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| 1 | Virus-induced Phytoene Desaturase(PDS) Gene Silencing Using Tobacco Rattle Virus in Lilium × formolongi显示文摘Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing(VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco rattle virus(TRV)-based VIGS of a PHYTOENE DESATURASE(PDS) ortholog(LhPDS) can be achieved in Lilium × formolongi seedlings, with a survival rate of 92%, using the inoculation method of rubbing plus injection. Compared with untreated and mock-treated seedlings, the photobleached leaf phenotype of silenced plants significantly correlates with down-regulation of endogenous LhPDS(P ≤ 0.05). In addition, the silencing phenomenon can be observed in the growing points of plants, indicating that systemic viral infection was achieved using the protocol. The results indicate that TRV-based VIGS can be used to characterize gene function in Lilium × formolongi. This work lays the foundation for gene function analysis and molecular breeding in Lilium spp. | Hua Xu Leifeng Xu Panpan Yang Yuwei Cao Yuchao Tang Guoren He Suxia Yuan Jingyi Lei Jun Ming | 2019 | Horticultural Plant Journal2019，5，1： | 6 |
| 2 | Rechargeable quasi-solid-state aqueous hybrid Al^(3+)/H^(+) battery with 10,000 ultralong cycle stability and smart switching capability显示文摘Safe and long lifespan batteries facilitate the development of portable electronics and electric vehicles.Owing to the low-cost,naturally abundance,and trivalent charge carrier of aluminum with the highest theoretical volumetric capacity,rechargeable aqueous aluminum-ion-based batteries are considered as promising next-generation secondary batteries.However,traditional electrolytes and frequent collapse of the host structure of electrode materials greatly jeopardize the cycle stability of the batteries.Here,we develop a novel hydrogel-based electrolyte coupled with stable layered intercalation electrodes for the first time to fabricate a highly safe and flexible rechargeable hybrid Al^(3^(+))/H^(+)battery.The as-fabricated hybrid-ion battery(HIB)delivers a high specific capacity of 125 mAh·g^(−1) at 0.1 A·g^(−1) and exhibits an unprecedented super long-term cycling stability with no capacity fading over 10,000 cycles at 2 A·g^(−1).In addition,the hydrogel-based electrolyte possesses smart function of thermoresponsive switching,which can effectively prevent thermal runaway for the batteries.The unprecedented long cycle stability,highly intrinsic safety as well as low-cost indicate that the flexible aqueous HIBs are promising for applications. | Hua Wang Panpan Wang Zhenyuan Ji Zhe Chen Jiaqi Wang Wei Ling Jie Liu Mengmeng Hu Chunyi Zhi Yan Huang | 2021 | Nano Research2021，14，11： | 1 |
| 3 | Tailoring grain morphology in Ti-6Al-3Mo through heterogeneous nucleation in directed energy deposition显示文摘A common challenge in direct energy deposition(DED)is eliminating the anisotropy in mechanical performance associated with microstructure and the formation of coarse columnar grains.In this work,a heterogeneous nucleation mechanism was introduced into the melt pool,and,from this mechanism,an almost fully equiaxed grain morphology was obtained in the DED of Ti-6 Al-3 Mo.Three types of grain morphologies in DED Ti-6 Al-3 Mo,including full columnar grains,near-equiaxed grains and almost fully equiaxed grains were obtained from premixed and satellite powder blends from Ti,6 wt.%Al and 3 wt.%Mo,respectively.Combined with the analysis of the interactions between powder particles and the melt pool in DED,the formation mechanism of the equiaxed grains caused by the incomplete melting of high melting point Mo particles was revealed.As the prior-βgrains transformed from coarse columnar grains to fine-equiaxed grains,the strong<100>fiber texture along the deposition direction was weakened,while the size of theα-laths in the prior-βgrains slightly decreased,and the selection ofα-variants was weakened.Due to the transformation of the prior-βgrains from coarse columnar grains to fine-equiaxed grains,the tensile strength of the deposited samples increased from 982 MPa to 1082 MPa,while the yield strength increased from 840 MPa to 922 MPa,and the elongation of the as-deposited alloy also increased from 9.0%to 9.8%,which confirmed that the presence of fine-equiaxed grains is beneficial to the strength and plasticity of the DED alloy.This work further demonstrates the role that satelliting powders can play in terms of enhancing the columnar to equiaxed transition(CET)behavior associated with DED. | Fengying Zhang Panpan Gao Hua Tan Yao Li Yongnan Chen Min Mei Adam T.Clare Lai-Chang Zhang | 2021 | Journal of Materials Science & Technology2021，，29： | 1 |
