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| 1 | DNA-based vaccination induces humoral and cellular immune responses against hepatitis B virus surface antigen in mice without activation of Cmyc显示文摘AIM To develop a safe and effective DNAvaccine for inducing humoral and cellularimmunological responses against hepatitis Bvirus surface antigen(HBsAg).METHODS BALB/c mice were inoculated withNV-HB/s,a recombinant plasmid that had beeninserted S gene of hepatitis B virus genome andcould express HBsAg in eukaryotes.HBsAgexpression was measured by ABC immunohis-tochemical assay,generation of anti-HBs byELISA and cytotoxic T lymphocyte(CTL),byMTT method,existence of vaccine DNA bySouthern blot hybridization and activation ofoncogene C-myc by in situ hybridization,RESULTS With NV-HB/s vaccination byintramuscular injection,anti-HBs was initiallypositive 2 weeks after inoculation while all micetested were HBsAg positive in the muscles.Thetiters and seroconversion rate of anti-HBs weresteadily increasing as time went on and weredose-dependent.All the mice inoculated with100 μg NV-HB/s were anti-HBs positive onemonth after inoculation,the titer was 1:1024 ormore.The humoral immune response was similarinduced by either intramuscular or intradermalinjection.CTL activities were much stronger(45.26%)in NV-HB/s DNA immunized mice as compared with those(only 6%)in plasma-derived HBsAg vaccine immunized mice.Twomonths after inoculation,all muscle sampleswere positive by Southern-blot hybridization forNV.HB/s DNA detection,but decreased to 25%and all were undetectable by in situhybridization after 6 months.No oncogene C-myc activation was found in the muscle ofinoculation site.CONCLUSION NV-HB/s could generatehumoral and cellular immunological responsesagainst HBsAg that had been safely expressed insitu by NV-HB/s vaccination. | Lian San Zhao Shan Qin Tao You Zhou Hong Tang Li Liu Bing Jun Lei | 2000 | World Journal of Gastroenterology2000，6，2： | 24 |
| 2 | Serum vascular endothelial growth factor is a potential biomarker of metastatic recurrence after curative resection of hepatocellular carcinoma显示文摘INTRODUCTIONHepatocellular carcinoma(HCC)is one of the mostcommon malignancies in China.To date,surgery is stillthe best solution to it.However,metastatic recurrencesafter curative hepatic resections are very common.Tang etal have reported that recurrence rate within 5 years | Qi Niu Zhao You Tang Zeng Chen Ma Lun Xiu Qin Lian Hai Zhang | 2000 | World Journal of Gastroenterology2000，6，4： | 20 |
| 3 | NGX6 inhibits cell invasion and adhesion through suppression of Wnt/β-catenin signal pathway in colon cancer显示文摘结肠癌是与增加的病态和死亡被联系的一个普通恶意的肿瘤。鼻咽的联系癌的基因(NGX6 ) 6 是肿瘤转移的新奇候选人 suppressor 基因,它在结肠癌是下面调整的。这研究被设计在人的结肠癌房间线的生长和侵略上调查 NGX6 的角色,并且阐明, HT-29 他们的行动的分子的机制。结果证明 NGX6 能禁止侵略和 HT-29 房间的细胞外的矩阵粘附并且恢复差距 junctional 房间的细胞间的通讯。而且, NGX6 能从原子核和细胞质压制 -catenin 的 translocation 到血浆膜,禁止 TCF4 抄本因素,和下面调整的活动 Wnt-direct-targeted 基因 c-myc, cyclin D1 和 COX-2 的表示。我们建议 NGX6 在结肠癌通过 Wnt 信号小径的抑制禁止房间侵略和粘附。 | Qin Guo Shourong Shen Mantian Liao Ping Lian Xiaoyan Wang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，7： | 13 |
| 4 | OsRFPH2-10, a RING-H2 Finger E3 Ubiquitin Ligase, Is Involved in Rice Antiviral Defense in the Early Stages of Rice dwarf virus Infection显示文摘 | Lifang Liu Lian Jin Xiahe Huang Yongtao Geng Feng Li Qingqing Qin Rui Wang Shaoyi Ji Shanshan Zhao Qi Xie Chunhong Wei Can Xie Biao Ding Yi Li | 2014 | Molecular Plant2014，7，6： | 11 |
