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| 1 | Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prognosis in esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma(ESCC)is a poor-prognosis cancer type with limited understanding of its molecular etiology.Using 508 ESCC genomes,we identified five novel significantly mutated genes and uncovered mutational signature clusters associated with metastasis and patients’outcomes.Several functional assays implicated that NFE2L2 may act as a tumor suppressor in ESCC and that mutations in NFE2L2 probably impaired its tumor-suppressive function,or even conferred oncogenic activities.Additionally,we found that the NFE2L2 mutations were significantly associated with worse prognosis of ESCC.We also identified potential noncoding driver mutations including hotspot mutations in the promoter region of SLC35E2 that were correlated with worse survival.Approximately 5.9%and 15.2%of patients had high tumor mutation burden or actionable mutations,respectively,and may benefit from immunotherapy or targeted therapies.We found clinically relevant coding and noncoding genomic alterations and revealed three major subtypes that robustly predicted patients’outcomes.Collectively,we report the largest dataset of genomic profiling of ESCC useful for developing ESCC-specific biomarkers for diagnosis and treatment. | Yongping Cui Hongyan Chen Ruibin Xi Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qimin Zhan Yanhong Li Zhihua Liu-Show | 2020 | Cell Research2020，30，10： | 19 |
| 2 | Salt tolerance of transgenic rice (Oryza sativa L.) with mtlD gene and gutD gene显示文摘Southern blot analysis indicated that mtID gene (encoding mannitol-1 -phosphate dehydrogenase) and gutD gene (encoding glucitol-6-phosphate dehydrogenase) had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404(pBIGM). The expression of the above two genes in transgenic rice plants was demonstrated by Northern blot analysis and enzymatic activity assay. Analysis of sugar alcohol showed that transgenic rice plants could produce and accumulate mannitol and sorbitol. The salt tolerance of transgenic plants was much higher than that of their controls. | WANG Huizhong HUANG Danian LU Ruifang LIU Junjun QIAN Qian PENG Xuexian | 2000 | Chinese Science Bulletin2000，45，18： | 5 |
| 3 | Olivine versus peridotite during serpentinization:Gas formation显示文摘The dependence of starting materials and their initial grain sizes on the formation of gases(H2, CH4, C2H6 and C3H8) during serpentinization was investigated by conducting hydrothermal experiments at 311°C and 3 kbar on olivine and peridotite with initial grain sizes ranging from <30 to 177 ?m. Hydrocarbons(CH4, C2H6 and C3H8) were produced from reaction between dissolved CO2 in the starting fluids and H2 formed during serpentinization, which were analyzed by Gas Chromatography. It was found that olivine serpentinization produced much less H2 and CH4 compared with those after peridotite alteration, while their C2H6 and C3H8 were identical. For example, for olivine with initial grain sizes of <30 ?m, the amounts of H2 and CH4 were 79.6 mmol/kg and 460 ?mol/kg after 27 days, respectively. By contrast, the quantities of H2 and CH4 produced in experiment on peridotite with the same run duration were much larger, 119 mmol/kg and 1300 ?mol/kg, respectively. This indicates that spinel and pyroxene in peridotite may increase the amounts of H2 and hydrocarbons, possibly due to the catalytic effect of aluminum released by spinel and pyroxene during serpentinization. Moreover, the production of H2 and hydrocarbons is negatively correlated with initial grain sizes of the starting material, with smaller amounts of H2 and hydrocarbons for larger initial grain sizes, indicating that the kinetics of serpentinization influences the formation of H2 and hydrocarbons, possibly because of the lack of catalytic minerals for the starting material with larger grain sizes. This study suggests that olivine cannot completely represent peridotite during serpentinization, and that H2 and hydrocarbons in hydrothermal fields near the mid-ocean ridge may be produced in a very long period of serpentinization or the presence of catalytic minerals due to large grain sizes of ultramafic rocks. | HUANG RuiFang SUN WeiDong DING Xing LIU JinZhong PENG ShaoBang | 2015 | Science China Earth Sciences2015，58，12： | 5 |
