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| 1 | The role of charged multivesicular body protein 5 in programmed cell death in leukemic cells显示文摘人(CHMP5 ) 现代人的控告的 multivesicular 身体蛋白质 5 是 multivesicular 身体的一个成员,它用作 anti-apoptotic 蛋白质并且被认为参予引起白血病。在这研究,一条短发卡的基于 RNA 的 RNA 干扰途径被用来在白血病的房间线 U937 禁止 CHMP5 的表示。在 CHMP5 被禁止以后,抗体 microarray 和西方的污点分析被用来在规划房间死亡(PCD ) 学习变化小径。PCD 能被分类进三种类型:apoptosis,坏死,和 autophagy。结果显示出那 caspase 3 在 CHMP5 缺乏的 U937 房间被激活,显示 apoptotic 小径被激活,尽管既不内在外来的 apoptotic 小径也没被激活。我们的结果也证明 Granzyme B/Perforin apoptotic 小径被 CHMP5 silencing 激活。坏死被 caspase 独立的刽子手激活。在这研究,我们证明导致 apoptosis 调停蛋白质的坏死的 PCD 小径在 CHMP5 抑制以后被激活。autophagic PCD 没发生在 CHMP5 缺乏的 U937 房间,这被发现。在结论,在 CHMP5 抑制,两条 Granzyme B/Perforin apoptotic 小径和导致 apoptosis 以后,调停因素的坏死的小径被激活,当 autophagic 小径没被激活时。 | Hairong Wang Jia Liu Feilong Wang Miao Chen Zhenyu Xiao Renrong OuYang Aihua Fei Yanlin Shen Shuming Pan | 2013 | Acta Biochimica et Biophysica Sinica2013，45，5： | 3 |
| 2 | Interleukin-13 inhibits cytokines synthesis by blocking nuclear factor-κB and c-Jun N-terminal kinase in human mesangial cells显示文摘Objective:Monocytes/macrophages,proinflammatory cytokines and chemokines are important in the pathogenesis of glomerulonephritis.Interleukin(IL)-13 has been shown to exert potent anti-inflammatory properties. This study was designed to investigate the effect of IL-13 on the expression of proinflammatory cytokines,chemokines and profibrogenic cytokines and the involved molecular mechanism in cultured human mesangial cells (HMCs).Methods:The expressions of proinflammatory cytokines,chemokines and profibrogenic cytokines were determined by ribonuclease protection assay(RPA).Activity of nuclear factor-kappa B(NF-κB)and activa- tor protein-1(AP-1)was examined by electrophoretic mobility shift assay(EMSA).NF-κB subunit p65 nuclear transportation and c-Jun N-terminal kinase(JNK)activity were assayed by immunoblot.Results:Recombinant IL-13 inhibited tumor necrosis factor-α(TNF-α),IL-1α,IL-1β,monocyte chemoattractant protein-1(MCP-1), IL-8,and transforming growth factor-β1(TGF-β1)mRNA expressions in a dose-dependent manner.Lipopoly-sacchorides(LPS)dramatically increased NF-κB DNA binding activity of HMCs,which was inhibited by IL-13 in a dose-dependent manner.LPS-activated NF-κB contained p50 and p65 dimers,but not c-Rel subunit.IL-13 blocked LPS-induced NF-κB subunit p65.LPS stimulated JNK/AP-1 activation,which was inhibited by IL-13 in a dose-dependent manner.Conclusion:IL-13 inhibits proinflammatory cytokines,chemokines,and profibrogenic cytokines synthesis by blocking NF-κB and JNK/AP-1 activation.These observations point to the importance of IL-13 in the modulation of inflammatory processes in the renal glomerulus. | Chunhua Zhu Aihua Zhang Songming Huang GuixiaDing Xiaoqin Pan Ronghua Chen | 2010 | The Journal of Biomedical Research2010，24，4： | 2 |
| 3 | Potential chronic liver toxicity in rats orally administered an ethanol extract of Huangqin(Radix Scutellariae Baicalensis)显示文摘OBJECTIVE: To investigate the potential chronic liver toxicity of oral administration of ethanol extract of Huangqin(Radix Scutellariae Baicalensis)(SBE) in Wistar rats.METHODS: SBE was administered to rats by gavage for 26 weeks, at doses of 300, 1250, or 2500 mg·kg-1·d-1 respectively. The rats were euthanized at the end of 13 and 26 weeks daily oral dosing and following 4 weeks of recovery time. The changes of hematology, urinary, blood biochemistry and histomorphology were examined at each time point and focus on liver function and histological changes.RESULTS: When SBE at a dose of up to 2500 mgkg-1 d-1 was fed to male and female rats for 2··6 weeks, the liver tissue showed some inflammatory change that predominated by leukocyte infiltrationbut returned to normal after withdrawal. In addition, high-dose SBE treatment of 26 weeks in rats,glucose, electrolyte and lipid levels also