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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Protective effects of resveratrol on postmenopausal osteoporosis: regulation of SIRT1-NF-κB signaling pathway显示文摘绝经后的骨质疏松症严重地使受危险人的健康。没有副作用,寻找治疗学的药必要性大。我们的学习被设计调查是否 resveratrol, SIRT1 的收缩筋,能在骨质疏松症上并且到有有利效果探索内在的机制。老鼠骨质疏松症模型(卵巢切除术组, OVX ) 被双边的卵巢切除术建立。resveratrol 的三不同剂量被使用:5 mg/kg/d (低剂量,物件 LD ), 25 mg/kg/d (开媒介,物件 MD ), 和 45 mg/kg/d (开高度,物件 HD ) 。结果显示出那物件 LD 没在 OVX 改变上显示出任何重要效果,当时物件 MD 和物件 HD 显著地提高了骨质疏松症导致的减少的骨头矿物质密度(物件 MD 0.205 吗? | Jing Feng Shuai Liu Sai Ma Jian Zhao Wei Zhang Wei Qi Pengchong Cao Zheng Wang Wei Lei | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 70 |
| 3 | The Genome of Dendrobium officinale Illuminates the Biology of the Important Traditional Chinese Orchid Herb显示文摘 | Liang Yan Xiao Wang Hui Liu Yang Tian Jinmin Lian Ruijuan Yang ShumeiHao Xuanjun Wang Shengchao Yang Qiye Li Shuai Qi Ling Kui Moses Okpekum Xiao Ma Jiajin Zhang Zhaoli Ding Guojie Zhang Wen Wang Yang Dong Jun Sheng | 2015 | Molecular Plant2015，8，6： | 64 |
| 4 | Effects of different resuscitation fluid on severe acute pancreatitis显示文摘AIM: To compare effects of different resuscitation fluid on microcirculation, inflammation, intestinal barrier and clinical results in severe acute pancreatitis (SAP). METHODS: One hundred and twenty patients with SAP were enrolled at the Pancreatic Disease Institute between January 2007 and March 2010. The patients were randomly treated with normal saline (NS group), combination of normal saline and hydroxyethyl starch (HES) (SH group), combination of normal saline, hydroxyethyl starch and glutamine (SHG group) in resuscitation. The ratio of normal saline to HES in the SH and SHG groups was 3:1. The glutamine (20% glutamine dipeptide, 100 mL/d) was supplemented into the resuscitation liquid in the SHG group. Complications and outcomes including respiratory and abdominal infection, sepsis, abdominal hemorrhage, intra-abdominal hypertension, abdominal compartment syndrome (ACS), renal failure, acute respiratory distress syndrome (ARDS), multiple organ dysfunction syndrome (MODS), operation intervention, length of intensive care unit stay, length of hospital stay, and mortality at 60 d were compared. Moreover, blood oxygen saturation (SpO 2 ), gastric intramucosal pH value (pHi), intra-abdominal pressure (IAP), inflammation cytokines, urine lactulose/mannitol (L/M) ratio, and serum endotoxin were investigated to evaluate the inflammatory reaction and gut barrier. RESULTS: Compared to the NS group, patients in the SH and SHG groups accessed the endpoint more quickly (3.9 ± 0.23 d and 4.1 ± 0.21 d vs 5.8 ± 0.25 d, P < 0.05) with less fluid volume (67.26 ± 28.53 mL/kg/d, 61.79 ± 27.61 mL/kg per day vs 85.23 ± 21.27 mL/kg per day, P < 0.05). Compared to the NS group, incidence of renal dysfunction, ARDS, MODS and ACS in the SH and SHG groups was obviously lower. Furthermore, incidence of respiratory and abdominal infection was significantly decreased in the SH and SHG groups, while no significant difference in sepsis was seen. Moreover, less operation time was needed in the SH and SHG group than the NS group, but the difference was not significant. The mortality did not differ significantly among these groups. Blood SpO 2 and gastric mucosal pHi in the SH and SHG groups increased more quickly than in the NS group, while IAP was significantly decreased in the SH and SHG group. Moreover, the serum tumor necrosis factor-α, interleukin-8 and C-reactive protein levels in the SH and SHG groups were obviously lower than in the NS group at each time point. Furthermore, urine L/M ratio and serum endotoxin were significantly lower in the SH group and further decreased in the SHG group.CONCLUSION: Results indicated that combination of normal saline, HES and glutamine are more efficient in resuscitation of SAP by relieving inflammation and sustaining the intestinal barrier. | Gang Zhao Jun-Gang Zhang He-Shui Wu Jin Tao Qi Qin Shi-Chang Deng Yang Liu Lin Liu Bo Wang Kui Tian Xiang Li Shuai Zhu Chun-You Wang | 2013 | World Journal of Gastroenterology2013，19，13： | 56 |