| 5 | ANTI-HYPOXIA AND ANTI-OXIDATION EFFECTS OF AMINOPHYLLINE ON HUMAN WITH ACUTE HIGH-ALTITUDE EXPOSURE显示文摘Objective To investigate the anti-hypoxia and anti-oxidation effects of aminophylline on human with acute high- altitude exposure. Methods Totally 100 young male army members newly recruited from Sichuan province (400 meters above sea level) were enrolled. They were randomly divided into two groups: 50 in aminophylline group (A group) and 50 in control group (C group). A group and C group orally took aminophylline and placebo respectively for 10 days, 7 days before entering Lhasa (3 658 meters above sea level) by air and 3 days after it. Several parameters were measured at three time points: before drug taken, 7 days after drug taken, and 3 days after ascending high altitude. These parameters included serum levels of nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), hydrogen dioxide (H2O2), lactic acid (LA), as well as arterial oxygen saturation (SO2), arterial oxygen partial pressure (PaO2), and arterial carbon dioxide partial pressure (PaCO2). Statistical analysis was conducted to compare the difference between two groups with Stata 7.0 software system. Results There were no statistical differences between groups in hypoxia and oxidation indicators before and after drug taken in plain area. Three days after ascending high altitude, the serum levels of SOD, CAT, H2O2, LA, PaCO2 increased in both groups, yet to a much larger degree in C group than A group (P<0.01); and NO, SO2, PaO2 decreased more markedly in C group (P<0.05 for NO, P<0.0001 for SO2 and PaO2). Conclusion Aminophylline has significant anti-hypoxia and anti-oxidation effects at high altitude. | Bo Yang Guang-yi Wang Bin Chen Rong-bin Qin Si Lang Zha Xi Lian Chen | 2007 | Chinese Medical Sciences Journal2007，22，1： | 8 |
| 6 | Signaling pathways involved in ischemic stroke:molecular mechanisms and therapeutic interventions显示文摘Ischemic stroke is caused primarily by an interruption in cerebral blood flow,which induces severe neural injuries,and is one of the leading causes of death and disability worldwide.Thus,it is of great necessity to further detailly elucidate the mechanisms of ischemic stroke and find out new therapies against the disease.In recent years,efforts have been made to understand the pathophysiology of ischemic stroke,including cellular excitotoxicity,oxidative stress,cell death processes,and neuroinflammation.In the meantime,a plethora of signaling pathways,either detrimental or neuroprotective,are also highly involved in the forementioned pathophysiology.These pathways are closely intertwined and form a complex signaling network.Also,these signaling pathways reveal therapeutic potential,as targeting these signaling pathways could possibly serve as therapeutic approaches against ischemic stroke.In this review,we describe the signaling pathways involved in ischemic stroke and categorize them based on the pathophysiological processes they participate in.Therapeutic approaches targeting these signaling pathways,which are associated with the pathophysiology mentioned above,are also discussed.Meanwhile,clinical trials regarding ischemic stroke,which potentially target the pathophysiology and the signaling pathways involved,are summarized in details.Conclusively,this review elucidated potential molecular mechanisms and related signaling pathways underlying ischemic stroke,and summarize the therapeutic approaches targeted various pathophysiology,with particular reference to clinical trials and future prospects for treating ischemic stroke. | Chuan Qin Sheng Yang Yun-Hui Chu Hang Zhang Xiao-Wei Pang Lian Chen Luo-Qi Zhou Man Chen Dai-Shi Tian Wei Wang | 2022 | Signal Transduction and Targeted Therapy2022，7，8： | 7 |
