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| 1 | A microRNA encoded by HSV-1 inhibits a cellular transcriptional repressor of viral immediate early and early genes显示文摘Viral microRNAs are one component of the RNA interference phenomenon generated during viral infection. They were first identified in the Herpesviridae family, where they were found to regulate viral mRNA translation. In addition, prior work has suggested that Kaposi's sarcoma-associated herpesvirus (KSHV) is capable of regulating cellular gene transcription by miRNA. We demonstrate that a miRNA, hsv1-mir-H27, encoded within the genome of herpes simplex virus 1 (HSV-1), targets the mRNA of the cellular transcriptional repressor Kelch-like 24 (KLHL24) that inhibits transcriptional efficiency of viral immediate-early and early genes. The viral miRNA is able to block the expression of KLHL24 in cells infected by HSV-1. Our discovery reveals an effective viral strategy for evading host cell defenses and supporting the efficient replication and proliferation of HSV-1. | WU WenJuan GUO ZhongPing ZHANG XueMei GUO Lei LIU LongDing LIAO Yun WANG JingJing WANG LiChun LI QiHan | 2013 | Science China(Life Sciences)2013，56，4： | 6 |
| 2 | Attenuated phenotypes and analysis of a herpes simplex virus 1strain with partial deletion of the UL7, UL41 and LAT genes显示文摘We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mutant, we further modified the UL41 gene, which encodes another tegument protein, and the latency-associated transcript(LAT) gene. Observations of the resulting mutants with modified UL7 and UL41(M2) or UL7, UL41 and LAT(M3) genes indicated attenuated phenotypes, with lower proliferative ratios in various cells, non-lethal infections in mice and lower viral loads in nervous tissues compared with the wild-type strain. Furthermore, no LAT stable intron could be detected in the trigeminal ganglion of M3-infected animals. The results obtained with the three HSV-1 mutants indicate that the M3 mutant is an attenuated strain with low pathogenicity during both acute and latent infections. Together, the results support the use of the M3 mutant as a candidate for the development of an HSV-1 vaccine. | Xingli Xu Yingqiu Guo Shengtao Fan Pingfang Cui Min Feng Lichun Wang Ying Zhang Yun Liao Xiaolong Zhang Qihan Li | 2017 | Virologica Sinica2017，32，5： | 6 |
| 3 | A comparative study of the characteristics of two Coxsackie A virus type 16 strains (genotype B)显示文摘Coxsackie A virus is one of the major pathogens associated with hand, foot and mouth disease (HFMD). The etiological characteristics of Coxsackie A virus type 16 (CA16) are thought to correlate with the pathological process of its infection. Two CA16 strains that were isolated from a severe HFMD patient presented with different plaque forms. This observation, along with biological analysis, indicated that the differences in the strains' biological characteristics, such as proliferation kinetics and immunogenicity, correlate with differences in their pathogenicity toward neonatal mice. Furthermore, these differences are thought to be associated with the sequence of the 5′ non-coding region of the viral genome and the VP1 structural region sequence. The results suggest that the biological and genetic characteristics of the CA16 viral strains are relevant to their pathogenicity. | YANG ErXia ZHAO Heng ZHANG Ying LIU JianSheng LIAO Yun WANG LiChun CUI PingFang YANG LiXian LIU LongDing DONG ChengHong DONG ShaoZhong SHAO CongWen JIANG Li SUN Le LI QiHan | 2012 | Science China(Life Sciences)2012，55，4： | 5 |
| 4 | Transcriptional regulation of the Herpes Simplex Virus 1α-gene by the viral immediate-early protein ICP22 in association with VP16显示文摘Herpes Simplex Virus 1 (HSV1) is capable of inducing two forms of infection in individuals, and the establishment of which type of infection occurs is linked to the transcriptional activation of viral α genes. One of the HSV1 α genes, ICP22, is known to have multiple functions during virus replication, but its distinct roles are still unclear. This study showed that ICP22 functions as a general repressor for certain viral and cellular promoters, and this transcriptional repression by ICP22 is independent of the specific upstream promoter element, as shown using the CAT enzyme assay system. Further work also found that VP16 interfered with ICP22 mediated transcriptional repression of the viral α4 gene, through interactions with specific elements upstream of the α4 gene promoter. These findings support the possibility that ICP22 and VP16 control transcription of HSV1α genes in a common pathway for the establishment of either viral lytic or latent infections. | CUN Wei, GUO Lei, ZHANG Ying, LIU LongDing, WANG LiChun, LI JianFeng, DONG ChengHong, WANG JinJin & LI QiHan Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | 2009 | Science China(Life Sciences)2009，52，4： | 4 |
