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| 1 | Adenovirus-mediated CTLA4Ig gene inhibits infiltration of immune cells and cell apoptosis in rats after liver transplantation显示文摘AIM: To investigate the role of adenovirus-mediated CTLA4Ig gene therapy in inhibiting the infiltration of macrophages and CD8+T cells and cell apoptosis after liver transplantation. METHODS: The rat orthotopic liver transplantation model was applied. The rats were divided into three groups: group I: rejection control (SD-to-Wistar); group Ⅱ: acute rejection treated with intramuscular injection of CsA 3.0 mg/(kg·d) for 12 d (SD-to-Wistar+CsA); groupⅢ: injection of 1×10^9 PFU adenovirus-mediated CTLA41g gene liquor in dorsal vein of penis 7 d before liver transplantation (SD-to-Wistar+CTLA4Ig). Immunohistochemistry and transferase-mediated dUTP nick-end labeling (TUNEL )were used to analyze the expression of CTLA4Ig gene in liver, infiltration of macrophages and CD8+T cells, cell apoptosis in grafts at different time-points after liver transplantation. Histopathological examination was done. RESULTS: CTLA4Ig gene expression was positive in liver on d 7 after administering adenovirus-mediated CTLA4Ig gene via vein, and remained positive until day 60 after liver transplantation. Infiltration of macrophages and CD8+T cells in CTLA4Ig-treated group was less than in rejection control group and CsA-treated group. The apoptotic index of rejection group on d 3, 5, and 7 were significantly higher than that of CTLA4Ig-treated group. A good correlation was found between severity of rejection reaction and infiltration of immune activator cells or cell apoptotic index in grafts. CONCLUSION: CTEA4Ig gene is constantly expressed in liver and plays an important role in inducing immune tolerance, | Guo-Ping Jiang, Zhen-Hua Hu, Shu-Sen Zheng, Chang-Ku Jia, Ai-Bin Zhang, Wei-Lin Wang, Department of Hepatobiliary Pancreatic Surgery, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China | 2005 | World Journal of Gastroenterology2005，11，7： | 14 |
| 2 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 3 | Chain-type wireless sensor network node scheduling strategy显示文摘In order to reduce power consumption of sensor nodes and extend network survival time in the wireless sensor network(WSN), sensor nodes are scheduled in an active or dormant mode. A chain-type WSN is fundamentally different from other types of WSNs, in which the sensor nodes are deployed along elongated geographic areas and form a chain-type network topology structure. This paper investigates the node scheduling problem in the chain-type WSN. Firstly, a node dormant scheduling mode is analyzed theoretically from geographic coverage, and then three neighboring nodes scheduling criteria are proposed. Secondly, a hybrid coverage scheduling algorithm and dead areas are presented. Finally, node scheduling in mine tunnel WSN with uniform deployment(UD), non-uniform deployment(NUD) and optimal distribution point spacing(ODS) is simulated. The results show that the node scheduling with UD and NUD, especially NUD,can effectively extend the network survival time. Therefore, a strategy of adding a few mobile nodes which activate the network in dead areas is proposed, which can further extend the network survival time by balancing the energy consumption of nodes. | Guangzhu Chen Qingchun Meng Lei Zhang | 2014 | Journal of Systems Engineering and Electronics2014，25，2： | 9 |
| 4 | Design and test of robotic harvesting system for cherry tomato显示文摘Harvesting of fresh-eating cherry tomato was highly costly on labor and time.In order to achieve mechanical harvesting for the fresh-eating tomato,a new harvesting robot was designed,which consisted of a stereo visual unit,an end-effector,manipulator,a fruit collector,and a railed vehicle.The robot configuration and workflow design focused on the special cultivating condition.Three key parts were introduced in detail:a railroad vehicle capably moving on both ground and rail was adopted as the robot’s carrier,a visual servo unit was used to identify and locate the mature fruits bunch,and the end-effector to hold and separate the fruit bunch was designed based on the stalk’s mechanical features.The field test of the new developed robot was conducted and the results were analyzed.The successful harvest rate of the robot was 83%,however,each successful harvest averagely needed 1.4 times attempt,and a single successful harvesting cycle cost 8 s excluding the time cost on moving. | Qingchun Feng Wei Zou Pengfei Fan Chunfeng Zhang Xiu Wang | 2018 | International Journal of Agricultural and Biological Engineering2018，11，1： | 8 |
