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| 1 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 2 | Genome-wide Characterization of cis-acting Elements in the Promoters of Key Carotenoid Pathway Genes from the Main Species of Genus Citrus显示文摘Carotenoids are indispensable for both human health and plant survival.Citrus,is one of the fruit crops richest in carotenoid compounds,with approximately 115 kinds of carotenoids;tremendous diversity in carotenoids composition and concentration exists among various species,showing different colors from nearly white to crimson.The carotenoid biosynthetic pathway and the key carotenogenic genes have been identified in citrus;however,the underlying regulatory mechanisms remain unclear.In this study,among the main species of genus Citrus(primitive,wild,and cultivated),we detected carotenoids in flavedo using High-Performance Liquid Chromatography,and analyzed variations in cis-acting elements in the promoters of key carotenoid pathway genes.Intriguingly,both carotenoid composition and content were generally increased during the evolution of citrus,and the corresponding variations in the promoters were identified,including the gain or loss of critical environmental stress-responsive elements and hormone-responsive elements,which are closely associated with carotenoid enhancement.In addition,pummelo has the most heat-responsive elements,but the Mangshan mandarin does not have this element in the promoters of PSY,which is highly related to their geographical origin and indicate that temperature is a critical environmental signal influencing carotenoid accumulation.Moreover,the abscisic acid-responsive motif was rich in almost all the seven species,but the ethylene-responsive motif was deficient,which demystified the unique phytohormone regulation mechanism of carotenoid accumulation in citrus.Overall,our study provides new insights into the molecular regulatory mechanism of carotenoid enhancement in the evolution of citrus,which can facilitate breeding and cultivation efforts to improve the nutritional quality and esthetic value in citrus and hopefully other fruit crops. | Kaijie Zhu Qingjiang Wu Yue Huang Junli Ye Qiang Xu Xiuxin Deng | 2020 | Horticultural Plant Journal2020，6，6： | 8 |
| 3 | Mechanism of vertical crack formation in Yb_(2)SiO_(5) coatings deposited via plasma spray-physical vapor deposition显示文摘Plasma spray-physical vapor deposition(i.e.,PS-PVD)is a promising method for obtaining advanced environmental barrier coatings(EBCs).The EBCs must meet some requirements in the application,in which the thermal cycle performance affects the service lifetime.The preparation of artificial vertical cracks in Yb_(2)SiO_(5) coatings is an effective approach for meeting the requirements above because vertical cracks provide a strain tolerance.To clarify the formation mechanism of vertical cracks during the PSPVD,the effects of coating thickness and substrate temperature on the formation of vertical cracks were investigated.In addition,the interactions of spray powder and plasma flame during coating deposition were also characterized by optical spectroscopy.It is indicated that vertical cracks are formed due to a thermal expansion mismatch between Yb_(2)SiO_(5) and mullite coating,transient cooling after deposition and the nucleation of evaporated Yb_(2)SiO_(5) as well. | Xiaofeng Zhang Chao Wang Ruijun Ye Chunming Deng Xinghua Liang Ziqian Deng Shaopeng Niu Jinbing Song Guo Liu Min Liu Kesong Zhou Jian Lu Junli Feng | 2020 | Journal of Materiomics2020，6，1： | 6 |
