 980 nm 使对人安全的在红外线附近的光激动,到污点,各种各样的底层上的潜伏的 fingermarks 出现。 UCNP 成功地被用作一篇小说荧光灯为有包括非渗入的材料(玻璃,大理石,铝合金表,不锈钢表,铝陪衬,和塑料卡片)的各种各样的底层上的高敏感,低背景,高效率,和低毒性的潜伏的 fingermarks 的察觉的标签,渗入半的材料(地板皮革,陶器的瓦,木头地板,和油漆木头),和象报纸的各种各样的类型那样的渗透的材料。这个工作证明 UCNP 是一万用荧光灯为几乎任何东西上的 fingermarks 的灵巧的察觉的标签材料,在法庭的科学启用他们的实际应用。 | Meng Wang Ming Li Mingying Yang Xiaomei Zhang Aoyang Yu Ye Zhu Penghe Qiu Chuanbin Mao | 2015 | Nano Research2015，8，6： | 20 |
| 6 | A review of the development of full cell lithium-ion batteries: The impact of nanostructured anode materials显示文摘锂离子电池为消费者电子学市场作为最好的便携式的精力存储设备出现了。在 lithiumion 电池的开发的最近的进步被选择阳极材料的使用获得了,它以能力,周期的稳定性,和率能力在性能驾驶了改进。在这方面,研究集中于设计和完整的房间锂离子电池的电气化学的性能,最新利用开发了阳极材料,广泛地被报导了,并且在开发的大迈进被做了。Nanostructured 阳极材料主要作出贡献到完整的房间锂离子电池的发展。与这在头脑,我们在硬币房间的表演总结 nanostructured 阳极材料的影响完整的锂离子电池。这评论也讨论对完整的房间锂离子电池的研究的挑战和前景。 | Muhammad-Sadeeq Balogun Weitao Qiu Yang Luo Hui Meng Wenjie Mai Amos Onasanya Titus K. Olaniyi Yexiang Tong | 2016 | Nano Research2016，9，10： | 18 |
| 7 | Abnormal expression of hepatoma- derived γ-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes显示文摘BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer. | Deng-Fu Yao, Zhi-Zhen Dong, Deng-Bing Yao, Xin-Hua Wu, Wei Wu, Li-Wei Qiu, Hong-Mei Wang and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology, Affilia- ted Hospital of Nantong University, Nantong 226001 , China Insti- tute of Neuroscicnces, Nantong University, Nantong 226001 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 17 |
| 8 | The X-linked mental retardation gene PHF8 is a histone demethylase involved in neuronal differentiation显示文摘最近的研究在 PHF8 识别了变化,编码 JmjC 的 X 连接基因包含域的蛋白质,为 X 连接智力迟钝(XLMR ) 的一个原因的因素并且劈开 lip/cleft 腭。然而,内在的机制是未知的。这里,我们证明 PHF8 为 retinoic 酸受体(RAR ) 是 histone demethylase 和激活剂。尽管为 H3K4me3/2/1 和 H3K9me2/1 demethylation 的活动在细胞底的试金被检测, recombinant PHF8 在 vitro 展出了仅仅 H3K9me2/1 demethylase 活动,建议 PHF8 是其特性可以在 vivo 被调制的 H3K9me2/1 demethylase。重要地,在 XLMR 病人识别的变异的 PHF8 (在到丝氨酸的位置 279 点的本氨基丙酸) 在酶的活动是有缺点的,显示 histone demethylase 活动的损失有原因地与疾病的发作被连接。另外,我们证明 PHF8 经由一个 N 终端哲学博士手指领域明确地绑在 H3K4me3/2 肽。与为在 neuronal 区别的 PHF8 的一个角色一致,在鼠标的 PHF8 击倒胚胎的癌 P19 房间损害导致 RA 的 neuronal 区别,而 overexpression 野类型然而并非向 neuronal 区别的 F279S 变异的 PHF8 驱动器 P19 房间。而且,我们证明 PHF8 为 RAR 伪作为激活剂与 RAR 伪和功能交往。一起拿,我们的结果建议 PHF8 调制的 histone methylation 在 neuronal 区别起一个关键作用。 | Jihui Qiu Guang Shi Yuanhui Jia Jing Li Meng Wu Jiwen Li Shuo Dong Jiemin Wong | 2010 | Cell Research2010，20，8： | 16 |
| 9 | Ustilaginoidea virens: A Fungus Infects Rice Flower and Threats World Rice Production显示文摘Rice false smut disease, which is caused by the fungus Ustilaginoidea virens, is currently one of the most devastating rice fungal diseases in the world. Rice false smut disease not only causes severe yield loss and grain quality reduction, but also threatens food safety due to its production of mycotoxins. In this review, the most recent progresses regarding the life cycle, infection processes, genome and genetic diversity, pathogenic gene and disease resistance in rice were summarized in order to provide theoretical basis for the control of U. virens. We also proposed some future directions and key questions that need to be addressed for a better understanding of the molecular mechanism that leads to rice false smut disease and the prospects for sustainable control of rice false smut. | QIU Jiehua MENG Shuai DENG Yizhen HUANG Shiwen KOU Yanjun | 2019 | Rice science2019，26，4： | 16 |
