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| 1 | Proteomic analysis for developing new biomarkers of hepatocellular carcinoma显示文摘AIM: To identify new markers of hepatocellular carcinoma (HCC) using a proteomic analysis. METHODS: Patients with liver cirrhosis of the three most frequent etiologies: hepatitis C virus, hepatitis B virus and alcoholic liver disease, were included in the study. The samples were analysed by 2D-electrophoresis in order to determine the differential protein expression. The proteins were separated according to the charge in immobilized pH 3-10 gradient strips and then by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins of interest were excised, digested with trypsin and the resulting peptides were separated and identified. RESULTS: Three differentially expressed apolipoproteins (Apo) were identified based on the protein profile using proteomic techniques: Apo-A1, Apo-A4 and Apo-E. Apo-A4 levels were significantly lower in HCC than in non-HCC patients regardless of etiology (P < 0.01). Multivariate logistic regression showed that Apo-A4 and Apo-A1 were the only independent factors related to HCC diagnosis (P < 0.05). The receiver operating characteristic (ROC) curve including both Apo-A4 and Apo-A1 showed an area under the ROC of 0.944 (P < 0.001), a sensitivity of 0.89 and a specificity of 0.81 for diagnosis of HCC. CONCLUSION: Apo-A4 and Apo-A1 may be used clinically as biomarkers of HCC with a high sensibility and specificity. These findings may provide additional insights into the mechanism of HCC development and progression. | Maria Pleguezuelo Laura M Lopez-Sanchez Antonio Rodriguez-Ariza Jose L Montero Javier Briceno Ruben Ciria Jordi Muntane Manuel de la Mata | 2010 | World Journal of Hepatology2010，2，3： | 3 |
| 2 | 策略将在肝移植以后减少丙肝病毒复发显示文摘 Hepatitis C virus (HCV) is a major health problem that leads to chronic hepatitis, cirrhosis and hepatocellular carcinoma, being the most frequent indication for liver transplantation in several countries. Unfortunately, HCV re-infects the liver graft almost invariably following reperfusion, with an accelerated history of recurrence, leading to 10%-30% of patients progressing to cirrhosis within 5 years of transplantation. In this sense, some groups have even advocated for not retransplanting this patients, as lower patient and graftoutcomes have been reported. However, the management of HCV recurrence is being optimized and several strategies to reduce post-transplant recurrence could improve outcomes, decrease the rate of re-transplantation and optimize the use of available grafts. Three moments may be the focus of potential actions in order to decrease the impact of viral recurrence: the pretransplant moment, the transplant environment and the post-transplant management. In the pre-transplant setting, it is not well established if reducing the pre transplant viral load affects the risk for HCV progression after transplant. Obviously, antiviral treatment can render the patient HCV RNA negative post transplant but the long-term benefit has not yet been fully established to justify the cost and clinical risk. In the transplant moment, factors as donor age, cold ischemia time, graft steatosis and ischemia/reperfusion injury may lead to a higher and more aggressive viral recurrence. After the transplant, discussion about immunosuppression and the moment to start the treatment (prophylactic, pre-emptive or once-confirmed) together with new antiviral drugs are of interest. This review aims to help clinicians have a global overview of posttransplant HCV recurrence and strategies to reduce its impact on our patients. | Ruben Ciria María Pleguezuelo Shirin Elizabeth Khorsandi Diego Davila Abid Suddle Hector Vilca-Melendez Sebastian Rufian Manuel de la Mata Javier Briceo Pedro López Cillero Nigel Heaton | 2013 | World Journal of Hepatology2013，5，5： | 2 |
