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| 1 | Correlation of endoscopic disease severity with pediatric ulcerative colitis activity index score in children and young adults with ulcerative colitis显示文摘AIM To investigate of pediatric ulcerative colitis activity index(PUCAI) in ulcerative colitis correlate with mucosal inflammation and endoscopic assessment of disease activity(Mayo endoscopic score).METHODS We reviewed charts from ulcerative colitis patients who had undergone both colonoscopy over 3 years. Clinical assessment of disease severity within 35 d(either before or after) the colonoscopy were included. Patients were excluded if they had significant therapeutic interventions(such as the start of corticosteroids or immunosuppressive agents) between the colonoscopy and the clinical assessment. Mayoendoscopic score of the rectum and sigmoid were done by two gastroenterologists. Inter-observer variability in Mayo score was assessed.RESULTS We identified 99 patients(53% female, 74% pancolitis) that met inclusion criteria. The indications for colonoscopy included ongoing disease activity(62%), consideration of medication change(10%), assessment of medication efficacy(14%), and cancer screening(14%). Based on PUCAI scores, 33% of patients were in remission, 39% had mild disease, 23% had moderate disease, and 4% had severe disease. There was 'moderate-substantial' agreement between the two reviewers in assessing rectal Mayo scores(kappa = 0.54, 95%CI: 0.41-0.68). CONCLUSION Endoscopic disease severity(Mayo score) assessed by reviewing photographs of pediatric colonoscopy has moderate inter-rater reliability, and agreement was less robust in assessing patients with mild disease activity. Endoscopic disease severity generally correlates with clinical disease severity as measured by PUCAI score. However, children with inflamed colons can have significant variation in their reported clinical symptoms. Thus, assessment of both clinical symptoms and endoscopic disease severity may be required in future clinical studies. | Basavaraj Kerur Heather J Litman Julia Bender Stern Sarah Weber Jenifer R Lightdale Paul A Rufo Athos Bousvaros | 2017 | World Journal of Gastroenterology2017，23，18： | 5 |
| 2 | Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes显示文摘Traumatic brain injury(TBI)is a global cause of morbidity and mortality.Initial management and risk stratification of patients with TBI is made difficult by the relative insensitivity of screening radiographic studies as well as by the absence of a widely available,noninvasive diagnostic biomarker.In particular,a blood-based biomarker assay could provide a quick and minimally invasive process to stratify risk and guide early management strategies in patients with mild TBI(mTBI).Analysis of circulating exosomes allows the potential for rapid and specific identification of tissue injury.By applying acoustofluidic exosome separation—which uses a combination of microfluidics and acoustics to separate bioparticles based on differences in size and acoustic properties—we successfully isolated exosomes from plasma samples obtained from mice after TBI.Acoustofluidic isolation eliminated interference from other blood components,making it possible to detect exosomal biomarkers for TBI via flow cytometry.Flow cytometry analysis indicated that exosomal biomarkers for TBI increase in the first 24 h following head trauma,indicating the potential of using circulating exosomes for the rapid diagnosis of TBI.Elevated levels of TBI biomarkers were only detected in the samples separated via acoustofluidics;no changes were observed in the analysis of the raw plasma sample.This finding demonstrated the necessity of sample purification prior to exosomal biomarker analysis.Since acoustofluidic exosome separation can easily be integrated with downstream analysis methods,it shows great potential for improving early diagnosis and treatment decisions associated with TBI. | Zeyu Wang Haichen Wang Ryan Becker Joseph Rufo Shujie Yang Brian E.Mace Mengxi Wu Jun Zou Daniel T.Laskowitz Tony Jun Huang | 2021 | Microsystems & Nanoengineering2021，7，2： | 3 |