| 5 | The miRNAs of Herpes Simplex Virus(HSV)显示文摘Herpes simplex virus (HSV) is a group of common human pathogens with two serotypes HSV-1 and HSV-2.The prevalence of HSV is worldwide.It primarily infects humans through epithelial cells,when it introduces a latent infection into the nervous system.During viral latency,only a region known as the latency-associated transcript (LAT) is expressed.The discovery of HSV miRNAs helps to draw a larger picture of the infection and pathogenesis of the virus.This review summarizes miRNAs found in HSV-1 and HSV-2 so far.The functional studies of miRNAs in HSV to date indicate that they play a stage-specific role coordinated with viral proteins to maintain the virus life cycle. | Le Sun Qihan Li | 2012 | Virologica Sinica2012，27，6： | 4 |
| 6 | HSV-1 stimulation-related protein HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2显示文摘The protein encoded by HSRG1(HSV-1 stimulation-related gene 1) is a virally induced protein expressed in HSV-1-infected cells.We have already reported that HSRG1 is capable of interacting with transcriptional regulator proteins.To further analyze the effects of HSRG1 on the regulation of viral gene transcription,we expressed the HSRG1 protein in transfected cells and found that it postpones the proliferation of HSV-1.CAT(chloramphenicol acetyltransferase) assays also revealed that HSRG1 reduces transcription from HSV-1 promoters.Yeast two-hybrid and immunoprecipitation assays indicated that HSRG1 interacts with Cyclin T2,the regulatory subunit of P-TEFb,which is required for transcription elongation by RNA Pol II(RNAP II) ,and that amino acid residues 1-420 in Cyclin T2 are important for binding with HSRG1.Fluorescence assays suggested that the cellular localizations of those two proteins are influenced by their interaction.Further analyses with CAT assays revealed that HSRG1 inhibits the transcriptional activation by Cyclin T2 of viral promoters.Our results suggested that the inhibitory effects of HSRG1 on viral replication and proliferation are probably induced by its binding to Cyclin T2.Therefore,it is likely that HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2. | WU WenJuan YU Xian LI WeiZhong GUO Lei LIU LongDing WANG LiChun LI QiHan | 2011 | Science China(Life Sciences)2011，54，4： | 3 |
| 7 | Analysis of HSV-I ICP22 effects on HCMV major immediate-early promoter structure显示文摘The human cytomegalovirus (HCMV) major immediate-early (MIE) promoter has strong transcriptional promoting capability. Its cis-acting regulatory elements form a special structure in this region that is repeated multiple times; the biological significance of these elements and their different compositions in the transcriptional promoting process remain unclear. Our results demonstrate that the HSV-I MIE protein ICP22 can generate strong repression of many viral and cellular promoters and enhancers. We further studied the transcriptional effects of ICP22 on structural elements and mutations in various HCMV MIE promoters by using a CAT assay. In spite of different transcriptional effects of all the ele- ments in the presence of ICP22, the transcriptional efficiencies exhibited by mutations generated by different compositions and an entire HCMV promoter, are not the simple sum of the functions of these elements. Furthermore, the transcriptional activities of specific sequences were not affected by the presence of ICP22. Therefore, it is assumed that the HCMV MIE promoter co-regulates expression of downstream genes by using viral and cellular specific factors via a specific pathway. | LUO Jie, CUN Wei, CHE YanChun, WANG LiChun, LI WeiZhong, LIU LongDing & LI QiHan Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China | 2007 | Science China(Life Sciences)2007，50，3： | 2 |
| 8 | Immediate-early gene product ICP22 inhibits the trans-transcription activating function of P53-mdm-2显示文摘As a product of HSVI immediate-early gene, ICP22 is capable of interacting with various cellular tran-scriptive and regulatory molecules during viral infection so as to impact the normal cellular molecular mechanism. ICP22 expressed in transfected cells can push the cells’ entering into S phase with binding to mdm-1 promoter region and impact its trans-transcription activating effect by P53. Consequently, the MDM-2 binds to P53, and the degradation effects by the ubiquitous pathway are decreased, improving indirectly the P53 levels in cells and making the cells progress into the S phase. | GUO HongXiong, CUN Wei, LIU LongDing, WANG LiChun, ZHAO HongLing, DONG ChengHong & LI QiHan Department of Viral Immunology, Institute of Medical Biology, CAMS and PUMC, Kunming 650118, China These authors contributed equally to this work | 2007 | Science China(Life Sciences)2007，50，4： | 2 |