| 4 | Microarray Expression Profiling of Postharvest Ponkan Mandarin (Citrus reticulata) Fruit under Cold Storage Reveals Regulatory Gene Candidates and Implications on Soluble Sugars Metabolism显示文摘Low temperature storage is widely applied to maintain citrus postharvest fruit quality.In this study,the transcriptional and metabolic changes in the pulp tissue of Citrus reticulata Blanco cv.'Ponkan' were studied for three successive months under cold storage by Affymetrix Citrus GeneChip and gas chromatography,respectively.As many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model.The statistical analysis of gene ontology revealed that defense/stress-related genes were induced quickly,while autophagy-related genes were overrepresented in the late sampling stages,suggesting that the functional shift may coincide with the subsequent steps of chilling development.We further classified the potential regulatory components and concluded that ethylene may play the crucial role in chilling development in this non-climacteric fruit.To cope with complex events,53 upregulated transcription factors represented regulatory candidates.Within these,the AP2-EREBP,C2H2 and AS2 gene family were overrepresented.Cold storage also causes alterations in various metabolic pathways;a keen interest is paid in deciphering expression changes of soluble sugar genes as increased evidence that soluble sugars act as both osmolytes and metabolite signal molecules.Our results will likely facilitate further studies in this field with promising genetic candidates during chilling. | Andan Zhu Wenyun Li Junli Ye Xiaohua Sun Yuduan Ding Yunjiang Cheng Xiuxin Deng | 2011 | Journal of Integrative Plant Biology2011，53，5： | 3 |
| 5 | Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo显示文摘Self-incompatibility(SI)substantially restricts the yield and quality of citrus.Therefore,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for citrus.Here,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S haplotypes.Coupled with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase promoter.Our data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional regulation.This work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes. | ianbing Hu Qiang Xu Chenchen Liu Binghao Liu Chongling Deng Chuanwu Chen Zhuangmin Wei Muhammad Husnain Ahmad Kang Peng Hao Wen Xiangling Chen Peng Chen Robert MLarkin Junli Ye Xiuxin Deng Lijun Chai | 2021 | Horticulture Research2021，8，1： | 2 |
| 6 | Multiomics-based dissection of citrus flavonoid metabolism using a Citrus reticulata × Poncirus trifoliata population显示文摘Deciphering the genetic basis of plant secondary metabolism will provide useful insights for genetic improvement and enhance our fundamental understanding of plant biological processes.Although citrus plants are among the most important fruit crops worldwide,the genetic basis of secondary metabolism in these plants is largely unknown.Here,we use a high-density linkage map to dissect large-scale flavonoid metabolic traits measured in different tissues(young leaf,old leaf,mature pericarp,and mature pulp)of an F_(1) pseudo-testcross citrus population.We detected 80 flavonoids in this population and identified 138 quantitative trait loci(QTLs)for 57 flavonoids in these four tissues.Based on transcriptional profiling and functional annotation,twenty-one candidate genes were identified,and one gene encoding flavanone 3-hydroxylase(F3H)was functionally verified to result in naturally occurring variation in dihydrokaempferol content through genetic variations in its promoter and coding regions.The abundant data resources collected for diverse citrus germplasms here lay the foundation for complete characterization of the citrus flavonoid biosynthetic pathway and will thereby promote efficient utilization of metabolites in citrus quality improvement. | Jiaolin Mou Zhehui Zhang Haiji Qiu Yang Lu Xiang Zhu Ziquan Fan Qinghua Zhang Junli Ye Alisdair R.Fernie Yunjiang Cheng Xiuxin Deng Weiwei Wen | 2021 | Horticulture Research2021，8，1： | 2 |
| 7 | Determination of formaldehyde in squid by high - performance liquid chromatography显示文摘 | Jianrong Li Junli Zhu Lifang Ye | | 0，，1： | 1 |