| 4 | The production of iron oxide during peridotite serpentinization:Influence of pyroxene显示文摘Serpentinization produces molecular hydrogen(H2)that can support communities of microorganisms in hydrothermal fields;H2 results from the oxidation of ferrous iron in olivine and pyroxene into ferric iron,and consequently iron oxide(magnetite or hematite)forms.However,the mechanisms that control H2 and iron oxide formation are poorly constrained.In this study,we performed serpentinization experiments at 311℃ and 3.0 kbar on olivine(with <5% pyroxene),orthopyroxene,and peridotite.The results show that serpentine and iron oxide formed when olivine and orthopyroxene individually reacted with a saline starting solution.Olivine-derived serpentine had a significantly lower FeO content(6.57±1.30 wt.%)than primary olivine(9.86 wt.%),whereas orthopyroxene-derived serpentine had a comparable FeO content(6.26±0.58 wt.%)to that of primary orthopyroxene(6.24 wt.%).In experiments on peridotite,olivine was replaced by serpentine and iron oxide.However,pyroxene transformed solely to serpentine.After 20 days,olivine-derived serpentine had a FeO content of 8.18±1.56 wt.%,which was significantly higher than that of serpentine produced in olivine-only experiments.By contrast,serpentine after orthopyroxene had a slightly higher FeO content(6.53±1.01 wt.%)than primary orthopyroxene.Clinopyroxene-derived serpentine contained a significantly higher FeO content than its parent mineral.After 120 days,the FeO content of olivine-derived serpentine decreased significantly(5.71±0.35 wt.%),whereas the FeO content of orthopyroxene-derived serpentine increased(6.85±0.63 wt.%)over the same period.This suggests that iron oxide preferentially formed after olivine serpentinization.Pyroxene in peridotite gained some Fe from olivine during the serpentinization process,which may have led to a decrease in iron oxide production.The correlation between FeO content and SiO_2 or AI_2 O_3 content in olivine-and orthopyroxene-derived serpentine indicates that aluminum and silica greatly control the production of iron oxide.Based on our results and data from natural serpentinites reported by other workers,we propose that aluminum may be more influential at the early stages of peridotite serpentinization when the production of iron oxide is very low,whereas silica may have a greater control on iron oxide production during the late stages instead. | Ruifang Huang Chiou-Ting Lin Weidong Sun Xing Ding Wenhuan Zhan Jihao Zhu | 2017 | Geoscience Frontiers2017，8，6： | 3 |
| 5 | Effects of salinity on embryonic and larval development of Chinese mitten crab Eriocheir sinensis(Decapoda: Brachyura) and salinity-induced physiological changes显示文摘To investigate the effects of salinity on early development of Chinese mitten crab ( Eriocheir sinensis ), and the salinity tolerance mechanism of embryos, different developmental stages of embryos (gastrula, eyespot and pre-hatching stage), and hatched stage I zoea and megalopa, were exposed to a range of salinities (1, 5, 10, 15 (control), 20, 25, 30, 35 and 40). Hatching, survival and molting were monitored. Effects of 24-hour hypersaline (35) and hyposaline (1) stress on egg diameter, water content, Na +/K +-ATPase (NKA) activity, and crustacean hyperglycemic hormone (CHH) gene mRNA expression in embryos and megalopa, are reported. Embryos are more tolerant of low (≤ 5) than high (≥25) salinities, with optimum ranges for gastrula and pre-hatching stage embryos being 5-20, and for eyespot embryo and stage I zoea, 10-20. Most megalopa can molt to the first juvenile instar by day 5 at salinities between 1 and 40, whereas molting of megalopa stages was delayed at 40. Hypersaline conditions resulted in a loss of moisture, reduction of egg volume, and a signifi cant increase in NKA activity and CHH mRNA expression at some developmental stages. Hyposaline conditions did not affect moisture content or egg volume, but resulted in decreased NKA activity and CHH mRNA expression in embryos. For megalopa stages, NKA activity was significantly upregulated following both hypo- and hypersaline stress. Our results suggest high salinity will inhibit development and hatching of E. sinensis embryos, and low salinity will affect the survival of their stage I zoea. Increased NKA activity in embryos and megalopa stages might indicate a hyporegulation response under hypersaline conditions. These findings provide useful information for spawning ground protection of indigenous E . sinensis and enrich the knowledge of embryonic tolerance mechanisms of hyperregulating crustaceans following osmotic stress. | WANG Ruifang HUANG Xiaorong WANG Haihua LU Jianxue SHI Xiaotao FENG Guangpeng ZHUANG Ping | 2019 | Journal of Oceanology and Limnology2019，37，5： | 2 |
