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1Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant?显示文摘Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro.DOU Hai Wei TIAN Xiu Jun XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei 2020Biomedical and Environmental Sciences2020,33,12:9
2Dietary high-fat lard intake induces thyroid dysfunction and abnormal morphology in rats显示文摘Shan-shan SHAO Yuan-fei ZHAO Yong-feng SONG Chao XU Jian-mei YANG Shi-meng XUAN Hui-li YAN Chun-xiao YU Meng ZHAO Jin XU Jia-jun ZHAO 2014Acta Pharmacologica Sinica2014,35,11:9
3Cell membrane-covered nanoparticles as biomaterials显示文摘Surface engineering of synthetic carriers is an essential and important strategy for drug delivery in vivo.However,exogenous properties make synthetic nanosystems invaders that easily trigger the passive immune clearance mechanism,increasing the retention effect caused by the reticuloendothelial systems and bioadhesion,finally leading to low therapeutic efficacy and toxic effects.Recently,a cell membrane cloaking technique has been reported as a novel interfacing approach from the biological/immunological perspective,and has proved useful for improving the performance of synthetic nanocarriers in vivo.After cell membrane cloaking,nanoparticles not only acquire the physiochemical properties of natural cell membranes but also inherit unique biological functions due to the presence of membrane-anchored proteins,antigens,and immunological moieties.The derived biological properties and functions,such as immunosuppressive capability,long circulation time,and targeted recognition integrated in synthetic nanosystems,have enhanced their potential in biomedicine in the future.Here,we review the cell membrane-covered nanosystems,highlight their novelty,introduce relevant biomedical applications,and describe the future prospects for the use of this novel biomimetic system constructed from a combination of cell membranes and synthetic nanomaterials.Mingjun Xuan Jingxin Shao Junbai Li 2019National Science Review2019,6,3:9
4Single-cell Immune Landscape of Human Recurrent Miscarriage显示文摘t Successful pregnancy in placental mammals substantially depends on the establishment of maternal immune tolerance to the semi-allogenic fetus.Disorders in this process are tightly associated with adverse pregnancy outcomes including recurrent miscarriage(RM).However,an indepth understanding of the systematic and decidual immune environment in RM remains largely lacking.In this study,we utilized single-cell RNA-sequencing(scRNA-seq)to comparably analyze the cellular and molecular signatures of decidual and peripheral leukocytes in normal and unexplained RM pregnancies at the early stage of gestation.Integrative analysis identifies 22 distinct cell clusters in total,and a dramatic difference in leukocyte subsets and molecular properties in RM cases is revealed.Specifically,the cytotoxic properties of CD8^(+)effector T cells,nature killer(NK),and mucosal-associated invariant T(MAIT)cells in peripheral blood indicates apparently enhanced pro-inflammatory status,and the population proportions and ligand–receptor interactions of the decidual leukocyte subsets demonstrate preferential immune activation in RM patients.The molecular features,spatial distribution,and the developmental trajectories of five decidual NK(dNK)subsets have been elaborately illustrated.In RM patients,a dNK subset that supports embryonic growth is diminished in proportion,while the ratio of another dNK subset with cyto toxic and immune-active signature is significantly increased.Notably,a unique pro-inflammatory CD56^(+)CD16^(+)dNK subset substantially accumulates in RM decidua.These findings reveal a comprehensive cellular and molecular atlas of decidual and peripheral leukocytes in human early pregnancy and provide an in-depth insight into the immune pathogenesis for early pregnancy loss.Feiyang Wang Wentong Jia Mengjie Fan Xuan Shao Zhilang Li Yongjie Liu Yeling Ma Yu-Xia Li Rong Li Qiang Tu Yan-Ling Wang 2021Genomics, Proteomics & Bioinformatics2021,19,2:8
5Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes.MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 2020Biomedical and Environmental Sciences2020,33,12:8
6H2 strain attenuated live hepatitis A vaccines:protective efficacy in a hepatitis A outbreak显示文摘AIM To investigate the protective efficacy ofH2 strain attenuated live hepatitis A vaccines(H2-strain vaccines)in hepatitis A(HA)outbreaks.METHODS With the permission of theirparents,5551 pre-school and grade 1-3 primaryschool children were inoculated with 1 dose(106.5TCID50)of H2-strain vaccines in anonrandomized,controlled trial conducted inFucheng County,Hebei Province in May 1997.Another 6485 children in the same grades andcompatible in gender and age were enrolled ascontrols.Epidemiological and serological surveywas conducted to evaluate the protectiveefficacy of the vaccines.ELISA was used todetect serum IgM anti-HAV.RESULTS HA outbreak started in early May1998,peaked in the middle of the same month,and lasted about 80 days.Overall 302 HA caseswere found,192(53.58%)were 5-9 years old.One vaccinee and 25 control cases were found tohave hepatitis A,which account for 0.28%(1/356)and 5.92%(25/422)of all vaccinees andcontrols in the 14 villages,respectively.The protective efficacy of vaccines was 95.27%(95% Cl:85.83%-104.72%).In subjects testedfor anti-HAV IgM from 13 villages,1(0.40%)overt and 11(4.06%)asymptomatic HAV caseswere found in 271 vaccinees,but 21(6.69%)ofovert and asymptomatic ones were found in 314controls.CONCLUSION H2 strain vaccines were excellent in preventing overt hepatitis A, but not so effective in preventing asymptomatic hepatitis A virus infection. A booster dose might be needed to get permanent reliable immunity.Yu Liang Zhao Zong Da Meng Zhi Yi Xu Jun Jie Guo Shao Ai Chai Cheng Gang Duo Xuan Yi Wang Jin Feng Yao Hong Bin Liu Shun Xiang Qi Hui Bin Zhu 2000World Journal of Gastroenterology2000,6,6:7
7Poly(ADP-ribosyl)ation of Apoptosis Antagonizing Transcription Factor Involved in Hydroquinone-Induced DNA Damage Response显示文摘The molecular mechanism of DNA damage induced by hydroquinone(HQ)remains unclear.Poly(ADP-ribose)polymerase-1(PARP-1)usually works as a DNA damage sensor,and hence,it is possible that PARP-1 is involved in the DNA damage response induced by HQ.In TK6 cells treated with HQ,PARP activity as well as the expression of apoptosis antagonizingling xiao xuan liu jia xian yun lin du yu jun chen shao qian chen jia long tang huan wen liu lin hua 2016Biomedical and Environmental Sciences2016,29,1:6
8Glutamate Impairs Mitochondria Aerobic Respiration Capacity and Enhances Glycolysis in Cultured Rat Astrocytes显示文摘Objective To study the effect of glutamate on metabolism, shifts in glycolysis and lactate release in rat astrocytes. Methods After 10 days, secondary cultured astrocytes were treated with 1 mmol/L glutamate for 1 h, and the oxygen consumption rates(OCR) and extra cellular acidification rate(ECAR) was analyzed using a Seahorse XF 24 Extracellular Flux Analyzer. Cell viability was then evaluated by MTT assay. Moreover, changes in extracellular lactate concentration induced by glutamate were tested with a lactate detection kit. Results Compared with the control group, treatment with 1 mmol/L glutamate decreased the astrocytes' maximal respiration and spare respiratory capacity but increased their glycolytic capacity and glycolytic reserve. Further analysis found that 1-h treatment with different concentrations of glutamate(0.1-1 mmol/L) increased lactate release from astrocytes, however the cell viability was not affected by the glutamate treatment. Conclusion The current study provided direct evidence that exogenous glutamate treatment impaired the mitochondrial respiration capacity of astrocytes and enhanced aerobic glycolysis, which could be involved in glutamate injury or protection mechanisms in response to neurological disorders.YAN Xu SHI Zhong Fang XU Li Xin LI Jia Xin WU Min WANG Xiao Xuan JIA Mei DONG Li Ping YANG Shao Hua YUAN Fang 2017Biomedical and Environmental Sciences2017,30,1:6