| 8 | Structural variation and parallel evolution of apomixis in citrus during domestication and diversification显示文摘Apomixis,or asexual seed formation,is prevalent in Citrinae via a mechanism termed nucellar or adventitious embryony.Here,multiple embryos of a maternal genotype form directly from nucellar cells in the ovule and can outcompete the developing zygotic embryo as they utilize the sexually derived endosperm for growth.Whilst nucellar embryony enables the propagation of clonal plants of maternal genetic constitution,it is also a barrier to effective breeding through hybridization.To address the genetics and evolution of apomixis in Citrinae,a chromosome-level genome of the Hongkong kumquat(Fortunella hindsii)was assembled following a genome-wide variation map including structural variants(SVs)based on 234 Citrinae accessions.This map revealed that hybrid citrus cultivars shelter genome-wide deleterious mutations and SVs into heterozygous states free from recessive selection,which may explain the capability of nucellar embryony in most cultivars during Citrinae diversification.Analyses revealed that parallel evolution may explain the repeated origin of apomixis in different genera of Citrinae.Within Fortunella,we found that apomixis of some varieties originated via introgression.In apomictic Fortunella,the locus associated with apomixis contains the FhRWP gene,encoding an RWP-RK domain-containing protein previously shown to be required for nucellar embryogenesis in Citrus.We found the heterozygous SV in the FhRWP and CitRWP promoters from apomictic Citrus and Fortunella,due to either two or three miniature inverted transposon element(MITE)insertions.A transcription factor,FhARID,encoding an AT-rich interaction domain-containing protein binds to the MITEs in the promoter of apomictic varieties,which facilitates induction of nucellar embryogenesis.This study provides evolutionary genomic and molecular insights into apomixis in Citrinae and has potential ramifications for citrus breeding. | Nan Wang Xietian Song Junli Ye Siqi Zhang Zhen Cao Chenqiao Zhu Jianbing Hu Yin Zhou Yue Huang Shuo Cao Zhongjie Liu Xiaomeng Wu Lijun Chai Wenwu Guo Qiang Xu Brandon S.Gaut Anna M.G.Koltunow Yongfeng Zhou Xiuxin Deng | 2022 | National Science Review2022，9，10： | 1 |
| 9 | Effect of Purple Sweet Potato Anthocyanins on β-Amyloid-Mediated PC-12 Cells Death by Inhibition of Oxidative Stress显示文摘 | Junli Ye Xiangjun Meng Chunling Yan Chunbo Wang | 2010 | Neurochemical Research2010，，3： | 1 |
| 10 | Determination of Formaldehyde in Squid by high - performance liquid chromatography显示文摘 | LI Jianrong ZHU Junli YE Lifang | 2007 | Asia Pac J Clin Nutr2007，16，1： | 1 |
| 11 | Marine lysozyme from a marine bacterium that inhibits angiogenesis and tumor growth显示文摘 | Junli Ye Chunbo Wang Xuehong Chen Shenbo Guo Mi Sun | 2008 | Applied Microbiology and Biotechnology2008，，6： | 1 |
| 12 | Highly selective electrocatalytic Cl− oxidation reaction by oxygen-modified cobalt nanoparticles immobilized carbon nanofibers for coupling with brine water remediation and H2 production显示文摘Combining the H2 production with brine remediation is regarded as a sustainable approach to achieving clean H2 energy. However, designing stable Cl− oxidation reaction (COR) electrocatalyst is the key to realize this route. Herein, a type of oxygen-modified Co nanoparticles anchored graphitic carbon nanofibers catalyst (Co/GCFs) was synthesized through a two-step strategy of adsorption and pyrolysis. The Co/GCFs-2.4 exhibits high selectivity and stability for COR at neutral electrolyte. It is worth noting that unlike the water oxidation, the chemical valence of cobalt has not changed during the COR. Further results demonstrated that the oxygen-modified Co nanoparticles provide active sites for selective COR, meanwhile, the graphitic carbon gives rise to strong catalytic stability. Thanks to the superior COR and H2 production activity of Co/GCFs-2.4, a two-electrode brine electrocatalysis system employing Co/GCFs-2.4 as both cathode and anode for H2 production exhibited robust stability, efficient and high Faraday efficiency (98%-100%). We propose that this work provides a novel strategy for designing efficient and stable catalysts with electrocatalytic COR and HER activities at neutral brine water for practically coupling with H2 production by water electrolysis and brine water remediation. | Qizhong Xiong Xian Zhang Qipeng Cheng Guoqiang Liu Gang Xu Junli Li Xinxin Ye Hongjian Gao | 2021 | Nano Research2021，14，5： | 1 |