have some changes. In addition, there are no other functional or organic lesions related to SBE treatment.CONCLUSIONS: Long-term and high-dose SBE may cause liver damage, however, the structural damage of the liver can be restored after the ethanol extract stopping. SBE will be well-tolerated for long-term use as a drug or health food, but in order to ensure drug safety, liver function, and serum glucose, electrolyte and lipid levels should be monitored when using SBE long term. | Yi Yan Zhao Yong Li Chunying Zhang Yushi Bin Yang Yuan Yalan Pan Chen Wang Lianmei Liang Aihua | 2018 | Journal of Traditional Chinese Medicine2018，38，2： | 1 |
| 4 | Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon 1445 and Mon 531显示文摘 | YANG Litao PAN Aihua ZHANG Kewei | 2005 | Transgenic Research2005，14，6： | 1 |
| 5 | Event-specific qualitative and quantitative PCR detection of genetically modified MON863 maize based on the 5'-transgene integration sequence显示文摘 | YANG Litao XU Songci PAN Aihua | 2005 | Journal of Agricultural and Food Chemistry2005，53，24： | 1 |
| 6 | Event-specific qualitative and quantitative PCR detection of MON863 maize based upon the 3'-Wansgene integration sequence显示文摘 | PAN Aihua YANG Litao XU Songci | 2006 | Journal of Cereal Science2006，43，2： | 1 |
| 7 | Effects of static magnetic fields on Escherichia coli显示文摘 | Wenjin Ji Huimin Huang Aihua Deng Chunyang Pan | 2009 | Micron2009，，8： | 1 |
| 8 | In vitro assembly of the bacterial actin protein MamK from 'Candidatus Magnetobacterium casensis' in the phylum Nitrospirae显示文摘 | Aihua Deng Wei Lin Nana Shi Jie Wu Zhaopeng Sun Qinyun Sun Hua Bai Yongxin Pan Tingyi Wen | 2016 | Protein & Cell2016，7，4： | 1 |
| 9 | Expression of mouse metallothionein-I gene confers cadmium resistance in transgenic tobacco plants显示文摘 | Aihua Pan Meizhu Yang Feng Tie Lingyua Li Zhangliang Chen Biggen Ru | 1994 | Plant Molecular Biology1994，，2： | 1 |
| 10 | Superb VOCs capture engineering carbon adsorbent derived from shaddock peel owning uncompromising thermal-stability and adsorption property显示文摘High applied thermal-stability and superior structural property for activated carbon adsorbent are extremely promising,which also is the determining short slab in volatile organic compounds(VOCs)adsorption applications.Herein,we develop the outstanding engineering carbon adsorbents from waste shaddock peel which affords greatly-enhanced thermal-stability and super structural property(S_(Lang)=4962.6 m2·g^(-1),Vmicro=1.67 cm^(3)·g^(-1)).Such character endows the obtained adsorbent with ultrahigh adsorption capture performance of VOCs specific to benzene(16.58 mmol·g^(-1))and toluene(15.50 mmol·g^(-1),far beyond traditional zeolite and activated carbon even MOFs materials.The structural expression characters were accurately correlated with excellent adsorption efficiency of VOCs by studying synthetic factor-controlling comparative samples.Ulteriorly,adsorption selectivity prediction at different relative humidity was demonstrated through DIH(difference of the isosteric heats),exceedingly highlighting great superiority(nearly sixfold)in selective adsorption of toluene compared to volatile benzene.Our findings provide the possibility for practical industrial application and fabrication of waste biomass-derived outstanding biochar adsorbent in the environmental treatment of threatening VOCs pollutants. | Fu Yang Wenhao Li Rui Ou Yutong Lu Xuexue Dong Wenlong Tu Wenjian Zhu Xuyu Wang Lulu Li Aihua Yuan Jianming Pan | 2022 | Chinese Journal of Chemical Engineering2022，35，7： | 1 |