| 5 | Regionalization based on spatial and seasonal variation in ground-level ozone concentrations across China显示文摘Owing to the vast territory of China and strong regional characteristic of ozone pollution,it's desirable for policy makers to have a targeted and prioritized regulation and ozone pollution control strategy in China based on scientific evidences. It's important to assess its current pollution status as well as spatial and temporal variation patterns across China.Recent advances of national monitoring networks provide an opportunity to insight the actions of ozone pollution. Here, we present rotated empirical orthogonal function(REOF)analysis that was used on studying the spatiotemporal characteristics of daily ozone concentrations. Based on results of REOF analysis in pollution seasons for 3 years' observations, twelve regions with clear patterns were identified in China. The patterns of temporal variation of ozone in each region were separated well and different from each other, reflecting local meteorological, photochemical or pollution features. A rising trend in annual averaged Eight-hour Average Ozone Concentrations(O_3-8 hr) from 2014 to 2016 was observed for all regions, except for the Tibetan Plateau. The mean values of annual and 90 percentile concentrations for all 338 cities were 82.6 ± 14.6 and 133.9 ± 25.8 μg/m^3,respectively, in 2015. The regionalization results of ozone were found to be influenced greatly by terrain features, indicating significant terrain and landform effects on ozone spatial correlations. Among 12 regions, North China Plain, Huanghuai Plain, Central Yangtze River Plain, Pearl River Delta and Sichuan Basin were realized as priority regions for mitigation strategies, due to their higher ozone concentrations and dense population. | Linjun Cheng Shuai Wang Zhengyu Gong Hong Li Qi Yang Yeyao Wang | 2018 | Journal of Environmental Sciences2018，30，5： | 14 |
| 6 | Limb remote ischemic postconditioning protects integrity of the blood-brain barrier after stroke显示文摘Integrity of the blood-brain barrier structure is essential for maintaining the internal environment of the brain.Development of cerebral infarction and brain edema is strongly associated with blood-brain barrier leakage.Therefore,studies have suggested that protecting the blood-brain barrier may be an effective method for treating acute stroke.To examine this possibility,stroke model rats were established by middle cerebral artery occlusion and reperfusion.Remote ischemic postconditioning was immediately induced by three cycles of 10-minute ischemia/10-minute reperfusion of bilateral hind limbs at the beginning of middle cerebral artery occlusion reperfusion.Neurological function of rat models was evaluated using Zea Longa's method.Permeability of the blood-brain barrier was assessed by Evans blue leakage.Infarct volume and brain edema were evaluated using 2,3,5-triphenyltetrazolium chloride staining.Expression of matrix metalloproteinase-9 and claudin-5 m RNA was determined by real-time quantitative reverse transcription-polymerase chain reaction.Expression of matrix metalloproteinase-9 and claudin-5 protein was measured by western blot assay.The number of matrix metalloproteinase-9-and claudin-5-positive cells was analyzed using immunohistochemistry.Our results showed that remote ischemic postconditioning alleviated disruption of the blood-brain barrier,reduced infarct volume and edema,decreased expression of matrix metalloproteinase-9 m RNA and protein and the number of positive cells,increased expression of claudin-5 m RNA and protein and the number of positive cells,and remarkably improved neurological function.These findings confirm that by suppressing expression of matrix metalloproteinase-9 and claudin-5 induced by acute ischemia/reperfusion,remote ischemic postconditioning reduces blood-brain barrier injury,mitigates ischemic injury,and exerts protective effects on the brain. | Juan Li Xiao-Song Hu Fang-Fang Zhou Shuai Li You-Sheng Lin Wen-Qian Qi Cun-Fang Qi Xiao Zhang | 2018 | Neural Regeneration Research2018，13，9： | 11 |