| 6 | Transcriptome and Metabolome Analyses of Sea Cucumbers Apostichopus japonicus in Southern China During the Summer Aestivation Period显示文摘Aestivation is a common strategy of sea cucumbers(Apostichopus japonicus)in response to high-temperature conditions.Previous studies have individually investigated the immune and physiological alterations at the aestivation stage.However,these studies have not evaluated the relationship between immunity and physiology.In this study,we explored the transcriptome and metabolome of A.japonicus during the aestivation stage to study the relationship.The transcriptome analysis of dormant(aestivation)and revived A.japonicus generated 2368 differentially expressed genes,including 927 downregulated genes and 1441 upregulated genes.Based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses,the downregulated genes in the dormant group were found to be involved in DNA replication,RNA metabolic process,and protein metabolism,which results in the inhibition of motility,skeletal development,neural activity,cell proliferation,and development of A.japonicus.In contrast,the upregulated genes were found to be associated with fatty acid metabolism,carbohydrate hydrolysis,and phagocytosis.In the metabolome analysis,the downregulated metabolites were found to be associated with fatty acid metabolism,starch and sucrose metabolism,and TCA cycle.This indicates that dormant sea cucumbers consume reserved carbohydrates and fatty acids to maintain low levels of energy supply.The protein-protein interaction network analysis further revealed that carbohydrate hydrolysis promoted phagocytosis activity in the dormant group.This study provides new insights into potential molecular mechanisms of sea cucumber survival in high-temperature conditions,which is critical in aquaculture of sea cucumbers. | YANG Qiuhua ZHANG Xusheng LU Zhen HUANG Ruifang TRAN Ngoc Tuan WU Jianshao YANG Fuyuan GE Hui ZHONG Chenhui SUN Qian ZHOU Chen LIN Qi | 2021 | Journal of Ocean University of China2021，20，1： | 2 |
| 7 | QTL mapping of quality traits in peanut using whole-genome resequencing显示文摘Oil and protein content and fatty acid composition are quality traits in peanut.Elucidating the genetic mechanisms underlying these traits may help researchers to obtain improved cultivars by molecular breeding.Whole-genome resequencing of a recombinant inbred population of 318 lines was performed to construct a high-density linkage map and identify QTL for peanut quality.The map,containing 4561 bin markers,covered 2032 c M with a mean marker density of 0.45 c M.A total of 110 QTL for oil and protein content,and fatty acid composition were mapped on the 18 peanut chromosomes.The QTL q A05.1 was detected in four environments and showed a major phenotypic effect on the contents of oil,protein,and six fatty acids.The genomic region spanned by q A05.1,corresponding to a physical interval of approximately 1.5 Mb,contains two SNPs polymorphic between the parents that could cause missense mutations.The two SNP sites were employed as KASP markers and validated using lines with extremely high and low oil contents.These sites may be useful in the marker-assisted breeding of peanut cultivars with high oil contents. | Ziqi Sun Feiyan Qi Hua Liu Li Qin Jing Xu Lei Shi Zhongxin Zhang Lijuan Miao Bingyan Huang Wenzhao Dong Xiao Wang Mengdi Tian Jingjing Feng Ruifang Zhao Xinyou Zhang Zheng Zheng | 2022 | The Crop Journal2022，10，1： | 2 |