9Real-time hyperspectral imaging for the in-field estimation of strawberry ripeness with deep learning显示文摘Strawberry is one of the popular fruits with numerous nutrients.The ripeness of this fruits was estimated using the hyperspectral imaging(HSI)system in field and laboratory conditions in this study.Strawberry at early ripe and ripe stageswere collected HSI data,coveredwavelength ranges from370 to 1015 nm.Spectral featurewavelengths were selected using the sequential feature selection(SFS)algorithm.Two wavelengths selected for field(530 and 604 nm)and laboratory(528 and 715 nm)samples,respectively.Then,reliability of such spectral featureswas validated based on support vectormachine(SVM)classifier.Performance of SVMclassification models had good resultswith receiver operating characteristic values for samples under both field and laboratory conditions higher than 0.95.Meanwhile,the spatial feature images were extracted from the spectral feature wavelength and the first three principal components for laboratory samples.Pretrained AlexNet convolutional neural network(CNN)was used to classify the early ripe and ripe strawberry samples,which obtained the accuracy of 98.6%for test dataset.The above results indicated real-time HSI system was promising for estimating strawberry ripeness under field and laboratory conditions,which could be a potential application technique for evaluating the harvesting time management for farmers and producers.Zongmei Gao Yuanyuan Shao Guantao Xuan Yongxian Wang Yi Liu Xiang Han 2020Artificial Intelligence in Agriculture2020,,1:6
10Efficacy of Lycium barbarum polysaccharide in adolescents with subthreshold depression: interim analysis of a randomized controlled study显示文摘Subthreshold depression is a highly prevalent condition in adolescents who are at high risk for developing major depressive disorder.In preclinical models of neurological and psychiatric diseases,Lycium barbarum polysaccharide(LBP)extracted from Goji berries had antidepressant effects including but not limited to anti-oxidative and anti-inflammatory properties.However,the effect of LBP on subthreshold depression is unclear.To investigate the clinical efficacy and safety of LBP for treating subthreshold depression in adolescents,we conducted a randomized,double-blind,placebo-controlled trial(RCT)with 29 adolescents with subthreshold depression recruited at The Fifth Affiliated Hospital of Guangzhou Medical University.The participants were randomly assigned to groups where they received either 300 mg LBP(LBP group,n=15,3 boys and 12 girls aged 15.13±2.17 years)or a placebo(placebo group,n=14,2 boys and 12 girls aged 15±1.71 years)for 6 successive weeks.Interim analyses revealed that the LBP group exhibited a greater change in Hamilton Depression Scale(HAMD-24)scores relative to the baseline and a higher remission rate(HAMD-24 total score≤7)at 6 weeks compared with the placebo group.Scores on the Beck Depression Inventory-II(BDI-II),Pittsburgh Sleep Quality Index(PSQI),Kessler Psychological Distress Scale(Kessler),and Screen for Child Anxiety-Related Emotional Disorders(SCARED)were similar between the LBP and placebo groups.No side effects related to the intervention were reported in either group.These results indicate that LBP administration reduced depressive symptoms in adolescents with subthreshold depression.Furthermore,LBP was well tolerated with no treatment-limiting adverse events.Clinical trials involving a larger sample size are needed to further confirm the anti-depressive effects of LBP in adolescents with subthreshold depression.This study was approved by the Medical Ethics Committee of the Fifth Affiliated Hospital of Guangzhou Medical University(Guangzhou,China;approval No.L2019-08)on April 4,2019 and was registered on ClinicalTrials.gov(identifier:NCT04032795)on July 25,2019.Xiaoyue Li Xuan Mo Tao Liu Robin Shao Kayla Teopiz Roger S.McIntyre Kwok-Fai So Kangguang Lin 2022Neural Regeneration Research2022,17,7:6