| 7 | Expression of proliferating cell nuclear antigen in polyps from large intestine显示文摘Coloncancerhasahighincidenceintheworld,especialyinWesterncountries,andtheincidenceisalsoincreasinginChinainrecentyears.Toredu... | LUO Yu Qin 1, MA Lian Sheng 2, ZHAO Yi Ling 3, WU Kai Chun 4, PAN Bo Rong 5 and ZHANG Xue Yong 4 | 1999 | World Journal of Gastroenterology1999，5，2： | 6 |
| 8 | Protective effect of YHI and HHI-I against experimental acute pancreatitis in rabbits显示文摘ProtectiveefectofYHIandHHIIagainstexperimentalacutepancreatitisinrabbitsZHAOLianGen,WUXiaoXian,HANEnKun,CHENYuLing,CHENC... | ZHAO Lian Gen, WU Xiao Xian, HAN En Kun, CHEN Yu Ling, CHEN Chi and XU Dong Qin | 1998 | World Journal of Gastroenterology1998，4，3： | 6 |
| 9 | An interface shear damage model of chromium coating/steel substrate under thermal erosion load显示文摘The Cr-plated coating inside a gun barrel can effectively improve the barrel’s erosion resistance and thus increase the service life.However,due to the cyclic thermal load caused by high-temperature gunpowder,micro-element damage tends to occur within the Cr coating/steel substrate interface,leading to a gradual deterioration in macro-mechanical properties for the material in the related region.In order to mimic this cyclic thermal load and,thereby,study the thermal erosion behavior of the Cr coating on the barrel’s inner wall,a laser emitter is utilized in the current study.With the help of in-situ tensile test and finite element simulation results,a shear stress distribution law of the Cr coating/steel substrate and a change law of the interface ultimate shear strength are identified.Studies have shown that the Cr coating/steel substrate interface’s ultimate shear strength has a significant weakening effect due to increasing temperature.In this study,the interfacial ultimate shear strength decreases from 2.57 GPa(no erosion)to 1.02 GPa(laser power is 160 W).The data from this experiment is employed to establish a Cr coating/steel substrate interface shear damage model.And this model is used to predict the flaking process of Cr coating by finite element method.The simulation results show that the increase of coating crack spacing and coating thickness will increase the service life of gun barrel. | Xiao-long Li Yong Zang Yong Lian Min-yu Ma Lei Mu Qin Qin | 2021 | Defence Technology(防务技术)2021，17，2： | 6 |
| 10 | Fiber Traction Printing:A 3D Printing Method of Continuous Fiber Reinforced Metal Matrix Composite显示文摘A novel metal matrix composite freeform fabrication approach,fiber traction printing(FTP),is demonstrated through controlling the wetting behavior between fibers and the matrix.This process utilizes the fiber bundle to control the cross-sectional shape of the liquid metal,shaping it from circular to rectangular which is more precise.The FTP process could resolve manufacturing difficulties in the complex structure of continuous fiber reinforced metal matrix composites.The printing of the first layer monofilament is discussed in detail,and the effects of the fibrous coating thickness on the mechanical properties and microstructures of the composite are also investigated in this paper.The composite material prepared by the FTP process has a tensile strength of 235.2 MPa,which is close to that of composites fabricated by conventional processes.The complex structures are printed to demonstrate the advantages and innovations of this approach.Moreover,the FTP method is suited to other material systems with good wettability,such as modified carbon fiber,surfactants,and aluminum alloys. | Xin Wang Xiaoyong Tian Qin Lian Dichen Li | 2020 | Chinese Journal of Mechanical Engineering2020，33，2： | 5 |