| 8 | Contemporary medicinal chemistry strategies for the discovery and optimization of influenza inhibitors targeting vRNP constituent proteins显示文摘Influenza is an acute respiratory infectious disease caused by the influenza virus,affecting people globally and causing significant social and economic losses.Due to the inevitable limitations of vaccines and approved drugs,there is an urgent need to discover new anti-influenza drugs with different mechanisms.The viral ribonucleoprotein complex(vRNP)plays an essential role in the life cycle of influenza viruses,representing an attractive target for drug design.In recent years,the functional area of constituent proteins in vRNP are widely used as targets for drug discovery,especially the PA endonuclease active site,the RNA-binding site of PB1,the cap-binding site of PB2 and the nuclear export signal of NP protein.Encouragingly,the PA inhibitor baloxavir has been marketed in Japan and the United States,and several drug candidates have also entered clinical trials,such as favipiravir.This article reviews the compositions and functions of the influenza virus vRNP and the research progress on vRNP inhibitors,and discusses the representative drug discovery and optimization strategies pursued. | Lingxin Hou Ying Zhang Han Ju Srinivasulu Cherukupalli Ruifang Jia Jian Zhang Bing Huang Arianna Loregian Xinyong Liu Peng Zhan | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 2 |
| 9 | Dynamic changes in O-GlcNAcylation regulate osteoclast differentiation and bone loss via nucleoporin 153显示文摘Bone mass is maintained by the balance between osteoclast-induced bone resorption and osteoblast-triggered bone formation.In inflammatory arthritis such as rheumatoid arthritis(RA),however,increased osteoclast differentiation and activity skew this balance resulting in progressive bone loss.O-GlcNAcylation is a posttranslational modification with attachment of a single O-linkedβ-D-N-acetylglucosamine(O-GlcNAc)residue to serine or threonine residues of target proteins.Although O-GlcNAcylation is one of the most common protein modifications,its role in bone homeostasis has not been systematically investigated.We demonstrate that dynamic changes in O-GlcNAcylation are required for osteoclastogenesis.Increased O-GlcNAcylation promotes osteoclast differentiation during the early stages,whereas its downregulation is required for osteoclast maturation.At the molecular level,O-GlcNAcylation affects several pathways including oxidative phosphorylation and cell-cell fusion.TNFαfosters the dynamic regulation of O-GlcNAcylation to promote osteoclastogenesis in inflammatory arthritis.Targeted pharmaceutical or genetic inhibition of O-GlcNAc transferase(OGT)or O-GlcNAcase(OGA)arrests osteoclast differentiation during early stages of differentiation and during later maturation,respectively,and ameliorates bone loss in experimental arthritis.Knockdown of NUP153,an O-GlcNAcylation target,has similar effects as OGT inhibition and inhibits osteoclastogenesis.These findings highlight an important role of O-GlcNAcylation in osteoclastogenesis and may offer the potential to therapeutically interfere with pathologic bone resorption. | Yi-Nan Li Chih-Wei Chen Thuong Trinh-Minh Honglin Zhu Alexandru-Emil Matei Andrea-Hermina Györfi Frederic Kuwert Philipp Hubel Xiao Ding Cuong Tran Manh Xiaohan Xu Christoph Liebel Vladyslav Fedorchenko Ruifang Liang Kaiyue Huang Jens Pfannstiel Min-Chuan Huang Neng-Yu Lin Andreas Ramming Georg Schett Jörg H.W.Distler | 2022 | Bone Research2022，10，4： | 1 |
| 10 | General Growth of Carbon Nanotubes for Cerium Redox Reactions in High-Efficiency Redox Flow Batteries显示文摘Carbon nanotubes(CNTs)possess remarkable mechanical,electrical,thermal,and optical properties that predestine them for numerous potential applications.The conventional chemical vapor deposition(CVD)route for the production of CNTs,however,suffers from costly and complex issues.Herein,we demonstrate a general and high-yield strategy to grow nitrogendoped CNTs(NCNTs)on three-dimensional(3D)graphite felt(GF)substrates,through a direct thermal pyrolysis process simply using a common tube furnace,instead of the costly and complex CVD method.Specifically,the NCNTs-decorated GF(NCNT-GF)electrode possesses enhanced electrocatalytic performance towards cerium redox reactions,mainly due to the catalytic effect of N atoms doped into NCNTs,and ingenious and hierarchical 3D architecture of the NCNT-GF.As a result,the cell with the NCNT-GF serving as a positive electrode shows the improved energy efficiency with increases of about 53.4%and 43.8%over the pristine GF and the acidly treated GF at a high charge/discharge rate of 30 mA cm^(-2),respectively.Moreover,the as-prepared NCNT catalyst-enhanced electrode is found to be highly robust and should enable a long-term cycle without detectable efficiency loss after 500 cycles.The viable synthetic strategy reported in this study will contribute to the further development of more active heteroatom-doped CNTs for redox flow batteries. | Zhaolin Na Ruifang Yao Qing Yan Xudong Sun Gang Huang | 2019 | Research2019，，1： | 1 |