| 4 | SrAl_(2)O_(4) crystallite embedded inorganic medium with super-long persistent luminescence,thermoluminescence, and photostimulable luminescence for smart optical information storage显示文摘As a leader of long persistent luminescence(LPL) materials, the optical properties of aluminate phosphor have remained unsurpassed for many years. As a powder material, its practical application will always be limited to the field of security signs. In this paper, the SrAl_(2)O_(4)∶Eu^(2+), Dy^(3+)inorganic solid material with comparable LPL properties to powder materials was obtained. The crystallization mechanism and crystallite micro-morphology of inorganic glass materials have been studied, and a new opinion is put forward that the large-size SrAl_(2)O_(4)crystallites in the glass matrix are stacked by rod-shaped crystals arranged in a regular direction. In addition,the SrAl_(2)O_(4)∶Eu^(2+), Dy^(3+)glass obtained cannot only collect high-energy photons but also is sensitive to lowenergy sunlight. The results show that the material exhibits superior performance in LPL, thermoluminescence,and photostimulable luminescence. Based on this property, a new application of this material in the field of information storage was explored. This paper has a certain reference value for the development and application of aluminate LPL materials in the field of smart optical information storage. | Panpan Li Youjie Hua Renguang Ye Muzhi Cai Shiqing Xu Junjie Zhang | 2022 | Photonics Research2022，10，2： | 1 |
| 5 | High?Voltage Flexible Aqueous Zn?Ion Battery with Extremely Low Dropout Voltage and Super?Flat Platform显示文摘Flexible rechargeable aqueous zinc-ion batteries(ZIBs)have attracted extensive attentions in the energy storage field due to their high safety,environmental friendliness,and outstanding electrochemical performance while the exploration of high-voltage aqueous ZIBs with excellent rate capability is still a great challenge for the further application them in flexible and wearable electronics.Herein,we fabricated a 2.4 V high-voltage flexible aqueous ZIB,being among the highest voltage reported in aqueous ZIBs.Moreover,it exhibits extremely flat charging/discharging voltage platforms and the dropout voltage is only 0.1 V,which is the smallest gap in all aqueous batteries to our best knowledge.Furthermore,the prepared ZIB performs high rate capability of 25 C and energy density of 120 Wh kg?1 and exhibits excellent safety under various destructive conditions including hammering,sewing,punching,and soaking.These extraordinary results indicate the great application potential of our high-voltage flexible aqueous ZIB in wearable electronics. | Zhe Chen Panpan Wang Zhenyuan Ji Hua Wang Jie Liu Jiaqi Wang Mengmeng Hu Yan Huang | 2020 | Nano-Micro Letters2020，12，6： | 0 |
| 6 | Flexible aqueous Ca-ion full battery with super-flat discharge voltage plateau显示文摘Recently,multivalent metal-ion batteries have attracted considerable interests on the merits of their natural abundance and multielectron redox property.However,the development of Ca-ion battery is still in their preliminary stage because of the lack of suitable electrode material.The Ca-storage performance of the existing materials is still unsatisfactory with low capacity,poor cyclic stability,as well as sloping discharge profiles,which cannot provide stable energy output.In this work,transition metal oxide Sn-doped In2O3(ITO)has been explored as the aqueous Ca-ion battery anode,which could deliver a high discharge capacity of 71.2 mAh·g^(-1) with an ultra-flat discharge voltage plateau.The Ca storage mechanism was revealed to be reversible conversion reaction based on ex-situ X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),and transmission electron microscopy(TEM)characterizations.A flexible aqueous Ca-ion battery was subsequently assembled with zinc hexacyanoferrate(ZnHCF)cathode and ITO anode sandwiched by hydrogel electrolyte,which could deliver a high specific capacity of 75.3 mAh·g^(-1) at 0.4 A·g^(-1) with a flat output voltage plateau at around 0.8 V.The bendable and flexible Ca-ion battery with decent voltage output will pave the way for the energy storage devices towards practical applications in flexible and wearable electronics. | Panpan Wang Hua Wang Zhe Chen Junwei Wu Jingting Luo Yan Huang | 2022 | Nano Research2022，15，1： | 0 |
| 7 | Successive digestion of tilapia collagen by serine proteinase and proline specific endopeptidase to produce novel angiotensin l-converting enzyme inhibitory peptides显示文摘Serine proteinase,purified from the hepatopancreas of Pacific white shrimp(Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen(ASC)isolated from Nile tilapia(Oreochromis sp.)skin to produce angiotensin I-converting enzyme(ACE)inhibitory peptides(ACEIPs).A series of column chromatography assays were used to separate the ACEIPs.A peptide,NPARTCR,was isolated as it exhibited high ACE inhibition potential.Further digestion of this peptide by a proline specific endopeptidase(PSEP),produced a pentapeptide ARTCR with ACE inhibitory activity(IC_(50))of 77.0 pmol/L.Both NPARTCR and ARTCR inhibited ACE in a non-competitive manner.An in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats(SHRs).These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen,respectively.Serine proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension. | Xin Hua Lechang Sun Chan Zhong Qiang Wu Panpan Xiao Asami Yoshida Guangming Liu Minjie Cao | 2020 | Marine Life Science & Technology2020，2，3： | 0 |