| 5 | Tracing the settlement region of massive floating green algae in the Yellow Sea显示文摘Outbreaks of large-scale green tides formed by Ulva prolifera in the Yellow Sea (YS) cause heavy ecological damages and huge economic losses. However, the ecological consequences of green tides remain poorly understood due to the lack of knowledge on the settlement region of massive green algae floating in the YS. In this study, we established a method to trace the settlement region of floating green algae, using 28-isofucosterol preserved in sediment as the specific biomarker for green algae. Sterols including 28-isofucosterol in surface sediment samples collected during an investigation in the YS and the Bohai Sea (BS) in August 2015 were analyzed using gas chromatography coupled with a mass spectrometer (GC-MS). Based on the content of 28-isofucosterol in sediment samples, the potential settlement region of fl oating green algae was identifi ed in the sea area southeast to the Shandong Peninsula around the sampling site H06 (122.66°E, 36.00°N). This paper presents a first result on the settlement region of floating green algae in the YS for providing a solid basis to elucidate the ecological consequences of green tides in the area. | GENG Huixia YU Rencheng ZHANG Qingchun YAN Tian KONG Fanzhou ZHOU Mingjiang | 2019 | Journal of Oceanology and Limnology2019，37，5： | 7 |
| 6 | Fuzzy sliding mode control guidance law with terminal impact angle and acceleration constraints显示文摘In this paper, a novel fuzzy sliding mode control(FSMC) guidance law with terminal constraints of miss distance, impact angle and acceleration is presented for a constant speed missile against the stationary or slowly moving target. The proposed guidance law combines the sliding mode control algorithm with a fuzzy logic control scheme for the lag-free system and the first-order lag system. Through using Lyapunov stability theory, we prove the sliding surface converges to zero in finite time. Furthermore, considering the uncertain information and system disturbances, the guidance gains are on-line optimized by fuzzy logic technique. Numerical simulations are performed to demonstrate the performance of the FSMC guidance law and the results illustrate the validity and effectiveness of the proposed guidance law. | Qingchun Li Wensheng Zhang Gang Han Yuan Xie | 2016 | Journal of Systems Engineering and Electronics2016，27，3： | 6 |
| 7 | A Principle of Astronomical Three-dimensional Position Fixing Based on Single Baseline Radio Interferometry显示文摘 | WANG Anguo LI Hui WANG Yan ZHANG Lei CAO Qingchun | 2011 | Chinese Journal of Electronics2011，20，1： | 5 |
| 8 | Application of digital pattern-less molding technology to produce art casting显示文摘Compared with the conventional casting process, digital pattern-less casting technology has many advantages such as good machining accuracy, a short processing cycle, and low production cost. It is a new rapid manufacturing technology for castings, integrated with CAD/CAM, casting, CNC machining and many other advanced technologies. With this digital casting technology, no pattern is needed for making molds; it is precise, f lexible, and green. Usually, art castings have complex structures and are made in small batches or even made in a single-piece, especially for large-sized art castings. So it has the shortcomings of high cost, low eff iciency and long time for making a pattern to produce art castings with the conventional casting processes. However, the digital pattern-less casting technology can be applied to fabricate art castings, since it can greatly shorten the manufacturing cycle and lower the production cost, thus having a very good prospect. In this study, based on the digital pattern-less casting technology, a plaque casting with artistic Chinese characters(a Chinese poem) was designed and manufactured, and the production process was demonstrated in detail. | Chen Li Zhang Wei Wu Chenhong Jia Baochang Xiang Qingchun Qiu Keqiang Li Rongde | 2014 | China Foundry2014，11，6： | 5 |
| 9 | Design and Experiment of ф-type-knots Knotters on Chinese Small Square Balers显示文摘Since the knotters on the Chinese rectangular balers are imported from outside of the country,Chinese knotters with independent intellectual property rights is far away from being closed.In order to harvest a large quantity of straw in a short period on the small-scale lands of China,basic requirements on the knotters are summarized.Mathematical model of the knotter is also determined uniquely.Furthermore,theф-type-knots knotter equipped on the Chinese square baler to form theфtype knots is designed.Knotting rate experiments of theф-type-knots knotter on the test bench and in the wheat/maize straws covered fields are carried out to check the knotting performances of the knotter.The parameters of the formed knots are also tested.The experiments results show that the knotting rate of theф-type-knots knotter reaches 100.0%on the test bench without straws,while reaches 99.6%in