| 10 | Selective laser melting of NiTi alloy with superior tensile property and shape memory effect显示文摘It is a challenge to develop complex-shaped Ni Ti shape memory alloy parts by traditional processing methods, due to the poor machinability of Ni Ti alloy. It is reported that selective laser melting(SLM) of additive manufacturing could overcome this problem. However, the reported SLM-produced Ni Ti exhibits poor tensile ductility due to the inner defects and adverse unidirectional columnar grains from SLM process. In this work, the defect-less SLM-Ni Ti with nondirective columnar grains was fabricated by optimizing the intraformational laser scanning length and interformational laser scanning direction. The obtained lath-shaped SLM-Ni Ti sample exhibits tensile strain of 15.6%, more than twice of the reported maximum result(7%). Besides, the SLM-Ni Ti part with complex geometry displays a shape memory recovery of 99% under compressive deformation of 50%. | Zhiwei Xiong Zhonghan Li Zhen Sun Shijie Hao Ying Yang Meng Li Changhui Song Ping Qiu Lishan Cui | 2019 | Journal of Materials Science & Technology2019，35，10： | 15 |
| 11 | Abnormal expression of HSP gp96 associated with HBV replication in human hepatocellular carcinoma显示文摘BACKGROUND: Heat shock protein (HSP) gp96 is a member of the HSP90 family and presumably overexpresses as a result of stimulation by mutated or abnormal proteins. Its abnormal expression correlates with carcinogenesis, progression and prognosis of hepatocellular carcinoma (HCC). In this study, we investigated the pathological characteristics of liver gp96 expression and its relationship with hepatitis B virus (HBV) replication in HCC patients. METHODS: Tumor specimens were prospectively collected from 30 HCC patients undergoing liver resection. Total RNAs were extracted from HCC or their noncancerous tissues. The distribution of gp96 expression in hepatocytes was investigated by streptavidin peroxidase (S-P) immunohistochemistry and tissue HBV-DNA was detected by the in situ molecular hybridization technique. The association of gp96 expression with HBV replication, and the histopathological characteristics of HCC were analyzed. RESULTS: The gp96 was strongly expressed in HCC (73.3%, 22 of 30) and weakly (46.7%, 14 of 30) in non-cancerous tissues. The gp96 expression in HCC tissues was correlated with degree of tumor differentiation and tumor size, but not with tumor number (P>0.05). Immunohistochemical analysis showed that 17 of 19 HCC patients with HBVDNA -positive were strongly expressed for gp96, whereas only 5 of 11 patients with HBV-DNA-negative were positive for gp96. A significant difference was found between the two groups (89.5% vs. 45.5%, P<0.05). CONCLUSIONS: The abnormal expressions of HSP gp96 in HCC tissues are associated with HBV replication. This finding indicates that HBV infection plays an important role in the development of HCC. | Deng-Fu Yao,Xin-Hua Wu,Xiao-Qin Su,Min Yao, Wei Wu, Li-Wei Qiu, Li Zou and Xian-Yong Meng The Institute of Clinical Medicine, Nantong University, and Department of Gastroenterology,Affiliated Hospital of Nantong University, Nantong 226001, China and Nanjing Medical University, Nanjing 210009, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，3： | 14 |
| 12 | Establishment and preliminery use of hepatitis Bvirus preS1/2 antigen assay显示文摘 | CHEN Kun, HAN Bao Guang, MA Xian Kai, ZHANG He Qiu, MENG Li, WANG Guo Hua, XIA Fang, SONG Xiao Guo and LING Shi Gan | 1999 | World Journal of Gastroenterology1999，5，6： | 14 |