| 11 | Clinical and experimental study of therapeutic effect of Weixibaonizhuan pills on gastric precancerous lesions显示文摘ClinicalandexperimentalstudyoftherapeuticefectofWeixibaonizhuanpilsongastricprecancerouslesionsZHANGXuChen1,GAORuiFeng2,LI... | ZHANG XuChen1, GAO RuiFeng2, LI BingQing2, MA LianSheng3, MEI LiXin1, WU YuZhen2, LIU FengQin2 and Liao ZhengLin2 | 1998 | World Journal of Gastroenterology1998，4，1： | 5 |
| 12 | A pure blue light emitting binaphthyl derivative:Synthesis and properties显示文摘有 3 和 3 个′位置上的分核的联萘衍生物被综合并且经由铃木描绘了偶合反应。在答案最大的排放被作为参考拿 9,10-diphenyl 蒽与 68% 的量子效率在 390 nm 定位,当它与从聚集状态导致固态膜的 104 nm 的 FWHM 被转移到 450 nm 时。玻璃化转变温度(Tg ) 和分解温度被测量分别地,由 DSC 和 TGA 是 184 和 447 ° C。不同于它的光致发光光谱,电镀物品发光光谱在大约 460 nm 和表演达到顶点相应于纯蓝排出物的 69 nm 的 FWHM。刺激电压,发光性和效率最大值是有并列的 CIE 颜色的 5 V, 2953 cd/m2 和 1.37 cd/A (0.16, 0.15 ) ,在 ITO/NPB (40 nm ) 的设备结构 /PY-BN-PY (15 nm )/BPhen (40 nm )/Mg :47 号元素银的化学符号。 | Qing Guo He Zeng Ze Chu Gang Tie Lei An Jun Qin Hong Zhen Lin Feng Lian Bai Jian Gong Cheng Yong Qiu | 2008 | Chinese Chemical Letters2008，19，4： | 4 |
| 13 | Alteration of tumor suppressor gene p16 and Rb in gastric cancinogesis显示文摘Alterationoftumorsuppressorgenep16andRbingastriccancinogesisZHOUQi1,ZOUJianXiang2,CHENYuLong2,YUHuiZhen3,WANGLiDong1,LIYo... | ZHOU Qi1, ZOU JianXiang2, CHEN YuLong2, YU HuiZhen3,WANG LiDong1, LI YongXin1, GUO HuaQin1, GAO ShanShan1, and QIU SongLian11Laboratory for Cancer Research, Medical Experimental Center, 2Department of Gas | 1997 | World Journal of Gastroenterology1997，3，4： | 4 |
| 14 | A new technology for hard-rock tunneling based on drilling and hydraulic impact breaking显示文摘In order to realize the safety, high efficiency and rapidity of hard rock tunneling, we propose the drilling &hydraulic impact hard-rock tunneling(DHIHT) technology and method. Based on the LS-DYNA explicit module and APDL programming, the key parameters of DHIHT, including drilling spacing and drilling radius, were investigated. The simulation results show that: the drilling spacing should not exceed0.20 m-larger distances weaken the actual breaking effect; the best drilling radius is about 0.035 m, larger or smaller distances would both decrease the effect of drilling-hole free surfaces. The field impact breaking experiments were conducted in Baitaizi township granite quarry, Jinzhou, Liaoning province,China. The experiment results indicate that DHIHT is a feasible method for hard-rock tunneling, but its efficiency still needs to be further optimized and improved. | Long Risheng Sun Shaoni Lian Zisheng Liao Yaoyao Qin Xiaofeng | 2015 | International Journal of Mining Science and Technology2015，25，6： | 4 |
| 15 | Combined treatment of oxaliplatin and capecitabine in patients with metastatic esophageal squamous cell cancer显示文摘AIM:To investigate the efficacy and side effects of the combined therapy of oxaliplatin and capecitabine in patients with metastatic esophageal squamous cell cancer(ESCC) and the survival of the patients.METHODS:Sixty-four patients(median age of 63 years) with histological or cytological confirmation of ESCC received oxaliplatin 120 mg/m2 intravenously on day 1 and capecitabine 1000 mg/m2 orally twice daily on days 1 to 14 in a 21-d treatment cycle as palliative chemotherapy.Each patient