the wheat straws covered field and 100.0%in the maize straws covered field.The average maximum force in the knotting process is 194.7 N in the lab experiment.The length out of the knots formed in lab is 15.9%–20.6%lower than the knots formed in the field experiment.The breaking force of the knots formed in the field is 115.9%–167.2%higher than the knots formed in lab due to the higher preload and interactions with the compacted bales.Highly relevant relationships exist between the breaking force of the formed knots and the maximum force in the forming process of the knots in the lab experiment.The designed knotter breaks out the embarrassing situation of the domestic knotters which don’t have independent intellectual property rights,and promotes the development of Chinese knotter technology,and the mathematical model is helpful for designing new type of knotters. | LI Hui HE Jin WANG Qingjie LI Hongwen RASAILY Rabi Gautam CAO Qingchun ZHANG Xiangcai | 2014 | Chinese Journal of Mechanical Engineering2014，27，1： | 5 |
| 10 | A NEW DAMAGE MECHANICS BASED APPROACH TO FATIGUE LIFE PREDICTION AND ITS ENGINEERING APPLICATION显示文摘An approach based on continuum damage mechanics to fatigue life prediction for structures is proposed. A new fatigue damage evolution equation is developed, in which the parameters are obtained in a simple way with reference to the experimental results of fatigue tests on standard specimens. With the utilization of APDL language on the ANSYS platform, a finite element implementation is presented to perform coupling operation on damage evolution of material and stress redistribution. The fatigue lives of some notched specimens and a Pitch-change-link are predicted by using the above approach. The calculated results are validated with experimental data. | Fei Shen Weiping Hu Qingchun Meng Miao Zhang | 2015 | Acta Mechanica Solida Sinica2015，28，5： | 4 |
| 11 | β-catenin activation in hair follicle dermal stem cells in duces ectopic hair outgrowth and skin fibrosis显示文摘Hair follicle dermal sheath(DS)harbors hair follicle dermal stem cells(hfDSCs),which can be recruited to replenish DS and dermal papilla(DP).Cultured DS cells can differentiate into various cell lineages in vitro.However,it is unclear how its plasticity is modulated in vivo.Wnt/β-catenin signaling plays an important role in maintaining stem cells of various lineages and is required for HF development and regeneration.Here we report that activation ofβ-catenin in DS generates ectopic HF outgrowth(EF)by reprogramming HF epidermal cells and DS cells themselves,and endows DS cells with hair inducing ability.Epidermal homeostasis of pre-existing HFs is disrupted.Additionally,cell-autonomous progressive skin fibrosis is prominent in dermis,where the excessive fibroblasts largely originate from DS.Gene expression analysis of purified DS cells with activatedβ-catenin revealed significantly increased expression of Bmp,Fgf,and Notch ligands and administration of Bmp,Fgf,or Notch signaling inhibitor attenuates EF formation.In summary,our findings advance the current knowledge of high plasticity of DS cells and provide an insight into understanding how Wnt/β-catenin signaling controls DS cell behaviors. | Yixin Tao Qingchun Yang Lei Wang Jie Zhang Xuming Zhu Qianqian Sun Yunbin Han Qian Luo Yushu Wang Xizhi Guo Ji Wu Baojie Li Xiao Yang Lin He Gang Ma | 2019 | Journal of Molecular Cell Biology2019，11，1： | 4 |
| 12 | A DAMAGE MECHANICS MODEL FOR FATIGUE LIFE PREDICTION OF FIBER REINFORCED POLYMER COMPOSITE LAMINA显示文摘A damage mechanics fatigue life prediction model for the fiber reinforced polymer lamina is established. The stiffness matrix of the lamina is derived by elastic constants of fiber and matrix. Two independent damage degrees of fiber and matrix are introduced to establish constitutive relations with damage. The damage driving forces and damage evolution equations for fiber and matrix are derived respectively. Fatigue tests on 0°and 90°unidirectional laminates are conducted respectively to identify parameters in damage evolution equations of fiber and matrix. The failure criterion of the lamina is presented. Finally, the life prediction model for lamina is proposed. | Wenjing Shi Weiping Hu Miao Zhang Qingchun Meng | 2011 | Acta Mechanica Solida Sinica2011，24，5： | 4 |
