| 5 | Effect of extract from Yiyuan Yiliu Tang(益元抑瘤汤)on human lung adenocarcinoma and human hepatoma cell lines显示文摘OBJECTIVE:To investigate the efficacy of the extract from Yiyuan Yiliu Tang(益元抑瘤汤,YYYLT)on human lung adenocarcinoma cells A549 and human hepatoma cells Bel7402.METHODS:The cancer cell lines were treated with various concentrations(0,100,200,300,and400μg/m L)of the crude water extract of YYYLT and then cell viability,toxicity,cytokine secretion,and cell cycle/apoptosis were determined by MTT assay,LDH assay,and flow cytometry,respectively.RESULTS:The extract from YYYLT significantly suppressed the proliferation of the cancer cell lines and the release of interleukin-2 and tumor necrosis factor-αin a dose-dependent manner.The extract also promoted apoptosis,caused cell cycle arrest at G0/G1 phase,and increased the expression of caspase-3,B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X proteins.CONCLUSION:The extract from YYYLT might be a potential treatment for human lung and liver cancers. | Yl Qingqing CHANG Qing LIANG Dongyu CAI Weiwei QIU Liying YANG Rong JIANG Guowei | 2021 | Journal of Traditional Chinese Medicine2021，41，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员