| 7 | Single-cell transcriptomic atlas of primate cardiopulmonary aging显示文摘Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019(COVID-19).Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed.Here,we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung,heart and artery,the top tissues targeted by SARS-CoV-2.Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging.With age,expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2(ACE2)was increased in the pulmonary alveolar epithelial barrier,cardiomyocytes,and vascular endothelial cells.We found that interleukin 7(IL7)accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner.Furthermore,treatment with vitamin C blocked IL7-induced ACE2 expression.Altogether,our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2,suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly. | Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu | 2021 | Cell Research2021，31，4： | 10 |
| 8 | Petrogenesis, Tectonic Evolution and Geothermal Implications of Mesozoic Granites in the Huangshadong Geothermal Field, South China显示文摘Mesozoic multi-stage tectono-magmatic events produced widely distributed granitoids in the South China Block. Huangshadong(HSD) is located in south-eastern South China Block, where closely spaced hot springs accompany outcrops of Mesozoic granites. New data on whole-rock geochemistry, zircon U-Pb geochronology, and zircon Lu-Hf isotopes are presented, to study the petrogenesis and tectonic evolution of the granites, and to explore the relationship between granites and geothermal anomalies. Zircon U-Pb isotopes display three periods of granites in the HSD area: Indosinian(ca. 253 Ma, G4) muscovite-bearing monzonitic granite, early Yanshanian(ca. 175–155 Ma, G5 and G3) monzonitic granite and granodiorite, and late Yanshanian(ca. 140 Ma, G1 and G2) biotite monzonitic granite. In petrogenetic type, granites of the three periods are I-type granite. Among them, G1, G2, G3, and G4 are characterized by high fractionation, with high values of SiO2, alkalis, Ga/Al, and Rb/Sr, and depletion in Sr, Ba, Zr, Nb, Ti, REEs, with low(La/Yb)N, Nb/Ta, and Zr/Hf ratios and negative Eu anomalies. In terms of tectonic setting, 253 Ma G4 may be the product of partial melting of the ancient lower crust under post-orogenic extensional tectonics, as the closure of the Paleo-Tethys Ocean resulted in an intracontinental orogeny. At 175 Ma, the subduction of the Pacific Plate became the dominant tectonic system, and low-angle subduction of the Paleo-Pacific Plate facilitated partial melting of the subducted oceanic crust and basement to generate the hornblende-bearing I-type granodiorite. As the dip angle of the subducting plate increased, the continental arc tectonic setting was transformed to back-arc extension, inducing intense partial melting of the lower crust at ca. 158 Ma and resulting in the most frequent granitic magmatic activity in the South China hinterland. When slab foundering occurred at ca. 140 Ma, underplating of mantle-derived magmas caused melting of the continental crust, generating extensive highly fractionated granites in HSD. Combining the granitic evolution of HSD and adjacent areas and radioactive heat production rates, it is suggested that highly fractionated granites are connected to the enrichments in U and Th with magma evolution. The high radioactive heat derived from the Yanshanian granites is an important part of the crustal heat, which contributes significantly to the terrestrial heat flow. Drilling ZK8 reveals deep, ca. 140 Ma granite, which implies the heat source of the geothermal anomalies is mainly the concealed Yanshanian granites, combining the granite distribution on the surface. | Zhicai Xiao Shuai Wang Shihua Qi Jian Kuang Min Zhang Feng Tian Yongjie Han | 2020 | Journal of Earth Science2020，31，1： | 9 |
| 9 | Detection of serum IgM and IgG for COVID-19 diagnosis显示文摘Dear Editor,Infection with the novel coronavirus(SARS-CoV-2,which is the virus responsible for the coronavirus disease 2019(COVID-19))was first reported in Wuhan,China on December 31,2019.The outbreak of COVID-19 remains ongoing and was linked to more than 80,000 infected patients and more than 3,000 deaths in China as of March 7,2020(Holshue et al.,2020). | Ling Zhong Junlan Chuan Bo Gong Ping Shuai Yu Zhou Yi Zhang Zhilin Jiang Dingding Zhang Xiaoqi Liu Shi Ma Yi Huang He Lin Qingwei Wang Lulin Huang Dan Jiang Fang Hao Juan Tang Chunqi Zheng Hua Yu Zhibin Wang Qi Jiang Tao Zeng Mei Luo Fanwei Zeng Fanxin Zeng Jianghai Liu Junxi Tian Yu Xu Tengxiang Long Kaiju Xu Xingxiang Yang Yuping Liu Yi Shi Li Jiang Zhenglin Yang | 2020 | Science China(Life Sciences)2020，63，5： | 8 |