received at least two cycles of treatment.The efficacy,side effects and patient survival were evaluated.RESULTS:The partial response(PR) rate was 43.8%(28/64).Stable disease(SD) rate was 47.9%(26/64),and disease progression rate was 15.6%(10/64).The clinical benefit rate(PR + SD) was 84.4%.The main toxicities were leukopenia(50.0%),nausea and vomiting(51.6%),diarrhea(50.0%),stomatitis(39.1%),polyneuropathy(37.5%) and hand-foot syndrome(37.5%).No grade 4 event in the entire cohort was found.The median progression-free survival was 4 mo,median overall survival was 10 mo(95% CI:8.3-11.7 mo),and the 1-and 2-year survival rates were 38.1% and 8.2%,respectively.High Karnofsky index,single metastatic lesion and response to the regimen indicated respectively good prognosis.CONCLUSION:Oxaliplatin plus capecitabine regimen is effective and tolerable in metastatic ESCC patients.The regimen has improved the survival moderately and merits further studies. | Tian-Jie Qin Gai-Li An Xin-Han Zhao Fang Tian Xiao-Hua Li Juan-Wen Lian Bo-Rong Pan Shan-Zhi Gu | 2009 | World Journal of Gastroenterology2009，15，7： | 4 |
| 16 | Immunological Evaluation of a Novel Mycobacterium tuberculosis Antigen Rv0674显示文摘Objective This study aimed to characterize the diagnostic and vaccine potential of a novel Mycobacterium tuberculosisantigen Rv0674. Methods To evaluate thediagnostic potential and antigenicity of Rv0674, IgG was evaluated using ELISA and interferon (IFN)-γ was done by using ELISpot assay among TB patients and healthy donors. For immunogenicity evaluation, BALB/c mice were immunized with Rv0674. Cytokine production was determined by cytokine release assay using an ELISA kit, and the antibodies were tested using ELISA. Results The results of serum Elisa tests showed that Rv0674 specific immunoglobulin G (IgG) response was higher in TB patients than negative controls. And Rv0674 had good performance in serological test with sensitivity and specificity of 77.1% and 81.1%, respectively. While it shows poor sensitivity and specificity of 26.23% and 79.69% for IFN-γ tests. In BALB/c mice, Rv0674 adjuvant by DDA/PolyI:C could also induce a high level of IFN-γ, interleukin-2 and interleukin-6 as well as a high IgG titer in both high-and low-dose groups indicating that Rv0674 is essential in humoral and cellular immunity. Moreover, the cytokine profile and IgG isotypecharacterized Rv0674 as a Th1/Th2-mixed-type protective immunity with the predominance of Th1 cytokines. Conclusion Rv0674 may be a good potential candidate for the development of TB serological diagnosis and a new TB vaccine. | XIAO Tong Yang LIU Hai Can LI Xiao Qin HUANG Ming Xiang LI Gui Lian LI Na YAN Yu Han LUO Qiao WANG Xue Zhi LI Ma Chao WAN Kang Lin | 2019 | Biomedical and Environmental Sciences2019，32，6： | 3 |
| 17 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 18 | Study on the Microstructure of Human Articular Cartilage/Bone Interface显示文摘 | Yaxiong Liu Qin Lian Jiankang He Jinna Zhao Zhongmin Jin Dichen Li | 2011 | Journal of Bionic Engineering2011，8，3： | 3 |
| 19 | An Ant Colony Optimization Based Dimension Reduction Method for High-Dimensional Datasets显示文摘 | Ying Li Gang Wang Huiling Chen Lian Shi Lei Qin | 2013 | Journal of Bionic Engineering2013，10，2： | 3 |
| 20 | Patient-Specific Design and Biomechanical Evaluation of a Novel Bipolar Femoral Hemi-Knee Prosthesis显示文摘 | Qin Lian Dichen Li Zhongmin Jin ZhenWang Yuhan Sun | 2014 | Journal of Bionic Engineering2014，11，2： | 3 |
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