| 13 | Toripalimab combined with lenvatinib and GEMOX is a promising regimen as first-line treatment for advanced intrahepatic cholangiocarcinoma:a single-center,single-arm,phase 2 study显示文摘Advanced intrahepatic cholangiocarcinoma(ICC)has a dismal prognosis.Here,we report the efficacy and safety of combining toripalimab,lenvatinib,and gemcitabine plus oxaliplatin(GEMOX)as first-line therapy for advanced ICC.Thirty patients with pathologically confirmed advanced ICC received intravenous gemcitabine(1 g/m2)on Days 1 and 8 and oxaliplatin(85 mg/m2)Q3W for six cycles along with intravenous toripalimab(240 mg)Q3W and oral lenvatinib(8 mg)once daily for one year.The expression of programmed death-ligand 1(PD-L1)and genetic status was investigated in paraffin-embedded tissues using immunohistochemistry and whole-exome sequencing(WES)analysis.The primary endpoint was the objective response rate(ORR).Secondary outcomes included safety,overall survival(OS),progression-free survival(PFS),disease control rate(DCR)and duration of response(DoR).As of July 1,2022,the median follow-up time was 23.5 months,and the ORR was 80%.Twenty-three patients achieved partial response,and one achieved complete response.Patients(21/30)with DNA damage response(DDR)-related gene mutations showed a higher ORR,while patients(14/30)with tumor area positivity≥1(PD-L1 staining)showed a trend of high ORR,but without significant difference.The median OS,PFS,and DoR were 22.5,10.2,and 11.0 months,respectively.The DCR was 93.3%.Further,56.7%of patients experienced manageable grade≥3 adverse events(AEs),commonly neutropenia(40.0%)and leukocytopenia(23.3%).In conclusion,toripalimab plus lenvatinib and GEMOX are promising first-line regimens for the treatment of advanced ICC.A phase-III,multicenter,double-blinded,randomized study to validate our findings was approved by the National Medical Products Administration(NMPA,No.2021LP01825). | Guo-Ming Shi Xiao-Yong Huang Dong Wu Hui-Chuan Sun Fei Liang Yuan Ji Yi Chen Guo-Huan Yang Jia-Cheng Lu Xian-Long Meng Xin-Ying Wang Lei Sun Ning-Ling Ge Xiao-Wu Huang Shuang-Jian Qiu Xin-Rong Yang Qiang Gao Yi-Feng He Yang Xu Jian Sun Zheng-Gang Ren Jia Fan Jian Zhou | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 12 |
| 14 | The single nucleotide polymorphisms of chicken melanocortin-4 receptor(MC4R)gene and their association analysis with carcass traits显示文摘Mutations of the melanocortin-4 receptor (MC4R) gene are associated with the appetite,obesity and growth in pig,mice and human. But little is known about the function of chicken MC4R gene. In this study,F2 chicken resource population derived from broilers crossing to Silky was screened for the polymorphisms of the MC4R gene using PCR-single strand conformation polymor-phism (PCR-SSCP) and DNA sequencing methods. Four single nucleotide polymorphisms (SNPs) sites were found. The mutation (C→T) in the 5′ regulation region of chicken MC4R gene results in one more NF-E2 and cap transcription factor binding sites in the mutation allele than in the wild allele. One missense mutation (G→A) occurs in the coding region (61nt),which changes the glycine to arginine. Moreover,in the coding region there are 2 synonymous mutations,one G→T mutation at 315nt and one C→T mutation at 336nt. Least square analysis of the SNPs and carcass traits showed that BB,DD and FF genotypes are significantly associated with body weight,carcass weight (or half carcass weight),and leg muscle weight (P<0.05 or P<0.01). But no significant association between the geno-types and abdominal fat weight is found. The results present the evidence that the chicken MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight and growth. | QIU Xuemei1,2,LI Ning1,DENG Xuemei3,ZHAO Xingbo3,MENG Qingyong1 & WANG Xiuli2 1. State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China 2. College of Life Science and Technology,Dalian Fisheries University,Dalian,116023,China 3. College of Animal Science and Technology,China Agricultural University,Beijing 100094,China | 2006 | Science China(Life Sciences)2006，49，6： | 12 |