11The Mechanism and Influence of AKAP12 in Different Cancers显示文摘The development of cancer is a complex process that requires the participation of many factors, including mutations in genes, regulation of signaling pathways, disruption of homeostasis, and failure of self-monitoring mechanisms. Sufficient evidence has shown that the A kinase anchoring protein (AKAP) family plays an important role in signal transduction in cancer. AKAP12 and AKAP12-regulated signaling pathways are critically involved in the development and progression of a variety of tumors.WU Xuan WU Tong LI Ke LI Yuan HU Ting Ting WANG Wei Feng QIANG Su Jing XUE Shao Bo LIU Wei Wei 2018Biomedical and Environmental Sciences2018,31,12:5
12Structural variation during dog domestication: insights from gray wolf and dhole genomes显示文摘Several processes like phenotypic evolution, disease susceptibility and environmental adaptations, which fashion the domestication of animals, are largely attributable to structural variations(SVs) in the genome.Here, we present high-quality draft genomes of the gray wolf(Canis lupus) and dhole(Cuon alpinus) with scaffold N50 of 6.04 Mb and 3.96 Mb, respectively. Sequence alignment comprising genomes of three canid species reveals SVs specific to the dog, particularly 16 315 insertions, 2565 deletions, 443 repeats, 16 inversions and 15 translocations. Functional annotation of the dog SVs associated with genes indicates their enrichments in energy metabolisms, neurological processes and immune systems. Interestingly, we identify and verify at population level an insertion fully covering a copy of the AKR1 B1(Aldo-Keto Reductase Family 1 Member B) transcript. Transcriptome analysis reveals a high level of expression of the new AKR1 B1 copy in the small intestine and liver, implying an increase in de novo fatty acid synthesis and antioxidant ability in dog compared to gray wolf, likely in response to dietary shifts during the agricultural revolution. For the first time, we report a comprehensive analysis of the evolutionary dynamics of SVs during the domestication step of dogs. Our findings demonstrate that retroposition can birth new genes to facilitate domestication, and affirm the importance of large-scale genomic variants in domestication studies.Guo-Dong Wang Xiu-Juan Shao Bing Bai Junlong Wang Xiaobo Wang Xue Cao Yan-Hu Liu Xuan Wang Ting-Ting Yin Shao-Jie Zhang Yan Lu Zechong Wang Lu Wang Wenming Zhao Bing Zhang Jue Ruan Ya-Ping Zhang 2019National Science Review2019,6,1:3
13Perspective of energy transfer from light energy into biological energy显示文摘Energy has always been the most concerned topic in the world due to the large consumption. Various types of energy have been exploited and developed to enhance the output amount so that high requirements can be met. Like the hydro-energy, wind energy, and tidal energy, light energy as a renewable, clean, and widespread energy can be easily harvested. In microcosmic scale, some specific proteins and enzymes in green plants and bacteria play an important role in light harvest and energy conversion via photosynthesis. Inspired by the biomimetic sparks,these bioactive macromolecules and some artificially synthetic unites have been integrated together to improve the light-harvesting, and enhance their utilization efficiency. In this feature article, we primarily discuss that how to create the bio-inorganic hybrid energy converted system via biomimetic assembly strategy and artificially achieve the transformation from light into bioenergy, meanwhile highlight some promising works.Mingjun Xuan Jie Zhao Jingxin Shao Qi Li Junbai Li 2017Green Energy & Environment2017,2,1:2