| 11 | In vivo biocompatibility and biodegradation of a Mg-15%Ca 3 (PO 4 ) 2 composite as an implant material显示文摘 | Kun Yu Liangjian Chen Jun Zhao Ruifang Wang Yilong Dai Qiao Huang | 2013 | Materials Letters2013，，： | 1 |
| 12 | Ran binding protein 9(RanBPM) binds IFN-λR1 in the IFN-λsignaling pathway显示文摘Like the type I interferons(IFNs),the recently discovered cytokine IFN-λ displays antiviral,antiproliferative,and proapoptotic activities,mediated by a heterodimeric IFN-λ receptor complex composed of a unique IFN-λR1 chain and the IL-10R2 chain.However,the molecular mechanism of the IFN-λ-regulated pathway remains unclear.In this study,we newly identified RAN-binding protein M(RanBPM) as a binding partner of IFN-λR1.The interaction between RanBPM and IFN-λRl was identified with a glutathione S-transferase pull-down assay and coimmunoprecipitation experiments.IFN-λ1 stimulates this interaction and affects the cellular distribution of RanBPM.However,the interaction between RanBPM and IFN-λR1 does not correlate with their conserved TRAF6-binding sites.Furthermore,we also found that RanBPM is a scaffolding protein with a modulatory function that regulates the activities of IFN-stimulated response elements.Therefore,RanBPM plays a novel role in the IFN-λ-regulated signaling pathway. | Junwen Zhang XiaojieCong Jiajie Zhaoqiao Xia Yang Meng Li Hong Chen Ruifang Mi Guishan Jin Fusheng Liu Bing-Ren Huang | 2017 | Science China(Life Sciences)2017，60，9： | 1 |
| 13 | Panicle-3D: A low-cost 3D-modeling method for rice panicles based on deep learning, shape from silhouette, and supervoxel clustering显示文摘Self-occlusions are common in rice canopy images and strongly influence the calculation accuracies of panicle traits. Such interference can be largely eliminated if panicles are phenotyped at the 3 D level.Research on 3 D panicle phenotyping has been limited. Given that existing 3 D modeling techniques do not focus on specified parts of a target object, an efficient method for panicle modeling of large numbers of rice plants is lacking. This paper presents an automatic and nondestructive method for 3 D panicle modeling. The proposed method integrates shoot rice reconstruction with shape from silhouette, 2 D panicle segmentation with a deep convolutional neural network, and 3 D panicle segmentation with ray tracing and supervoxel clustering. A multiview imaging system was built to acquire image sequences of rice canopies with an efficiency of approximately 4 min per rice plant. The execution time of panicle modeling per rice plant using 90 images was approximately 26 min. The outputs of the algorithm for a single rice plant are a shoot rice model, surface shoot rice model, panicle model, and surface panicle model, all represented by a list of spatial coordinates. The efficiency and performance were evaluated and compared with the classical structure-from-motion algorithm. The results demonstrated that the proposed method is well qualified to recover the 3 D shapes of rice panicles from multiview images and is readily adaptable to rice plants of diverse accessions and growth stages. The proposed algorithm is superior to the structure-from-motion method in terms of texture preservation and computational efficiency. The sample images and implementation of the algorithm are available online. This automatic, cost-efficient, and nondestructive method of 3 D panicle modeling may be applied to high-throughput 3 D phenotyping of large rice populations. | Dan Wu Lejun Yu Junli Ye Ruifang Zhai Lingfeng Duan Lingbo Liu Nai Wu Zedong Geng Jingbo Fu Chenglong Huang Shangbin Chen Qian Liu Wanneng Yang | 2022 | The Crop Journal2022，10，5： | 1 |