| 13 | Transcriptome and co-expression network analyses provide insights into fruit shading that enhances carotenoid accumulation in pomelo(Citrus grandis)显示文摘Carotenoids are indispensable for human health,and citrus fruit are a crucial source of dietary carotenoids.Bagging,an important orchard practice to enhance fruit economic value,is widely used in many horticultural crops,including citrus fruit.The bagged‘Majiayou’pomelo(Citrus grandis)produces vivid deeper red pulp,a fantastic agronomic trait,but the underlying molecular regulatory mechanism remains largely unexplored.Here,the enhancement of carotenoids,especially lycopene,was confirmed by HPLC analysis of carotenoids in the pulp of bagged fruit and controls.qRT-PCR analysis of the 14 carotenoid pathway genes further revealed that upregulated PSY and downregulated CCD1 expression in bagged fruit could directly enhance the accumulation of carotenoids.In addition,RNA-seq analysis identified 311 differentially expressed genes(DEGs)in the bagged fruit and controls in five developmental stages.Weighted gene co-expression network analysis(WGCNA)identified 13 critical candidate genes among the DEGs,which are closely associated with lycopene accumulation.The underlying regulatory mechanism of these candidate genes on the transcription of carotenoid pathway genes in the bagged fruit was discussed.Considering that the candidate genes were involved in the corresponding metabolic pathways,the increase in sucrose content and decrease in ABA in bagged fruit were also identified,implying that these candidate genes may be indirectly related to carotenoid enhancement in pulp by regulating phytohormones,primary metabolism,and stress responses.The results provide new insights into the potential regulatory mechanism of lycopene enhancement in the pulp of bagged‘Majiayou’pomelo,facilitating breeding and orchard management efforts to improve the nutritional quality and esthetic value of citrus,and perhaps other fruit crops. | Qihang Jiang Junli Ye Kaijie Zhu Fangfang Wu Lijun Chai Qiang Xu Xiuxin Deng | 2022 | Horticultural Plant Journal2022，8，4： | 1 |
| 14 | Panicle-3D: A low-cost 3D-modeling method for rice panicles based on deep learning, shape from silhouette, and supervoxel clustering显示文摘Self-occlusions are common in rice canopy images and strongly influence the calculation accuracies of panicle traits. Such interference can be largely eliminated if panicles are phenotyped at the 3 D level.Research on 3 D panicle phenotyping has been limited. Given that existing 3 D modeling techniques do not focus on specified parts of a target object, an efficient method for panicle modeling of large numbers of rice plants is lacking. This paper presents an automatic and nondestructive method for 3 D panicle modeling. The proposed method integrates shoot rice reconstruction with shape from silhouette, 2 D panicle segmentation with a deep convolutional neural network, and 3 D panicle segmentation with ray tracing and supervoxel clustering. A multiview imaging system was built to acquire image sequences of rice canopies with an efficiency of approximately 4 min per rice plant. The execution time of panicle modeling per rice plant using 90 images was approximately 26 min. The outputs of the algorithm for a single rice plant are a shoot rice model, surface shoot rice model, panicle model, and surface panicle model, all represented by a list of spatial coordinates. The efficiency and performance were evaluated and compared with the classical structure-from-motion algorithm. The results demonstrated that the proposed method is well qualified to recover the 3 D shapes of rice panicles from multiview images and is readily adaptable to rice plants of diverse accessions and growth stages. The proposed algorithm is superior to the structure-from-motion method in terms of texture preservation and computational efficiency. The sample images and implementation of the algorithm are available online. This automatic, cost-efficient, and nondestructive method of 3 D panicle modeling may be applied to high-throughput 3 D phenotyping of large rice populations. | Dan Wu Lejun Yu Junli Ye Ruifang Zhai Lingfeng Duan Lingbo Liu Nai Wu Zedong Geng Jingbo Fu Chenglong Huang Shangbin Chen Qian Liu Wanneng Yang | 2022 | The Crop Journal2022，10，5： | 1 |