14Determination of damaged wheat kernels with hyperspectral imaging analysis显示文摘Hyperspectral imaging was applied to classify the damaged wheat kernels and healthy kernels.The spectral information was extracted from damaged wheat kernels and healthy kernels samples.The effective wavelengths were obtained from spectral of 865-1711 nm by X-loadings of principal component analysis(PCA)and successive projection algorithm(SPA)method,respectively.Partial least square method(PLS)and least square-support vector machine(LS-SVM)were then used to build classification models on full spectral data and effective wavelengths dataset,respectively.The results showed that the classification accuracy of every LS-SVM model was the best,being 100%.While the accuracy of the PLS model was slightly lower,still over 97%.The confusion matrix showed that several damaged wheat kernels samples were misclassified as healthy samples,while all healthy samples were correctly classified.The overall results indicated that hyperspectral imaging could be used for discriminating the damaged wheat kernels and could provide a reference for detecting other grain kernels grading degrees.Further,this study can provide a research basis for the development of online or portable detectors on grain damaged kernels recognition,which will be beneficial for grain grading or post-harvest quality processing of other grains.Yuanyuan Shao Chong Gao Guantao Xuan Xuemei Gao Youqing Chen Zhichao Hu 2020International Journal of Agricultural and Biological Engineering2020,13,5:2
15One Step Fabrication of Co3O4-PPy Cathode for Lithium-O2 Batteries显示文摘在 Li-O 2 电池的阴极的常规文件夹被物理混合与催化剂和进行的碳支持绝缘,并且集成。这限制电子的转移,降级阴极结构由于胀大,并且最后导致能力褪色并且对 cycleability 有害。在这研究,第一次,我们认识到一出一步舞制作了公司 3 一个在原处形成的传导性的聚合物文件夹描绘的 O 4-PPy 阴极。功能的文件夹, polypyrrole (PPy ) ,通过电的方法被综合,它在 Li-O 2 电池充当了文件夹和支持的材料。基于 PPy 的传导性的性质,我们获得了公司 3 经由 CV 电极淀积的过程的 O 4-PPy@Ni 电极。这个方法导致公司 3 O 4 nanoparticles 和罐头简化空气阴极的准备过程。有公司 32 电池 > 为超过 30 的 O 4-PPy@Ni 阴极罐头解除 / 控告在 1000 mAh/g 的有限特定的能力下面骑车。有 PPy 的功能的文件夹的 Li-O 2 电池的 cycleability 和阻抗系列比有常规文件夹的电池优异。Xuan Shao Tao Zhang Zhaoyin Wen 2017Chinese Journal of Chemistry2017,35,1:2
16LncRNA LUCAT1 Activation Mediated by theDown-regulation of DNMT1 Is Involved inCell Apoptosis Induced by PM2.5显示文摘Particulate matter (PM), which is a greatenvironmental concern, has been classified as aGroup 1 human carcinogen by the InternationalAgency for Research on Cancer (IARC).Epidemiological and experimental studies haveindicated that chronic exposure to PM, especiallyPM2.5 (particles with an aerodynamic diameter lessthan 2.5 μm) may lead to an increase in lung cancerincidence.LING Xiao Xuan ZHANG Hai Qiao LIU Jia Xian LIU Zhi Dong PENG Jian Ming ZHANG Xiao Qing SHAO Jun Li CHEN Zhen Fa LIU Lin Hua 2018Biomedical and Environmental Sciences2018,31,8:2
17Enzymic colorimetry- based DNA chip: a rapid and accurate assay for detecting mu- tations for clarithromycin resistance in the 23S rRNA gene o5 Helicobacter pylori显示文摘Xuan SH Zhou YG Shao B 2009J Med Microbiol2009,58,11:1
18Macrophage cell Membrane camouflaged mesoporous silica nanocapsules for in vivo cancer therapy 显示文摘Xuan M Shao J Dai L 2015Adv Healthc Mater2015,4,11:1
19Programmable metallization cells based on amorphous La 0.79 Sr 0.21 MnO 3 thin films for memory applications显示文摘Dongqing Liu Nannan Wang Guang Wang Zhengzheng Shao Xuan Zhu Chaoyang Zhang Haifeng Cheng 2013Journal of Alloys and Compounds2013,,:1
20Two New Diterpenoids from Isodon rubescens显示文摘Two new diterpenoids taibairubescensin A (1) and B (2) have been isolated from Isodon rubescens. The structures of compound 1 and 2 were elucidated as 2β,3β-diacetoxy-11β,13α- dihydroxy-ent-kaur- 16-en- 15-one (1) and 3β, 11β-diacetoxy-2β,6α-dihydroxy-ent -kaur-16-en- 15 one (2) on the basis of spectroscopic analysis.Bao Lin LI Shao Nong CHEN Zhi Xian SHI Xuan TIAN Yao Zu CHEN 2000Chinese Chemical Letters2000,11,1:1
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