| 11 | Mesoporous magnetic nanoparticles conjugated aptamers for exosomes capture and detection of Alzheimer’s disease显示文摘Exosomes are nanoscale membrane-enclosed extracellular vesicles secreted by various cells,which have enormous potential as disease biomarkers for clinical application.However,the isolation and detection of exosomes remain enormous challenges,which limits their further application.Herein,inspired by immunomagnetic beads,a magnetic nanoparticle conjugated aptamer was repurposed for the effective capture and detection of exosomes.The magnetic nanoparticles,composed of Fe 3 O 4 synthesized by the hydrothermal method as the core and coupled with gold nanoparticles(Fe 3 O 4@Au),provide a large specific surface area,making the resulting composite material an effective platform for exosome capture.Furthermore,the elution of captured exosomes with 1.0 M NaCl made downstream analysis of exosomes possible.The preliminary clinical application value of the composite in exosome analyses of serum from healthy individuals and patients with Alzheimer’s disease(AD)has also been verified,which could provide a promising platform for biomedical and clinical diagnosis. | Guidan Wang Zhenshan Pan Xiaorui Zhu Ruyue Yang Rong Yang Tingting Yang Dong Hu Aihua Jing Gaofeng Liang | 2023 | Engineered Regeneration2023，4，4： | 0 |
| 12 | Modeling and experiments on Galfenol energy harvester显示文摘Vibration energy harvesting can solve the energy supplying problem of systems like wireless sensors networks.The Galfenol cantilever beam energy harvester is suitable for this application.According to the electromechanical conversion principle,the constitutive relation of Galfenol is built.A magnetization model is also established,based on the hysteresis model of Galfenol.Combining the magneto-mechanical coupling model,the constitutive relation of Galfenol and the electromagnetic induction law,the mathematical model of Galfenol vibration energy harvester is established.A hyperbolic curve-shaped cantilever beam is designed and its performance is compared to three types of cantilever beams:rectangle,trapezoidal,and triangle.The stress distribution,modal analysis and frequency response of these four shapes of beams are compared.The hyperbolic beam is more suitable for low frequency vibration harvesting.The strain on the beams,output voltage and power output response to these energy harvesters,under different natural vibration frequencies,are determined by simulation.Finally,the simulation results are compared to experimental electric outputs of all four types of prototype beams.The comparative study showed consistency between the experimental and the simulation results,and also that the peak-to-peak induction voltage value of the hyperbolic beam is larger than other shapes of energy harvesters,whose average value is at 269.8 mV.The maximum power output of the hyperbolic beam is 403.8μW when connected with a 50Ω resistance. | Aihua Meng Chun Yan Mingfan Li Wenwu Pan Jianfeng Yang Shuaibing Wu | 2020 | Acta Mechanica Sinica2020，36，3： | 0 |
| 13 | Toxicity study of ethanol extract from Oroxylumindicum(L.) Vent in rats显示文摘OBJECTIVE: To evaluate the safety of Oroxylumindicum(L.) Vent extract administered for 26 weeks in Wistar rats.METHODS: Oroxylumindicum(L.) Vent extract was administrated to male and female rats by gavage once daily at doses of 54, 225, and450 mg·kg-1·d-1.The rats were sacrificed after administration for13 weeks and 26 weeks. Part of the rats in each group were allowed to recover for 4 weeks after 26-week administration. Systematic examinations including haematology, urology, blood biochemistry and histomorphology were performed at the end of13, 26 weeks of administration and 4 weeks of recovery.RESULTS: No treatment related adverse effect shappened on rats' general status, body weight, food consumption, urinary index and histomorphologyexamination. Although during the administration,in some rats of extract's groups, the value of Red blood cell count(RBC), white blood cell counts(WBC), hemoglobin(HGB), haematocrit(HCT) and K iron were decreased, and biochemistry index,such as glucose(GLU), triglyceride(TG), alanine transaminase(ALT) and blood urea nitrogen(BUN)were increased, the above parameters were within the normal ranges and all returned to baseline after the drug stopping for 4 weeks.CONCLUSION: The administration of Oroxylumindicum(L.) Vent extractat levers up to 450 mg/kg(equals to 75 times of clinical dose) is well toleratedfor both genders without significant toxicity within the administration duration in this study. | Yi Yan Zhao Yong Li Chunying Zhang Yushi Bin Yang Yuan Yalan Wang Lianmei Pan Chen Liang Aihua | 2018 | Journal of Traditional Chinese Medicine2018，38，5： | 0 |