| 10 | Recent Progress in Automotive Gasoline Direct Injection Engine Technology显示文摘Gasoline direct injection(GDI)engines are currently the dominant powertrains for passenger cars.With the implementation of increasingly stringent fuel consumption and emission regulationsworldwide,GDI engines are facing challenges owing to high particulate matter emissions and a tendency to knock,leading to a change in the research and design(R&D)issues compared with those in the twentieth century.This paper reviews the progress in research regarding GDI engine technologies over the past 20 years,focusing on combustion system configurations,and also highlights common issues in GDI R&D,including pre-ignition and deto-knock,soot formation and PM emissions,injector deposits and gasoline compression ignition(GCI).First,an overview of recent developments in the field as driven by regulations is provided,following which progress in injection and combustion systems is examined.Third,the review addresses the occurrence and mechanism of deto-knock and considers means of suppressing this phenomenon.The fourth section discusses soot formation mechanisms and particulate matter emission characteristics of GDI engines and describes the application of gasoline particulate filter(GPF)after-treatment.The subsequent section summarizes studies regarding injector deposit formation,as well as pioneering research into GCI combustion modes.Finally,a summary and future prospects for GDI engine technologies are provided. | Shijin Shuai Xiao Ma Yanfei Li Yunliang Qi Hongming Xu | 2018 | Automotive Innovation2018，1，2： | 8 |
| 11 | An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens显示文摘Objective To provide a feasible and cost-effective next-generation sequencing(NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. Methods Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure: reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide(random hexamer or non-ribosomal hexanucleotide) primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline. Results NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did. Moreover, direct testing of clinical specimens using this improved hexanucleotide primer-based NGS approach provided further detailed genotypes of enteroviruses causing hand, foot, and mouth disease(HFMD) and identified other potential viruses or differentiated misdiagnosis events. Conclusion The improved barcoded oligonucleotide primer-based NGS approach is simplified, time saving, cost effective, and appropriate for direct identification of viral pathogens in clinical practice. | WANG Churl Hua NIE Kai ZHANG Yi WANG Ji ZHOU Shuai Feng LI Xin Na ZHOU Hang Yu QI Shun Xiang MA Xue Jun | 2017 | Biomedical and Environmental Sciences2017，30，1： | 7 |
| 12 | Nanoparticle monolayer-based flexible strain gauge with ultrafast dynamic response for acoustic vibration detection显示文摘The relatively poor dynamic response of current flexible strain gauges has prevented their wide adoption in portable electronics. In this work, we present a greatly improved flexible strain gauge, where one strip of Au nanoparticle (NP) monolayer assembled on a polyethylene terephthalate film is utilized as the active unit. The proposed flexible gauge is capable of responding to applied stimuli without detectable hysteresis via electron tunneling between adjacent nanoparticles within the Au NP monolayer. Based on experimental quantification of the time and frequency domain dependence of the electrical resistance of the proposed strain gauge, acoustic vibrations in the frequency range of 1 to 20,000 Hz could be reliably detected. In addition to being used to measure musical tone, audible speech, and creature vocalization, as demonstrated in this study, the ultrafast dynamic response of this flexible strain gauge can be used in a wide range of applications, including miniaturized vibratory sensors, safe entrance guard management systems, and ultrasensitive pressure sensors. | Lizhi Yi Weihong Jiao Ke Wu Lihua Qian Xunxing Yu Qi Xia Kuanmin Mao Songliu Yuan Shuai Wang Yingtao Jiang | 2015 | Nano Research2015，8，9： | 6 |