| 13 | Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa显示文摘Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world.The morphological,physiological,and biochemical characteristics are different among geographic strains,which can not be distinguished with nuclear ribosomal DNA markers at present.Therefore,the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1–D2 and ITS regions,and three chloroplast intergenic spacers(petN-trnS1,trnM1-psbA,and rbcS-rpl27)were analyzed and compared among 13 strains of P.globosa isolated from the Pacific Ocean and Atlantic Ocean in this study.In addition,the nucleotide polymorphisms of 28S rDNA D1–D2,ITS,and rbcS-rpl27 regions were evaluated in two P.globosa strains.The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1–D2 region and ITS region,but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer.A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer,which had the strongest distinction to geographic strains compared to those of 28S rDNA D1–D2 and ITS regions.In the phylogenetic tree of rbcS-rpl27 intergenic spacer,the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P.globosa,and then formed an independent clade,while the other Atlantic strains and all of Pacific strains joined up to build the other clade.It was implied that at least two genetically distant populations of P.globosa existed in the Atlantic coastal regions.This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P.globosa,and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P.globosa. | Qingchun ZHANG Zhuang NIU Jinxiu WANG Chao LIU Fanzhou KONG Xiaokun HU Jiayu ZHAO Rencheng YU | 2021 | Journal of Oceanology and Limnology2021，39，2： | 3 |
| 14 | MICROSTRUCTURE AND PROPERTY OF Al/TiC COMPOSITES PREPARED BY REACTION SYNTHESIS显示文摘A novel in-situ process-Reaction Synthesis has been used to fabricate Al/TiC compos-ites,the phase constitute,microstructure and mechanical property of the Al/TiC composite have been investigated by the use of XRD and SEM.It has been shown that the reaction synthesized TiC particu-late is submicron in size(about 0.1~0.8μpm),spherical in shape and smooth in surface.In macrostruc-ture the distribution of the TiC particulate in matrix is homogeneous,but in micostructure there is micro-segregation of the TiC particulate at the intergrannular.The cast structure become fine because of the existence of the TiC particulate.In addition«with the increasing of weight percentage of TiC partic-ulate in alloy,the yield strength and ultimate tensile strength of the composite increase significantly«e.g.at 15%TiC,117% and 103% increase respectively,in the same time the elongation of the composite is still more than 4.5%.In the end,the analysis has been done by SEM on the fracture morphology of the composite.The results show that the fracture is ductility,and mainly because of the cluster of TiC particulate to intergrannular and the synthesis of bar morphology Al_(3)Ti. | Zhang Erlin Zeng Xiaochun Zeng Songyan Li Qingchun | 1996 | Transactions of Nonferrous Metals Society of China1996，6，1： | 3 |
| 15 | Volumetric modulation arc radiotherapy with flattening filter-free beams compared with conventional beams for nasopharyngeal carcinoma:a feasibility study显示文摘There is increasing interest in the clinical use of flattening filter-free(FFF) beams.In this study,we aimed to investigate the dosimetric characteristics of volumetric modulated arc radiotherapy(VMAT) with FFF beams for nasopharyngeal carcinoma(NPC).Ten NPC patients were randomly selected to undergo a RapidArc plan with either FFF beams(RA-FFF) or conventional beams(RA-C).The doses to the planning target volumes(PTVs),organs at risk(OARs),and normal tissues were compared.The technical delivery parameters for RapidArc plans were also assessed to compare the characteristics of FFF and conventional beams.Both techniques delivered adequate doses to PTVs.For PTVs,RA-C delivered lower maximum and mean doses and improved conformity and homogeneity compared with RA-FFF.Both techniques provided similar maximum doses to the optic nerves and lenses.For the brain stem,spinal cord,larynx,parotid glands,oral cavity,and skin,RA-FFF showed significant dose increases compared to RA-C.The dose to normal tissue was lower in RA-FFF.The monitor units(MUs) were(536 ± 46) MU for RA-FFF and(501± 25) MU for RA-C.The treatment duration did not significantly differbetween plans.Although both treatment plans could meet clinical needs,RA-C is dosimetrically superior to RA-FFF for NPC radiotherapy. | Mingzan Zhuang Tuodan Zhang Zhijian Chen Zhixiong Lin Derui Li Xun Peng Qingchun Qiu Renhua Wu | 2013 | Chinese Journal of Cancer2013，32，7： | 3 |
| 16 | FORMATION MECHANISM OF TiC IN Al/TiC COMPOSITES PREPARED BY DIRECT REACTION SYNTHESIS显示文摘FORMATIONMECHANISMOFTiCINAl/TiCCOMPOSITESPREPAREDBYDIRECTREACTIONSYNTHESIS①ZhangErlin,YangBo,ZengSongyanandLiQingchunNational... | Zhang, Erlin Bo, Yang Zeng, Songyan Li, Qingchun Ma, Mingzhen | 1998 | 中国有色金属学会会刊:英文版1998，8，1： | 3 |
| 17 | The potent different risk factors for cerebral infarction in young patients with and without type 2 diabetes: Subanalysis of the Young Cerebral Infarction Study (YCIS)显示文摘 | Bin Zhang Cong Gao Qinghua Hou Jianrui Yin Longchang Xie Shuxiang Pu Yonghong Yi Qingchun Gao | 2011 | Atherosclerosis2011，，1： | 3 |