| 15 | A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function显示文摘Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit. | Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu Chongling Deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin Deng Yunliu Zeng | 2018 | Horticulture Research2018，5，1： | 1 |
| 16 | Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+cells in pancreatic cancer显示文摘 | Ye Zhang Jishu Wei Hui Wang Xiaofeng Xue Yong An Dong Tang Zhongxu Yuan Feitong Wang Junli Wu Jingjing Zhang Yi Miao | 2012 | Oncology Reports2012，，5： | 1 |
| 17 | Citrus β-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of β-carotene and compensates for BCH1 in citrus carotenoid metabolism显示文摘Abstract As an essential horticultural crop,Citrus has carotenoid diversity,which affects its aesthetic and nutritional values.β,β-Xanthophylls are the primary carotenoids accumulated in citrus fruits,and non-heme di-iron carotene hydroxylase(BCH)enzymes are mainly responsible forβ,β-xanthophyll synthesis.Previous studies have focused on the hydroxylation of BCH1,but the role of its paralogous gene in citrus,BCH2,remains largely unknown.In this study,we revealed theβ-hydroxylation activity of citrus BCH2(CsBCH2)for the first time through the functional complementation assay using Escherichia coli,although CsBCH2 exhibited a lower activity in hydroxylatingβ-carotene intoβ-cryptoxanthin than citrus BCH1(CsBCH1).Our results showed that overexpression of CsBCH2 in citrus callus increased xanthophyll proportion and plastoglobule size with feedback regulation of carotenogenic gene expression.This study revealed the distinct expression patterns and functional characteristics of two paralogous genes,CsBCH1 and CsBCH2,and illustrated the backup compensatory role of CsBCH2 for CsBCH1 in citrus xanthophyll biosynthesis.The independent function of CsBCH2 and its cooperative function with CsBCH1 inβ-cryptoxanthin biosynthesis suggested the potential of CsBCH2 to be employed for expanding the synthetic biology toolkit in carotenoid engineering。 | Yingzi Zhang Jiajing Jin Shenchao Zhu Quan Sun Yin Zhang Zongzhou Xie Junli Ye Xiuxin Deng | 2023 | Horticulture Research2023，10，3： | 0 |
| 18 | Transcription factor CsTT8 promotes fruit coloration by positively regulating the methylerythritol 4-phosphate pathway and carotenoid biosynthesis pathway in citrus (Citrus spp.)显示文摘Carotenoids directly influence citrus fruit color and nutritional value,which is critical to consumer acceptance.Elucidating the potential molecular mechanism underlying carotenoid metabolism is of great importance for improving fruit quality.Despite the well-established carotenoid biosynthetic pathways,the molecular regulatory mechanism underlying carotenoid metabolism remains poorly understood.Our previous studies have reported that the Myc-type basic helix-loop-helix(bHLH)transcription factor(TF)regulates citrus proanthocyanidin biosynthesis.Transgenic analyses further showed that overexpression of CsTT8 could significantly promote carotenoid accumulation in transgenic citrus calli,but its regulatory mechanism is still unclear.In the present study,we found that overexpression of CsTT8 enhances carotenoid content in citrus fruit and calli by increasing the expression of CsDXR,CsHDS,CsHDR,CsPDS,CsLCYE,CsZEP,and CsNCED2,which was accompanied by changes in the contents of abscisic acid and gibberellin.The in vitro and in vivo assays indicated that CsTT8 directly bound to the promoters of CsDXR,CsHDS,and CsHDR,the keymetabolic enzymes of the methylerythritol 4-phosphate(MEP)pathway,thus providing precursors for carotenoid biosynthesis and transcriptionally activating the expression of these three genes.In addition,CsTT8 activated the promoters of four key carotenoid biosynthesis pathway genes,CsPDS,CsLCYE,CsZEP,and CsNCED2,directly promoting carotenoid biosynthesis.This study reveals a novel network of carotenoid metabolism regulated by CsTT8.Our findings will contribute to manipulating carotenoid metabolic engineering to improve the quality of citrus fruit and other crops. | Quan Sun Zhengchen He Ranran Wei Yingzi Yin Junli Ye Lijun Chai Zongzhou Xie Wenwu Guo Juan Xu Yunjiang Cheng Qiang Xu Xiuxin Deng | 2023 | Horticulture Research2023，10，11： | 0 |