| 3 | Acoustofluidic separation of cells andparticles显示文摘Acoustofluidics,the integration of acoustics and microfluidics,is a rapidly growing research field that is addressing challenges in biology,medicine,chemistry,engineering,and physics.In particular,acoustofluidic separation of biological targets from complex fluids has proven to be a powerful tool due to the label-free,biocompatible,and contact-free nature of the technology.By carefully designing and tuning the applied acoustic field,cells and other bioparticles can be isolated with high yield,purity,and biocompatibility.Recent advances in acoustofluidics,such as the development of automated,point-of-care devices for isolating sub-micron bioparticles,address many of the limitations of conventional separation tools.More importantly,advances in the research lab are quickly being adopted to solve clinical problems.In this review article,we discuss working principles of acoustofluidic separation,compare different approaches of acoustofluidic separation,and provide a synopsis of how it is being applied in both traditional applications,such as blood component separation,cell washing,and fluorescence activated cell sorting,as well as emerging applications,including circulating tumor cell and exosome isolation. | Mengxi Wu Adem Ozcelik Joseph Rufo Zeyu Wang Rui Fang Tony Jun Huang | 2019 | Microsystems & Nanoengineering2019，5，1： | 2 |
| 4 | Increased incidence of urinary matrix metalloproteinases as predictors of disease in pedi- atric patients with inflammatory bowel disease 显示文摘 | Manfredi MA Zurakowski D Rufo PA | 2008 | Inflamm Bowel Dis2008，14，8： | 1 |
| 5 | Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation显示文摘 | Kane SV Sandborn WJ Rufo PA | | 0，，06： | 1 |
| 6 | Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation显示文摘 | Sandbom WJ Rufo PA | 2003 | Am J Gastroenterol2003，98，6： | 1 |
| 7 | Significado del líquido libre perigástrico identificado por ecoendoscopia en pacientes con cáncer gástrico显示文摘 | Alejandro Repiso Rafael López-Pardo Cristina Arribas Aurelio Aranzana Santiago Abad Rufo Rodríguez-Merlo Luis López Rafael Gómez-Rodríguez | 2012 | Gastroenterologia y Hepatologia2012，，10： | 1 |
| 8 | Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation显示文摘 | Sunanda V Kane William J Sandborn Paul A Rufo Anna Zholudev James Boone David Lyerly Michael Camilleri Stephen B Hanauer | 2003 | The American Journal of Gastroenterology2003，，： | 1 |
| 9 | Design and implemen-tation of an Ethernet-VLC interface for broadcasting transmission显示文摘 | DELGADO F QUINTANA I RUFO J | 2010 | IEEE Communications Letters2010，14，12： | 1 |
| 10 | Iron(Ⅲ) and copper (Ⅱ) catalysed cyclohexane oxidation by molecular oxygen in the presence of tert-butyl hydroperoxide显示文摘 | Schuchardt U Pereira R Rufo M | 1998 | J Mol Catal A:Chem1998，135，： | 1 |
| 11 | Design and implementa- tion of an ethemet-VLC interface for broadcast transmissions 显示文摘 | DELGADO F QUINTANA I RUFO J | 2010 | Commu- nications Letters IEEE2010，14，12： | 1 |
| 12 | Robust and sensitive monoclonal enzyme-linked immunosorbent assay for the herbicide molinate显示文摘 | Rufo C Hammock B D Gee S J | 2004 | J Agrie Food Chem2004，52，2： | 1 |
| 13 | West syndrome: Aetiology, therapeutic options, clinical course and prognostic factors显示文摘 | Arce -Portillo E Rufo -Campos M Munoz -CabeUo B | 2011 | Rev Neurol2011，52，2： | 1 |
| 14 | Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation显示文摘 | KANE S V SANDBORN W J RUFO P A | 2003 | Am J Gastroenterol2003，98，6： | 1 |
| 15 | Exposure to extremely low frequency magnetic fields in an urban area显示文摘 | Paniagua J M Jime'nez A Rufo M | 2007 | Radiation&Environmental Biophysics2007，46，1： | 1 |
| 16 | Modeled mierogravity stimulates osteoelastogenesis and bone resorption by increasing osteoblast RANKIZOPG ratio 显示文摘 | Rueei N Rufo A Alamanou M | 2007 | J Cell Bioehem2007，100，46： | 1 |
| 17 | β-Arrestin 2 regulates RANKL and ephrins gene expression in response to bone remodeling in mice 显示文摘 | Pierroz DD Rufo A Bianchi EN | 2009 | J Bone Miner Res2009，24，5： | 1 |
| 18 | Challenges and progress in pediatric inflammatory bowel disease显示文摘 | Rufo PA Bousvaros A | 2007 | Curt Opin Gastroenterol2007，23，4： | 1 |
| 19 | Driving cost factors in a-dult outpatientswith refractory epilepsy:a daily clinical practice in clinics of neurology in spain显示文摘 | Pena P Sancho J Rufo M | 2009 | Epilepsy Res2009，83，23： | 1 |
| 20 | Iron (Ⅱ) and copper(Ⅱ)catalysed cyclohexane oxidation by molecular oxygen in the presence of tert-butyl hydroperoxide显示文摘 | Schuchardt U Pereira R Rufo M | 1998 | J Mol Catal A:Chem1998，135，： | 1 |
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