| 14 | Differential diagnosis of high-grade squamous intraepithelial lesions and benign atrophy in older women using p16 immunocytochemistry显示文摘Background For cervical cancer screening,routine cytology has a high specificity but a lower sensitivity.In older women,atrophy,which may mimic HSIL,presents a diagnostic challenge.p16 is a widely used biomarker for histological diagnosis of HSIL.Our objective was to evaluate PathCIN®p16INK4a immunocytology in identification of high grade dysplasia vs.benign atrophy.Methods As part of a multi-center screening program,3351 women were co-tested by p16 immunocytology.Among women referred for colposcopy on basis of cytology and high-risk HPV status,those with atrophy were older than the population screened(52 vs.43 years).Cases from older women with atrophy(n=116)and controls without atrophy(n=47)were identified by re-examination of Pap smears.The detection of CIN2+was compared for p16,cytology and HR-HPV results.Results The sensitivity of routine cytology(≥LSIL)was much lower for cases with atrophy(17%)than non-atrophic cases(75%).The sensitivity of p16 immunocytology and of HR-HPV testing was high(88%–100%)both with and without atrophy.The specificity of routine cytology(≥LSIL)was higher for cases with atrophy(79%)than non-atrophic cases(38%).The specificity of p16 immunocytology was high(88–95%)and the specificity of HR-HPV testing was low(31%–33%)both with and without atrophy.Combining p16 with HPV testing and/or routine cytology had no benefit,as compared to p16 staining alone.Conclusions p16 immunocytology compares favorably with routine cytology and HPV testing in the differential diagnosis of HSIL and benign atrophy.It is more sensitive than cytology for atrophic specimens,and is more specific than HPV testing.p16 immunocytology may decrease the need for colposcopy referrals and could be a useful tool for early detection of cervical cancer in peri-and post-menopausal women,who are more likely to have HSIL coexisting with atrophy. | Peisha Yan Hui Du Chun Wang Fangbin Song Xia Huang Yingjie Luo Ruifang Wu | 2021 | Gynecology and Obstetrics Clinical Medicine2021，1，1： | 0 |
| 15 | New N-acetyl-L-cysteine derivatives synthesized by the degradation of proanthocyanidins as high biological activity antioxidants显示文摘A novel degradation method was investigated to synthesize highly biologically active flavan-3-ol derivatives in the presence of N-acetyl-L-cysteine(NAC)as a nucleophile under acidic conditions for polymerized proanthocyanidins degradation.The reaction conditions were optimized by the combination of single-factor test and central composite experimental design(CCD).Grape seed proanthocyanidins were reacted with NAC at a ratio of 1:3 with 0.3 M methanolic HC1,a temperature of 55°C,and a reaction time of 50 mins.Most of the degradation products were separated and prepared by one-step high-speed countercurrent chromatography(HSCCC)and preparative high-performance liquid chromatography(prep-HPLC).Three monomeric pro anthocyanidins and four new N-acetyl-L-cysteine derivatives were isolated from degradation products with total degradation yield of 55.44%and high purity over 95%.Furthermore,the neuroprotective abilities of these compounds to H2O2-treated PC-12 neuroblastoma cells were evaluated.NAC derivatives showed better antioxidant activity than their corresponding underivatized monomers and NAC,indicating that they had a better performance in protecting PC-12 cells from oxidative stress damage. | Yuxuan Wang Ruifang Bai Siqi Luo Meng Huang Yan Cui | 2021 | Journal of Polyphenols2021，3，1： | 0 |
| 16 | Early warning of emerging infectious diseases based on multimodal data显示文摘The coronavirus disease 2019 (COVID-19) pandemic has dramatically increased the awareness of emerging infectious diseases. The advancement of multiomics analysis technology has resulted in the development of several databases containing virus information. Several scientists have integrated existing data on viruses to construct phylogenetic trees and predict virus mutation and transmission in different ways, providing prospective technical support for epidemic prevention and control. This review summarized the databases of known emerging infectious viruses and