| 3 | Gas-phase transformations of mercury in coal-fired power plants显示文摘 | Constance L Senior Adel F Sarofim Taofang Zeng Joseph J Helble Ruben Mamani-Paco | 2000 | Fuel Processing Technology2000，，2： | 2 |
| 4 | Dynamic characteristics of a long span seismic isolated bridge 显示文摘 | Boroschek Ruben L Moroni Mafia O Sarrazin Mauricio | 2003 | Engineering Structures2003，，25： | 1 |
| 5 | Experimental and full scale pilot plant constructed wetlands for municipat wastewaters treatment显示文摘 | Gemma A Juan M G Ruben C and Estanistao de L | 2003 | Ecological Engineering2003，21，1： | 1 |
| 6 | Regulation of purine biosynthesis by a eukaryotic-type kinase in Streptococcus agalactiae显示文摘 | Rajagopal L Vo A Silvestroni A Rubens C E | | 0，，05： | 1 |
| 7 | Regularization methods for ill- posed problems in multiple Hilbert scales 显示文摘 | Gisela M L Ruben S O | 2012 | Inverse Problems2012，28，5： | 1 |
| 8 | Purification of novel calcium binding proteins from Trypanosoma brucei: properties of 22-,24-and 38-kilodalton proteins 显示文摘 | Haghighat N G Ruben L | 1992 | Mol Biochem Parasitol1992，51，： | 1 |
| 9 | Integration of BIM and GIS:The development of the CityGML GeoBIM extension显示文摘 | Léonvan Berlo Ruben de Laat | | 0，，： | 1 |
| 10 | Employee ownership:A conceptual model of process and effects显示文摘 | Pierce J L Ruben feld SA and Morgan S | 1991 | Academy of Management Review1991，16，1： | 1 |
| 11 | Efficient Genetic Transformation System for the Ochratoxigenic Fungus Aspergillus carbonarius显示文摘 | Luiz Rodrigo I. Morioka Márcia C. Furlaneto Andréa C. Bogas Patrícia Pompermayer Rubens T. D. Duarte Maria Lúcia C. Vieira Maria Angélica Ehara Watanabe Maria Helena P. Fungaro | 2006 | Current Microbiology2006，，6： | 1 |
| 12 | Use of coaxial gas jackets to stabilize taylor cones of volatile solutions and to induce particle-to- fiber transitions 显示文摘 | Gustavo L Ruben S Raffet VO | 2004 | Advanced Materials2004，16，2： | 1 |
| 13 | In vitro propagation of the endangered plant Centaurea ultreiae : assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis显示文摘 | Ruben M Juan R Maria L G | 2010 | Plant Cell Tiss Org2010，101，1： | 1 |
| 14 | Ni-catalyzed direct carb oxylation of benzyl halides with COz 显示文摘 | THIERRY L ARKAITZ CO RUBEN M | 2013 | J Am Chem Soc2013，135，： | 1 |
| 15 | Three dimensional sonoelastography:Principles and practices 显示文摘 | Taylor L S Porter B C Rubens D J | 2000 | Phys Med Biol2000，45，6： | 1 |
| 16 | Prototype emitter for use in subsurface drip irrigation: Manufacturing, hydraulic evaluation and experimental analyses显示文摘 | Wanderley de Jesus Souza Leonor Rodrigues Sinobas Raúl Sánchez Tarlei Arriel Botrel Rubens Duarte Coelho | 2014 | Biosystems Engineering2014，，： | 1 |
| 17 | The assessment of water quality in the lower Lujan river ( Buenos Aires, Argentina ): phytoplankton and algal bioassays 显示文摘 | INES O RUBEN J L PAULA T P | 2002 | Environmental Pollution2002，120，2： | 1 |
| 18 | The value of in vitro dissolu- tion in drug development:a position paper from the AAPS in vitro release and dissolution focus group显示文摘 | TONG C RUBEN L MAO Y | 2009 | Pharm Technol2009，33，4： | 1 |
| 19 | Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence显示文摘 | rschudi C Young A S Ruben L | 1985 | Proceedings of the National Academy of Sciences1985，82，12： | 1 |
| 20 | On the nonlinear control of induction motors显示文摘 | JESUS D L M RUBEN S C | 1997 | IEEE1997，，1： | 1 |
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