| 8 | Effect of Microstructural Characteristics on Fracture Toughness in Direct Energy Deposited Novel Ti-6Al-4V-1Mo Alloy显示文摘Meeting the damage tolerance requirements for engineering-grade titanium alloys pose a significant challenge in achieving high fracture toughness in direct energy deposition(DED)titanium alloys.This work primarily investigated the relationship between the microstructure and the fracture toughness of DED new Ti-6Al-4V-1Mo alloy.Two types of microstructures were designed via two process strategies:high-line energy density(HE)and low-line energy density(LE).Relative to LE samples,HE samples possess larger-sized microstructural characteristics(coarser grain boundaryα(α_(GB)),largerαcolonies,and coarserαlaths).Lessα/βphase boundaries were formed by coarserαlaths in the HE samples,increasing the movement of dislocations,resulting in tensile strength decreasing from 1007.1 MPa(LE)to 930.8 MPa(HE)and elongation increasing from 10.8%(LE)to 15.7%(HE).Also,HE samples exhibited an excellent fracture toughness of 114.0 MPa m^(1/2),significantly higher than that of LE samples(76.8 MPa m^(1/2)).An analysis of crack propagation paths was conducted to investigate the factors contributing to toughening.The primary factor enhancing toughness is the frequent obstruction of cracks by coarseαGB and largeαcolonies in HE samples.Particularly,the pretty large-angle deflections induced by the superposition effect of coarseαGB and largeαcolonies play a vital of significant role.These factors induced the long and tortuous high-energy pathways,which resulted in ultimately improved fracture toughness.The discovered microstructural toughening mechanisms can serve as a reference for future studies involving titanium alloys,offering insights on how to enhance fracture toughness by achieving similar characteristics. | Chao Xia Kexin Zhao Xin Zhou Yuqi He Panpan Gao Hengxin Zhang Guangrui Gao Fengying Zhang Hua Tan | 2024 | Acta Metallurgica Sinica(English Letters)2024，37，1： | 0 |
| 9 | Genomic dissection of gastrointestinal and lung neuroendocrine neoplasm显示文摘Objective:Neuroendocrine neoplasms(NENs)are relatively rare and heterogeneous malignancies with two major subtypes:low-grade neuroendocrine tumor(NET)and high-grade neuroendocrine carcinoma(NEC).Comprehensive molecular characterization of NENs is needed to refine our understanding of the biological underpinnings of different NEN subtypes and to predict disease progression more accurately.Methods:We performed whole-exome sequencing(WES)of NEN samples from 49 patients(25 NETs and 24 NECs)arising from the stomach,intestines or lung.Clinicopathologic features were assessed and associated with molecular events.Results:NENs generally harbor a low mutation burden,with TP53 being the top mutated gene found in 31%of patients.Consistent with other studies,p53 signaling pathway dysfunction is significantly enriched in NECs compared to NETs(P<0.01).Other than TP53,tissue type-specific mutation profiles of NENs were observed in our cohort compared to those reported in pancreatic NETs.Importantly,we observed significant genomic instability,with increased copy number alterations observed across the NEN genome,which was more profound in NECs and independently correlated with poor overall survival(OS)(P<0.001).NECs could be further stratified into two molecular subtypes based on OS(P<0.001)and the chromosomal instability score(CIS).Interestingly,we discovered that the gain of whole chromosome 5 occurred at the early stage of NEN development,followed by the loss of 5 q exclusively in NECs(P<0.001).Conclusions:These findings provide novel insights into the molecular characteristics of NENs and highlight the association of genomic stability with clinical outcomes. | Haixing Wang Li Sun Hua Bao Ao Wang Panpan Zhang Xue Wu Xiaoling Tong Xiaonan Wang Jie Luo Lin Shen Yang WShao Ming Lu | 2019 | Chinese Journal of Cancer Research2019，31，6： | 0 |
| 10 | High-contrast flicker luminescence on dynamic covalent structure based nanoaggregates显示文摘In nanoscience, molecular switches have played a significant role to deliver different control abilities to practical functions,whereas high-contrast luminescence switchable manipulation at nanoscale is still limited. Since the tuning for emission behavior with high contrast ratio strongly connects to numerous visualized sensing and optoelectronic applications, we here report that autonomous p H control can be enrolled to address a high-contrast molecular emission change at the nanoaggregated level, for gaining a flicker luminescence performance in aqueous media. Employing a BODIPY contained dynamic covalent dye, we find its luminescent signal and nanoaggregate size can be spontaneously adjusted in water. On this basis, high-contrast luminescence switching of the material can be achieved upon the alternate introduction of base and acid into the aggregation state. Such a behavior can be attributed to a p H triggered photo-induced electron transfer regulation process. The dye aggregates can be well endocytosed for bioimaging and its luminescent variation can be autonomously displayed as a flicker effect. These results provide new visions for the design and development of smart materials with a dynamic luminescence behavior. | Yunyun Zhou Panpan Hua Bin Wu Xiaoyan Bao Xuping Li Liangliang Zhu | 2019 | Science China Chemistry2019，62，2： | 0 |
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