| 13 | Hydrothermal alteration of plagioclase microphenocrysts and glass in basalts from the East Pacific Rise near 13°N: An SEM-EDS study显示文摘The interactions of seafloor hydrothermal fluid with igneous rocks can result in leaching elements from the rocks,creating potential ore-forming fluids and influencing the chemical compositions of near-bottom seawater.The hydrothermal alteration of plagioclase microphenocrysts and basaltic glass in the pillow basalts from one dredge station(103°57.62′′W,12°50.55′N,water depth 2480 m)on the East Pacific Rise(EPR)near 13°N were analyzed using a scanning electron microscope(SEM)and energy dispersive X-ray spectrometry(EDS).The results show that the edges of the plagioclase microphenocrysts and the basaltic glass fragments are altered but the pyroxene and olivine microphenocrysts in the interior of the pillow basalts appear to be unaffected by the hydrothermal fluids.In addition,our results show that the chemical alteration at the rims of the plagioclase microphenocrysts and the edges of basaltic glass fragments can be divided into separate types of alteration.The chemical difference in hydrothermal alteration of the plagioclase microphenocrysts and the basaltic glass indicate that different degrees of hydrothermal fluid-solid phase interaction have taken place at the surface of the pillow basalts.If the degree of hydrothermal fluid-solid phase interaction is relatively minor,Si,Al,Ca and Na diffuse from the inside of the solid phase out and as a result these elements have a tendency to accumulate in the edge of the plagioclase microphenocrysts or basaltic glass.If the degree of hydrothermal fluid-solid phase interaction is relatively strong,Si,Al,Ca and Na also diffuse from the inside of solid phase out but these elements will have a relatively low concentration in the edge of the plagioclase microphenocrysts or basaltic glass.Based on the chemical variation observed in the edges of plagioclase microphenocrysts and basaltic glass,we estimate that the content of Si,Al and Fe in the edges of plagioclase microphenocrysts can have a variation of 10.69%,17.59%and 109%,respectively.Similarly,the Si,Al and Fe concentrations in the edges of basaltic glass can have a variation of 9.79%,16.30%and 37.83%,respectively,during the interaction of hydrothermal fluids and seafloor pillow basalt. | ZENG ZhiGang QI HaiYan CHEN Shuai YIN XueBo LI ZhaoXue | 2014 | Science China Earth Sciences2014，57，7： | 5 |
| 14 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 15 | RIP1-dependent linear and nonlinear recruitments of caspase-8 and RIP3 respectively to necrosome specify distinct cell death outcomes显示文摘There remains a significant gap in our quantitative understanding of crosstalk between apoptosis and necroptosis pathways.By employing the SWATH-MS technique,we quantified absolute amounts of up to thousands of proteins in dynamic assembling/de-assembling of TNF signaling complexes.Combining SWATH-MS-based network modeling and experimental validation,we found that when RIP1 level is below~1000 molecules/cell(mpc),the cell solely undergoes TRADD-dependent apoptosis.When RIP1 is above~1000 mpc,pro-caspase-8 and RIP3 are recruited to necrosome respectively with linear and nonlinear dependence on RIP1 amount,which well explains the co-occurrence of apoptosis and necroptosis and the paradoxical obser-vations that RIP1 is required for necroptosis but its increase down-regulates necroptosis.Higher amount of RIP1(>~46,000 mpc)suppresses apoptosis,leading to necroptosis alone.The relation between RIP1 level and occurrence of necroptosis or total cell death is biphasic.Our study provides a resource for encoding the com-plexity of TNF signaling and a quantitative picture how distinct dynamic interplay among proteins function as basis sets in signaling complexes,enabling RIP1 to play diverse roles in governing cell fate decisions. | Xiang Li Chuan-Qi Zhong Rui Wu Xiaozheng Xu Zhang-Hua Yang Shaowei Cai Xiurong Wu Xin Chen Zhiyong Yin Qingzu He Dianjie Li Fei Xu Yihua Yan Hong Qi Changchuan Xie Jianwei Shuai Jiahuai Han | 2021 | Protein & Cell2021，12，11： | 5 |