techniques focusing on virus variant forecasting and early warning. It focuses on the multi-dimensional information integration and database construction of emerging infectious viruses, virus mutation spectrum construction and variant forecast model, analysis of the affinity between mutation antigen and the receptor, propagation model of virus dynamic evolution, and monitoring and early warning for variants. As people have suffered from COVID-19 and repeated flu outbreaks, we focused on the research results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses. This review comprehensively viewed the latest virus research and provided a reference for future virus prevention and control research. | Haotian Ren Yunchao Ling Ruifang Cao Zhen Wang Yixue Li Tao Huang | 2023 | Biosafety and Health2023，5，4： | 0 |
| 17 | Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers显示文摘Intensive selective breeding for high growth rate and body weight cause excess abdominal fat in broilers.Gut microbiota and folic acid were reported to regulate lipid metabolism.A total of 210 one-day-old broilers were divided into the control(folic acid at 1.3 mg/kg)and folic acid groups(folic acid at 13 mg/kg)to illustrate the effects of folic acid on growth performance,abdominal fat deposition,and gut microbiota,and the experiment lasted 28 d.Results revealed that dietary folic acid addition decreased abdominal fat percentage(P<0.05)and down-regulated genes expression related to cell proliferation and differentiation in abdominal fat including IGF1,EGF,C/EBPα,PPARγ,PLIN1,FABP4 and PCNA(P<0.05).Folic acid addition decreased caecal Firmicutes-to-Bacteroidetes ratio(P<0.01)and increased the proportions of Alistipes,Oscillospira,Ruminococcus,Clostridium,Dehalobacterium and Parabacteroides(P<0.05).Caecal acetic acid,and propionic acid contents were found to be higher under folic acid treatment(P<0.05),which were negatively related to genes expression associated with adipocyte proliferation and differentiation(P<0.05).Ruminococcus was positively correlated with caecal acetic acid content,and the same phenomenon was detected between propionic acid and Oscillospira and Ruminococcus(P<0.05).Acetic acid and Oscillospira were identified to be negatively associated with abdominal fat percentage(P<0.05).In conclusion,our data demonstrated that dietary supplementation of folic acid reduced fat deposition in broilers by inhibiting abdominal adipocyte proliferation and differentiation,which might be mediated by changes in gut microbiota and short chain fatty acid production. | Yanli Liu Jiantao Yang Xiaoying Liu Rui Liu Yibin Wang Xinhuo Huang Yingge Li Ruifang Liu Xiaojun Yang | 2023 | Animal Nutrition2023，，1： | 0 |
| 18 | The influence of silica on reaction rates and molecular hydrogen(H_(2))generation during olivine hydrothermal alteration显示文摘Hydrothermal alteration of olivine greatly influences geodynamics and the recycling of volatiles(such as water and carbon)in subduction zones.Silica is an important component of geological fluids,and its influence on the hydrothermal alteration of olivine remains poorly constrained.In this study,we performed experiments at 300–515℃ and 3.0 kbar(1 bar=10^(5)Pa)by reacting well homogenized mixtures of olivine and silica powders with saline solutions(0.5 mol L^(−1)NaCl).Silica greatly influences the reaction pathways,reaction rates,and molecular hydrogen(H_(2))formation during olivine hydrothermal alteration.In experiments at 300℃ and 3.0 kbar with mixtures of olivine and 10 wt%silica,olivine was replaced by serpentine and talc.The proportions of serpentine and talc were determined according to standard curves based on infrared spectroscopy analyses.Around 6.5%serpentine and 12%talc were produced after an experimental duration of 7 days,which had no change after a longer period(14 days).Compared to the kinetics in silica-free systems,the rates of olivine hydrothermal alteration in experiments with 10 wt%silica are much lower.The overall reaction is:4.5Forsterite+5.5SiO_(2),aq+4H_(2)O=Serpentine+2Talc.With the addition of more silica(20 wt%and 40 wt%),olivine was transformed into talc.The rates of reaction were much faster,e.g.,for experiments with olivine and 20 wt%silica,43%of talc was produced after 14 days,which