| 19 | NEW INSIGHTS INTO THE PHYLOGENY AND SPECIATION OF KUMQUAT (FORTUNELLA SPP.) BASED ON CHLOROPLAST SNP, NUCLEAR SSR AND WHOLE-GENOME SEQUENCING显示文摘Kumquat(Fortunella spp.)is a fruit and ornamental crop worldwide due to the palatable taste and high ornamental value of its fruit.Although Fortunella is classified into the economically important true citrus fruit tree group together with Citrus and Poncirus,few studies have been focused on its evolutionary scenario.In this study,analysis of five chloroplast loci and 47 nuclear microsatellites(nSSR)loci from 38 kumquat and 10 citrus accessions revealed the independent phylogeny of Fortunella among citrus taxa,and that Fortunella mainly comprises two populations:CUL,cultivated Fortunella spp.(F.margarita,F.crassifolia and F.japonica);and HK,wild Hong Kong kumquat(Fortunella hindsii).Genomic analysis based on whole-genome SNPs indicated that the allele frequency of both pupations deviated from the neutral selection model,suggesting directional selection was a force driving their evolutions.CUL exhibited lower genomic diversity and higher linkage strength than HK,suggesting artificial selection involved in its origin.A high level of genetic differentiation(Fst=0.364)was detected and obviously asynchronous demographic changes were observed between CUL and HK.Based on these results,a new hypothesis for the speciation of Fortunella is proposed. | Chenqiao ZHU Peng CHEN Junli YE Hang LI Yue HUANG Xiaoming YANG Chuanwu CHEN Chenglei ZHANG Yuantao XU Xiaoli WANG Xiang YAN Guangzhou DENG Xiaolin JIANG Nan WANG Hongxing WANG Quan SUN Yun LIU Di FENG Min YU Xietian SONG Zongzhou XIE Yunliu ZENG Lijun CHAI Qiang XU Chongling DENG Yunjiang CHENG Xiuxin DENG | 2022 | Frontiers of Agricultural Science and Engineering2022，9，4： | 0 |
| 20 | A new contribution to the taxonomy and phylogeny of the ciliate genus Spirostomum(Alveolata,Ciliophora,Heterotrichea),with comprehensive descriptions of two species from wetlands in China显示文摘Species of the ciliate genus Spirostomum Ehrenberg,1834 are distributed worldwide and have a research history spanning more than two centuries.However,species delimitation and phylogenetic relationships within this genus are still uncertain due to the paucity of stable morphologic characters for species separation and the unavailability of accompanying morphological data for most molecular sequences in public databases.In the present study,S.yagiui Shigenaka,1959(three populations)and S.caudatum(Müller,1786)Delphy,1939(one population)were investigated using morphological and molecular methods for the first time in China.Detailed morphological data for the two species were documented,and improved diagnoses were supplied based on a combination of previous studies and the current work.It should be highlighted that there were three different atypical morphotypes identified in a Ningbo population of S.yagiui which may represent various stages in conjugative reproduction.Molecular phylogenies based on 18S,ITS1-5.8S-ITS2,and 28S rRNA gene sequences show that the genus Spirostomum is monophyletic,however,the internal relationships inferred from different genes were poorly resolved but suggest that the species with a moniliform macronucleus comprise an early-diverging clade within this genus.Finally,the global distribution of Spirostomum is summarized based on previous and present studies. | Yong Chi Zhe Wang Tingting Ye Ya Wang Junli Zhao Weibo Song William A.Bourland Xiangrui Chen | 2022 | Water Biology and Security2022，1，2： | 0 |
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