| 16 | MicroRNA and histopathological characterization of pure mucinous breast carcinoma显示文摘Objective: Pure mucinous breast carcinoma (PMBC) is an uncommon histological type of breast cancer characterized by a large amount of mucin production. MicroRNA (miRNA) is a large class of small noncoding RNA of about 22 nt involved in the regulation of various biological processes. This study aims to identify the miRNA expression profile in PMBC. Methods: MiRNA expression profiles in 11 PMBCs were analyzed by miRNA-microarray and real-time polymerase chain reaction (PCR). Thirty-one PMBCs and 27 invasive ductal carcinoma of no special types (IDC-NSTs) were assessed by immunohistochemistry using antibodies against ER, PR-progesterone receptor, HER2, Ki-67, Bcl-2, p53, PCNA, and CK5 and 6. Results: We analyzed the miRNA expression in 11 PMBCs and corresponding normal tissues using miRNA-microarray and real-time PCR, and found that miR-143 and miR-224-5p were significantly downregulated in mucinous carcinoma tissue. Compared with IDC-NSTs, PMBC showed a significantly higher ER positive rate, lower HER-2 positive rate, and lower cell proliferation rates. Conclusions: To our knowledge, this is the first study to demonstrate the miRNA expression profile of PMBC, and our findings may lead to further understanding of this type of breast cancer. | Feng Zhou Shuai Li Hui-Min Meng Li-Qiang Qi Lin Gu | 2013 | Cancer Biology & Medicine2013，10，1： | 5 |
| 17 | A Comparative Analysis of the Chloroplast Genomes of Four Salvia Medicinal Plants显示文摘Herbgenomics is an emerging field of traditional Chinese medicine(TCM)research and development.By combining TCM research with genomics,herbgenomics can help to establish the scientific validity of TCM and bring it into wider usage within the field of medicine.Salvia Linn.(S.Linn.)is a large genus of Labiatae that includes important medicinal plants.In this herbgenomics study,the complete chloroplast(cp)genomes of two Salvia spp.—namely,S.przewalskii and S.bulleyana,which are used as a surrogate for S.miltiorrhiza—were sequenced and compared with those of two other reported Salvia spp.—namely,S.miltiorrhiza and S.japonica.The genome organization,gene number,type,and repeat sequences were compared.The annotation results showed that both Salvia plants contain 114 unique genes,including 80 protein-coding,30 transfer RNA(tRNA),and four ribosomal RNA(rRNA)genes.Repeat sequence analysis revealed 21 forward and 22 palindromic sequences in both Salvia cp genomes,and 17 and 21 tandem repeats in S.przewalskii and S.bulleyana,respectively.A synteny comparison of the Salvia spp.cp genomes showed a high degree of sequence similarity in the coding regions and a relatively high divergence of the intergenic spacers.Pairwise alignment and single-nucleotide polymorphism(SNP)analyses found some candidate fragments to identify Salvia spp.,such as the intergenic region of the trnV–ndhC,trnQ–rps16,atpI–atpH,psbA–ycf3,ycf1,rpoC2,ndhF,matK,rpoB,rpoA,and accD genes.All of the results—including the repeat sequences and SNP sites,the inverted repeat(IR)region border,and the phylogenetic analysis—showed that S.przewalskii and S.bulleyana are extremely similar from a genetic standpoint.The cp genome sequences of the two Salvia spp.reported here will pave the way for breeding,species identification,phylogenetic evolution,and cp genetic engineering studies of Salvia medicinal plants. | Conglian Liang Lei Wang Juan Lei Baozhong Duan Weisi Ma Shuiming Xiao Haijun Qi Zhen Wang Yaoqi Liu Xiaofeng Shen Shuai Guo Haoyu Hu Jiang Xu Shilin Chen | 2019 | Engineering2019，5，5： | 5 |