| 15 | Controlling angular dispersions in optical metasurfaces显示文摘Although metasurfaces have shown great potential for manipulating light,most previously realized meta-devices suffer from uncontrolled angular dispersions,making them unfavorable for many applications.Here,we propose a general strategy to realize optical metasurfaces with desired angular dispersions based on carefully controlling both the near-field couplings between meta-atoms and the radiation pattern of a single meta-atom.Utilizing such a strategy,we experimentally demonstrate a series of optical meta-devices with predesigned angular dispersions,including two incident-angle-insensitive absorbers,one incident-angle-selective absorber,and one multifunctional meta-polarizer whose functionality changes from a perfect mirror to a half-waveplate as the excitation angle varies.Finally,we design a gradient meta-device using meta-atom arrays with purposely controlled angular dispersions and numerically demonstrate that it can exhibit distinct wavefront-control functionalities when illuminated at different incident angles.Our findings establish a new platform for achieving angle-multiplexed functional meta-devices,significantly expanding the wave-manipulation capabilities of optical metasurfaces. | Xiyue Zhang Qi Li Feifei Liu Meng Qiu Shulin Sun Qiong He Lei Zhou | 2020 | Light(Science & Applications)2020，9，1： | 11 |
| 16 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 17 | Metal-organic framework-derived, Zn-doped porous carbon polyhedra with enhanced activity as bifunctional catalysts for rechargeable zinc-air batteries显示文摘锌空气电池最近与他们的锂离子对应物相比由于更大的存储能力和更低的费用吸引了可观的兴趣。为氧减小反应(ORR ) 和氧进化反应(OER ) 的电极催化剂在可充电的锌空气电池的操作起一个关键作用。此处,我们报导简单、可伸缩的策略作为先锋用做 Zn 的共同基于的 zeolitic imidazolate 框架(ZnCo-ZIFs ) 制作多孔的碳 polyhedra。惊人地,做的 Zn 导致更小的公司 nanoparticles 和更高的氮内容,它接着提高获得的多孔的碳 polyhedra 的 ORR 和 OER 活动。做 N 的碳和钴 nanoparticles 在的 synergistic 效果合成,源于碳的高 graphitization 的改进传导性,和多孔的多面的结构的大表面区域在碱的媒介与优秀 ORR 和 OER electrocatalytic 活动导致了多孔的碳 polyhedra。更重要地,空气阴极在新窗户中在主要、可充电的锌空气 batteries.Open 图象基于最佳的多孔的碳 polyhedra 推进展出优异性能到 Pt/C | Xuan Wu Ge Meng Wenxian Liu Tian Li Qiu Yang Xiaoming Sun Junfeng Liu | 2018 | Nano Research2018，11，1： | 10 |
| 18 | Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield显示文摘Artemisia annua is the major natural source of artemisinin,an anti-malarial medicine commonly used worldwide.Here,we present chromosome-level haploid maps for two A.annua strains with different artemisinin contents to explore the relationships between genomic organization and artemisinin production.High-fidelity sequencing,optical mapping,and chromatin conformation capture sequencing were used to assemble the heterogeneous and repetitive genome and resolve the haplotypes of A.annua.Approximately 5o,ooo genes were annotated for each haplotype genome,and a triplication event that occurred approximately 58.12million years ago was examined for the first time in this species.A total of 3,903,467-5,193,414 variants(SNPs,indels,and structural variants)were identified in the 1.5-Gb genome during pairwise comparison between haplotypes,consistent with the high