| 9 | A cellular response protein induced during HSV-1 infection inhibits viral replication by interacting with ATF5显示文摘Studies of herpes simplex virus type 1(HSV-1)infection have shown that many known and unknown cellular molecules involved in viral proliferation are up-regulated following HSV-1 infection.In this study,using two-dimensional polyacrylamide gel electrophoresis,we found that the expression of the HSV-1 infection response repressive protein(HIRRP,GI 16552881)was up-regulated in human L02 cells infected with HSV-1.HIRRP,an unknown protein,was initially localized in the cytoplasm and then translocated into the nucleus of HSV-1-infected cells.Further analysis showed that HIRRP represses HSV-1proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor,ATF5,via its N-terminal domain.ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif.We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection;HIRRP then induces feedback repression of this transcription by interacting with ATF5. | WU LianQiu ZHANG XueMei CHE YanChun ZHANG Ying TANG SongQing LIAO Yun NA RuiXiong XIONG XiangLin LIU LongDing LI QiHan | 2013 | Science China(Life Sciences)2013，56，12： | 2 |
| 10 | A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein显示文摘Dear Editor,Since December 2019, the outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) induced human disease, the coronavirus disease 2019(COVID-19),has been reported in more than 200 countries and areas around the world(Wu et al. 2020). | Zhiqiang Chen Qihan Wu Jing Chen Xiaohua Ni Jianfeng Dai | 2020 | Virologica Sinica2020，35，3： | 2 |
| 11 | Robustness analysis of mistuned bladed disk using the upper bound of structured singular value显示文摘 | YAO Jianyao WANG Jianjun LI Qihan | 2009 | ASME Journal of Engineering for Gas Turbines and Power2009，131，03： | 1 |
| 12 | Discounting decisions in a suppler-buyer relationship with a linear buyer's demand 显示文摘 | Palar M Wang Qihan | 1994 | IIE Trans1994，26，2： | 1 |
| 13 | Significant elevation of B cells at the acute stage in enterovirus 71-infected children with central nervous system involvement显示文摘 | Jing Xie Yang Jiao Zhifeng Qiu Qihan Li Taisheng Li | 2010 | Scandinavian Journal of Infectious Diseases2010，，11： | 1 |
| 14 | Biomass Homogeneity Reinforced Carbon Aerogels Derived Functional Phase-Change Materials for Solar–Thermal Energy Conversion and Storage显示文摘We deviseda functional form stable compositephase-change materials(PCMs)toachieve a three-dimensional(3D)interconnectedporous carbon aerogel structure for encapsulating polyethyleneglycol(PEG).Anovelhomogeneity reinforced carbonaerogel witha well-interconnected porous structure was constructed bycombining a flexible carbonresource from biomass guar gum with hard-brittle carbonfrom polyimide,to overcome severeshrinkage andpoor mechanical performance of traditionalcarbon aerogel.Thesupportingcarbon aerogel-encapsulated PEG produced thenovel composite PCMswithgood structure stability andcomprehensive energy storage performance.Theresults showed thatthecomposite PCMsdisplayed awell-defined 3Dinterconnected structure,and theirenergy storage capacities were 171.5 and169.5 J/g,which changed onlyslightlyafter 100 thermalcycles,andthe compositescould maintainthe equilibrium temperature at50.0−58.1℃ for about 760.3 s.The thermal conductivityofthe compositescould reach0.62 W m^(−1) K^(−1),which effectively enhanced the thermalresponse rate.And thecomposite PCMs exhibited good leakage-proof performance andexcellent light–thermal conversion.The compressive strengthof thecomposite PCMscan improveupto 1.602 MPa.Results indicatethatthisstrategy canbe efficiently usedtodevelop novel composite PCMswithimproved comprehensive thermalperformance and high light–thermal conversion. | Qingfeng Zhang Tingfeng Xia Qihan Zhang Yucao Zhu Huanzhi Zhang Fen Xu Lixian Sun Xiaodong Wang Yongpeng Xia Xiangcheng Lin Hongliang Peng Pengru Huang Yongjin Zou Hailiang Chu Bin Li | 2023 | Energy & Environmental Materials2023，6，1： | 1 |
| 15 | Robustness analysis of mistuned bladed disk using the upper bound of structured singular value 显示文摘 | YAO Jianyao WANG Jianjun LI Qihan | 2009 | ASME Journal of Engineer ing for Gas Turbines and Power2009，131，03： | 1 |
| 16 | Waist - to - height ratio is an appropriate index for identifying cardiometabolic risk in Chinese individuals with normal body mass index and waist circumference显示文摘 | Qihan Zhu Feixia Shen | 2014 | Journal of Dia- betes2014，6，6： | 1 |
| 17 | Frequency Re- sponse Characteristics of Parametrically Excited System显示文摘 | Han Qinkai Wang Jianjun Li Qihan | 2010 | Journal of Vibration and Acoustics2010，132，： | 1 |
| 18 | Transmission of Point-line Meshing Gear显示文摘 | Luo Qihan Li Haixiang Wang Junrong | 2007 | The International Journal of Advanced Manufacturing Technology2007，33，10： | 1 |
| 19 | A positive solution of a nonlinear Schrdinger system with nonconstant potentials显示文摘In this paper, we study the existence of positive solutions to the following Schr¨odinger system:{-?u + V_1(x)u = μ_1(x)u^3+ β(x)v^2u, x ∈R^N,-?v + V_2(x)v = μ_2(x)v^3+ β(x)u^2v, x ∈R^N,u, v ∈H^1(R^N),where N = 1, 2, 3; V_1(x) and V_2(x) are positive and continuous, but may not be well-shaped; and μ_1(x), μ_2(x)and β(x) are continuous, but may not be positive or anti-well-shaped. We prove that the system has a positive solution when the coefficients Vi(x), μ_i(x)(i = 1, 2) and β(x) satisfy some additional conditions. | HE QiHan LUO Xiao | 2017 | Science China Mathematics2017，60，12： | 1 |
| 20 | Transmission of point - line meshing gear显示文摘 | Luo Qihan Li Haixiang Wang Junrong | 2007 | Aduanced Manufacturing Technology2007，33，910： | 1 |
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