| 18 | Planar morphology and controlling factors of the gullies in the Yuanmou Dry-hot Valley based on field investigation显示文摘The plane form of a gully can provide a basis for evaluating the gully volume and erosion rate, acting process, and evolutionary stage. For describing the planar characteristics of a permanent gully and understanding their controlling factors, this study, utilizing a total station and GPS RTK, measured the shoulder lines and channel curves of 112 gullies in six sites of the Yuanmou Dry-hot Valley and then mapped them by Arc GIS software and calculated nine parameters. The results showed that the channel lengths range from 10.88 to 249.11 m; the widths range from 6.20 to 40.99 m; the perimeters range from 54.11 to 541.67 m; the gully areas range from 153.02 to 6,930.30 m2; the left-side areas range from 92.93 to 4,027.20 m2; and the right-side areas range from 63.65 to 3,539.77 m2. The slightly sinuous and straight gullies account for 73.21% of the total gullies; the quantity of the right skewed gullies is 8.93% greater than that of the left skewed ones based on the symmetry ratio; the shape ratios range from 1.12 to 1.40 and the morphology ratios from 0.038 to 1.294; the fractal dimension is 1.192. Gullies in different sites have diverse planar characteristics. Except for the symmetry index, which was close to a negatively skewed distribution, all of the other parameters had the characteristic of positively skewed distribution. The gully area is related to the length and width, but the gully length has a weak correlation with the width. The evolutionary stage, topographic conditions, strata, soil properties, and piping erosion played very important roles in the gully planar morphology. This study could provide useful information for controlling gully erosion and safeguarding human habitation and engineering buildings. | DENG Qingchun MIAO Fang ZHANG Bin LUO Mingliang LIU Hui LIU Xiaojiao QIN Fachao LIU Gangcai | 2015 | Journal of Arid Land2015，7，6： | 3 |
| 19 | Hydrological Impacts of Climate Change on Streamflow of Dongliao River Watershed in Jilin Province,China显示文摘The impacts of future climate change on streamflow of the Dongliao River Watershed located in Jilin Province,China have been evaluated quantitatively by using a general circulation model(HadCM3)coupled with the Soil and Water Assessment Tool(SWAT)hydrological model.The model was calibrated and validated against the historical monitored data from 2005 to 2009.The streamflow was estimated by downscaling HadCM3 outputs to the daily mean temperature and precipitation series,derived for three 30-year time slices,2020s,2050s and 2080s.Results suggest that daily mean temperature increases with a changing rate of 0.435℃per decade,and precipitation decreases with a changing rate of 0.761 mm per decade.Compared with other seasons,the precipitation in summer shows significant downward trend,while a significant upward trend in autumn.The annual streamflow demonstrates a general downward trend with a decreasing rate of 0.405 m3/s per decade.The streamflow shows significant downward and upward trends in summer and in autumn,respectively.The decreasing rate of streamflow in summer reaches 1.97 m 3 /s per decade,which contributes primarily to the decrease of streamflow.The results of this work would be of great benifit to the design of economic and social development planning in the study area. | ZHANG Lei LU Wenxi YANG Qingchun AN Yongkai LI Di GONG Lei | 2012 | Chinese Geographical Science2012，22，5： | 3 |
| 20 | Revised damage evolution equation for high cycle fatigue life prediction of aluminum alloy LC4 under uniaxial loading显示文摘The fatigue life prediction for components is a difficult task since many factors can affect the final fatigue life. Based on the damage evolution equation of Lemaitre and Desmorat, a revised two-scale damage evolution equation for high cycle fatigue is presented according to the experimental data, in which factors such as the stress amplitude and mean stress are taken into account. Then, a method is proposed to obtain the material parameters of the revised equation from the present fatigue experimental data.Finally, with the utilization of the ANSYS parametric design language(APDL) on the ANSYS platform, the coupling effect between the fatigue damage of materials and the stress distribution in structures is taken into account, and the fatigue life of specimens is predicted. The outcome shows that the numerical prediction is in accord with the experimental results, indicating that the revised two-scale damage evolution model can be well applied for the high cycle fatigue life prediction under uniaxial loading. | Zhixin ZHAN Weiping HU Miao ZHANG Qingchun MENG | 2015 | Applied Mathematics and Mechanics(English Edition)2015，36，9： | 3 |
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