| 14 | Deposition Effects of Electrostatic and NonElectrostatic Aerial Spray显示文摘In this study,we compared the aerial electrostatic spray with non-electrostatic spray by helicopter R44 and fixed-wing aircraft Y5B equipped with an own-made aerial electrostatic spraying system.The results showed that the spray deposits on targets and leaves of tree crowns were notable enhanced comparing with the non-electrostatic and conventional aerial spray.The spray deposits were fine,uniform and the densities were 1.0-1.75 times higher than the non-electrostatic spray.Deposit rate of the electrostatic spray on the backs of leaves by helicopter R44 was 2.15 times of the non-electrostatic, and 3.31 times of the conventional aerial spray.The deposit rate of the electrostatic spray on the backs of leaves by the Y5B was 2.38 times of the non-electrostatic,and 3.44 times of the conventional aerial spray. | Shu Chaoran Pan Hongyang Zhou Hongping Zhan Min Mei Aihua Ru Yu Jia Zhicheng Chen Guofa | 2012 | Chinese Forestry Science and Technology2012，11，3： | 0 |
| 15 | SorCS2 neurofibrillary tangles-like lesions in AD human cerebrum显示文摘Recent findings have indicated that SorCS2,a member of the vacuolar protein sorting 10 protein(VPS10P)family,has relevance to bipolar disorder,schizophrenia,and Alzheimer's disease(AD).We observed SorCS2 can accumulate as neurofibrillary tangles(NFTs)-like lesions in AD human brain cerebrum.Then we set to explore NFTs-like lesions from SorCS2 in aged and AD human cerebrum,and whether this pathological hallmark is unique to AD human brains.We characterized SorCS2 changes in postmortem human brains(n=10)from aged and AD subjects. | Hu Dandan Li Yanan Tu Tian Jiang Juan Yan Xiaoxin Pan Aihua | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 16 | Characterization of gradient Ni-Fe/SiC composite coating on mild steel by thermal spraying in combination with laser cladding显示文摘Metal matrix composite coating Ni-Fe/SiC was prepared on an iron-based substrate bythermal spraying combined with laser cladding,using SiC particulates as the reinforcing agent.The micro-structures of the coatings formed at different thermal spraying and laser cladding conditions were character-ized by means of X-ray diffraction and electron probe microanalysis.The thermal oxidation properties of themixed powders composed of different content of SiC particulates and relevant Ni-based alloy as the balancewere examined using differential scanning calorimetry.The hardness profile of the thermal sprayed and lasercladding coatings was investigated as well.It was found that SiO2particulates were generated and dissolvedand dispersed during the melting and solidification of the laser cladding process,which was ascribed to theoxidation of the dispersed SiC particulates.The micro-hardness depth profile of the target laser claddingcomposite coating was characterized by gradient distribution,which could be related to the gradient distribu-tion of the hard SiC and SiO2particulates in the dendrites and interdendrites of the cladding layer.Both SiCand SiO2particulates contributed to greatly increasing the microhardness and mechanical properties of the ti-tled laser cladding composite coatings. | Hu Mulin Pan Lin Fu Yongqing Xie Changsheng Wang Aihua | 2005 | 材料保护2005，38，11： | 0 |
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