heterozygosity of this species.Genomic analyses revealed a correlation between artemisinin concents and the copy number of amorpha-4,11-dienes ynthasegenes.This correlation was further confirmed by resequencing of 36A.annua samples with varied artemisinin contents.Circular consensus sequencing of transcripts facilitated the detection of paralog expression.Collectively,our study provides chromosome-level allele-aware genome assemblies for two A.annua strains and new insights into the biosynthesis of artemisinin and its regulation,which will contribute to conquering malaria worldwide. | Baosheng Liao Xiaofeng Shen Li Xiang Shuai Guo Shiyu Chen Ying Meng Yu Liang Dandan Ding Junqi Bai Dong Zhang Tomasz Czechowski Yi Li Hui Yao Tingyu Mai Caroline Howard Chao Sun Haitao Liu Jiushi Liu Jin Pei Jihai Gao Jigang Wang Xiaohui Qiu Zhihai Huang Hongyi Li Ling Yuan Jianhe Wei lan Graham Jiang Xu Boli Zhang Shilin Chen | 2022 | Molecular Plant2022，15，8： | 9 |
| 19 | Microstructures and tensile properties of Mg-Zn-(Gd)-Zr alloys extruded at various temperatures显示文摘The microstructures and tensile properties of Mg-6.0 Zn-0.6 Zr(ZK60) and ZK60 + 1.8 Gd(ZEK620(RE = Gd)) alloys extruded at 290, 305, 320, 340 and360 ℃ were investigated. The results indicate that Gd addition can refine the microstructures of ZK60 alloy and the extrusion parameters such as the extrusion temperature will influence the tensile properties of both as-extruded ZK60 and ZEK620 alloys, although it is much slighter for ZK60 alloy. And the sensitivity is closely related to the textures developed during extrusion. In addition, Gd addition will also clearly improve the tensile strength at room temperature(24 ℃) and particularly at high temperatures(150 and 200 ℃), due to the much finer grains and smaller intermetallic particles. The highest strength is achieved in the as-extruded ZEK620 alloy with extrusion temperature of 320 ℃, with ultimate tensile strength(UTS) and tensile yield strength(TYS) of 405 and375 MPa, respectively, which are improved by approximately 9% and 11% than those of the as-extruded ZK60 alloy which owns the highest strength at extrusion temperature of 290 ℃. | Xin Qiu Qiang Yang Kai Guan Fanqiang Bu Zhan-Yi Cao Yong-Bing Liu Jian Meng | 2017 | Rare Metals2017，36，12： | 9 |
| 20 | Dynamic alteration of telomerase expression and its diagnostic significance in liver or peripheral blood for hepatocellular carcinoma显示文摘瞄准:在肝纸巾或外部血在肝细胞癌(HCC ) 和它的诊断含意的开发期间调查 telomerase 表示的动态改变为 HCC 的单音的原子房间。方法:肝 telomerase 的动态表情在 hepatocytes 的恶意的转变期间被观察在(SD ) Sprague-Dawly,老鼠用 0.05% 2-fluoenyacetamide (2-FAA ) 喂了。全部的 RNA 和 telomerase 从老鼠或人的肝纸巾被提取。在肝并且在传播的血的 telomerase 活动被 telomeric 重复扩大 protocol-enzyme-linked 免疫吸着剂试金(TRAP-ELISA ) 检测,并且它的诊断价值与良性或恶意的肝疾病在病人被调查。结果:肝细胞瘤模型在 HCC 开发期间显示了肝的 telomerase 的动态表示。telomerase 活动与肝总数 RNA 层次一致(r = 0.83, P<0.01 ) 在 hepatocytes 的退化,初癌状态,和癌化的阶段。在 HCC 病人,在 HCC 纸巾的 telomerase 层次比在他们的邻近的非癌的纸巾,而是肝全部的 RNA 铺平显著地高比在后者在前者是更低的。尽管 HCC 病人的传播 telomerase 反常地与长期的肝疾病在病人之中被表示,因为发生在正常控制是15.7%, telomerase 活动是为 HCC 诊断的一个非特定的标记,25%在长期的肝炎,45.9%在肝肝硬化,并且85.2%在 HCC ,分别地吸收度什么时候 telomerase 活动珍视,是超过 0.2 。如果价值是超过 0.6,发生在 HCC 组是 60% 并且 0% 在除了在有肝的二种情况中的其它(P<0.01 ) 的任何东西肝硬化。然而,有浆液 alpha-fetoprotein 水平的传播 telomerase 的联合能增加积极的率和精确性(92.6% , 135 中的 125 个) HCC 诊断。结论:过去 telomerase 的表示在肝纸巾与 HCC 开发,和它的反常被联系或在外设,血能是为 HCC 的诊断和预后的一个有用标记。 | Deng-Fu Yao Wei Wu Min Yao Li-Wei Qiu Xin-Hua Wu Xiao-Qin Su Li Zou Deng-Bing Yao Xian-Yong Meng | 2006 | World Journal of Gastroenterology2006，12，31： | 9 |
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