| 18 | Fe-Si-Mn-Oxyhydroxide Encrustations on Basalts at East Pacific Rise near 13°N:An SEM-EDS Study显示文摘Fe-Si-Mn-oxyhydroxide encrustations at the East Pacific Rise(EPR) near 13°N were analyzed using the scanning electron microscope(SEM) with an energy dispersive spectrometer(EDS). These encrustations are mainly composed of amorphous FeSi-Mn-oxyhydroxides forming laminated, spherical, porous aggregates with some biodetritus, anhydrite, nontronite, and feldspar particles. Anhydrite particles and nontronite crystals in the Fe-Si-Mn-oxyhydroxide encrustations imply that the Fe-Si-Mn- oxyhydroxide may have formed under relatively low- to high-temperature hydrothermal conditions. The Fe-Si-Mn-oxyhydroxide encrustations on pillow basalts are 1–2 mm thick. The growth rate of ferromanganese crusts in the survey area suggests that these encrustations are an unlikely result of hydrogenic deposition alone having a hydrothermal and(Fe/Mn ratio up to 7.7 and Fe/(Fe+Mn+Al) ratio exceeding 0.78) hydrogenic origin(0.22 Fe/Mn ratio close to the mean value of 0.7 for open-ocean seamount crusts). The varying Fe/Mn ratios indicate that the Fe-Si-Mn-oxyhydroxide encrustations have formed through several stages of seafloor hydrothermalism. It is suggested that, at the initial formation stage, dense Fe-Si-oxyhydroxides with low Mn content deposit from a relatively reducing hydrothermal fluid, and then the loose Fe-Si-Mn-oxyhydroxides deposit on the Fe-Si-oxyhydroxides. As the oxidation degree of hydrothermal fluid increases and Si-oxide is inhibited, Mn-oxide will precipitate with Fe-oxyhydroxides. | WANG Xiaoyuan ZENG Zhigang QI Haiyan CHEN Shuai YIN Xuebo YANG Baoju | 2014 | Journal of Ocean University of China2014，13，6： | 5 |
| 19 | Single-nucleus transcriptomic landscape of primate hippocampal aging显示文摘The hippocampus plays a crucial role in learning and memory,and its progressive deterioration with age is functionally linked to a variety of human neurodegenerative diseases.Yet a systematic profiling of the aging effects on various hippocampal cell types in primates is still missing.Here,we reported a variety of new aging-associated phenotypic changes of the primate hippocampus.These include,in particular,increased DNA damage and heterochromatin erosion with time,alongside loss of proteostasis and elevated inflammation.To understand their cellular and molecular causes,we established the first single-nucleus transcriptomic atlas of primate hippocampal aging.Among the 12 identified cell types,neural transiently amplifying progenitor cell(TAPC)and microglia were most affected by aging.In-depth dissection of gene-expression dynamics revealed impaired TAPC division and compromised neuronal function along the neurogenesis trajectory;additionally elevated pro-inflammatory responses in the aged microglia and oligodendrocyte,as well as dysregulated coagulation pathways in the aged endothelial cells may contribute to a hostile microenvironment for neurogenesis.This rich resource for understanding primate hippocampal aging may provide potential diagnostic biomarkers and therapeutic interventions against age-related neurodegenerative diseases. | Hui Zhang Jiaming Li Jie Ren Shuhui Sun Shuai Ma Weiqi Zhang Yang Yu Yusheng Cai Kaowen Yan Wei Li Baoyang Hu Piu Chan Guo-Guang Zhao Juan Carlos Izpisua Belmonte Qi Zhou Jing Qu Si Wang Guang-Hui Liu | 2021 | Protein & Cell2021，12，9： | 5 |
| 20 | Research progress of nanoparticles as enzyme mimetics显示文摘Natural enzymes as biological catalysts possess remarkable advantages,especially their highly efficient and selective catalysis under mild conditions.However,most natural enzymes are proteins,thus exhibiting an inherent low durability to harsh reaction conditions.Artificial enzyme mimetics have been pursued extensively to avoid this drawback.Quite recently,some inorganic nanoparticles(NPs) have been found to exhibit unique enzyme mimetics.In addition,their much higher stability overcomes the inherent disadvantage of natural enzymes.Furthermore,easy mass-production and low cost endow them more benefits.As a new member of artificial enzyme mimetics,they have received intense attention.In this review article,major progress in this field is summarized and future perspectives are highlighted. | HU XiaoNa LIU JianBo HOU Shuai WEN Tao LIU WenQi ZHANG Ke HE WeiWei JI YingLu REN HongXuan WANG Qi WU XiaoChun | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，10： | 4 |
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