increased to 77%for experiments with 40 wt%silica over the same period.The overall reaction is:3Forsterite+5SiO_(2),aq+2H_(2)O=2Talc.In experiments at 400–505℃ and 3.0 kbar,the promoting effect of silica on olivine hydrothermal alteration was also observed,which is closely associated with a decrease in Gibbs free energies of olivine hydrothermal alteration.At 300℃ and 3.0 kbar,silica decreased H_(2)formed during olivine hydrothermal alteration by around an order of magnitude,resulting in an increase in oxygen fugacity.Based on measured H_(2),we calibrated oxygen fugacities,ranging from 0.96 to 3.41 log units below FMQ(fayalite-magnetitequartz buffer assemblage).This study suggests that the infiltration of SiO_(2)-bearing fluids into peridotites greatly influences redox conditions and the rates of olivine hydrothermal alteration. | Ruifang HUANG Weidong SUN Xing DING Yusheng ZHAO Yibing LI Xiuqi SHANG | 2024 | Science China Earth Sciences2024，67，1： | 0 |
| 19 | Author Correction:Whole-genome sequencing of 508 patients identifies key molecular features associated with poor prog nosis in esophageal squamous cell carcinoma显示文摘Correction to:Cell Research http://gffzzd3cc09b8251d45dfswxb66oc955ov6fun.ffgz.tsg.suse.edu.cn/10.1038/s41422-020-0333-6,published online 12 May 2020 In the initial published version of this article,there was an error in the Supplementary information,Table S1.Row 463 and row 80 have the same sample id BDESCC-01-838.After checking the original id of these two samples,we found that they were actually two different samples.This error was caused by Excel random numbering when we assigned the external id to each sample.Thus,we re-assigned a new id BDESCC-01-839 to the row 4P3 sample to fix this issue. | Yongping Cui Hongyan Chen Ruibin Xie Heyang Cui Yahui Zhao Enwei Xu Ting Yan Xiaomei Lu Furong Huang Pengzhou Kong Yang Li Xiaolin Zhu Jiawei Wang Wenjie Zhu Jie Wang Yanchun Ma Yong Zhou Shiping Guo Ling Zhang Yiqian Liu Bin Wang Yanfeng Xi Ruifang Sun Xiao Yu Yuanfang Zhai Fang Wang Jian Yang Bin Yang Caixia Cheng Jing Liu Bin Song Hongyi Li Yi Wang Yingchun Zhang Xiaolong Cheng Qi min Zhan Yanhong Li Zhihua Liu | 2022 | Cell Research2022，32，4： | 0 |
| 20 | Melatonin activates the OsbZIP79–OsABI5 module that orchestrates nitrogen and ROS homeostasis to alleviate nitrogen-limitation stress in rice显示文摘Melatonin(Mel)has previously been reported to effectively alleviate nitrogen-limitation(N-L)stress and thus increase nitrogen-use efficiency(NUE)in several plants,but the underlying mechanism remains obscure.Here,we revealed that OsbZIP79(BASIC LEUCINE ZIPPER 79)is transcriptionally activated under N-L conditions,and its expression is further enhanced by exogenous Mel.By the combined use of omics,genetics,and biological techniques,we revealed that the OsbZIP79–OsABI5(ABSCISIC ACID INSENSITIVE 5)module stimulated regulation of reactive oxygen species(ROS)homeostasis and the uptake and metabolismof nitrogen under conditions of indoor nitrogen limitation(1/16 normal level).OsbZIP79 activated the transcription of OsABI5,and OsABI5 then bound to the promoters of target genes,including genes involved in ROS homeostasis and nitrogen metabolism,activating their transcription.This module was also indispensable for upregulation of several other genes involved in abscisic acid catabolism,nitrogen uptake,and assimilation under N-L and Mel treatment,although these genes were not directly transactivated by OsABI5.Field experiments demonstrated that Mel significantly improved rice growth under low nitrogen(L-N,half the normal level)by the same mechanism revealed in the nitrogen-limitation study.Mel application produced a 28.6%yield increase under L-N and thus similar increases in NUE.Also,two OsbZIP79-overexpression lines grown in L-N field plots had significantly higher NUE(+13.7%and+21.2%)than their wild types.Together,our data show that an OsbZIP79–OsABI5 module regulates the rice response to N insufficiency(N limitation or low N),which is important for increasing NUE in rice production. | Meng Jiang Yue Song Ruifang Yang Chenfan Zheng Yunchao Zheng Huali Zhang Shan Li Yuanyuan Tan Jianzhong Huang Qingyao Shu Ruiqing Li | 2023 | Plant Communications2023，4，6： | 0 |
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